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The map label for this gene is flgJ [H]

Identifier: 218708809

GI number: 218708809

Start: 826927

End: 827868

Strand: Direct

Name: flgJ [H]

Synonym: VS_0809

Alternate gene names: 218708809

Gene position: 826927-827868 (Clockwise)

Preceding gene: 218708808

Following gene: 218708810

Centisome position: 25.06

GC content: 44.37

Gene sequence:

>942_bases
ATGATTAAAAATAACAATGACATCGGCTTTATTCACGATATTGGTAGCCTAGACCGTCTTCGTCAACAAGCGGTAAGCGG
TGAAGAAGGCAGTGAAAAAGAAGCACTAACCGCTGCTGCAAAACAGTTTGAATCTATTTTTACCTCGATGTTGTTTAAGT
CGATGCGTGATGCGAACTCGAGCTTTAAGTCAGATATGTTGAACAGCCAGAATGAACAGTTTTATCGTCAGATGCAAGAT
GACCAAATGGCGAGTGAACTCAGTGCGTCAGGTTCGCTCGGTCTTGCGGACATGATCGTAGCTCAGTTAAGCGCTGGCCA
AGCAAATGATGCAACTGAAGATAAGGTTCGCAATGAAGGCTTTGATACTTCACTGCGAAGACCGCAATACTCGGGGCGTT
CAGAAGATAGAGTGTCGGATGTTCAATCTGCGCAAGCTATGTCTGCAACAGACGTGTCAGCAGCCAAACAACCCGTTTCC
TTCGATTCTCCAGAATCGTTTGTTGCCTCAATGAAGCCTTACGCTGAAAAAGCAGCGAGTGCGCTCGGTGTCGATTCATC
ACTGCTATTGGCACAAGCGGCACTTGAAACAGGTTGGGGTTCTAAGATGATTAAGAACTCTTTGGGCAACAGCAACAACC
TATTCAATATCAAAGCCGATAGAAGTTGGAAGGGCGATAAGGTTGCTACTCAAACTCTAGAGTTCCATGGTAAAACAGCC
GTTAAAGAGTCTGCTTCTTTCCGTTCTTACTCTAATTTCGAAGATAGCTTTAATGATTACGTGAAGTTCTTGAACGAAAA
CCCAAGATACGAAACGGCATTGCAGCATCAAGGTAACTCTGAGAATTTCATCAAAGGCATTCACCAGGCGGGTTACGCAA
CCGACCCTAACTATGCCGACAAGGTTTTACGAGTTAAAGCTAAGATTGATGAGATGAAATAG

Upstream 100 bases:

>100_bases
GAAGTCGGCGCCGCACCTTCTGATTTAATGGCAATCCTTCAAGCCCTGAAACAAGCGGGTGCAATTGAAGGTCAGTTGAT
CATTATTTAAGGGATAAAGC

Downstream 100 bases:

>100_bases
CGTTATTGAATGCACTCGAGAGCTTGCCACCGGCAGGCTCTTTTCTTATCCGCTCCTTCAGTCTTACGCGCCATCCATAG
TTTTCTCCTTACACTTCAAT

Product: flagellar rod assembly protein/muramidase FlgJ

Products: NA

Alternate protein names: Muramidase flgJ [H]

Number of amino acids: Translated: 313; Mature: 313

Protein sequence:

>313_residues
MIKNNNDIGFIHDIGSLDRLRQQAVSGEEGSEKEALTAAAKQFESIFTSMLFKSMRDANSSFKSDMLNSQNEQFYRQMQD
DQMASELSASGSLGLADMIVAQLSAGQANDATEDKVRNEGFDTSLRRPQYSGRSEDRVSDVQSAQAMSATDVSAAKQPVS
FDSPESFVASMKPYAEKAASALGVDSSLLLAQAALETGWGSKMIKNSLGNSNNLFNIKADRSWKGDKVATQTLEFHGKTA
VKESASFRSYSNFEDSFNDYVKFLNENPRYETALQHQGNSENFIKGIHQAGYATDPNYADKVLRVKAKIDEMK

Sequences:

>Translated_313_residues
MIKNNNDIGFIHDIGSLDRLRQQAVSGEEGSEKEALTAAAKQFESIFTSMLFKSMRDANSSFKSDMLNSQNEQFYRQMQD
DQMASELSASGSLGLADMIVAQLSAGQANDATEDKVRNEGFDTSLRRPQYSGRSEDRVSDVQSAQAMSATDVSAAKQPVS
FDSPESFVASMKPYAEKAASALGVDSSLLLAQAALETGWGSKMIKNSLGNSNNLFNIKADRSWKGDKVATQTLEFHGKTA
VKESASFRSYSNFEDSFNDYVKFLNENPRYETALQHQGNSENFIKGIHQAGYATDPNYADKVLRVKAKIDEMK
>Mature_313_residues
MIKNNNDIGFIHDIGSLDRLRQQAVSGEEGSEKEALTAAAKQFESIFTSMLFKSMRDANSSFKSDMLNSQNEQFYRQMQD
DQMASELSASGSLGLADMIVAQLSAGQANDATEDKVRNEGFDTSLRRPQYSGRSEDRVSDVQSAQAMSATDVSAAKQPVS
FDSPESFVASMKPYAEKAASALGVDSSLLLAQAALETGWGSKMIKNSLGNSNNLFNIKADRSWKGDKVATQTLEFHGKTA
VKESASFRSYSNFEDSFNDYVKFLNENPRYETALQHQGNSENFIKGIHQAGYATDPNYADKVLRVKAKIDEMK

Specific function: Flagellum-specific muramidase which hydrolyzes the peptidoglycan layer to assemble the rod structure in the periplasmic space [H]

COG id: COG1705

COG function: function code NU; Muramidase (flagellum-specific)

Gene ontology:

Cell location: Periplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: In the C-terminal section; belongs to the glycosyl hydrolase 73 family [H]

Homologues:

Organism=Escherichia coli, GI1787321, Length=304, Percent_Identity=34.2105263157895, Blast_Score=145, Evalue=2e-36,

Paralogues:

None

Copy number: 10-20 (rich media) [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR013377
- InterPro:   IPR000423
- InterPro:   IPR019301
- InterPro:   IPR013338
- InterPro:   IPR002901 [H]

Pfam domain/function: PF01832 Glucosaminidase; PF10135 Rod-binding [H]

EC number: 3.2.1.-

Molecular weight: Translated: 34453; Mature: 34453

Theoretical pI: Translated: 4.98; Mature: 4.98

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
3.5 %Met     (Translated Protein)
3.5 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
3.5 %Met     (Mature Protein)
3.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MIKNNNDIGFIHDIGSLDRLRQQAVSGEEGSEKEALTAAAKQFESIFTSMLFKSMRDANS
CCCCCCCEEEEECHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
SFKSDMLNSQNEQFYRQMQDDQMASELSASGSLGLADMIVAQLSAGQANDATEDKVRNEG
HHHHHHHCCHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHCCCCCCCHHHHHHHCC
FDTSLRRPQYSGRSEDRVSDVQSAQAMSATDVSAAKQPVSFDSPESFVASMKPYAEKAAS
CCCHHCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHH
ALGVDSSLLLAQAALETGWGSKMIKNSLGNSNNLFNIKADRSWKGDKVATQTLEFHGKTA
HHCCCHHHHHHHHHHHCCCCHHHHHHHCCCCCCEEEEECCCCCCCCHHHHHHHHHCCCHH
VKESASFRSYSNFEDSFNDYVKFLNENPRYETALQHQGNSENFIKGIHQAGYATDPNYAD
HHHHHHHHHHCCHHHHHHHHHHHHCCCCCHHHHHHCCCCCHHHHHHHHHCCCCCCCHHHH
KVLRVKAKIDEMK
HHHHHHHHHHCCC
>Mature Secondary Structure
MIKNNNDIGFIHDIGSLDRLRQQAVSGEEGSEKEALTAAAKQFESIFTSMLFKSMRDANS
CCCCCCCEEEEECHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
SFKSDMLNSQNEQFYRQMQDDQMASELSASGSLGLADMIVAQLSAGQANDATEDKVRNEG
HHHHHHHCCHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHCCCCCCCHHHHHHHCC
FDTSLRRPQYSGRSEDRVSDVQSAQAMSATDVSAAKQPVSFDSPESFVASMKPYAEKAAS
CCCHHCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHH
ALGVDSSLLLAQAALETGWGSKMIKNSLGNSNNLFNIKADRSWKGDKVATQTLEFHGKTA
HHCCCHHHHHHHHHHHCCCCHHHHHHHCCCCCCEEEEECCCCCCCCHHHHHHHHHCCCHH
VKESASFRSYSNFEDSFNDYVKFLNENPRYETALQHQGNSENFIKGIHQAGYATDPNYAD
HHHHHHHHHHCCHHHHHHHHHHHHCCCCCHHHHHHCCCCCHHHHHHHHHCCCCCCCHHHH
KVLRVKAKIDEMK
HHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 12620739 [H]