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The map label for this gene is speE [H]

Identifier: 218883614

GI number: 218883614

Start: 282493

End: 283353

Strand: Direct

Name: speE [H]

Synonym: DKAM_0303

Alternate gene names: 218883614

Gene position: 282493-283353 (Clockwise)

Preceding gene: 218883613

Following gene: 218883617

Centisome position: 20.69

GC content: 47.74

Gene sequence:

>861_bases
GTGTCCGGTGAGGAGGGATTATGCTTATTTGAACCAGCCGGTAGATCATTCAAGCTATTATTAAGGGTCCTCAAGGTATA
CGCTGTTGAGAAATCGCCATACCAGGAGATAATATTCGCCGAGCTAGATGGCTTCGGTAAGGCGCTTATAATAGATAACT
TTATACAGAGCACGGAAGCCGATGAAGCATTATACCATGAACTACTCGTCCACCCAGCGATGGTGCTCCACCCGAAGCCA
TCTAGGGTTCTAATCATCGGTGGCGGCGAGGGGGCAACGCTCAGGGAGGTCCTAAAGCATAATACTGTTAAAGAAGCCGT
AATGGTGGATATTGATCCCGTGGTGGTAGAGTTCTCGAAGAAGTACCTTGAACACATGCACCAGGGGAGCTTCAATGATC
CAAGGTCAAGGGTGTTCATCATGGATGGATTGAAATACGTGGCTGAGGCACCCGACAAGTACTTTGATGTGGCAATAATG
GATCTCACGGATCCTTACGCAGGCGAGGCGGCGAAGCCCTTATACAGTGAGGGATTCTACAGGAGCGTTAAAAGAATACT
GGGGGATAACGGTGTTCTAGTCACACAGGCTGGAAGCAGCTACTTCTATACAAGCGAGTACGACTATGTGGCCGGTAATA
TTAGAAGGGTCTTCAAGTATATGGCGGAGTATAATATATGGATACCCAGCTTCGGGCTAAACGTCAACTTCATCATCGCA
TCAGATACCCTAGACCCGAGGAGTATTGCCCCAGGAGACTTCGATAAAAGACTCATGGAGAGAGGGGTGAAGACAAGGTT
CATTAATGGCGAGCGGTACAACGGTTTATTAATGTTCGGTGTATTGAAGCCTCTACACTGA

Upstream 100 bases:

>100_bases
GATGCCCTAGCATTAATAGCTGCAGGCGCATCTAGGATAGGTACTAGTTCAGGGGATAAGATAATCGAGGACTTTATAAG
GATTAAGGAGGGGAAGTAGG

Downstream 100 bases:

>100_bases
TTCTCTTTTTGAAATATACATGCTACGCCACTCCTTTTAACGCCGGGGGATCCGGCTGTGAGATATTGAGAGTCCTAGTT
TTAAAAGCACTGCTGTAAGA

Product: Spermine synthase

Products: NA

Alternate protein names: Putrescine aminopropyltransferase; PAPT; SPDSY [H]

Number of amino acids: Translated: 286; Mature: 285

Protein sequence:

>286_residues
MSGEEGLCLFEPAGRSFKLLLRVLKVYAVEKSPYQEIIFAELDGFGKALIIDNFIQSTEADEALYHELLVHPAMVLHPKP
SRVLIIGGGEGATLREVLKHNTVKEAVMVDIDPVVVEFSKKYLEHMHQGSFNDPRSRVFIMDGLKYVAEAPDKYFDVAIM
DLTDPYAGEAAKPLYSEGFYRSVKRILGDNGVLVTQAGSSYFYTSEYDYVAGNIRRVFKYMAEYNIWIPSFGLNVNFIIA
SDTLDPRSIAPGDFDKRLMERGVKTRFINGERYNGLLMFGVLKPLH

Sequences:

>Translated_286_residues
MSGEEGLCLFEPAGRSFKLLLRVLKVYAVEKSPYQEIIFAELDGFGKALIIDNFIQSTEADEALYHELLVHPAMVLHPKP
SRVLIIGGGEGATLREVLKHNTVKEAVMVDIDPVVVEFSKKYLEHMHQGSFNDPRSRVFIMDGLKYVAEAPDKYFDVAIM
DLTDPYAGEAAKPLYSEGFYRSVKRILGDNGVLVTQAGSSYFYTSEYDYVAGNIRRVFKYMAEYNIWIPSFGLNVNFIIA
SDTLDPRSIAPGDFDKRLMERGVKTRFINGERYNGLLMFGVLKPLH
>Mature_285_residues
SGEEGLCLFEPAGRSFKLLLRVLKVYAVEKSPYQEIIFAELDGFGKALIIDNFIQSTEADEALYHELLVHPAMVLHPKPS
RVLIIGGGEGATLREVLKHNTVKEAVMVDIDPVVVEFSKKYLEHMHQGSFNDPRSRVFIMDGLKYVAEAPDKYFDVAIMD
LTDPYAGEAAKPLYSEGFYRSVKRILGDNGVLVTQAGSSYFYTSEYDYVAGNIRRVFKYMAEYNIWIPSFGLNVNFIIAS
DTLDPRSIAPGDFDKRLMERGVKTRFINGERYNGLLMFGVLKPLH

Specific function: Catalyzes the production of spermidine from putrescine and decarboxylated S-adenosylmethionine (dcSAM), which serves as an aminopropyl donor [H]

COG id: COG0421

COG function: function code E; Spermidine synthase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the spermidine/spermine synthase family [H]

Homologues:

Organism=Homo sapiens, GI63253298, Length=224, Percent_Identity=32.1428571428571, Blast_Score=123, Evalue=2e-28,
Organism=Escherichia coli, GI1786312, Length=241, Percent_Identity=34.8547717842324, Blast_Score=155, Evalue=4e-39,
Organism=Caenorhabditis elegans, GI25154106, Length=263, Percent_Identity=31.1787072243346, Blast_Score=123, Evalue=9e-29,
Organism=Caenorhabditis elegans, GI212645616, Length=235, Percent_Identity=35.3191489361702, Blast_Score=121, Evalue=5e-28,
Organism=Saccharomyces cerevisiae, GI6325326, Length=225, Percent_Identity=36, Blast_Score=143, Evalue=3e-35,
Organism=Saccharomyces cerevisiae, GI6323175, Length=223, Percent_Identity=34.0807174887892, Blast_Score=134, Evalue=2e-32,
Organism=Drosophila melanogaster, GI24645443, Length=229, Percent_Identity=35.3711790393013, Blast_Score=132, Evalue=2e-31,
Organism=Drosophila melanogaster, GI21355941, Length=169, Percent_Identity=34.3195266272189, Blast_Score=96, Evalue=2e-20,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001045 [H]

Pfam domain/function: PF01564 Spermine_synth [H]

EC number: =2.5.1.16 [H]

Molecular weight: Translated: 32285; Mature: 32153

Theoretical pI: Translated: 5.93; Mature: 5.93

Prosite motif: PS01330 SPERMIDINE_SYNTHASE_1 ; PS51006 SPERMIDINE_SYNTHASE_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.3 %Cys     (Translated Protein)
3.1 %Met     (Translated Protein)
3.5 %Cys+Met (Translated Protein)
0.4 %Cys     (Mature Protein)
2.8 %Met     (Mature Protein)
3.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSGEEGLCLFEPAGRSFKLLLRVLKVYAVEKSPYQEIIFAELDGFGKALIIDNFIQSTEA
CCCCCCEEEECCCCCHHHHHHHHHHHHHCCCCCHHHHHHHHHCCCCCEEHHHHHHHCCCH
DEALYHELLVHPAMVLHPKPSRVLIIGGGEGATLREVLKHNTVKEAVMVDIDPVVVEFSK
HHHHHHHHHHCCCEEECCCCCEEEEEECCCCHHHHHHHHHCCHHHHEEEECCHHHHHHHH
KYLEHMHQGSFNDPRSRVFIMDGLKYVAEAPDKYFDVAIMDLTDPYAGEAAKPLYSEGFY
HHHHHHHCCCCCCCCCEEEEEHHHHHHHHCCCCCEEEEEEECCCCCCCCCCCCHHHHHHH
RSVKRILGDNGVLVTQAGSSYFYTSEYDYVAGNIRRVFKYMAEYNIWIPSFGLNVNFIIA
HHHHHHHCCCCEEEEECCCCEEEECCCCHHHHHHHHHHHHHHHCCEEEECCCCEEEEEEE
SDTLDPRSIAPGDFDKRLMERGVKTRFINGERYNGLLMFGVLKPLH
ECCCCCCCCCCCCHHHHHHHCCCCEEEECCCCCCCEEEEEECCCCC
>Mature Secondary Structure 
SGEEGLCLFEPAGRSFKLLLRVLKVYAVEKSPYQEIIFAELDGFGKALIIDNFIQSTEA
CCCCCEEEECCCCCHHHHHHHHHHHHHCCCCCHHHHHHHHHCCCCCEEHHHHHHHCCCH
DEALYHELLVHPAMVLHPKPSRVLIIGGGEGATLREVLKHNTVKEAVMVDIDPVVVEFSK
HHHHHHHHHHCCCEEECCCCCEEEEEECCCCHHHHHHHHHCCHHHHEEEECCHHHHHHHH
KYLEHMHQGSFNDPRSRVFIMDGLKYVAEAPDKYFDVAIMDLTDPYAGEAAKPLYSEGFY
HHHHHHHCCCCCCCCCEEEEEHHHHHHHHCCCCCEEEEEEECCCCCCCCCCCCHHHHHHH
RSVKRILGDNGVLVTQAGSSYFYTSEYDYVAGNIRRVFKYMAEYNIWIPSFGLNVNFIIA
HHHHHHHCCCCEEEEECCCCEEEECCCCHHHHHHHHHHHHHHHCCEEEECCCCEEEEEEE
SDTLDPRSIAPGDFDKRLMERGVKTRFINGERYNGLLMFGVLKPLH
ECCCCCCCCCCCCHHHHHHHCCCCEEEECCCCCCCEEEEEECCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA