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The map label for this gene is oatA [H]

Identifier: 218708527

GI number: 218708527

Start: 513200

End: 515212

Strand: Reverse

Name: oatA [H]

Synonym: VS_0492

Alternate gene names: 218708527

Gene position: 515212-513200 (Counterclockwise)

Preceding gene: 218708530

Following gene: 218708526

Centisome position: 15.62

GC content: 36.61

Gene sequence:

>2013_bases
ATGTTGTCTTATCGTAAAGAGCTTGAAGGTCTTAGAGCTTTAGCTGTTATTGCTGTGATTATCTATCACGCCAACTTAAA
ACTCTTTGGAGTTCAGTTATTCCAAGGTGGTTTTTTTGGGGTGGATGTTTTTTTTGTTCTATCCGGATATCTGATCACTG
GAATAATTCGCCATAAGATGGAGCAAGGAACATTTTCATTTCGAGATTTTTATTGGCGAAGAGCTAAAAGAATTGTACCA
GCTTTCTTGACCATGTTGCTAATCACATCTGGTTTAGCTTACCTAATTTTATTACCTGACGACTTAGTTGTTTATGCTAA
ATCTCTCCAATCAGCTTTGTATTTAGGCTCTAATCACTTTTTTTATAGTGAGGACAGTTACACTGCGGCAGCTAGTATTT
ACCGTCCTTTACTTCACACATGGAGCTTATCGGTTGAGTGGCAGTTCTATGTTGTATTCCCATTTGTAATTTGGGTAATC
AATCGTTTTTTCCCAAGATACATGTTCGGACTACTCCTTGCTTTAGCCTTAGTATCCTTGCAAATTTCTAGCTTTATTGT
GAAAGTTAATCCCGATATGGCTTTCTATCTACTTCCTACACGAGCATGGGAGCTAATACTTGGTGGTTTGGTTACTTTTT
ATAATCGGACAAACATTGGAAGCTCAGCCAAAGGTAGCGTTGCATCCATCGCATACCAATCATTACCTTTAATTGGGCTA
TTTATAGTCGTTCACTCAATGGTCTTTATTGGTCATAACGTTCCTCATCCATCATTCATTACTCTGTTACCTGTTTTGGG
GACATGCCTTTTCATCATGTTCTCCCACAAAGGAGATGTAACTCATGAAGCAATGTCTTTAAAACCTGTTGTTGGAATAG
GTCTGGTCTCTTACTCGCTATATCTATGGCATCAGCCAGTATTTGTATTTTTTAGGTTTATTAAGCATGACTACTTTAGA
TATGAACAGTTTTTACTGTTAGTCCTTGTTTCTTTCGCATTTGCAATATGCAGTTATTTTTTTGTTGAAAATCCTTTTAG
GAAAAAAGAAATTTCTAGGAAAAAAATAATTTTCATTTTGATTGTGCTACTTATTTCGTCTGCGTTCAGTTTCTTTGTTC
AAAAACAACGAGGGTACCCAGAAAGACTTGGAGTAGTTACAACTCTTTTTAAAGATCTTAATGATAATTCCAACTTTAAG
ATGAATGGTATTCGTTGTCATAATCAACCATTCGATAAATCCTGCAGGGTTATGAGTGACAAAAATAATGTATTGTTACT
TGGTGACAGCCATGCAGGAAGCATTGGAAAACACTTATATAATGAATCTTTAAAACAAGGGATGGGATATATAAACCTAA
CCCCCTCAGGATGTACTGGAATTAATACAGTGAGGGTGAAGCGTAAAATAAAAGGTGAAGTTGTAAGGAAAAAACCTTGT
GAAGTAGCATCTGAGAAGATTTCAGCGTTTTTAGAAAGCCCAACTACACCGAAGATGAAAATTGTGTTTGTGACGAGGTT
ACCACAGTATTTGAGTGGAAAAGTCTTTAATAACGGCTTAGGGGGACTTGAGGCCGGTGAGGATTATTGGATCGAAGCAA
ATAACGGTGGAAGTGCGAAACAAGCAGTTCTCGACAAGCTATCTTTTTGGGCTTCTTTAGGGCATGAATTAGTTTTGGTT
TATCCGATCCCTGAAATGGGGTGGGATATACCTAAAAGGATCCAACAAATTTTAGGTGAGCATGTTTTACCAAAATCGAA
AAAAGATGCATATAATAATTTGATTATCAGTGTTCCAGAAACAACGTATCAAAAGAGGACATCGGAAGCTTTTGATTTGT
TGAATAACGTAGAAGGCAAGAACGTTTCTAGGGTTTATCCGCATAAAGTGTTTTGTACAGAAGGTCATTGTGCAGCGAAT
GATGCTACATCAATTTATTATTACGATGATGACCATTTGTCTACACATGGTGCCAAACTCTTAGTAAATGAAATAGCTAA
ATATTTACGATAA

Upstream 100 bases:

>100_bases
AAGCGAAATCAACTAGCTGCCGAACTGACATTTAGCATACCTGGCTAATGCCTTATCTTTTCGTTACAATACAGCCAAAA
ATTTTAAATGCTGAATATAT

Downstream 100 bases:

>100_bases
ATCTCGTTGTACGAACTAAATCAAGGGGCTAAACCATCAGCCTCTTTTTTTATATTTCTGGAAAGCTTTGCAAGCTCATC
GTAACCCCCAGTATTCAATC

Product: putative acyltransferase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 670; Mature: 670

Protein sequence:

>670_residues
MLSYRKELEGLRALAVIAVIIYHANLKLFGVQLFQGGFFGVDVFFVLSGYLITGIIRHKMEQGTFSFRDFYWRRAKRIVP
AFLTMLLITSGLAYLILLPDDLVVYAKSLQSALYLGSNHFFYSEDSYTAAASIYRPLLHTWSLSVEWQFYVVFPFVIWVI
NRFFPRYMFGLLLALALVSLQISSFIVKVNPDMAFYLLPTRAWELILGGLVTFYNRTNIGSSAKGSVASIAYQSLPLIGL
FIVVHSMVFIGHNVPHPSFITLLPVLGTCLFIMFSHKGDVTHEAMSLKPVVGIGLVSYSLYLWHQPVFVFFRFIKHDYFR
YEQFLLLVLVSFAFAICSYFFVENPFRKKEISRKKIIFILIVLLISSAFSFFVQKQRGYPERLGVVTTLFKDLNDNSNFK
MNGIRCHNQPFDKSCRVMSDKNNVLLLGDSHAGSIGKHLYNESLKQGMGYINLTPSGCTGINTVRVKRKIKGEVVRKKPC
EVASEKISAFLESPTTPKMKIVFVTRLPQYLSGKVFNNGLGGLEAGEDYWIEANNGGSAKQAVLDKLSFWASLGHELVLV
YPIPEMGWDIPKRIQQILGEHVLPKSKKDAYNNLIISVPETTYQKRTSEAFDLLNNVEGKNVSRVYPHKVFCTEGHCAAN
DATSIYYYDDDHLSTHGAKLLVNEIAKYLR

Sequences:

>Translated_670_residues
MLSYRKELEGLRALAVIAVIIYHANLKLFGVQLFQGGFFGVDVFFVLSGYLITGIIRHKMEQGTFSFRDFYWRRAKRIVP
AFLTMLLITSGLAYLILLPDDLVVYAKSLQSALYLGSNHFFYSEDSYTAAASIYRPLLHTWSLSVEWQFYVVFPFVIWVI
NRFFPRYMFGLLLALALVSLQISSFIVKVNPDMAFYLLPTRAWELILGGLVTFYNRTNIGSSAKGSVASIAYQSLPLIGL
FIVVHSMVFIGHNVPHPSFITLLPVLGTCLFIMFSHKGDVTHEAMSLKPVVGIGLVSYSLYLWHQPVFVFFRFIKHDYFR
YEQFLLLVLVSFAFAICSYFFVENPFRKKEISRKKIIFILIVLLISSAFSFFVQKQRGYPERLGVVTTLFKDLNDNSNFK
MNGIRCHNQPFDKSCRVMSDKNNVLLLGDSHAGSIGKHLYNESLKQGMGYINLTPSGCTGINTVRVKRKIKGEVVRKKPC
EVASEKISAFLESPTTPKMKIVFVTRLPQYLSGKVFNNGLGGLEAGEDYWIEANNGGSAKQAVLDKLSFWASLGHELVLV
YPIPEMGWDIPKRIQQILGEHVLPKSKKDAYNNLIISVPETTYQKRTSEAFDLLNNVEGKNVSRVYPHKVFCTEGHCAAN
DATSIYYYDDDHLSTHGAKLLVNEIAKYLR
>Mature_670_residues
MLSYRKELEGLRALAVIAVIIYHANLKLFGVQLFQGGFFGVDVFFVLSGYLITGIIRHKMEQGTFSFRDFYWRRAKRIVP
AFLTMLLITSGLAYLILLPDDLVVYAKSLQSALYLGSNHFFYSEDSYTAAASIYRPLLHTWSLSVEWQFYVVFPFVIWVI
NRFFPRYMFGLLLALALVSLQISSFIVKVNPDMAFYLLPTRAWELILGGLVTFYNRTNIGSSAKGSVASIAYQSLPLIGL
FIVVHSMVFIGHNVPHPSFITLLPVLGTCLFIMFSHKGDVTHEAMSLKPVVGIGLVSYSLYLWHQPVFVFFRFIKHDYFR
YEQFLLLVLVSFAFAICSYFFVENPFRKKEISRKKIIFILIVLLISSAFSFFVQKQRGYPERLGVVTTLFKDLNDNSNFK
MNGIRCHNQPFDKSCRVMSDKNNVLLLGDSHAGSIGKHLYNESLKQGMGYINLTPSGCTGINTVRVKRKIKGEVVRKKPC
EVASEKISAFLESPTTPKMKIVFVTRLPQYLSGKVFNNGLGGLEAGEDYWIEANNGGSAKQAVLDKLSFWASLGHELVLV
YPIPEMGWDIPKRIQQILGEHVLPKSKKDAYNNLIISVPETTYQKRTSEAFDLLNNVEGKNVSRVYPHKVFCTEGHCAAN
DATSIYYYDDDHLSTHGAKLLVNEIAKYLR

Specific function: Responsible for O-acetylation at the C(6)-hydroxyl group of N-acetylmuramyl residues, forming the corresponding N,6-O- diacetylmuramic acid of the peptidoglycan. O-acetylation of the peptidoglycan is the major determinant for lysozyme resistance [H]

COG id: COG1835

COG function: function code I; Predicted acyltransferases

Gene ontology:

Cell location: Cell membrane; Multi-pass membrane protein [H]

Metaboloic importance: NA

Operon status: Not Known

Operon components: None

Similarity: Belongs to the acyltransferase 3 family [H]

Homologues:

Organism=Caenorhabditis elegans, GI212640769, Length=486, Percent_Identity=26.5432098765432, Blast_Score=120, Evalue=3e-27,
Organism=Caenorhabditis elegans, GI133903927, Length=530, Percent_Identity=26.7924528301887, Blast_Score=116, Evalue=3e-26,
Organism=Caenorhabditis elegans, GI17507283, Length=393, Percent_Identity=27.9898218829517, Blast_Score=113, Evalue=4e-25,
Organism=Caenorhabditis elegans, GI17507733, Length=343, Percent_Identity=27.9883381924198, Blast_Score=112, Evalue=5e-25,
Organism=Caenorhabditis elegans, GI17507735, Length=339, Percent_Identity=28.9085545722714, Blast_Score=111, Evalue=1e-24,
Organism=Caenorhabditis elegans, GI17541014, Length=525, Percent_Identity=24.952380952381, Blast_Score=110, Evalue=3e-24,
Organism=Caenorhabditis elegans, GI17559158, Length=382, Percent_Identity=27.2251308900524, Blast_Score=108, Evalue=6e-24,
Organism=Caenorhabditis elegans, GI17541016, Length=379, Percent_Identity=27.4406332453826, Blast_Score=108, Evalue=1e-23,
Organism=Caenorhabditis elegans, GI17542538, Length=396, Percent_Identity=27.020202020202, Blast_Score=105, Evalue=5e-23,
Organism=Caenorhabditis elegans, GI71990417, Length=384, Percent_Identity=27.34375, Blast_Score=105, Evalue=6e-23,
Organism=Caenorhabditis elegans, GI193208811, Length=339, Percent_Identity=26.8436578171091, Blast_Score=104, Evalue=1e-22,
Organism=Caenorhabditis elegans, GI17541412, Length=344, Percent_Identity=29.9418604651163, Blast_Score=104, Evalue=2e-22,
Organism=Caenorhabditis elegans, GI17507287, Length=321, Percent_Identity=31.7757009345794, Blast_Score=103, Evalue=3e-22,
Organism=Caenorhabditis elegans, GI17507299, Length=389, Percent_Identity=29.0488431876607, Blast_Score=103, Evalue=3e-22,
Organism=Caenorhabditis elegans, GI17507289, Length=389, Percent_Identity=29.0488431876607, Blast_Score=103, Evalue=3e-22,
Organism=Caenorhabditis elegans, GI71984481, Length=432, Percent_Identity=27.5462962962963, Blast_Score=103, Evalue=4e-22,
Organism=Caenorhabditis elegans, GI17509001, Length=329, Percent_Identity=28.5714285714286, Blast_Score=102, Evalue=8e-22,
Organism=Caenorhabditis elegans, GI17541414, Length=364, Percent_Identity=28.5714285714286, Blast_Score=101, Evalue=1e-21,
Organism=Caenorhabditis elegans, GI193203179, Length=322, Percent_Identity=27.639751552795, Blast_Score=100, Evalue=3e-21,
Organism=Caenorhabditis elegans, GI17539118, Length=211, Percent_Identity=33.175355450237, Blast_Score=100, Evalue=4e-21,
Organism=Caenorhabditis elegans, GI17562864, Length=375, Percent_Identity=27.2, Blast_Score=96, Evalue=5e-20,
Organism=Caenorhabditis elegans, GI17507167, Length=344, Percent_Identity=28.1976744186047, Blast_Score=96, Evalue=7e-20,
Organism=Caenorhabditis elegans, GI17560876, Length=350, Percent_Identity=27.1428571428571, Blast_Score=95, Evalue=1e-19,
Organism=Caenorhabditis elegans, GI86562056, Length=316, Percent_Identity=25, Blast_Score=94, Evalue=2e-19,
Organism=Caenorhabditis elegans, GI193203181, Length=312, Percent_Identity=26.2820512820513, Blast_Score=94, Evalue=2e-19,
Organism=Caenorhabditis elegans, GI17562866, Length=333, Percent_Identity=26.7267267267267, Blast_Score=94, Evalue=2e-19,
Organism=Caenorhabditis elegans, GI17540232, Length=432, Percent_Identity=25.6944444444444, Blast_Score=91, Evalue=1e-18,
Organism=Caenorhabditis elegans, GI17559544, Length=313, Percent_Identity=25.8785942492013, Blast_Score=91, Evalue=3e-18,
Organism=Caenorhabditis elegans, GI17532481, Length=330, Percent_Identity=27.5757575757576, Blast_Score=90, Evalue=4e-18,
Organism=Caenorhabditis elegans, GI17542762, Length=340, Percent_Identity=28.2352941176471, Blast_Score=90, Evalue=4e-18,
Organism=Caenorhabditis elegans, GI17562856, Length=513, Percent_Identity=23.5867446393762, Blast_Score=89, Evalue=1e-17,
Organism=Caenorhabditis elegans, GI17543924, Length=475, Percent_Identity=25.8947368421053, Blast_Score=88, Evalue=1e-17,
Organism=Caenorhabditis elegans, GI32567150, Length=255, Percent_Identity=28.2352941176471, Blast_Score=87, Evalue=3e-17,
Organism=Caenorhabditis elegans, GI115534354, Length=333, Percent_Identity=28.5285285285285, Blast_Score=86, Evalue=4e-17,
Organism=Caenorhabditis elegans, GI71983744, Length=334, Percent_Identity=27.8443113772455, Blast_Score=83, Evalue=5e-16,
Organism=Caenorhabditis elegans, GI71988046, Length=343, Percent_Identity=25.9475218658892, Blast_Score=82, Evalue=7e-16,
Organism=Caenorhabditis elegans, GI17506687, Length=321, Percent_Identity=26.791277258567, Blast_Score=82, Evalue=8e-16,
Organism=Caenorhabditis elegans, GI212646204, Length=315, Percent_Identity=24.1269841269841, Blast_Score=80, Evalue=3e-15,
Organism=Caenorhabditis elegans, GI212642133, Length=318, Percent_Identity=29.559748427673, Blast_Score=77, Evalue=4e-14,
Organism=Caenorhabditis elegans, GI71982248, Length=331, Percent_Identity=25.3776435045317, Blast_Score=75, Evalue=1e-13,
Organism=Caenorhabditis elegans, GI193208625, Length=395, Percent_Identity=26.8354430379747, Blast_Score=74, Evalue=2e-13,
Organism=Caenorhabditis elegans, GI17531875, Length=344, Percent_Identity=27.0348837209302, Blast_Score=73, Evalue=4e-13,
Organism=Caenorhabditis elegans, GI193206646, Length=310, Percent_Identity=28.3870967741935, Blast_Score=66, Evalue=6e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR002656
- InterPro:   IPR013830
- InterPro:   IPR013831 [H]

Pfam domain/function: PF01757 Acyl_transf_3 [H]

EC number: NA

Molecular weight: Translated: 76043; Mature: 76043

Theoretical pI: Translated: 9.66; Mature: 9.66

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.2 %Cys     (Translated Protein)
1.9 %Met     (Translated Protein)
3.1 %Cys+Met (Translated Protein)
1.2 %Cys     (Mature Protein)
1.9 %Met     (Mature Protein)
3.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MLSYRKELEGLRALAVIAVIIYHANLKLFGVQLFQGGFFGVDVFFVLSGYLITGIIRHKM
CCCHHHHHHHHHHHHHHHHHHHHCCHHEEEEEHHCCCCHHHHHHHHHHHHHHHHHHHHHH
EQGTFSFRDFYWRRAKRIVPAFLTMLLITSGLAYLILLPDDLVVYAKSLQSALYLGSNHF
HCCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCEEEEECCCHHHHHHHHHHHHHHCCCCCE
FYSEDSYTAAASIYRPLLHTWSLSVEWQFYVVFPFVIWVINRFFPRYMFGLLLALALVSL
EECCCCHHHHHHHHHHHHHHHEEEEEEEEEEHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
QISSFIVKVNPDMAFYLLPTRAWELILGGLVTFYNRTNIGSSAKGSVASIAYQSLPLIGL
HHHHEEEEECCCEEEEEECHHHHHHHHHHHHHHHHCCCCCCCCCCCHHHHHHHHHHHHHH
FIVVHSMVFIGHNVPHPSFITLLPVLGTCLFIMFSHKGDVTHEAMSLKPVVGIGLVSYSL
HHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHCCCCCCCHHHHCCCCHHHHHHHHHHH
YLWHQPVFVFFRFIKHDYFRYEQFLLLVLVSFAFAICSYFFVENPFRKKEISRKKIIFIL
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHH
IVLLISSAFSFFVQKQRGYPERLGVVTTLFKDLNDNSNFKMNGIRCHNQPFDKSCRVMSD
HHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHCCCCCCEEECCEEECCCCCCCCCCEECC
KNNVLLLGDSHAGSIGKHLYNESLKQGMGYINLTPSGCTGINTVRVKRKIKGEVVRKKPC
CCCEEEEECCCCCHHHHHHHHHHHHHCCCEEEECCCCCCCCHHHHHHHHHHHHHHHCCCH
EVASEKISAFLESPTTPKMKIVFVTRLPQYLSGKVFNNGLGGLEAGEDYWIEANNGGSAK
HHHHHHHHHHHCCCCCCCEEEEEEECCCHHHCCCHHCCCCCCCCCCCCEEEEECCCCCHH
QAVLDKLSFWASLGHELVLVYPIPEMGWDIPKRIQQILGEHVLPKSKKDAYNNLIISVPE
HHHHHHHHHHHHCCCCEEEEEECCCCCCCHHHHHHHHHHHHCCCCCCCCCCCCEEEECCC
TTYQKRTSEAFDLLNNVEGKNVSRVYPHKVFCTEGHCAANDATSIYYYDDDHLSTHGAKL
HHHHHHHHHHHHHHHCCCCCCCCEECCCEEEEECCCCCCCCCCEEEEEECCCCCCCHHHH
LVNEIAKYLR
HHHHHHHHHC
>Mature Secondary Structure
MLSYRKELEGLRALAVIAVIIYHANLKLFGVQLFQGGFFGVDVFFVLSGYLITGIIRHKM
CCCHHHHHHHHHHHHHHHHHHHHCCHHEEEEEHHCCCCHHHHHHHHHHHHHHHHHHHHHH
EQGTFSFRDFYWRRAKRIVPAFLTMLLITSGLAYLILLPDDLVVYAKSLQSALYLGSNHF
HCCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCEEEEECCCHHHHHHHHHHHHHHCCCCCE
FYSEDSYTAAASIYRPLLHTWSLSVEWQFYVVFPFVIWVINRFFPRYMFGLLLALALVSL
EECCCCHHHHHHHHHHHHHHHEEEEEEEEEEHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
QISSFIVKVNPDMAFYLLPTRAWELILGGLVTFYNRTNIGSSAKGSVASIAYQSLPLIGL
HHHHEEEEECCCEEEEEECHHHHHHHHHHHHHHHHCCCCCCCCCCCHHHHHHHHHHHHHH
FIVVHSMVFIGHNVPHPSFITLLPVLGTCLFIMFSHKGDVTHEAMSLKPVVGIGLVSYSL
HHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHCCCCCCCHHHHCCCCHHHHHHHHHHH
YLWHQPVFVFFRFIKHDYFRYEQFLLLVLVSFAFAICSYFFVENPFRKKEISRKKIIFIL
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHH
IVLLISSAFSFFVQKQRGYPERLGVVTTLFKDLNDNSNFKMNGIRCHNQPFDKSCRVMSD
HHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHCCCCCCEEECCEEECCCCCCCCCCEECC
KNNVLLLGDSHAGSIGKHLYNESLKQGMGYINLTPSGCTGINTVRVKRKIKGEVVRKKPC
CCCEEEEECCCCCHHHHHHHHHHHHHCCCEEEECCCCCCCCHHHHHHHHHHHHHHHCCCH
EVASEKISAFLESPTTPKMKIVFVTRLPQYLSGKVFNNGLGGLEAGEDYWIEANNGGSAK
HHHHHHHHHHHCCCCCCCEEEEEEECCCHHHCCCHHCCCCCCCCCCCCEEEEECCCCCHH
QAVLDKLSFWASLGHELVLVYPIPEMGWDIPKRIQQILGEHVLPKSKKDAYNNLIISVPE
HHHHHHHHHHHHCCCCEEEEEECCCCCCCHHHHHHHHHHHHCCCCCCCCCCCCEEEECCC
TTYQKRTSEAFDLLNNVEGKNVSRVYPHKVFCTEGHCAANDATSIYYYDDDHLSTHGAKL
HHHHHHHHHHHHHHHCCCCCCCCEECCCEEEEECCCCCCCCCCEEEEEECCCCCCCHHHH
LVNEIAKYLR
HHHHHHHHHC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: NA