Click here to switch to the map view.

The map label for this gene is dppA [H]

Identifier: 218883597

GI number: 218883597

Start: 265425

End: 267566

Strand: Direct

Name: dppA [H]

Synonym: DKAM_0286

Alternate gene names: 218883597

Gene position: 265425-267566 (Clockwise)

Preceding gene: 218883595

Following gene: 218883598

Centisome position: 19.44

GC content: 46.08

Gene sequence:

>2142_bases
GTGGTATATGTGAGTAAAGCTCTAAGTAGGACCGTTGCAGTAATCCTCATTCTAATAGTCGTAATAGCTATCGGCATAGG
AGCTTATTATTGGAGCAGCATGCAGCAGCCAACCCAGGTCACTAAGCCCACTAAGCCTATCCACACCCAGGTACTTTACT
TGATATCTAATGATGAGAATACCAGGATTCAATTATATCAATCAGGCACGGTTGATATAGCTGTTGTAACACCTAGCAGG
TGGAGGGATATAAATAATACGCCGGTAGGAAACTTTAAGCTTGTACTAGCCTTCGATCCAAGCAGGCCCATGTTAACAAT
ACAGCATGTAATCCTCAACACTATGAAGGAGCCGTTTAATATAACAGAGGTGAGGCAGGCCCTTGCATGGGCTACACCGT
ATCAGGCTATATTGGATCAAGTGTTCAGCGGGCTCTACACGAGGTTATATACAATAGTGCCTAAGGGGTTACAGGGATAC
ACCGAGTATAATATCGTTAAGTACGAGTATAATTTAACCAAGGCCAGGGAGATAATAGATCAATTAAAGCAGAGGGGCTT
CGATCCCTCCAAGTACACGATAGAAATAGCGTACAACCAGGGTAACACAGCGAGGCAGCAGATAGCTGCAATGCTGCAGA
ACGCGTGGAGCCAATTAGGCTTTAGAGTAATAGTTAACACATATAACTGGCCCCAATATCTCGATAAAGTAGATAACTTC
AACTTCGATGTTGCATTACTAGGCTGGATCCCAGACTACCTCGACCCAGATAACTACCTGATGCCATTCGTATGGGGTGG
TGCCGAGTTCACCGAGATCAATTACTATAAGGCTTCATCACCAAACGATGTATCCAACCACGTTTCCAGGGTTGAAAGGG
TTATAGACACGGAGAAATACATTGTTGTAGTAGGTCCGAAGGGTAGTGGGGCATCATATACTGGTCCAGCAAACAAGCCG
ATACTAGTTGTCAACTATGTGCTGGATGAGGAGGAAACCATGAAGAACTGGGAGAAACCAGTATCAATGGTGACTATAGG
TGCGCCTGGCTGGAAGGATATACCTGTAAGCGCGTTAGTGAAGCTCTCGAGAACAGTGCTTGACCCAGATATTAGGAGTG
CCGTAATCAATGCCGCCGTAATAGTGTTCAATAATGAGTCCCCGATGATAATGCTAGGGCAGCAGGTTGGAGAACGTAAT
TATGGATCATGGGTCTACAACATGTACTACCCGCTTTCAGCGTTCGCTAGATACGACTTGGTGTGGGAAGACCCTAATGC
ACCAGTGGTTGACACGGGTGTATCAGGGATAAGAAACGACCCTAACACTATGGTTATTGCTACTATTGGATGGCCGGATA
CATTTGACCCAGCTAAGTCCTACGAGTCGTTCGGATGGGAGATATTCTGGCAGATATACAGTAGACCAATAACATACTAC
TATGAAAACACTGATCCCGAGCCAGAGCTAGCCGTGGCATGGGCGTTCAGTAGTGACGCCACTGATCTATACCTTGTTAT
AAGGGGCGATGTACAGGCTTACGATCCATGGAACAATAAGACATACCCAATAGATGCAACCGACGTCCTATTCAGTATTT
GGAGAGTGGCTAGGACAAACCTGCCTGGAGGTCCATCCTGGATGATCTCGGACTTCATAGATGTTAATGCATCGCAGGTG
CTAACCGAGGACGAGTTTAAGCAGGTGGTAAGCAATGGCTTGTCAACCGTATATCATGGGCAGTCGAAGACCGTTAAAAG
CCTTAATGAACTACTAGGTTTCTTCAATTACTCTGGTCAGACGGCTGGGGTTGTCAAGTTAAAGCTGAAGTTCCCGTATC
CGCCTATACTCCACGTATTGACGACGGCTGTGGGATGTGTTATACCGATGGAGTATGCCCTAGGAAACCAATACCAGCAG
GCACTAGCCGACAGTAATAATGGTAAGAACCCCGCGGCGTGGGCCAGGTACGTTCTTGAAGGTGAAGACGACCCTACTTA
CAAGCTATTAAAGGATAAACCGGTGAGCACCGGTCCATACTACGTAGCCGACTACAAGGAAGACAGCTACATACTATTAA
AACTTAACCCATACTACTGGAACGCGACAGTATGGTATGAACTCTACGGCTTCAAACCATAG

Upstream 100 bases:

>100_bases
CATTCATTTCCCTCACTCAACACATTCCCCCAATAATGTATGGTATTAATAACGTGGGTAAAATTTATATTTTGTTTATA
CCTATTACTACACCATGTAG

Downstream 100 bases:

>100_bases
GTTACCTGATATTTTTTAAAGCCTCATCCTCGTTTCTACCCTATAAGAGGGAGATAAATGGGTCTGGGAAGATTTCTAGC
CAGAAGAATAGCTACCTTCA

Product: extracellular solute-binding protein, family 5

Products: ADP; phosphate; dipeptides [Cytoplasm] [C]

Alternate protein names: Dipeptide-binding protein; DBP [H]

Number of amino acids: Translated: 713; Mature: 713

Protein sequence:

>713_residues
MVYVSKALSRTVAVILILIVVIAIGIGAYYWSSMQQPTQVTKPTKPIHTQVLYLISNDENTRIQLYQSGTVDIAVVTPSR
WRDINNTPVGNFKLVLAFDPSRPMLTIQHVILNTMKEPFNITEVRQALAWATPYQAILDQVFSGLYTRLYTIVPKGLQGY
TEYNIVKYEYNLTKAREIIDQLKQRGFDPSKYTIEIAYNQGNTARQQIAAMLQNAWSQLGFRVIVNTYNWPQYLDKVDNF
NFDVALLGWIPDYLDPDNYLMPFVWGGAEFTEINYYKASSPNDVSNHVSRVERVIDTEKYIVVVGPKGSGASYTGPANKP
ILVVNYVLDEEETMKNWEKPVSMVTIGAPGWKDIPVSALVKLSRTVLDPDIRSAVINAAVIVFNNESPMIMLGQQVGERN
YGSWVYNMYYPLSAFARYDLVWEDPNAPVVDTGVSGIRNDPNTMVIATIGWPDTFDPAKSYESFGWEIFWQIYSRPITYY
YENTDPEPELAVAWAFSSDATDLYLVIRGDVQAYDPWNNKTYPIDATDVLFSIWRVARTNLPGGPSWMISDFIDVNASQV
LTEDEFKQVVSNGLSTVYHGQSKTVKSLNELLGFFNYSGQTAGVVKLKLKFPYPPILHVLTTAVGCVIPMEYALGNQYQQ
ALADSNNGKNPAAWARYVLEGEDDPTYKLLKDKPVSTGPYYVADYKEDSYILLKLNPYYWNATVWYELYGFKP

Sequences:

>Translated_713_residues
MVYVSKALSRTVAVILILIVVIAIGIGAYYWSSMQQPTQVTKPTKPIHTQVLYLISNDENTRIQLYQSGTVDIAVVTPSR
WRDINNTPVGNFKLVLAFDPSRPMLTIQHVILNTMKEPFNITEVRQALAWATPYQAILDQVFSGLYTRLYTIVPKGLQGY
TEYNIVKYEYNLTKAREIIDQLKQRGFDPSKYTIEIAYNQGNTARQQIAAMLQNAWSQLGFRVIVNTYNWPQYLDKVDNF
NFDVALLGWIPDYLDPDNYLMPFVWGGAEFTEINYYKASSPNDVSNHVSRVERVIDTEKYIVVVGPKGSGASYTGPANKP
ILVVNYVLDEEETMKNWEKPVSMVTIGAPGWKDIPVSALVKLSRTVLDPDIRSAVINAAVIVFNNESPMIMLGQQVGERN
YGSWVYNMYYPLSAFARYDLVWEDPNAPVVDTGVSGIRNDPNTMVIATIGWPDTFDPAKSYESFGWEIFWQIYSRPITYY
YENTDPEPELAVAWAFSSDATDLYLVIRGDVQAYDPWNNKTYPIDATDVLFSIWRVARTNLPGGPSWMISDFIDVNASQV
LTEDEFKQVVSNGLSTVYHGQSKTVKSLNELLGFFNYSGQTAGVVKLKLKFPYPPILHVLTTAVGCVIPMEYALGNQYQQ
ALADSNNGKNPAAWARYVLEGEDDPTYKLLKDKPVSTGPYYVADYKEDSYILLKLNPYYWNATVWYELYGFKP
>Mature_713_residues
MVYVSKALSRTVAVILILIVVIAIGIGAYYWSSMQQPTQVTKPTKPIHTQVLYLISNDENTRIQLYQSGTVDIAVVTPSR
WRDINNTPVGNFKLVLAFDPSRPMLTIQHVILNTMKEPFNITEVRQALAWATPYQAILDQVFSGLYTRLYTIVPKGLQGY
TEYNIVKYEYNLTKAREIIDQLKQRGFDPSKYTIEIAYNQGNTARQQIAAMLQNAWSQLGFRVIVNTYNWPQYLDKVDNF
NFDVALLGWIPDYLDPDNYLMPFVWGGAEFTEINYYKASSPNDVSNHVSRVERVIDTEKYIVVVGPKGSGASYTGPANKP
ILVVNYVLDEEETMKNWEKPVSMVTIGAPGWKDIPVSALVKLSRTVLDPDIRSAVINAAVIVFNNESPMIMLGQQVGERN
YGSWVYNMYYPLSAFARYDLVWEDPNAPVVDTGVSGIRNDPNTMVIATIGWPDTFDPAKSYESFGWEIFWQIYSRPITYY
YENTDPEPELAVAWAFSSDATDLYLVIRGDVQAYDPWNNKTYPIDATDVLFSIWRVARTNLPGGPSWMISDFIDVNASQV
LTEDEFKQVVSNGLSTVYHGQSKTVKSLNELLGFFNYSGQTAGVVKLKLKFPYPPILHVLTTAVGCVIPMEYALGNQYQQ
ALADSNNGKNPAAWARYVLEGEDDPTYKLLKDKPVSTGPYYVADYKEDSYILLKLNPYYWNATVWYELYGFKP

Specific function: Dipeptide-binding protein of a transport system that can be subject to osmotic shock. DppA is also required for peptide chemotaxis [H]

COG id: COG0747

COG function: function code E; ABC-type dipeptide transport system, periplasmic component

Gene ontology:

Cell location: Periplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the bacterial solute-binding protein 5 family [H]

Homologues:

Organism=Escherichia coli, GI1789966, Length=176, Percent_Identity=26.7045454545455, Blast_Score=82, Evalue=2e-16,
Organism=Escherichia coli, GI1787762, Length=231, Percent_Identity=27.7056277056277, Blast_Score=77, Evalue=3e-15,
Organism=Escherichia coli, GI1789397, Length=214, Percent_Identity=26.6355140186916, Blast_Score=65, Evalue=2e-11,

Paralogues:

None

Copy number: 660 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 2980 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 40 Molecules/Cell In: Stationary Phase, Rich Media (Based on E. coli). [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000914 [H]

Pfam domain/function: PF00496 SBP_bac_5 [H]

EC number: NA

Molecular weight: Translated: 80845; Mature: 80845

Theoretical pI: Translated: 4.78; Mature: 4.78

Prosite motif: PS00018 EF_HAND_1

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.1 %Cys     (Translated Protein)
2.0 %Met     (Translated Protein)
2.1 %Cys+Met (Translated Protein)
0.1 %Cys     (Mature Protein)
2.0 %Met     (Mature Protein)
2.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MVYVSKALSRTVAVILILIVVIAIGIGAYYWSSMQQPTQVTKPTKPIHTQVLYLISNDEN
CEEHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCEEEEEEEEECCCC
TRIQLYQSGTVDIAVVTPSRWRDINNTPVGNFKLVLAFDPSRPMLTIQHVILNTMKEPFN
CEEEEEECCCEEEEEECCHHCCCCCCCCCCCEEEEEEECCCCCCHHHHHHHHHHHCCCCC
ITEVRQALAWATPYQAILDQVFSGLYTRLYTIVPKGLQGYTEYNIVKYEYNLTKAREIID
HHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCEEEEEEEECHHHHHHHHH
QLKQRGFDPSKYTIEIAYNQGNTARQQIAAMLQNAWSQLGFRVIVNTYNWPQYLDKVDNF
HHHHCCCCCCCEEEEEEECCCCHHHHHHHHHHHHHHHHCCEEEEEECCCCHHHHHHHCCC
NFDVALLGWIPDYLDPDNYLMPFVWGGAEFTEINYYKASSPNDVSNHVSRVERVIDTEKY
CCCEEEEECCCCCCCCCCCEEEEEECCCEEEEEEEEECCCCCHHHHHHHHHHHHHCCCCE
IVVVGPKGSGASYTGPANKPILVVNYVLDEEETMKNWEKPVSMVTIGAPGWKDIPVSALV
EEEECCCCCCCCCCCCCCCCEEEEEEEECCHHHHHHHCCCEEEEEECCCCCCCCCHHHHH
KLSRTVLDPDIRSAVINAAVIVFNNESPMIMLGQQVGERNYGSWVYNMYYPLSAFARYDL
HHHHHHCCHHHHHHHEEEEEEEECCCCCEEEEEHHHCCCCCCCEEEEEECCHHHHHEEEE
VWEDPNAPVVDTGVSGIRNDPNTMVIATIGWPDTFDPAKSYESFGWEIFWQIYSRPITYY
EEECCCCCEEECCCCCCCCCCCEEEEEEECCCCCCCHHHHHHHCCHHEEEEHHCCCEEEE
YENTDPEPELAVAWAFSSDATDLYLVIRGDVQAYDPWNNKTYPIDATDVLFSIWRVARTN
ECCCCCCCCEEEEEEECCCCCEEEEEEECCEEEECCCCCCCCCCCHHHHHHHHHHHHHCC
LPGGPSWMISDFIDVNASQVLTEDEFKQVVSNGLSTVYHGQSKTVKSLNELLGFFNYSGQ
CCCCCCHHHHHHHCCCHHHHCCHHHHHHHHHCCCCEEECCCCHHHHHHHHHHHHHCCCCC
TAGVVKLKLKFPYPPILHVLTTAVGCVIPMEYALGNQYQQALADSNNGKNPAAWARYVLE
CCEEEEEEEECCCCHHHHHHHHHHHHHEEHHHHHCHHHHHHHHCCCCCCCHHHHHHEEEE
GEDDPTYKLLKDKPVSTGPYYVADYKEDSYILLKLNPYYWNATVWYELYGFKP
CCCCCCEEEECCCCCCCCCEEEEECCCCCEEEEEECCEEECEEEEEEEECCCC
>Mature Secondary Structure
MVYVSKALSRTVAVILILIVVIAIGIGAYYWSSMQQPTQVTKPTKPIHTQVLYLISNDEN
CEEHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCEEEEEEEEECCCC
TRIQLYQSGTVDIAVVTPSRWRDINNTPVGNFKLVLAFDPSRPMLTIQHVILNTMKEPFN
CEEEEEECCCEEEEEECCHHCCCCCCCCCCCEEEEEEECCCCCCHHHHHHHHHHHCCCCC
ITEVRQALAWATPYQAILDQVFSGLYTRLYTIVPKGLQGYTEYNIVKYEYNLTKAREIID
HHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCEEEEEEEECHHHHHHHHH
QLKQRGFDPSKYTIEIAYNQGNTARQQIAAMLQNAWSQLGFRVIVNTYNWPQYLDKVDNF
HHHHCCCCCCCEEEEEEECCCCHHHHHHHHHHHHHHHHCCEEEEEECCCCHHHHHHHCCC
NFDVALLGWIPDYLDPDNYLMPFVWGGAEFTEINYYKASSPNDVSNHVSRVERVIDTEKY
CCCEEEEECCCCCCCCCCCEEEEEECCCEEEEEEEEECCCCCHHHHHHHHHHHHHCCCCE
IVVVGPKGSGASYTGPANKPILVVNYVLDEEETMKNWEKPVSMVTIGAPGWKDIPVSALV
EEEECCCCCCCCCCCCCCCCEEEEEEEECCHHHHHHHCCCEEEEEECCCCCCCCCHHHHH
KLSRTVLDPDIRSAVINAAVIVFNNESPMIMLGQQVGERNYGSWVYNMYYPLSAFARYDL
HHHHHHCCHHHHHHHEEEEEEEECCCCCEEEEEHHHCCCCCCCEEEEEECCHHHHHEEEE
VWEDPNAPVVDTGVSGIRNDPNTMVIATIGWPDTFDPAKSYESFGWEIFWQIYSRPITYY
EEECCCCCEEECCCCCCCCCCCEEEEEEECCCCCCCHHHHHHHCCHHEEEEHHCCCEEEE
YENTDPEPELAVAWAFSSDATDLYLVIRGDVQAYDPWNNKTYPIDATDVLFSIWRVARTN
ECCCCCCCCEEEEEEECCCCCEEEEEEECCEEEECCCCCCCCCCCHHHHHHHHHHHHHCC
LPGGPSWMISDFIDVNASQVLTEDEFKQVVSNGLSTVYHGQSKTVKSLNELLGFFNYSGQ
CCCCCCHHHHHHHCCCHHHHCCHHHHHHHHHCCCCEEECCCCHHHHHHHHHHHHHCCCCC
TAGVVKLKLKFPYPPILHVLTTAVGCVIPMEYALGNQYQQALADSNNGKNPAAWARYVLE
CCEEEEEEEECCCCHHHHHHHHHHHHHEEHHHHHCHHHHHHHHCCCCCCCHHHHHHEEEE
GEDDPTYKLLKDKPVSTGPYYVADYKEDSYILLKLNPYYWNATVWYELYGFKP
CCCCCCEEEECCCCCCCCCEEEEECCCCCEEEEEECCEEECEEEEEEEECCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: ATP; dipeptides [Periplasm]; H2O [C]

Specific reaction: ATP + dipeptides [Periplasm] + H2O = ADP + phosphate + dipeptides [Cytoplasm] [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 1702779; 1956284; 7536291; 8041620; 9278503; 9298646; 9600841; 8563629; 8527431 [H]