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The map label for this gene is fliM [H]

Identifier: 218708830

GI number: 218708830

Start: 852798

End: 853841

Strand: Direct

Name: fliM [H]

Synonym: VS_0830

Alternate gene names: 218708830

Gene position: 852798-853841 (Clockwise)

Preceding gene: 218708829

Following gene: 218708831

Centisome position: 25.85

GC content: 43.3

Gene sequence:

>1044_bases
GTGACCGATTTATTAAGCCAAGACGAAATTGATGCGCTATTACATGGCGTTGATGATGTTGAAGACGTTGAAGATGTCTT
AGAAACCGAAAGTGACAATACGGTCAATTTTGATTTCTCATCACAAGATCGAATCGTTCGTGGTCGAATGCCGACCCTTG
AACTTATTAACGAGCGTTTCGCACGCCATATGCGTATTAGCTTGTTTAATATGTTGCGTAAGACGGCTGAAGTGTCGATC
AACGGCGTACAGATGATGAAATTTGGTGAGTACCAGAACACACTCTATGTACCCACAAGTTTAAACATGGTTCGCTTCCG
CCCGTTAAAAGGTACTGCGCTAATCACGATGGAAGCTCGGCTAGTTTTCATTCTCGTAGAGAACTTTTTTGGTGGTGATG
GTCGTTTTCACGCCAAGATTGAAGGTCGCGAATTTACACCAACAGAAAGACGAATTATTCAGTTACTACTGAAAATTGTT
TTTGAAGATTACAAAGAAGCTTGGTCTCCCGTGATGGGCGTTGAGTTTGAATACTTGGACTCTGAAGTAAACCCAAGTAT
GGCTAACATTGTGAGCCCTACCGAAGTGATTGTTGTGAGTTCATTTCACATTGAAGTCGATGGCGGTGGCGGTGATTTCC
ATGTGGTTATGCCTTACTCAATGGTTGAGCCGATTCGTGAATTGCTGGATGCTGGTGTCCAATCAGATAAAATGGAAACC
GACGTTCGTTGGAGTTCAGCATTACGTGATGAAATCATGGATGTGCCAGTCAACTTCCGTGTCAATTTGCTCGAGCAAGA
TATCTCTTTGCGTGATTTGATGGAACTTCGTCCTGGGGATGTTATCCCGATGAATATGCCTGAGCATGCAACCATGTTTG
TTGAAGAACTGCCGACCTATCGTGTGAAAATGGGCCGTTCTGGAGAGAAGCTCGCGGTACAGATTTCTGAAAAAATTGAA
AGACCTCATGTGGTCAAAACCGATCTCGCTTTTCTTGGCAAAGACTTAATGTCTGAGTTGGAAAATAGCGAAGATGAAGA
ATAG

Upstream 100 bases:

>100_bases
AAGGCGACTGAAGATATTAAAGCAAGCTTAACTCAAGCTGTTGGCCAGCCGGTAATTGAAAAAGTGTTATTCACTGACTT
TGTAATTCAATAGGTAATAT

Downstream 100 bases:

>100_bases
CAAAGTAGATAAAAGTATAGGAATTGGTAATGGAACCTAGTGAAGATCAAAAGCTAGCAGACGAATGGGCTGCAGCACTT
GGTGAAGACCCTTCAGCGCC

Product: flagellar motor switch protein FliM

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 347; Mature: 346

Protein sequence:

>347_residues
MTDLLSQDEIDALLHGVDDVEDVEDVLETESDNTVNFDFSSQDRIVRGRMPTLELINERFARHMRISLFNMLRKTAEVSI
NGVQMMKFGEYQNTLYVPTSLNMVRFRPLKGTALITMEARLVFILVENFFGGDGRFHAKIEGREFTPTERRIIQLLLKIV
FEDYKEAWSPVMGVEFEYLDSEVNPSMANIVSPTEVIVVSSFHIEVDGGGGDFHVVMPYSMVEPIRELLDAGVQSDKMET
DVRWSSALRDEIMDVPVNFRVNLLEQDISLRDLMELRPGDVIPMNMPEHATMFVEELPTYRVKMGRSGEKLAVQISEKIE
RPHVVKTDLAFLGKDLMSELENSEDEE

Sequences:

>Translated_347_residues
MTDLLSQDEIDALLHGVDDVEDVEDVLETESDNTVNFDFSSQDRIVRGRMPTLELINERFARHMRISLFNMLRKTAEVSI
NGVQMMKFGEYQNTLYVPTSLNMVRFRPLKGTALITMEARLVFILVENFFGGDGRFHAKIEGREFTPTERRIIQLLLKIV
FEDYKEAWSPVMGVEFEYLDSEVNPSMANIVSPTEVIVVSSFHIEVDGGGGDFHVVMPYSMVEPIRELLDAGVQSDKMET
DVRWSSALRDEIMDVPVNFRVNLLEQDISLRDLMELRPGDVIPMNMPEHATMFVEELPTYRVKMGRSGEKLAVQISEKIE
RPHVVKTDLAFLGKDLMSELENSEDEE
>Mature_346_residues
TDLLSQDEIDALLHGVDDVEDVEDVLETESDNTVNFDFSSQDRIVRGRMPTLELINERFARHMRISLFNMLRKTAEVSIN
GVQMMKFGEYQNTLYVPTSLNMVRFRPLKGTALITMEARLVFILVENFFGGDGRFHAKIEGREFTPTERRIIQLLLKIVF
EDYKEAWSPVMGVEFEYLDSEVNPSMANIVSPTEVIVVSSFHIEVDGGGGDFHVVMPYSMVEPIRELLDAGVQSDKMETD
VRWSSALRDEIMDVPVNFRVNLLEQDISLRDLMELRPGDVIPMNMPEHATMFVEELPTYRVKMGRSGEKLAVQISEKIER
PHVVKTDLAFLGKDLMSELENSEDEE

Specific function: FliM is one of three proteins (FliG, FliN, FliM) that form the rotor-mounted switch complex (C ring), located at the base of the basal body. This complex interacts with the CheY and CheZ chemotaxis proteins, in addition to contacting components of the mot

COG id: COG1868

COG function: function code N; Flagellar motor switch protein

Gene ontology:

Cell location: Cell inner membrane; Peripheral membrane protein (Potential). Bacterial flagellum basal body [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the FliM family [H]

Homologues:

Organism=Escherichia coli, GI1788255, Length=317, Percent_Identity=37.2239747634069, Blast_Score=243, Evalue=9e-66,

Paralogues:

None

Copy number: 10-20 (rich media) [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001689
- InterPro:   IPR001543 [H]

Pfam domain/function: PF02154 FliM; PF01052 SpoA [H]

EC number: NA

Molecular weight: Translated: 39720; Mature: 39589

Theoretical pI: Translated: 4.31; Mature: 4.31

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
5.8 %Met     (Translated Protein)
5.8 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
5.5 %Met     (Mature Protein)
5.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTDLLSQDEIDALLHGVDDVEDVEDVLETESDNTVNFDFSSQDRIVRGRMPTLELINERF
CCCCCCHHHHHHHHHCCCCHHHHHHHHHCCCCCEEEEECCCCCCEEECCCCHHHHHHHHH
ARHMRISLFNMLRKTAEVSINGVQMMKFGEYQNTLYVPTSLNMVRFRPLKGTALITMEAR
HHHHHHHHHHHHHHHHHCCCCCEEEEEECCCCCEEEECCCCCEEEEECCCCCEEEEEEHE
LVFILVENFFGGDGRFHAKIEGREFTPTERRIIQLLLKIVFEDYKEAWSPVMGVEFEYLD
EEEEEEEHHCCCCCEEEEEECCCCCCHHHHHHHHHHHHHHHHHHHHHCCCCCCCCHHCCC
SEVNPSMANIVSPTEVIVVSSFHIEVDGGGGDFHVVMPYSMVEPIRELLDAGVQSDKMET
CCCCCCHHHHCCCCEEEEEEEEEEEEECCCCCEEEEECHHHHHHHHHHHHCCCCCCCCHH
DVRWSSALRDEIMDVPVNFRVNLLEQDISLRDLMELRPGDVIPMNMPEHATMFVEELPTY
HHHHHHHHHHHHHCCCCCEEEEEHHCCCCHHHHHHCCCCCEEECCCCHHHHHHHHHCCCE
RVKMGRSGEKLAVQISEKIERPHVVKTDLAFLGKDLMSELENSEDEE
EEECCCCCCEEEEEEHHHCCCCCEEHHHHHHHHHHHHHHHCCCCCCC
>Mature Secondary Structure 
TDLLSQDEIDALLHGVDDVEDVEDVLETESDNTVNFDFSSQDRIVRGRMPTLELINERF
CCCCCHHHHHHHHHCCCCHHHHHHHHHCCCCCEEEEECCCCCCEEECCCCHHHHHHHHH
ARHMRISLFNMLRKTAEVSINGVQMMKFGEYQNTLYVPTSLNMVRFRPLKGTALITMEAR
HHHHHHHHHHHHHHHHHCCCCCEEEEEECCCCCEEEECCCCCEEEEECCCCCEEEEEEHE
LVFILVENFFGGDGRFHAKIEGREFTPTERRIIQLLLKIVFEDYKEAWSPVMGVEFEYLD
EEEEEEEHHCCCCCEEEEEECCCCCCHHHHHHHHHHHHHHHHHHHHHCCCCCCCCHHCCC
SEVNPSMANIVSPTEVIVVSSFHIEVDGGGGDFHVVMPYSMVEPIRELLDAGVQSDKMET
CCCCCCHHHHCCCCEEEEEEEEEEEEECCCCCEEEEECHHHHHHHHHHHHCCCCCCCCHH
DVRWSSALRDEIMDVPVNFRVNLLEQDISLRDLMELRPGDVIPMNMPEHATMFVEELPTY
HHHHHHHHHHHHHCCCCCEEEEEHHCCCCHHHHHHCCCCCEEECCCCHHHHHHHHHCCCE
RVKMGRSGEKLAVQISEKIERPHVVKTDLAFLGKDLMSELENSEDEE
EEECCCCCCEEEEEEHHHCCCCCEEHHHHHHHHHHHHHHHCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 7.0

TargetDB status: NA

Availability: NA

References: 10984043; 7622217 [H]