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The map label for this gene is mii [H]

Identifier: 218709337

GI number: 218709337

Start: 1457470

End: 1458642

Strand: Direct

Name: mii [H]

Synonym: VS_1346

Alternate gene names: 218709337

Gene position: 1457470-1458642 (Clockwise)

Preceding gene: 218709336

Following gene: 218709338

Centisome position: 44.18

GC content: 48.93

Gene sequence:

>1173_bases
ATGAACATCAAACAGATCAAAGTGCCTGCAACCTATATGCGAGGCGGGACGAGCAAAGGCGTTTTCTTCAAGCTAAGTGA
TTTACCTGAAGCTGCGCAAGTCGCCGGAGAAGCACGAGACGCATTACTTCTGCGTGTGATTGGCAGCCCGGACCCATATG
GCAAGCAAACCGATGGTATGGGTGGTGCAACATCCAGTACCAGCAAAACCGTTATTGTATCGAAAAGCAGCAAAGCCGAT
CACGATGTTGATTACCTCTTTGGTCAAGTGGCAATAGATAAGCCGTTCGTTGATTGGAGCGGTAACTGCGGTAACTTGTC
TGGGGCAATTGGTCCGTTCGCTATCCAAAGTGGTTTGGTCGATTCAAGTCGTATTCCGGAAAACGGAGTGGTCGAGGTGC
GAGTATGGCAAGTGAACATAGAGAAAACCATTATTGTTCATGTACCTATCGCTGACGGTGAAGTCCAAGAGTTAGGTGAC
TTTGAGCTCGATGGTGTTACTTTCCCCGCCGCGGAGATCCAAGTCGATTTTCTAGATCCAGCCGATGCGAGTGGCTCTAT
GTTCCCAACAGGGAATGTTGTCGATTTTTTAGATATTCCCGAATTGGGCTGCATCAAAGCGACATACATCAATGCAGGAA
TACCAACCATTTTTGTTGATGCTGAATCGGTGGGTTATCAAGGCACAGAGCTTCAATCGGATATCAACAGCGATTCAAAA
GCCTTGGCATTGTTTGAATCCATCCGAGCACATGGCGCGGTTGCGATGGGGCTGATTGATTCTTTAGAACAAGCGGAATC
ACGCCAACACACACCGAAAGTCGCGTTTGTTGCTAAGCCTCAAGCGTACCAAGCCTCCAGTGGTAAATCGGTTGCTGTCG
AAGATACTGACCTTTTGGTGCGTGCTTTGTCTATGGGACAACTGCATCACGCCATGATGGGTACGGCAGCGGTCGCCATT
GCTTCAGCGGTGAGTGTGCCAGGTACGTTAGTGAACTTGGCAGTCGGTGAGGGTTCTCGAGATTTTGTGACCTTTGGTCA
TCCATCAGGAACCTTAAAAGTGGGTGCTAAAGCGATACAAGGCGAAAGCGGGTGGAAAATTGAGAGGGCGATTATGAGTC
GTAGTGCTCGAGTGATCATGGAAGGTGCCATCCGTGTGCCTTTTCCTAAGTAA

Upstream 100 bases:

>100_bases
GACCTGCCGACTAGACACCGCAGACGAAGTGAGTGTGTATAGCGCTGGTGGTGTGTTGCAACGTTTCGCCAAAGACTTTC
TCGCACAGTAGGAGCTGGTT

Downstream 100 bases:

>100_bases
AGCGATTGTCGTTAACGGTCAAATGTTCACGAACACAACGCTGAGAAAAGAGTGCACCCAAGTGACACAGCCAAAAGCAA
GACGCTTGGACTAATGGATA

Product: hypothetical protein

Products: NA

Alternate protein names: (R)-3-methylitaconate isomerase; 3-methylitaconate delta-isomerase [H]

Number of amino acids: Translated: 390; Mature: 390

Protein sequence:

>390_residues
MNIKQIKVPATYMRGGTSKGVFFKLSDLPEAAQVAGEARDALLLRVIGSPDPYGKQTDGMGGATSSTSKTVIVSKSSKAD
HDVDYLFGQVAIDKPFVDWSGNCGNLSGAIGPFAIQSGLVDSSRIPENGVVEVRVWQVNIEKTIIVHVPIADGEVQELGD
FELDGVTFPAAEIQVDFLDPADASGSMFPTGNVVDFLDIPELGCIKATYINAGIPTIFVDAESVGYQGTELQSDINSDSK
ALALFESIRAHGAVAMGLIDSLEQAESRQHTPKVAFVAKPQAYQASSGKSVAVEDTDLLVRALSMGQLHHAMMGTAAVAI
ASAVSVPGTLVNLAVGEGSRDFVTFGHPSGTLKVGAKAIQGESGWKIERAIMSRSARVIMEGAIRVPFPK

Sequences:

>Translated_390_residues
MNIKQIKVPATYMRGGTSKGVFFKLSDLPEAAQVAGEARDALLLRVIGSPDPYGKQTDGMGGATSSTSKTVIVSKSSKAD
HDVDYLFGQVAIDKPFVDWSGNCGNLSGAIGPFAIQSGLVDSSRIPENGVVEVRVWQVNIEKTIIVHVPIADGEVQELGD
FELDGVTFPAAEIQVDFLDPADASGSMFPTGNVVDFLDIPELGCIKATYINAGIPTIFVDAESVGYQGTELQSDINSDSK
ALALFESIRAHGAVAMGLIDSLEQAESRQHTPKVAFVAKPQAYQASSGKSVAVEDTDLLVRALSMGQLHHAMMGTAAVAI
ASAVSVPGTLVNLAVGEGSRDFVTFGHPSGTLKVGAKAIQGESGWKIERAIMSRSARVIMEGAIRVPFPK
>Mature_390_residues
MNIKQIKVPATYMRGGTSKGVFFKLSDLPEAAQVAGEARDALLLRVIGSPDPYGKQTDGMGGATSSTSKTVIVSKSSKAD
HDVDYLFGQVAIDKPFVDWSGNCGNLSGAIGPFAIQSGLVDSSRIPENGVVEVRVWQVNIEKTIIVHVPIADGEVQELGD
FELDGVTFPAAEIQVDFLDPADASGSMFPTGNVVDFLDIPELGCIKATYINAGIPTIFVDAESVGYQGTELQSDINSDSK
ALALFESIRAHGAVAMGLIDSLEQAESRQHTPKVAFVAKPQAYQASSGKSVAVEDTDLLVRALSMGQLHHAMMGTAAVAI
ASAVSVPGTLVNLAVGEGSRDFVTFGHPSGTLKVGAKAIQGESGWKIERAIMSRSARVIMEGAIRVPFPK

Specific function: Catalyzes the reversible isomerization of (R)-3- methylitaconate to 2,3-dimethylmaleate. Has very low isomerase activity with itaconate [H]

COG id: COG2828

COG function: function code S; Uncharacterized protein conserved in bacteria

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: NA

Homologues:

Organism=Escherichia coli, GI1786985, Length=382, Percent_Identity=33.2460732984293, Blast_Score=176, Evalue=3e-45,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR007400 [H]

Pfam domain/function: PF04303 PrpF [H]

EC number: =5.3.3.6 [H]

Molecular weight: Translated: 41049; Mature: 41049

Theoretical pI: Translated: 4.80; Mature: 4.80

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.5 %Cys     (Translated Protein)
2.6 %Met     (Translated Protein)
3.1 %Cys+Met (Translated Protein)
0.5 %Cys     (Mature Protein)
2.6 %Met     (Mature Protein)
3.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNIKQIKVPATYMRGGTSKGVFFKLSDLPEAAQVAGEARDALLLRVIGSPDPYGKQTDGM
CCCEEEECCHHHHCCCCCCCEEEEECCCCHHHHHHCCCCCEEEEEEECCCCCCCCCCCCC
GGATSSTSKTVIVSKSSKADHDVDYLFGQVAIDKPFVDWSGNCGNLSGAIGPFAIQSGLV
CCCCCCCCEEEEEECCCCCCCCHHHHEEEEEECCCCCCCCCCCCCCCCCCCCHHHHCCCC
DSSRIPENGVVEVRVWQVNIEKTIIVHVPIADGEVQELGDFELDGVTFPAAEIQVDFLDP
CCCCCCCCCEEEEEEEEEEECEEEEEEEECCCCCHHHCCCEEECCEECCCCEEEEEEECC
ADASGSMFPTGNVVDFLDIPELGCIKATYINAGIPTIFVDAESVGYQGTELQSDINSDSK
CCCCCCCCCCCCEEEEECCCCCCCEEEEEECCCCCEEEEEHHHCCCCCCHHHHHCCCCHH
ALALFESIRAHGAVAMGLIDSLEQAESRQHTPKVAFVAKPQAYQASSGKSVAVEDTDLLV
HHHHHHHHHHCCHHHHHHHHHHHHHHHHCCCCCEEEEECCCCEECCCCCEEEEEHHHHHH
RALSMGQLHHAMMGTAAVAIASAVSVPGTLVNLAVGEGSRDFVTFGHPSGTLKVGAKAIQ
HHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEEEEECCCCCCEEEECCCCCEEEECCEEEC
GESGWKIERAIMSRSARVIMEGAIRVPFPK
CCCCCCHHHHHHHCCCEEEEECCEECCCCC
>Mature Secondary Structure
MNIKQIKVPATYMRGGTSKGVFFKLSDLPEAAQVAGEARDALLLRVIGSPDPYGKQTDGM
CCCEEEECCHHHHCCCCCCCEEEEECCCCHHHHHHCCCCCEEEEEEECCCCCCCCCCCCC
GGATSSTSKTVIVSKSSKADHDVDYLFGQVAIDKPFVDWSGNCGNLSGAIGPFAIQSGLV
CCCCCCCCEEEEEECCCCCCCCHHHHEEEEEECCCCCCCCCCCCCCCCCCCCHHHHCCCC
DSSRIPENGVVEVRVWQVNIEKTIIVHVPIADGEVQELGDFELDGVTFPAAEIQVDFLDP
CCCCCCCCCEEEEEEEEEEECEEEEEEEECCCCCHHHCCCEEECCEECCCCEEEEEEECC
ADASGSMFPTGNVVDFLDIPELGCIKATYINAGIPTIFVDAESVGYQGTELQSDINSDSK
CCCCCCCCCCCCEEEEECCCCCCCEEEEEECCCCCEEEEEHHHCCCCCCHHHHHCCCCHH
ALALFESIRAHGAVAMGLIDSLEQAESRQHTPKVAFVAKPQAYQASSGKSVAVEDTDLLV
HHHHHHHHHHCCHHHHHHHHHHHHHHHHCCCCCEEEEECCCCEECCCCCEEEEEHHHHHH
RALSMGQLHHAMMGTAAVAIASAVSVPGTLVNLAVGEGSRDFVTFGHPSGTLKVGAKAIQ
HHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEEEEECCCCCCEEEECCCCCEEEECCEEEC
GESGWKIERAIMSRSARVIMEGAIRVPFPK
CCCCCCHHHHHHHCCCEEEEECCEECCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA