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The map label for this gene is btuF [H]

Identifier: 218708502

GI number: 218708502

Start: 481782

End: 482651

Strand: Reverse

Name: btuF [H]

Synonym: VS_0466

Alternate gene names: 218708502

Gene position: 482651-481782 (Counterclockwise)

Preceding gene: 218708503

Following gene: 218708501

Centisome position: 14.63

GC content: 44.94

Gene sequence:

>870_bases
GTGAAACCTTCAGCACTTGTTACAAGCCTTACTATTTTGTTTTCCAGCTTATGGCTGCCTTTGTCATTTGCAGAAGAAAC
ACTACTGACTTCAGAAAAAGTTCAACCAGCTCAACGTGTGGTTAGCCTTGCTCCTCATGCCACTGAACTCGCATACAGCG
CTGGTTTAGGTGATAAGCTTGTTGCGGTGAGTGAGCGAAGTGATTATCCACTACAAGCTGACAAACTAGAGAAAGTGGCG
AATTACCAAGGCATCAAGGTTGAGAAGATCATCGCCCTGCAACCTGATCTCATTCTCGCATGGCCAGCTGGAAACCCGCC
CAGAGAACTCGCGAAACTGGAGCAATTTGGTTTTAATATTTATTACTCAAAAACCAAGAGCCTAGATAACATCGCGACCA
ATATTGAGCAACTTAGCCAATTCGCAAGCGATCCAAGTATTGGCGAAAATAACGCTCAACAATACAGAGAGCAGCTCAAC
GCGTTGAGACTAAAATACAAAGATGCCGAGCCAGTAAACTACTTCTACCAACTCAGTGAGAAGCCGATCATCACTGTTGC
TCAAGGGCACTGGCCAAGTGAGGTGTTTGAGTTTTGTGGTGGCCACAATATTTTCGAAGACAGTGCTTCCCCTTACCCGC
AAGTTGGCATTGAACAAGTTGTGTTAAGGAAGCCGCAAGTGATCTTCACTTCTCAGCACGCGATTGAGAATGGAACCATG
TGGCAAACTTGGGAAGATGAGATCCCGGCAGTTGCTCAAAATCAGATTTGGTCACTTAATTCTGACTGGATTAATCGCCC
GACAACAAGAACTTTGAAAGCAATCCAACAAGTGTGCGATTTCTTCGATAGAGCTAGGCAAAATCACTAA

Upstream 100 bases:

>100_bases
CTGCATCTGTCTCAGGTTCAGAAACTACTCTTTGGTCGCATCGCTATCTGGATATTTTTACAAAGCCTAGCCTTACTATT
TAATCACCAAGGGATTTAGA

Downstream 100 bases:

>100_bases
CTATTTGCAGCGTTTTTTCGTACAATTCGCCACCGACCTGTCCCTATAATTTTAACTATAAGAGCACCAGTATCATGGAC
TCCATGCTGCTTTATATTAT

Product: vitamin B12 binding protein

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 289; Mature: 289

Protein sequence:

>289_residues
MKPSALVTSLTILFSSLWLPLSFAEETLLTSEKVQPAQRVVSLAPHATELAYSAGLGDKLVAVSERSDYPLQADKLEKVA
NYQGIKVEKIIALQPDLILAWPAGNPPRELAKLEQFGFNIYYSKTKSLDNIATNIEQLSQFASDPSIGENNAQQYREQLN
ALRLKYKDAEPVNYFYQLSEKPIITVAQGHWPSEVFEFCGGHNIFEDSASPYPQVGIEQVVLRKPQVIFTSQHAIENGTM
WQTWEDEIPAVAQNQIWSLNSDWINRPTTRTLKAIQQVCDFFDRARQNH

Sequences:

>Translated_289_residues
MKPSALVTSLTILFSSLWLPLSFAEETLLTSEKVQPAQRVVSLAPHATELAYSAGLGDKLVAVSERSDYPLQADKLEKVA
NYQGIKVEKIIALQPDLILAWPAGNPPRELAKLEQFGFNIYYSKTKSLDNIATNIEQLSQFASDPSIGENNAQQYREQLN
ALRLKYKDAEPVNYFYQLSEKPIITVAQGHWPSEVFEFCGGHNIFEDSASPYPQVGIEQVVLRKPQVIFTSQHAIENGTM
WQTWEDEIPAVAQNQIWSLNSDWINRPTTRTLKAIQQVCDFFDRARQNH
>Mature_289_residues
MKPSALVTSLTILFSSLWLPLSFAEETLLTSEKVQPAQRVVSLAPHATELAYSAGLGDKLVAVSERSDYPLQADKLEKVA
NYQGIKVEKIIALQPDLILAWPAGNPPRELAKLEQFGFNIYYSKTKSLDNIATNIEQLSQFASDPSIGENNAQQYREQLN
ALRLKYKDAEPVNYFYQLSEKPIITVAQGHWPSEVFEFCGGHNIFEDSASPYPQVGIEQVVLRKPQVIFTSQHAIENGTM
WQTWEDEIPAVAQNQIWSLNSDWINRPTTRTLKAIQQVCDFFDRARQNH

Specific function: Part of the ABC transporter complex BtuCDF involved in vitamin B12 import. Binds vitamin B12 and delivers it to the periplasmic surface of BtuC [H]

COG id: COG0614

COG function: function code P; ABC-type Fe3+-hydroxamate transport system, periplasmic component

Gene ontology:

Cell location: Periplasm [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 Fe/B12 periplasmic-binding domain [H]

Homologues:

Organism=Escherichia coli, GI1786353, Length=270, Percent_Identity=36.2962962962963, Blast_Score=167, Evalue=7e-43,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR002491 [H]

Pfam domain/function: PF01497 Peripla_BP_2 [H]

EC number: NA

Molecular weight: Translated: 32668; Mature: 32668

Theoretical pI: Translated: 5.02; Mature: 5.02

Prosite motif: PS50983 FE_B12_PBP

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.7 %Cys     (Translated Protein)
0.7 %Met     (Translated Protein)
1.4 %Cys+Met (Translated Protein)
0.7 %Cys     (Mature Protein)
0.7 %Met     (Mature Protein)
1.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKPSALVTSLTILFSSLWLPLSFAEETLLTSEKVQPAQRVVSLAPHATELAYSAGLGDKL
CCCHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHCCHHHHHHHHCCCHHHHHHHCCCCCCE
VAVSERSDYPLQADKLEKVANYQGIKVEKIIALQPDLILAWPAGNPPRELAKLEQFGFNI
EEEECCCCCCCCHHHHHHHHCCCCCEEEEEEEECCCEEEEECCCCCHHHHHHHHHHCCEE
YYSKTKSLDNIATNIEQLSQFASDPSIGENNAQQYREQLNALRLKYKDAEPVNYFYQLSE
EEECCCCHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHCCCCCHHHHHHHCCC
KPIITVAQGHWPSEVFEFCGGHNIFEDSASPYPQVGIEQVVLRKPQVIFTSQHAIENGTM
CCEEEEECCCCHHHHHHHHCCCCCCCCCCCCCCCCCHHHHHHCCCCEEEECCHHHCCCCC
WQTWEDEIPAVAQNQIWSLNSDWINRPTTRTLKAIQQVCDFFDRARQNH
CCCHHHCCCHHHHCCCCCCCCHHCCCCHHHHHHHHHHHHHHHHHHHCCC
>Mature Secondary Structure
MKPSALVTSLTILFSSLWLPLSFAEETLLTSEKVQPAQRVVSLAPHATELAYSAGLGDKL
CCCHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHCCHHHHHHHHCCCHHHHHHHCCCCCCE
VAVSERSDYPLQADKLEKVANYQGIKVEKIIALQPDLILAWPAGNPPRELAKLEQFGFNI
EEEECCCCCCCCHHHHHHHHCCCCCEEEEEEEECCCEEEEECCCCCHHHHHHHHHHCCEE
YYSKTKSLDNIATNIEQLSQFASDPSIGENNAQQYREQLNALRLKYKDAEPVNYFYQLSE
EEECCCCHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHCCCCCHHHHHHHCCC
KPIITVAQGHWPSEVFEFCGGHNIFEDSASPYPQVGIEQVVLRKPQVIFTSQHAIENGTM
CCEEEEECCCCHHHHHHHHCCCCCCCCCCCCCCCCCHHHHHHCCCCEEEECCHHHCCCCC
WQTWEDEIPAVAQNQIWSLNSDWINRPTTRTLKAIQQVCDFFDRARQNH
CCCHHHCCCHHHHCCCCCCCCHHCCCCHHHHHHHHHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA