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The map label for this gene is hsdM [H]

Identifier: 218709368

GI number: 218709368

Start: 1489378

End: 1490832

Strand: Direct

Name: hsdM [H]

Synonym: VS_1377

Alternate gene names: 218709368

Gene position: 1489378-1490832 (Clockwise)

Preceding gene: 218709367

Following gene: 218709369

Centisome position: 45.14

GC content: 47.7

Gene sequence:

>1455_bases
ATGAACAATAATGATTTGGTCGCCAAACTGTGGAAACTGTGTGACAACCTACGCGATGGTGGTGTGTCTTACCAAAACTA
CGTAAATGAATTGGCGTCGCTGCTGTTTCTCAAAATGTGTGAAGAAACAGGTCAAGAAGATGATTTGCTGCCAGAGGGCT
ATCGCTGGGCCGACCTTAAGGCAAAGCTAGGTCAAGAGCAGCACCAGTTCTACCGTAACATGCTGGTTCAGCTTGGTGCT
GACGACCATGCCATTGTTCGTGCTATCTTCCAAAACGTAAACACCACCATTACTCAACCTGCTCAATTGACCGAGTTGGT
GGACAACATGGACAAACTAGAATGGTTCGATGGCGATACTGGTAAGAGCCGTGATGACTTTGGTGACATGTACGAGGGTC
TACTGCAAAAGAACGCGAACGAGACAAAATCGGGCGCGGGTCAGTACTTTACGCCGCGTTCACTGATCAGCACCATCATC
AAAGTAATGCAGCCTCAGCCACGTGAAGTGATTCAAGATCCAGCAGCGGGTACGGCAGGTTTCTTGATCGAGGCCGATAA
ATACATCAAATCACGAACCAACGATTTAGATGATCTAGACGATGATGATCAAGAGTTCCAAATGACCAAAGCCTTTGTCG
GTTTGGAGCTAGTACCAGAAACACGCCGTTTAGCATTGATGAACTGCCTACTGCACGACATTGAAGGCGATGCAGAAGAG
GGCGCTATTCGCCTTGGCAACACGCTAGGCAGTGCAGGTGAAACCCTTCCTCAAGCAAACGTGATTCTGACTAACCCTCC
GTTTGGCAGCGCATCAAGTACCAACATCACTCGTACCTTTGTTCACCCAACAGGTAACAAACAGCTCTGTTTTATGCAGC
ATATCTATGATGCACTAGAGCCAGGCGGCCGTGCAGCGGTGGTAATTCCTGATAACGTGTTATTTGAAGGCGGCAAGGGA
GCGGACATTCGCCGTGACCTAATGGATAAGTGTAATTTGCACACTATTCTGCGTTTGCCTACTGGCATCTTCTACGCCGC
AGGGGTAAAGACTAATGTCCTTTTCTTCCAAAAAGGTACGCAACAGGATCCGAACCAAGACAAAGGCTGTACCAAAGAAA
CTTGGGTATTCGATATGCGTACCAACATGAATACTTTTGGTAAACGCCGCCCACTGACTGAAAAGCACTTTGATGTGTTT
GTGAATGCGTATGGCAGCGATACAAACGGTTTGTCTGCTCGTGAAGAAGGTGTTTACGAGACGTTAGGCAACATCTTTGC
TGAGGGTGATGATTCGGTAGAAAACCTAATCGAGAACGCTCGTTTCCGCAAATTCAGCCGTGACTACATTCGCGATCAAA
AAGGCGATAGCTTAGATATTTCATGGCTTAAAGACTTAGAAGCCACCAGCGCAGAAAACCTACCAGAGCCCAGAGCCAGA
AGTGCTAGCCGGTGA

Upstream 100 bases:

>100_bases
ATTCCAGAAGTGAGGTACGAACTATCTGGAATCTCTGATTAGCACTTGTCTTAAACAAATTACCTATCTGGCACGAGTCA
GAACAAAAAAGAGAACAAGC

Downstream 100 bases:

>100_bases
AGCGATGGCAGAGCTGACCGAAGCCATGTCTGAAATTTACCAACTGATGCAAGCACTCGGTGCGAATGACGAAGCAGAAG
GGCAAAAGCAGTTACTTGAG

Product: type I restriction enzyme EcoKI M subunit

Products: NA

Alternate protein names: M.EcoKI [H]

Number of amino acids: Translated: 484; Mature: 484

Protein sequence:

>484_residues
MNNNDLVAKLWKLCDNLRDGGVSYQNYVNELASLLFLKMCEETGQEDDLLPEGYRWADLKAKLGQEQHQFYRNMLVQLGA
DDHAIVRAIFQNVNTTITQPAQLTELVDNMDKLEWFDGDTGKSRDDFGDMYEGLLQKNANETKSGAGQYFTPRSLISTII
KVMQPQPREVIQDPAAGTAGFLIEADKYIKSRTNDLDDLDDDDQEFQMTKAFVGLELVPETRRLALMNCLLHDIEGDAEE
GAIRLGNTLGSAGETLPQANVILTNPPFGSASSTNITRTFVHPTGNKQLCFMQHIYDALEPGGRAAVVIPDNVLFEGGKG
ADIRRDLMDKCNLHTILRLPTGIFYAAGVKTNVLFFQKGTQQDPNQDKGCTKETWVFDMRTNMNTFGKRRPLTEKHFDVF
VNAYGSDTNGLSAREEGVYETLGNIFAEGDDSVENLIENARFRKFSRDYIRDQKGDSLDISWLKDLEATSAENLPEPRAR
SASR

Sequences:

>Translated_484_residues
MNNNDLVAKLWKLCDNLRDGGVSYQNYVNELASLLFLKMCEETGQEDDLLPEGYRWADLKAKLGQEQHQFYRNMLVQLGA
DDHAIVRAIFQNVNTTITQPAQLTELVDNMDKLEWFDGDTGKSRDDFGDMYEGLLQKNANETKSGAGQYFTPRSLISTII
KVMQPQPREVIQDPAAGTAGFLIEADKYIKSRTNDLDDLDDDDQEFQMTKAFVGLELVPETRRLALMNCLLHDIEGDAEE
GAIRLGNTLGSAGETLPQANVILTNPPFGSASSTNITRTFVHPTGNKQLCFMQHIYDALEPGGRAAVVIPDNVLFEGGKG
ADIRRDLMDKCNLHTILRLPTGIFYAAGVKTNVLFFQKGTQQDPNQDKGCTKETWVFDMRTNMNTFGKRRPLTEKHFDVF
VNAYGSDTNGLSAREEGVYETLGNIFAEGDDSVENLIENARFRKFSRDYIRDQKGDSLDISWLKDLEATSAENLPEPRAR
SASR
>Mature_484_residues
MNNNDLVAKLWKLCDNLRDGGVSYQNYVNELASLLFLKMCEETGQEDDLLPEGYRWADLKAKLGQEQHQFYRNMLVQLGA
DDHAIVRAIFQNVNTTITQPAQLTELVDNMDKLEWFDGDTGKSRDDFGDMYEGLLQKNANETKSGAGQYFTPRSLISTII
KVMQPQPREVIQDPAAGTAGFLIEADKYIKSRTNDLDDLDDDDQEFQMTKAFVGLELVPETRRLALMNCLLHDIEGDAEE
GAIRLGNTLGSAGETLPQANVILTNPPFGSASSTNITRTFVHPTGNKQLCFMQHIYDALEPGGRAAVVIPDNVLFEGGKG
ADIRRDLMDKCNLHTILRLPTGIFYAAGVKTNVLFFQKGTQQDPNQDKGCTKETWVFDMRTNMNTFGKRRPLTEKHFDVF
VNAYGSDTNGLSAREEGVYETLGNIFAEGDDSVENLIENARFRKFSRDYIRDQKGDSLDISWLKDLEATSAENLPEPRAR
SASR

Specific function: The M and S subunits together form a methyltransferase (MTase) that methylates two adenine residues in complementary strands of a bipartite DNA recognition sequence. In the presence of the R subunit the complex can also act as an endonuclease, binding to

COG id: COG0286

COG function: function code V; Type I restriction-modification system methyltransferase subunit

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the N(4)/N(6)-methyltransferase family [H]

Homologues:

Organism=Escherichia coli, GI1790808, Length=488, Percent_Identity=71.7213114754098, Blast_Score=733, Evalue=0.0,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR022749
- InterPro:   IPR003356
- InterPro:   IPR002052
- InterPro:   IPR002296 [H]

Pfam domain/function: PF12161 HsdM_N; PF02384 N6_Mtase [H]

EC number: =2.1.1.72 [H]

Molecular weight: Translated: 54255; Mature: 54255

Theoretical pI: Translated: 4.56; Mature: 4.56

Prosite motif: PS00092 N6_MTASE

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.2 %Cys     (Translated Protein)
2.5 %Met     (Translated Protein)
3.7 %Cys+Met (Translated Protein)
1.2 %Cys     (Mature Protein)
2.5 %Met     (Mature Protein)
3.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNNNDLVAKLWKLCDNLRDGGVSYQNYVNELASLLFLKMCEETGQEDDLLPEGYRWADLK
CCCCHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCEEHHHH
AKLGQEQHQFYRNMLVQLGADDHAIVRAIFQNVNTTITQPAQLTELVDNMDKLEWFDGDT
HHHCHHHHHHHHHHHHHCCCCHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHEECCCCC
GKSRDDFGDMYEGLLQKNANETKSGAGQYFTPRSLISTIIKVMQPQPREVIQDPAAGTAG
CCCCHHHHHHHHHHHHCCCCCCCCCCCCCCCHHHHHHHHHHHHCCCCHHHHCCCCCCCCC
FLIEADKYIKSRTNDLDDLDDDDQEFQMTKAFVGLELVPETRRLALMNCLLHDIEGDAEE
EEEEHHHHHHHCCCCCCCCCCCHHHHHHHHHHHCCEECCHHHHHHHHHHHHHHHCCCCCC
GAIRLGNTLGSAGETLPQANVILTNPPFGSASSTNITRTFVHPTGNKQLCFMQHIYDALE
CCEEECCHHCCCCCCCCCCCEEEECCCCCCCCCCCEEEEEECCCCCCCCHHHHHHHHHHC
PGGRAAVVIPDNVLFEGGKGADIRRDLMDKCNLHTILRLPTGIFYAAGVKTNVLFFQKGT
CCCCEEEEECCCEEECCCCCCHHHHHHHHHCCCCEEECCCCCCEEECCCEEEEEEEECCC
QQDPNQDKGCTKETWVFDMRTNMNTFGKRRPLTEKHFDVFVNAYGSDTNGLSAREEGVYE
CCCCCCCCCCCCCCEEEEECCCCHHHHCCCCCCHHHHEEEEEECCCCCCCCCHHHHHHHH
TLGNIFAEGDDSVENLIENARFRKFSRDYIRDQKGDSLDISWLKDLEATSAENLPEPRAR
HHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHCCCCCCCCCCC
SASR
CCCC
>Mature Secondary Structure
MNNNDLVAKLWKLCDNLRDGGVSYQNYVNELASLLFLKMCEETGQEDDLLPEGYRWADLK
CCCCHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCEEHHHH
AKLGQEQHQFYRNMLVQLGADDHAIVRAIFQNVNTTITQPAQLTELVDNMDKLEWFDGDT
HHHCHHHHHHHHHHHHHCCCCHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHEECCCCC
GKSRDDFGDMYEGLLQKNANETKSGAGQYFTPRSLISTIIKVMQPQPREVIQDPAAGTAG
CCCCHHHHHHHHHHHHCCCCCCCCCCCCCCCHHHHHHHHHHHHCCCCHHHHCCCCCCCCC
FLIEADKYIKSRTNDLDDLDDDDQEFQMTKAFVGLELVPETRRLALMNCLLHDIEGDAEE
EEEEHHHHHHHCCCCCCCCCCCHHHHHHHHHHHCCEECCHHHHHHHHHHHHHHHCCCCCC
GAIRLGNTLGSAGETLPQANVILTNPPFGSASSTNITRTFVHPTGNKQLCFMQHIYDALE
CCEEECCHHCCCCCCCCCCCEEEECCCCCCCCCCCEEEEEECCCCCCCCHHHHHHHHHHC
PGGRAAVVIPDNVLFEGGKGADIRRDLMDKCNLHTILRLPTGIFYAAGVKTNVLFFQKGT
CCCCEEEEECCCEEECCCCCCHHHHHHHHHCCCCEEECCCCCCEEECCCEEEEEEEECCC
QQDPNQDKGCTKETWVFDMRTNMNTFGKRRPLTEKHFDVFVNAYGSDTNGLSAREEGVYE
CCCCCCCCCCCCCCEEEEECCCCHHHHCCCCCCHHHHEEEEEECCCCCCCCCHHHHHHHH
TLGNIFAEGDDSVENLIENARFRKFSRDYIRDQKGDSLDISWLKDLEATSAENLPEPRAR
HHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHCCCCCCCCCCC
SASR
CCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 3323532; 7610040; 9278503 [H]