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The map label for this gene is gph [C]

Identifier: 218883514

GI number: 218883514

Start: 190081

End: 190809

Strand: Reverse

Name: gph [C]

Synonym: DKAM_0201

Alternate gene names: 218883514

Gene position: 190809-190081 (Counterclockwise)

Preceding gene: 218883515

Following gene: 218883513

Centisome position: 13.98

GC content: 45.68

Gene sequence:

>729_bases
ATGGCTGGTTGCCTGGCTATAAGTTTCGATATATGGGGTACGCTTGTGGATCTCGATAAAACCCTTGATCACCTCTCAGA
GATAGCTTCTAATAGGCTGGGTTTAAGCATCGATGAAGCGCGGAAAGCAATATACGTATCGCATGAGGAGGCCCGGAAGC
TCAGGAGGTTCACGCCGAATATTCCACCACTTGAGCTCATTGCACGTGGCAAAGAGATAATCGCGGCAAAGCTCTCAACC
AGCCTCAACACTGTTGACTCCATCCTAATGGAAGCGTTCACAACCGTTGAACCGGGATCGATAATCTACCCTGATGTTAT
ACCGGTTTTAAGTAAGCTCCATGATGAAGGCATCTATATGGGTGTAGTGGGCAATGTTCTATTCTGGCCCAGTGTTTACA
CAATGATCATATTGGATAAAACCGGGCTCTCAAAATACTTTAGGGTAGCCGTATTCTCGGACATTATAGGCTATAGCAAA
CCAGATAGGGAGATATTCCTGGAATTCGCTAAGATCTCTGGTTTCGAGCCAAACAGGGTAATACATGTAGGAGACAATGT
TATCGAGGATGTTGGTGGAGCATTGTCATCGGGATTCCTGGGTGTATTGATAAATAGGAGGTCTAATAGGAGTATATATG
TATGGGAGCTCAACGCGGCCGTAATTAACGACATGAGGAACCTAGTAGACCTCTACAGGGAGGCATCGCTGAAGATATGC
CGTAAATAG

Upstream 100 bases:

>100_bases
CCCCAGCGCGCTCGAGATAGGTTTAAAGATACTAATGGATATTATTGAACACCTCGTTGAAGAAGTGGAGAGATTTGAGG
GTCCGCATAGGTGATTAGGT

Downstream 100 bases:

>100_bases
TGTATTGATTTACTGGTGATCGAGAAAATGATGAGGATAGAGGAAAACAGGGAGAGAATACTTTACGGGTCCATTGGTAG
AACACTCCTATGGCTTGGGC

Product: putative hydrolase

Products: Glycolate; Phosphate [C]

Alternate protein names: NA

Number of amino acids: Translated: 242; Mature: 241

Protein sequence:

>242_residues
MAGCLAISFDIWGTLVDLDKTLDHLSEIASNRLGLSIDEARKAIYVSHEEARKLRRFTPNIPPLELIARGKEIIAAKLST
SLNTVDSILMEAFTTVEPGSIIYPDVIPVLSKLHDEGIYMGVVGNVLFWPSVYTMIILDKTGLSKYFRVAVFSDIIGYSK
PDREIFLEFAKISGFEPNRVIHVGDNVIEDVGGALSSGFLGVLINRRSNRSIYVWELNAAVINDMRNLVDLYREASLKIC
RK

Sequences:

>Translated_242_residues
MAGCLAISFDIWGTLVDLDKTLDHLSEIASNRLGLSIDEARKAIYVSHEEARKLRRFTPNIPPLELIARGKEIIAAKLST
SLNTVDSILMEAFTTVEPGSIIYPDVIPVLSKLHDEGIYMGVVGNVLFWPSVYTMIILDKTGLSKYFRVAVFSDIIGYSK
PDREIFLEFAKISGFEPNRVIHVGDNVIEDVGGALSSGFLGVLINRRSNRSIYVWELNAAVINDMRNLVDLYREASLKIC
RK
>Mature_241_residues
AGCLAISFDIWGTLVDLDKTLDHLSEIASNRLGLSIDEARKAIYVSHEEARKLRRFTPNIPPLELIARGKEIIAAKLSTS
LNTVDSILMEAFTTVEPGSIIYPDVIPVLSKLHDEGIYMGVVGNVLFWPSVYTMIILDKTGLSKYFRVAVFSDIIGYSKP
DREIFLEFAKISGFEPNRVIHVGDNVIEDVGGALSSGFLGVLINRRSNRSIYVWELNAAVINDMRNLVDLYREASLKICR
K

Specific function: Unknown

COG id: COG1011

COG function: function code R; Predicted hydrolase (HAD superfamily)

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the HAD-like hydrolase superfamily [H]

Homologues:

None

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR005834
- InterPro:   IPR023214
- InterPro:   IPR011950
- InterPro:   IPR006439
- InterPro:   IPR006402
- InterPro:   IPR005833 [H]

Pfam domain/function: PF00702 Hydrolase [H]

EC number: 3.1.3.18 [C]

Molecular weight: Translated: 27007; Mature: 26875

Theoretical pI: Translated: 6.14; Mature: 6.14

Prosite motif: PS00761 SPASE_I_3

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.8 %Cys     (Translated Protein)
2.1 %Met     (Translated Protein)
2.9 %Cys+Met (Translated Protein)
0.8 %Cys     (Mature Protein)
1.7 %Met     (Mature Protein)
2.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MAGCLAISFDIWGTLVDLDKTLDHLSEIASNRLGLSIDEARKAIYVSHEEARKLRRFTPN
CCCEEEEEHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHEEECHHHHHHHHHHCCC
IPPLELIARGKEIIAAKLSTSLNTVDSILMEAFTTVEPGSIIYPDVIPVLSKLHDEGIYM
CCHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCEECCHHHHHHHHHHHCCEEE
GVVGNVLFWPSVYTMIILDKTGLSKYFRVAVFSDIIGYSKPDREIFLEFAKISGFEPNRV
EHHHHHHHHHHHEEEEEEECCCHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHCCCCCCCE
IHVGDNVIEDVGGALSSGFLGVLINRRSNRSIYVWELNAAVINDMRNLVDLYREASLKIC
EECCCHHHHHHHHHHHCCCEEEEEECCCCCEEEEEECHHHHHHHHHHHHHHHHHHHHHHC
RK
CC
>Mature Secondary Structure 
AGCLAISFDIWGTLVDLDKTLDHLSEIASNRLGLSIDEARKAIYVSHEEARKLRRFTPN
CCEEEEEHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHEEECHHHHHHHHHHCCC
IPPLELIARGKEIIAAKLSTSLNTVDSILMEAFTTVEPGSIIYPDVIPVLSKLHDEGIYM
CCHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCEECCHHHHHHHHHHHCCEEE
GVVGNVLFWPSVYTMIILDKTGLSKYFRVAVFSDIIGYSKPDREIFLEFAKISGFEPNRV
EHHHHHHHHHHHEEEEEEECCCHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHCCCCCCCE
IHVGDNVIEDVGGALSSGFLGVLINRRSNRSIYVWELNAAVINDMRNLVDLYREASLKIC
EECCCHHHHHHHHHHHCCCEEEEEECCCCCEEEEEECHHHHHHHHHHHHHHHHHHHHHHC
RK
CC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: Mg2+ [C]

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): 0.57 {phosphoglycolate}} [C]

Substrates: D-Glycerate 2-phosphatelyc; H2O [C]

Specific reaction: D-Glycerate 2-phosphatelyc + H2O --> Glycolate + Phosphate [C]

General reaction: Additional information:

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 11930014 [H]