Click here to switch to the map view.

The map label for this gene is pth

Identifier: 218708750

GI number: 218708750

Start: 768223

End: 768813

Strand: Reverse

Name: pth

Synonym: VS_0747

Alternate gene names: 218708750

Gene position: 768813-768223 (Counterclockwise)

Preceding gene: 218708751

Following gene: 218708749

Centisome position: 23.3

GC content: 43.32

Gene sequence:

>591_bases
TTGAGCCAACAAATAAAACTTCTCGTTGGACTGGCTAATCCAGGTCCAGAATACGCCAAAACTCGCCACAATGCGGGTGC
TTGGGTAGTTGAAGAATTAGCACGTGTACACAACGTGACACTGAAGAACGAACCAAAGTTCTTTGGCCTAACGGGTCGTA
TCATGGTTCATGGTGAAGATCTTCGTTTGCTGATCCCAACGACTTTTATGAACTTATCCGGAAAAGCAGTTGCAGCACTG
GCAAAGTTCTACCAAATTAAACCAGAAGAGATCATGGTCGCTCACGATGAGTTAGACCTCCCTCCAGGTATTGGTAAGTT
TAAAAAAGGTGGTGGTCATGGTGGACATAATGGTCTGAAAGACATCATAAGCAAACAGGGTAACAATAAAGAATTCTATC
GTCTTAGATTAGGCATTGGCCATCCAGGACACAAAGATAAAGTTGCAGGTTATGTATTAGGCAAAGCTCCTGCGAAAGAG
CAAGAGTGTATCGAGGCTGTCGTTGACGAATCGGTTCGTAGCCTAGACATCTTATTGAAAGATGGCCTACCAAAAGCACA
AAATCGCTTACATACGTTCAAAGCAGAATAA

Upstream 100 bases:

>100_bases
CACAGTTTGAATCATTGAGTTGCTATCAGCTAGCTCGCACCTTTTTCATAAACTGTGATATCATACCGCGCTTTTTGAAA
TCCCAAGAGAGATCCATACC

Downstream 100 bases:

>100_bases
GGTTTATATCATGGGTTTTAAATGTGGCATCGTTGGTCTACCAAACGTTGGTAAGTCAACTCTGTTTAACGCACTGACTA
AAGCAGGCATCGAAGCAGCA

Product: peptidyl-tRNA hydrolase

Products: NA

Alternate protein names: PTH

Number of amino acids: Translated: 196; Mature: 195

Protein sequence:

>196_residues
MSQQIKLLVGLANPGPEYAKTRHNAGAWVVEELARVHNVTLKNEPKFFGLTGRIMVHGEDLRLLIPTTFMNLSGKAVAAL
AKFYQIKPEEIMVAHDELDLPPGIGKFKKGGGHGGHNGLKDIISKQGNNKEFYRLRLGIGHPGHKDKVAGYVLGKAPAKE
QECIEAVVDESVRSLDILLKDGLPKAQNRLHTFKAE

Sequences:

>Translated_196_residues
MSQQIKLLVGLANPGPEYAKTRHNAGAWVVEELARVHNVTLKNEPKFFGLTGRIMVHGEDLRLLIPTTFMNLSGKAVAAL
AKFYQIKPEEIMVAHDELDLPPGIGKFKKGGGHGGHNGLKDIISKQGNNKEFYRLRLGIGHPGHKDKVAGYVLGKAPAKE
QECIEAVVDESVRSLDILLKDGLPKAQNRLHTFKAE
>Mature_195_residues
SQQIKLLVGLANPGPEYAKTRHNAGAWVVEELARVHNVTLKNEPKFFGLTGRIMVHGEDLRLLIPTTFMNLSGKAVAALA
KFYQIKPEEIMVAHDELDLPPGIGKFKKGGGHGGHNGLKDIISKQGNNKEFYRLRLGIGHPGHKDKVAGYVLGKAPAKEQ
ECIEAVVDESVRSLDILLKDGLPKAQNRLHTFKAE

Specific function: The natural substrate for this enzyme may be peptidyl- tRNAs which drop off the ribosome during protein synthesis

COG id: COG0193

COG function: function code J; Peptidyl-tRNA hydrolase

Gene ontology:

Cell location: Cytoplasm

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the PTH family

Homologues:

Organism=Homo sapiens, GI50897284, Length=175, Percent_Identity=35.4285714285714, Blast_Score=91, Evalue=5e-19,
Organism=Escherichia coli, GI1787455, Length=194, Percent_Identity=65.979381443299, Blast_Score=248, Evalue=2e-67,
Organism=Saccharomyces cerevisiae, GI6321983, Length=173, Percent_Identity=29.4797687861272, Blast_Score=65, Evalue=7e-12,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): PTH_VIBSL (B7VKG8)

Other databases:

- EMBL:   FM954972
- RefSeq:   YP_002416371.1
- GeneID:   7160348
- GenomeReviews:   FM954972_GR
- KEGG:   vsp:VS_0747
- HOGENOM:   HBG610927
- OMA:   AMHRLHS
- ProtClustDB:   PRK05426
- GO:   GO:0005737
- GO:   GO:0006412
- HAMAP:   MF_00083
- InterPro:   IPR001328
- InterPro:   IPR018171
- Gene3D:   G3DSA:3.40.50.1470
- PANTHER:   PTHR17224
- TIGRFAMs:   TIGR00447

Pfam domain/function: PF01195 Pept_tRNA_hydro; SSF53178 Pept_tRNA_hydro

EC number: =3.1.1.29

Molecular weight: Translated: 21515; Mature: 21384

Theoretical pI: Translated: 9.94; Mature: 9.94

Prosite motif: PS01195 PEPT_TRNA_HYDROL_1; PS01196 PEPT_TRNA_HYDROL_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.5 %Cys     (Translated Protein)
2.0 %Met     (Translated Protein)
2.6 %Cys+Met (Translated Protein)
0.5 %Cys     (Mature Protein)
1.5 %Met     (Mature Protein)
2.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSQQIKLLVGLANPGPEYAKTRHNAGAWVVEELARVHNVTLKNEPKFFGLTGRIMVHGED
CCCCEEEEEEECCCCHHHHHHCCCCCHHHHHHHHHHHCCEECCCCCEEEEEEEEEEECCC
LRLLIPTTFMNLSGKAVAALAKFYQIKPEEIMVAHDELDLPPGIGKFKKGGGHGGHNGLK
EEEEECCCCCCCCCHHHHHHHHHHCCCCHHEEEECCCCCCCCCCCCCCCCCCCCCCHHHH
DIISKQGNNKEFYRLRLGIGHPGHKDKVAGYVLGKAPAKEQECIEAVVDESVRSLDILLK
HHHHCCCCCCEEEEEEEECCCCCCCCCEEEEEECCCCCCHHHHHHHHHHHHHHHHHHHHH
DGLPKAQNRLHTFKAE
CCCCHHHHHHECCCCC
>Mature Secondary Structure 
SQQIKLLVGLANPGPEYAKTRHNAGAWVVEELARVHNVTLKNEPKFFGLTGRIMVHGED
CCCEEEEEEECCCCHHHHHHCCCCCHHHHHHHHHHHCCEECCCCCEEEEEEEEEEECCC
LRLLIPTTFMNLSGKAVAALAKFYQIKPEEIMVAHDELDLPPGIGKFKKGGGHGGHNGLK
EEEEECCCCCCCCCHHHHHHHHHHCCCCHHEEEECCCCCCCCCCCCCCCCCCCCCCHHHH
DIISKQGNNKEFYRLRLGIGHPGHKDKVAGYVLGKAPAKEQECIEAVVDESVRSLDILLK
HHHHCCCCCCEEEEEEEECCCCCCCCCEEEEEECCCCCCHHHHHHHHHHHHHHHHHHHHH
DGLPKAQNRLHTFKAE
CCCCHHHHHHECCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA