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The map label for this gene is purA [H]

Identifier: 218883829

GI number: 218883829

Start: 484738

End: 485766

Strand: Reverse

Name: purA [H]

Synonym: DKAM_0518

Alternate gene names: 218883829

Gene position: 485766-484738 (Counterclockwise)

Preceding gene: 218883844

Following gene: 218883828

Centisome position: 35.58

GC content: 48.98

Gene sequence:

>1029_bases
GTGAGGGATATGCCCTCTCTCATCATAGTAGGAGGATTTTTCGGGGATGAGGGAAAAGGCAAGATAGCAGCCTACCTGGC
CCTCAGCGATAAGCCTGTAATAATTGTTAGAACAGGCTCGATCAACGCTGGTCACACCGTTGTGTTTAATGGAAAAACAT
TCAAGCTCAGAGCGGTCCCAAGCGGCTTTATCTCCAGGGATTCACGTCTTTTAATAGCTCCCGGAGCCCTAGTAAAATTG
GATATCCTATTCAAGGAGATAACCGAGACAGGTACAAGAGGCAGGGTTTTCCTGGATTATAACACAGGGATCATCGAGGA
ACGCCATGTCGAAGCAGAGCTGGCAGACCAGTACTTCTCGCAGGTAATTGGTTCAACACTACAGGGAGTTGGTGCAGCGA
TGATCGATAGGGTTGCTAGAAGACTTAAACTAGCGAGAGACTTCGAGGAGTTGAAGGAGACAACTATAGATGTCCCTGGG
GCCGTTAACGAGGCTTTGTCGAGGGGTGAATTAGTACATGTGGAGGGAACCCAAGGCTCGTTCCTGAGCCTGTACCACGG
CACATACCCCTATGTAACTAGTAGGGATACCACGGCATCGGCTGTAGCATCAGAGGTTGGACTAGGCCCTAAGCATGTGG
ATGATGTAGTAGTCGTGTTTAAAGCCTACATCACGAGAGTTGGAAACGGACCATTACCAGGTGAGCTAAGTCACAGTGAA
ACCTTAAAGCTAGGATGGGTTGAAGAGGCGACGGTGACACGTAGAATCAGGAGGGTGGCACCTTTCAGTATTGAACTAGC
CAGAAGAGCCATAATGCTTAACTCAGCAACACAAATAGCCTTAACAAAGCTCGACGTGCTCTACAGGGAGTCAAGGGGGT
GCAGAGAGTGGTCGTGTCTACCAGTTGAAGCAAGAAAATGGGTGGAGCACGTGGAGGATTCATTAAAGACCCCGGTATCG
CTGATAGGAACATGTGAAGAAGTGAACTGCGTGATCGATAGGAGAAGAGAGCTCGGATTACTGAGGTGA

Upstream 100 bases:

>100_bases
TAAAACGATACCGGCCCCAGTATTACCTTCATTAATGTTAACCAGTATCATTGACGCCCTAGGCAATTATTTATTTACAT
AGGAAACATTAAGCTCACGG

Downstream 100 bases:

>100_bases
AGCCCTTGAGTAAATATGATGTCGTGATAGTTGGCGGCGGACCCGCCGGATTATTCGCTGCATACGAGCTATCATTTCAT
ACAGGTAAGCTGAGAGTGCT

Product: adenylosuccinate synthetase

Products: NA

Alternate protein names: AMPSase; AdSS; IMP--aspartate ligase [H]

Number of amino acids: Translated: 342; Mature: 342

Protein sequence:

>342_residues
MRDMPSLIIVGGFFGDEGKGKIAAYLALSDKPVIIVRTGSINAGHTVVFNGKTFKLRAVPSGFISRDSRLLIAPGALVKL
DILFKEITETGTRGRVFLDYNTGIIEERHVEAELADQYFSQVIGSTLQGVGAAMIDRVARRLKLARDFEELKETTIDVPG
AVNEALSRGELVHVEGTQGSFLSLYHGTYPYVTSRDTTASAVASEVGLGPKHVDDVVVVFKAYITRVGNGPLPGELSHSE
TLKLGWVEEATVTRRIRRVAPFSIELARRAIMLNSATQIALTKLDVLYRESRGCREWSCLPVEARKWVEHVEDSLKTPVS
LIGTCEEVNCVIDRRRELGLLR

Sequences:

>Translated_342_residues
MRDMPSLIIVGGFFGDEGKGKIAAYLALSDKPVIIVRTGSINAGHTVVFNGKTFKLRAVPSGFISRDSRLLIAPGALVKL
DILFKEITETGTRGRVFLDYNTGIIEERHVEAELADQYFSQVIGSTLQGVGAAMIDRVARRLKLARDFEELKETTIDVPG
AVNEALSRGELVHVEGTQGSFLSLYHGTYPYVTSRDTTASAVASEVGLGPKHVDDVVVVFKAYITRVGNGPLPGELSHSE
TLKLGWVEEATVTRRIRRVAPFSIELARRAIMLNSATQIALTKLDVLYRESRGCREWSCLPVEARKWVEHVEDSLKTPVS
LIGTCEEVNCVIDRRRELGLLR
>Mature_342_residues
MRDMPSLIIVGGFFGDEGKGKIAAYLALSDKPVIIVRTGSINAGHTVVFNGKTFKLRAVPSGFISRDSRLLIAPGALVKL
DILFKEITETGTRGRVFLDYNTGIIEERHVEAELADQYFSQVIGSTLQGVGAAMIDRVARRLKLARDFEELKETTIDVPG
AVNEALSRGELVHVEGTQGSFLSLYHGTYPYVTSRDTTASAVASEVGLGPKHVDDVVVVFKAYITRVGNGPLPGELSHSE
TLKLGWVEEATVTRRIRRVAPFSIELARRAIMLNSATQIALTKLDVLYRESRGCREWSCLPVEARKWVEHVEDSLKTPVS
LIGTCEEVNCVIDRRRELGLLR

Specific function: Plays an important role in the de novo pathway of purine nucleotide biosynthesis. Catalyzes the first commited step in the biosynthesis of AMP from IMP [H]

COG id: COG0104

COG function: function code F; Adenylosuccinate synthase

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the adenylosuccinate synthetase family [H]

Homologues:

Organism=Homo sapiens, GI22748717, Length=424, Percent_Identity=25.4716981132075, Blast_Score=122, Evalue=4e-28,
Organism=Homo sapiens, GI34577063, Length=414, Percent_Identity=26.3285024154589, Blast_Score=122, Evalue=5e-28,
Organism=Homo sapiens, GI40316944, Length=394, Percent_Identity=24.3654822335025, Blast_Score=103, Evalue=2e-22,
Organism=Escherichia coli, GI1790620, Length=364, Percent_Identity=34.8901098901099, Blast_Score=165, Evalue=4e-42,
Organism=Caenorhabditis elegans, GI25146553, Length=425, Percent_Identity=30.1176470588235, Blast_Score=158, Evalue=3e-39,
Organism=Caenorhabditis elegans, GI25146555, Length=425, Percent_Identity=30.1176470588235, Blast_Score=158, Evalue=4e-39,
Organism=Saccharomyces cerevisiae, GI6324109, Length=419, Percent_Identity=25.5369928400955, Blast_Score=117, Evalue=3e-27,
Organism=Drosophila melanogaster, GI21356033, Length=331, Percent_Identity=30.5135951661631, Blast_Score=134, Evalue=8e-32,

Paralogues:

None

Copy number: 940 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR018220
- InterPro:   IPR001114 [H]

Pfam domain/function: PF00709 Adenylsucc_synt [H]

EC number: =6.3.4.4 [H]

Molecular weight: Translated: 37651; Mature: 37651

Theoretical pI: Translated: 7.99; Mature: 7.99

Prosite motif: PS01266 ADENYLOSUCCIN_SYN_1

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.2 %Cys     (Translated Protein)
1.2 %Met     (Translated Protein)
2.3 %Cys+Met (Translated Protein)
1.2 %Cys     (Mature Protein)
1.2 %Met     (Mature Protein)
2.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MRDMPSLIIVGGFFGDEGKGKIAAYLALSDKPVIIVRTGSINAGHTVVFNGKTFKLRAVP
CCCCCCEEEEEEEECCCCCCEEEEEEEECCCCEEEEEECCCCCCCEEEECCCEEEEEECC
SGFISRDSRLLIAPGALVKLDILFKEITETGTRGRVFLDYNTGIIEERHVEAELADQYFS
CHHHCCCCEEEECCCCEEHHHHHHHHHHCCCCCCEEEEEECCCCCCHHHHHHHHHHHHHH
QVIGSTLQGVGAAMIDRVARRLKLARDFEELKETTIDVPGAVNEALSRGELVHVEGTQGS
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHCCCCEEEEECCCCC
FLSLYHGTYPYVTSRDTTASAVASEVGLGPKHVDDVVVVFKAYITRVGNGPLPGELSHSE
EEEEECCCCCEEECCCCHHHHHHHHHCCCCHHHHHHHHHHHHHHHHCCCCCCCCCCCCCC
TLKLGWVEEATVTRRIRRVAPFSIELARRAIMLNSATQIALTKLDVLYRESRGCREWSCL
EEEECCCHHHHHHHHHHHHCCCHHHHHHHHHHHCCCHHHHHHHHHHHHHHCCCCCCCCCC
PVEARKWVEHVEDSLKTPVSLIGTCEEVNCVIDRRRELGLLR
CHHHHHHHHHHHHHHCCCHHHHCCHHHHHHHHHHHHHCCCCC
>Mature Secondary Structure
MRDMPSLIIVGGFFGDEGKGKIAAYLALSDKPVIIVRTGSINAGHTVVFNGKTFKLRAVP
CCCCCCEEEEEEEECCCCCCEEEEEEEECCCCEEEEEECCCCCCCEEEECCCEEEEEECC
SGFISRDSRLLIAPGALVKLDILFKEITETGTRGRVFLDYNTGIIEERHVEAELADQYFS
CHHHCCCCEEEECCCCEEHHHHHHHHHHCCCCCCEEEEEECCCCCCHHHHHHHHHHHHHH
QVIGSTLQGVGAAMIDRVARRLKLARDFEELKETTIDVPGAVNEALSRGELVHVEGTQGS
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHCCCCEEEEECCCCC
FLSLYHGTYPYVTSRDTTASAVASEVGLGPKHVDDVVVVFKAYITRVGNGPLPGELSHSE
EEEEECCCCCEEECCCCHHHHHHHHHCCCCHHHHHHHHHHHHHHHHCCCCCCCCCCCCCC
TLKLGWVEEATVTRRIRRVAPFSIELARRAIMLNSATQIALTKLDVLYRESRGCREWSCL
EEEECCCHHHHHHHHHHHHCCCHHHHHHHHHHHCCCHHHHHHHHHHHHHHCCCCCCCCCC
PVEARKWVEHVEDSLKTPVSLIGTCEEVNCVIDRRRELGLLR
CHHHHHHHHHHHHHHCCCHHHHCCHHHHHHHHHHHHHCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 9389475 [H]