Click here to switch to the map view.

The map label for this gene is mltF [H]

Identifier: 218708592

GI number: 218708592

Start: 583497

End: 584921

Strand: Reverse

Name: mltF [H]

Synonym: VS_0558

Alternate gene names: 218708592

Gene position: 584921-583497 (Counterclockwise)

Preceding gene: 218708593

Following gene: 218708591

Centisome position: 17.73

GC content: 42.39

Gene sequence:

>1425_bases
GTGGATGTGAGCAGGATGTTATTATTTATCTGGATCGGGTTATTTAGCCAACTTTCCAACGCATTAGAACTGTCCCCATT
GAGCAATCAACCTTACATGGGAGACTTAGATGTACTCAAAACAAAAGGAACAGTGAGAGTATTGGTTTCGGCTGATCTGG
GCTTTTACTACATAGAAGATGGCAAGCCAAAAGGTATTGTGGCAGAAATGCTCTACCACTTTGAGAAAAGTCTTCGTAAG
AAGCACCCCTACCTCAACGTTCAAATCATCCCCGTGCAACGAGATGATCTACTCCCCTCGTTAGAATCTGGTTACGGTGA
TGTTGCTGTCGCCAACCTAACGATCACCGACAAGCGATTAAGTGTCATCGATTTTTCCGATCCAATGATCAAAGATGGGA
AAGAACTGATTATCACCGGAAAAAATTCTGCCCCTATCACCGAAATCAAGCAATTAAGTGGTCAAGAGGTCTGGATCCGA
GCTAGCTCGAGTTACTTCGAGAGTGTTCAAAGAGTCAACAAAGAGCTTAACGCACTAGGTTTACCACCACTGCATGTCCA
CTTTATCGAAGAATCATTACAAGACTACGAGCTTATAGAACTGGTTAATCAGGGTTATATACAAGGAACAGTTCTAGATA
GCCACAAAGCTAAGCTCTGGCTCGATGTTATGGAAAACATTCAGGTACACACTGAGTTGCCGCTGCGTGAAAATGGTCAA
ATTGCTTGGGCACTGAGAAAAGACAGCCCTCAGCTAAAGAAAGAGATCAACAAATACGTAAAGACCGCACGAACAGGAAC
TCTACTTGGGAACGTTATCTATAATAAATACATCGACAACACTCGTTGGTTAGGTCGAGCTCTCAATCCAAACAAAGTCG
ACAGAGTTGCCAAGCTCGCTGACGTATTTGAGAAGTACTCCAGCAAGTACGAATTTGATCCTTTAATGATGTCTGCTCAA
GGGTTCCAAGAGTCAGGGCTCGATCAGAGTCAAGTATCTCATCGTGGGGCGATTGGCGTCATGCAGGTTTTACCAAGTAC
GGCCAAAGACAAAAACGTTAATATCAAAAATATTCATAAAGTGGATAACAACATCCATGCCGGCGTGAAATATATGCGCT
TTATCAAAGACCGATATTTTGACGATCCAGCGATATCGCCTGACAATCAAATCTACTTCACTTTAGCTTCTTACAATGCA
GGCCCCGCTAAGATAAGAAAGATGCGGAACATTGCTAAAAAGAAGGGTTATAACCCAAATATCTGGTTTAAAAACGTCGA
AATCATCACTCGTAAGTACGTGAGTAAAGAACCCGTAACCTATGTCGCCAACATCAATCGTTACTTCGTCATTTACAAAC
AATTGCAAGCAATCAATGCCATCCGAGAGGACAGTACAGCGAGATTACTAAACACTAACGATTAA

Upstream 100 bases:

>100_bases
TTAGCGCGATTTTTTTTGAACTTCGTCTGGTAAACTTGATGAATGGCAACATAAGCAGTATCTTCAATAGATAACTTTTA
ACAAAACATCAACATGGAGT

Downstream 100 bases:

>100_bases
CTTCCCCCTCAACATCAGAGCTTGAGGAACAAAGGTGTTAGATAAGGTTAAAAACATAAAAGCCGCACAAGAAAACTTAT
GCGGCTTTTGTTTATCTAAT

Product: amino-acid ABC transporter binding protein

Products: NA

Alternate protein names: Murein lyase F [H]

Number of amino acids: Translated: 474; Mature: 474

Protein sequence:

>474_residues
MDVSRMLLFIWIGLFSQLSNALELSPLSNQPYMGDLDVLKTKGTVRVLVSADLGFYYIEDGKPKGIVAEMLYHFEKSLRK
KHPYLNVQIIPVQRDDLLPSLESGYGDVAVANLTITDKRLSVIDFSDPMIKDGKELIITGKNSAPITEIKQLSGQEVWIR
ASSSYFESVQRVNKELNALGLPPLHVHFIEESLQDYELIELVNQGYIQGTVLDSHKAKLWLDVMENIQVHTELPLRENGQ
IAWALRKDSPQLKKEINKYVKTARTGTLLGNVIYNKYIDNTRWLGRALNPNKVDRVAKLADVFEKYSSKYEFDPLMMSAQ
GFQESGLDQSQVSHRGAIGVMQVLPSTAKDKNVNIKNIHKVDNNIHAGVKYMRFIKDRYFDDPAISPDNQIYFTLASYNA
GPAKIRKMRNIAKKKGYNPNIWFKNVEIITRKYVSKEPVTYVANINRYFVIYKQLQAINAIREDSTARLLNTND

Sequences:

>Translated_474_residues
MDVSRMLLFIWIGLFSQLSNALELSPLSNQPYMGDLDVLKTKGTVRVLVSADLGFYYIEDGKPKGIVAEMLYHFEKSLRK
KHPYLNVQIIPVQRDDLLPSLESGYGDVAVANLTITDKRLSVIDFSDPMIKDGKELIITGKNSAPITEIKQLSGQEVWIR
ASSSYFESVQRVNKELNALGLPPLHVHFIEESLQDYELIELVNQGYIQGTVLDSHKAKLWLDVMENIQVHTELPLRENGQ
IAWALRKDSPQLKKEINKYVKTARTGTLLGNVIYNKYIDNTRWLGRALNPNKVDRVAKLADVFEKYSSKYEFDPLMMSAQ
GFQESGLDQSQVSHRGAIGVMQVLPSTAKDKNVNIKNIHKVDNNIHAGVKYMRFIKDRYFDDPAISPDNQIYFTLASYNA
GPAKIRKMRNIAKKKGYNPNIWFKNVEIITRKYVSKEPVTYVANINRYFVIYKQLQAINAIREDSTARLLNTND
>Mature_474_residues
MDVSRMLLFIWIGLFSQLSNALELSPLSNQPYMGDLDVLKTKGTVRVLVSADLGFYYIEDGKPKGIVAEMLYHFEKSLRK
KHPYLNVQIIPVQRDDLLPSLESGYGDVAVANLTITDKRLSVIDFSDPMIKDGKELIITGKNSAPITEIKQLSGQEVWIR
ASSSYFESVQRVNKELNALGLPPLHVHFIEESLQDYELIELVNQGYIQGTVLDSHKAKLWLDVMENIQVHTELPLRENGQ
IAWALRKDSPQLKKEINKYVKTARTGTLLGNVIYNKYIDNTRWLGRALNPNKVDRVAKLADVFEKYSSKYEFDPLMMSAQ
GFQESGLDQSQVSHRGAIGVMQVLPSTAKDKNVNIKNIHKVDNNIHAGVKYMRFIKDRYFDDPAISPDNQIYFTLASYNA
GPAKIRKMRNIAKKKGYNPNIWFKNVEIITRKYVSKEPVTYVANINRYFVIYKQLQAINAIREDSTARLLNTND

Specific function: Murein-degrading enzyme that degrades murein glycan strands and insoluble, high-molecular weight murein sacculi, with the concomitant formation of a 1,6-anhydromuramoyl product. Lytic transglycosylases (LTs) play an integral role in the metabolism of the

COG id: COG4623

COG function: function code M; Predicted soluble lytic transglycosylase fused to an ABC-type amino acid-binding protein

Gene ontology:

Cell location: Cell outer membrane; Peripheral membrane protein. Note=Attached to the inner leaflet of the outer membrane [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: In the C-terminal section; belongs to the transglycosylase slt family [H]

Homologues:

Organism=Escherichia coli, GI171474010, Length=450, Percent_Identity=24.6666666666667, Blast_Score=72, Evalue=6e-14,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR008258
- InterPro:   IPR001638
- InterPro:   IPR000189 [H]

Pfam domain/function: PF00497 SBP_bac_3; PF01464 SLT [H]

EC number: NA

Molecular weight: Translated: 54082; Mature: 54082

Theoretical pI: Translated: 9.61; Mature: 9.61

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
2.3 %Met     (Translated Protein)
2.3 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
2.3 %Met     (Mature Protein)
2.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MDVSRMLLFIWIGLFSQLSNALELSPLSNQPYMGDLDVLKTKGTVRVLVSADLGFYYIED
CCHHHHHHHHHHHHHHHHHHHEEECCCCCCCCCCCHHHEECCCEEEEEEEECCCEEEEEC
GKPKGIVAEMLYHFEKSLRKKHPYLNVQIIPVQRDDLLPSLESGYGDVAVANLTITDKRL
CCCCHHHHHHHHHHHHHHHHHCCEEEEEEEEECHHHCCCCHHCCCCCEEEEEEEEECCEE
SVIDFSDPMIKDGKELIITGKNSAPITEIKQLSGQEVWIRASSSYFESVQRVNKELNALG
EEEECCCCHHCCCCEEEEECCCCCCHHHHHHCCCCEEEEEECHHHHHHHHHHHHHHHHCC
LPPLHVHFIEESLQDYELIELVNQGYIQGTVLDSHKAKLWLDVMENIQVHTELPLRENGQ
CCCHHHHHHHHHHHHHHHHHHHHCCCEEEEEECCCCHHHHHHHHHCCEEEEECCCCCCCC
IAWALRKDSPQLKKEINKYVKTARTGTLLGNVIYNKYIDNTRWLGRALNPNKVDRVAKLA
EEEEEECCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHCCCCCCHHHHHHHHHH
DVFEKYSSKYEFDPLMMSAQGFQESGLDQSQVSHRGAIGVMQVLPSTAKDKNVNIKNIHK
HHHHHHCCCCCCCHHHEECCCCHHCCCCHHHHHHCCCHHHHHHCCCCCCCCCCCHHHHHH
VDNNIHAGVKYMRFIKDRYFDDPAISPDNQIYFTLASYNAGPAKIRKMRNIAKKKGYNPN
HCCCHHHHHHHHHHHHHHCCCCCCCCCCCEEEEEEEECCCCHHHHHHHHHHHHHCCCCCC
IWFKNVEIITRKYVSKEPVTYVANINRYFVIYKQLQAINAIREDSTARLLNTND
EEEEHHHHHHHHHCCCCCEEEEECCCEEEEHHHHHHHHHHHHCCCCEEEECCCC
>Mature Secondary Structure
MDVSRMLLFIWIGLFSQLSNALELSPLSNQPYMGDLDVLKTKGTVRVLVSADLGFYYIED
CCHHHHHHHHHHHHHHHHHHHEEECCCCCCCCCCCHHHEECCCEEEEEEEECCCEEEEEC
GKPKGIVAEMLYHFEKSLRKKHPYLNVQIIPVQRDDLLPSLESGYGDVAVANLTITDKRL
CCCCHHHHHHHHHHHHHHHHHCCEEEEEEEEECHHHCCCCHHCCCCCEEEEEEEEECCEE
SVIDFSDPMIKDGKELIITGKNSAPITEIKQLSGQEVWIRASSSYFESVQRVNKELNALG
EEEECCCCHHCCCCEEEEECCCCCCHHHHHHCCCCEEEEEECHHHHHHHHHHHHHHHHCC
LPPLHVHFIEESLQDYELIELVNQGYIQGTVLDSHKAKLWLDVMENIQVHTELPLRENGQ
CCCHHHHHHHHHHHHHHHHHHHHCCCEEEEEECCCCHHHHHHHHHCCEEEEECCCCCCCC
IAWALRKDSPQLKKEINKYVKTARTGTLLGNVIYNKYIDNTRWLGRALNPNKVDRVAKLA
EEEEEECCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHCCCCCCHHHHHHHHHH
DVFEKYSSKYEFDPLMMSAQGFQESGLDQSQVSHRGAIGVMQVLPSTAKDKNVNIKNIHK
HHHHHHCCCCCCCHHHEECCCCHHCCCCHHHHHHCCCHHHHHHCCCCCCCCCCCHHHHHH
VDNNIHAGVKYMRFIKDRYFDDPAISPDNQIYFTLASYNAGPAKIRKMRNIAKKKGYNPN
HCCCHHHHHHHHHHHHHHCCCCCCCCCCCEEEEEEEECCCCHHHHHHHHHHHHHCCCCCC
IWFKNVEIITRKYVSKEPVTYVANINRYFVIYKQLQAINAIREDSTARLLNTND
EEEEHHHHHHHHHCCCCCEEEEECCCEEEEHHHHHHHHHHHHCCCCEEEECCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: NA