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The map label for this gene is truC [H]

Identifier: 218710322

GI number: 218710322

Start: 2527324

End: 2528082

Strand: Reverse

Name: truC [H]

Synonym: VS_2357

Alternate gene names: 218710322

Gene position: 2528082-2527324 (Counterclockwise)

Preceding gene: 218710323

Following gene: 218710318

Centisome position: 76.62

GC content: 46.51

Gene sequence:

>759_bases
ATGCGCGTAATGTTAGAGATCATTTATCAAGATGAGTATTTTGTCGCGGTGAATAAGCCTGCGGGTATGCTAGTGCATCG
CTCATGGTTGGATAAACACGAGACTCAGTTTGTGATGCAGACTCTGCGCGACCAAATTGGTCAGCACGTATTTCCTCTGC
ATCGCTTAGACCGTCCAACGTCTGGCGTATTGGTGTTTGCTCTGTCGAGTGAAGTTGCCTCTGAGGTGATGCCGATGTTC
GCCAACCACGAGATGCAAAAAACCTATCATGCGATTGTGCGTGGTTGGATAGAAGAGGGCGATACGCTCGATTATGCGCT
TAAGGTTGAGCTGGATAAGATCGCCGATAAGTTCGCGAAAGAAGATAAAGAACCACAAGAAGCCGTCACTGTTTACGAGC
CGTTAGCGAAAGTCGAAGTGCCGTATTCAACAGGCCGTTTTCCTACCAGTCGTTATTGCTTAGTTGAGATGATGCCAAAG
ACAGGACGTAAACATCAGCTGCGTCGTCACATGGCTCATCTAAGACACCCAATCGTGGGCGATACTTCACATGGTGATGG
TAAGCATAACCGACTGTTCCGTGATGATTTAGACTCACACCGTCTGTTGCTGCATGCTTCTGAATTGAGCTTCATTCATC
CTTTCACTAAAGAAGAATTGGTGATGAAGGCGAACTTAGATGAAACGTGGTTAAGGTTGTTTGAAACTTTTGAGTGGGAT
GCCAACTTAATCGGTGCTAAGGCGTGTTCGTCTAAGTAA

Upstream 100 bases:

>100_bases
TACCGAAAGGCTTTGCGATTCATCCTTATTTTGGTGAAGTGTGGAAAAATGAGGCAGACAAAGCTGAACTGCTAGTGACG
ATTCAGAGCATTGATGAGGT

Downstream 100 bases:

>100_bases
AATACAAACGTCTAATTAAGCGTAGAACGTAAAGTAAAACAAAGGGCTGATAGTGATATCAGCCCTTTTTGACGTCTGTG
CGACCTAAACAACATAACTA

Product: tRNA pseudouridine synthase C

Products: NA

Alternate protein names: tRNA pseudouridylate synthase C; tRNA-uridine isomerase C [H]

Number of amino acids: Translated: 252; Mature: 252

Protein sequence:

>252_residues
MRVMLEIIYQDEYFVAVNKPAGMLVHRSWLDKHETQFVMQTLRDQIGQHVFPLHRLDRPTSGVLVFALSSEVASEVMPMF
ANHEMQKTYHAIVRGWIEEGDTLDYALKVELDKIADKFAKEDKEPQEAVTVYEPLAKVEVPYSTGRFPTSRYCLVEMMPK
TGRKHQLRRHMAHLRHPIVGDTSHGDGKHNRLFRDDLDSHRLLLHASELSFIHPFTKEELVMKANLDETWLRLFETFEWD
ANLIGAKACSSK

Sequences:

>Translated_252_residues
MRVMLEIIYQDEYFVAVNKPAGMLVHRSWLDKHETQFVMQTLRDQIGQHVFPLHRLDRPTSGVLVFALSSEVASEVMPMF
ANHEMQKTYHAIVRGWIEEGDTLDYALKVELDKIADKFAKEDKEPQEAVTVYEPLAKVEVPYSTGRFPTSRYCLVEMMPK
TGRKHQLRRHMAHLRHPIVGDTSHGDGKHNRLFRDDLDSHRLLLHASELSFIHPFTKEELVMKANLDETWLRLFETFEWD
ANLIGAKACSSK
>Mature_252_residues
MRVMLEIIYQDEYFVAVNKPAGMLVHRSWLDKHETQFVMQTLRDQIGQHVFPLHRLDRPTSGVLVFALSSEVASEVMPMF
ANHEMQKTYHAIVRGWIEEGDTLDYALKVELDKIADKFAKEDKEPQEAVTVYEPLAKVEVPYSTGRFPTSRYCLVEMMPK
TGRKHQLRRHMAHLRHPIVGDTSHGDGKHNRLFRDDLDSHRLLLHASELSFIHPFTKEELVMKANLDETWLRLFETFEWD
ANLIGAKACSSK

Specific function: Responsible for synthesis of pseudouridine from uracil- 65 in transfer RNAs [H]

COG id: COG0564

COG function: function code J; Pseudouridylate synthases, 23S RNA-specific

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the pseudouridine synthase rluA family [H]

Homologues:

Organism=Homo sapiens, GI221316667, Length=198, Percent_Identity=32.3232323232323, Blast_Score=69, Evalue=3e-12,
Organism=Homo sapiens, GI23308689, Length=179, Percent_Identity=28.4916201117318, Blast_Score=67, Evalue=1e-11,
Organism=Escherichia coli, GI1789155, Length=240, Percent_Identity=62.9166666666667, Blast_Score=315, Evalue=3e-87,
Organism=Escherichia coli, GI1787327, Length=233, Percent_Identity=31.3304721030043, Blast_Score=96, Evalue=2e-21,
Organism=Escherichia coli, GI1786244, Length=223, Percent_Identity=32.7354260089686, Blast_Score=96, Evalue=3e-21,
Organism=Escherichia coli, GI1788946, Length=254, Percent_Identity=25.9842519685039, Blast_Score=79, Evalue=4e-16,
Organism=Saccharomyces cerevisiae, GI6321608, Length=197, Percent_Identity=28.9340101522843, Blast_Score=77, Evalue=2e-15,
Organism=Saccharomyces cerevisiae, GI6324506, Length=243, Percent_Identity=25.9259259259259, Blast_Score=71, Evalue=1e-13,
Organism=Saccharomyces cerevisiae, GI6320168, Length=180, Percent_Identity=26.6666666666667, Blast_Score=71, Evalue=2e-13,
Organism=Drosophila melanogaster, GI24583449, Length=184, Percent_Identity=27.7173913043478, Blast_Score=71, Evalue=7e-13,
Organism=Drosophila melanogaster, GI24583447, Length=184, Percent_Identity=27.7173913043478, Blast_Score=71, Evalue=7e-13,
Organism=Drosophila melanogaster, GI24583445, Length=184, Percent_Identity=27.7173913043478, Blast_Score=71, Evalue=7e-13,
Organism=Drosophila melanogaster, GI19921090, Length=177, Percent_Identity=28.2485875706215, Blast_Score=70, Evalue=2e-12,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR020103
- InterPro:   IPR006224
- InterPro:   IPR006145 [H]

Pfam domain/function: PF00849 PseudoU_synth_2 [H]

EC number: NA

Molecular weight: Translated: 29271; Mature: 29271

Theoretical pI: Translated: 6.80; Mature: 6.80

Prosite motif: PS01129 PSI_RLU

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.8 %Cys     (Translated Protein)
4.4 %Met     (Translated Protein)
5.2 %Cys+Met (Translated Protein)
0.8 %Cys     (Mature Protein)
4.4 %Met     (Mature Protein)
5.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MRVMLEIIYQDEYFVAVNKPAGMLVHRSWLDKHETQFVMQTLRDQIGQHVFPLHRLDRPT
CCEEEEEEECCCEEEEECCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHCCCCC
SGVLVFALSSEVASEVMPMFANHEMQKTYHAIVRGWIEEGDTLDYALKVELDKIADKFAK
CCEEEEEECHHHHHHHHHHHHCHHHHHHHHHHHHHHHHCCCCEEEEEEEEHHHHHHHHHH
EDKEPQEAVTVYEPLAKVEVPYSTGRFPTSRYCLVEMMPKTGRKHQLRRHMAHLRHPIVG
CCCCHHHHHHHHHHHHHEECCCCCCCCCCCCCCEEEECCCCCHHHHHHHHHHHHCCCCCC
DTSHGDGKHNRLFRDDLDSHRLLLHASELSFIHPFTKEELVMKANLDETWLRLFETFEWD
CCCCCCCCCCCCHHHCCCCCEEEEEECCHHHCCCCCHHHEEEECCCCHHHHHHHHHHCCC
ANLIGAKACSSK
CCCCCCCCCCCC
>Mature Secondary Structure
MRVMLEIIYQDEYFVAVNKPAGMLVHRSWLDKHETQFVMQTLRDQIGQHVFPLHRLDRPT
CCEEEEEEECCCEEEEECCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHCCCCC
SGVLVFALSSEVASEVMPMFANHEMQKTYHAIVRGWIEEGDTLDYALKVELDKIADKFAK
CCEEEEEECHHHHHHHHHHHHCHHHHHHHHHHHHHHHHCCCCEEEEEEEEHHHHHHHHHH
EDKEPQEAVTVYEPLAKVEVPYSTGRFPTSRYCLVEMMPKTGRKHQLRRHMAHLRHPIVG
CCCCHHHHHHHHHHHHHEECCCCCCCCCCCCCCEEEECCCCCHHHHHHHHHHHHCCCCCC
DTSHGDGKHNRLFRDDLDSHRLLLHASELSFIHPFTKEELVMKANLDETWLRLFETFEWD
CCCCCCCCCCCCHHHCCCCCEEEEEECCHHHCCCCCHHHEEEECCCCHHHHHHHHHHCCC
ANLIGAKACSSK
CCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 12620739 [H]