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The map label for this gene is ksgA

Identifier: 218708408

GI number: 218708408

Start: 368082

End: 368897

Strand: Reverse

Name: ksgA

Synonym: VS_0370

Alternate gene names: 218708408

Gene position: 368897-368082 (Counterclockwise)

Preceding gene: 218708409

Following gene: 218708407

Centisome position: 11.18

GC content: 45.22

Gene sequence:

>816_bases
ATGAGAAATGATGTCCACTTAGGGCACAAAGCGCGTAAACGTTTTGGTCAAAACTTCCTTAACGACCCATACATTATTGA
TGGAATCGTATCGAGTATTAACCCACTACCAGGCCAGAACTTAGTAGAAATCGGCCCCGGTCTTGGTGCGATTACTGAGC
CTGTTGGCAAGCTTGTCGACAAGTTCACAGTAATTGAACTGGATAGAGATCTTGCTGAACGTCTGCGTAATCACCCTGAT
CTTGCTGAAAAGTTAACGATCTATGAAGGCGATGCAATGAAGTTCGATTTCGAACAACTTGTTAAGCCAAACAACAAGCT
ACGTATCTTCGGTAACTTGCCATACAACATCTCTACACCATTGATGTTCCACCTTTTTGAGTTCCATAAAGACGTGCAAG
ACATGCACTTTATGCTTCAAAAAGAAGTCGTTAACCGATTAGCAGCGGGTCCTGGTACTAAAGCTTACGGTCGTCTAACG
GTTATGGCTCAATATTACTGTAAAGTCACTCCTGTGTTAGAAGTGCCACCAACAGCATTTGTTCCGCCACCGAAAGTAGA
CTCTGCAGTCGTTCGCCTTCAGCCTTATGAAGTACTGCCATACCCGGCAAAAGATCTTAAGTGGTTAGATCGCGTATGTC
GCGAAGGCTTTAACCAGCGTCGTAAAACAGTACGTAACTGCTACAAGAGCCTAATCGACAAGGAAGTGCTTGAAGAGCTA
GGTGTAAACCCTGGCATGCGCCCTGAGAACCTGACGCTCGAACAGTTTGTCGATATGGCGAACTGGTTGCACGACAGTCA
CAACGCTGACAAATAA

Upstream 100 bases:

>100_bases
ATCACGGTACCGCCTTAGACTTGGCAGGGAAAGGCCAAGCCGATACAGGGAGCTTTAGAACAGCGCTCACGCACGCCATT
GAATTAGTAGAGAAAAAACA

Downstream 100 bases:

>100_bases
TCGAAAAAGGCTCCTACACTTCGGTGAGGAGCCTATTTTTTATGTAATACTTAAAGCATACCAACAACAAAAGGTGCGAA
TATGGATATATCTACGCCTT

Product: dimethyladenosine transferase

Products: NA

Alternate protein names: 16S rRNA (adenine(1518)-N(6)/adenine(1519)-N(6))-dimethyltransferase; 16S rRNA dimethyladenosine transferase; 16S rRNA dimethylase; S-adenosylmethionine-6-N', N'-adenosyl(rRNA) dimethyltransferase

Number of amino acids: Translated: 271; Mature: 271

Protein sequence:

>271_residues
MRNDVHLGHKARKRFGQNFLNDPYIIDGIVSSINPLPGQNLVEIGPGLGAITEPVGKLVDKFTVIELDRDLAERLRNHPD
LAEKLTIYEGDAMKFDFEQLVKPNNKLRIFGNLPYNISTPLMFHLFEFHKDVQDMHFMLQKEVVNRLAAGPGTKAYGRLT
VMAQYYCKVTPVLEVPPTAFVPPPKVDSAVVRLQPYEVLPYPAKDLKWLDRVCREGFNQRRKTVRNCYKSLIDKEVLEEL
GVNPGMRPENLTLEQFVDMANWLHDSHNADK

Sequences:

>Translated_271_residues
MRNDVHLGHKARKRFGQNFLNDPYIIDGIVSSINPLPGQNLVEIGPGLGAITEPVGKLVDKFTVIELDRDLAERLRNHPD
LAEKLTIYEGDAMKFDFEQLVKPNNKLRIFGNLPYNISTPLMFHLFEFHKDVQDMHFMLQKEVVNRLAAGPGTKAYGRLT
VMAQYYCKVTPVLEVPPTAFVPPPKVDSAVVRLQPYEVLPYPAKDLKWLDRVCREGFNQRRKTVRNCYKSLIDKEVLEEL
GVNPGMRPENLTLEQFVDMANWLHDSHNADK
>Mature_271_residues
MRNDVHLGHKARKRFGQNFLNDPYIIDGIVSSINPLPGQNLVEIGPGLGAITEPVGKLVDKFTVIELDRDLAERLRNHPD
LAEKLTIYEGDAMKFDFEQLVKPNNKLRIFGNLPYNISTPLMFHLFEFHKDVQDMHFMLQKEVVNRLAAGPGTKAYGRLT
VMAQYYCKVTPVLEVPPTAFVPPPKVDSAVVRLQPYEVLPYPAKDLKWLDRVCREGFNQRRKTVRNCYKSLIDKEVLEEL
GVNPGMRPENLTLEQFVDMANWLHDSHNADK

Specific function: Specifically dimethylates two adjacent adenosines (A1518 and A1519) in the loop of a conserved hairpin near the 3'-end of 16S rRNA in the 30S particle. May play a critical role in biogenesis of 30S subunits

COG id: COG0030

COG function: function code J; Dimethyladenosine transferase (rRNA methylation)

Gene ontology:

Cell location: Cytoplasm (Potential)

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the methyltransferase superfamily. rRNA adenine N(6)-methyltransferase family. RsmA subfamily

Homologues:

Organism=Homo sapiens, GI156415992, Length=279, Percent_Identity=32.6164874551971, Blast_Score=125, Evalue=4e-29,
Organism=Homo sapiens, GI7657198, Length=232, Percent_Identity=32.7586206896552, Blast_Score=112, Evalue=2e-25,
Organism=Escherichia coli, GI1786236, Length=267, Percent_Identity=64.4194756554307, Blast_Score=366, Evalue=1e-103,
Organism=Caenorhabditis elegans, GI25141369, Length=293, Percent_Identity=26.2798634812287, Blast_Score=103, Evalue=7e-23,
Organism=Caenorhabditis elegans, GI25146882, Length=233, Percent_Identity=34.3347639484979, Blast_Score=102, Evalue=2e-22,
Organism=Saccharomyces cerevisiae, GI6324989, Length=229, Percent_Identity=32.3144104803493, Blast_Score=107, Evalue=2e-24,
Organism=Drosophila melanogaster, GI21357273, Length=277, Percent_Identity=31.7689530685921, Blast_Score=114, Evalue=9e-26,
Organism=Drosophila melanogaster, GI21358017, Length=243, Percent_Identity=33.3333333333333, Blast_Score=106, Evalue=2e-23,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): RSMA_VIBSL (B7VIE2)

Other databases:

- EMBL:   FM954972
- RefSeq:   YP_002416029.1
- ProteinModelPortal:   B7VIE2
- SMR:   B7VIE2
- GeneID:   7160007
- GenomeReviews:   FM954972_GR
- KEGG:   vsp:VS_0370
- HOGENOM:   HBG319664
- OMA:   RAENLTP
- ProtClustDB:   PRK00274
- GO:   GO:0005737
- HAMAP:   MF_00607
- InterPro:   IPR023165
- InterPro:   IPR020596
- InterPro:   IPR001737
- InterPro:   IPR020598
- InterPro:   IPR011530
- Gene3D:   G3DSA:1.10.8.100
- PANTHER:   PTHR11727
- SMART:   SM00650
- TIGRFAMs:   TIGR00755

Pfam domain/function: PF00398 RrnaAD

EC number: =2.1.1.182

Molecular weight: Translated: 31022; Mature: 31022

Theoretical pI: Translated: 7.66; Mature: 7.66

Prosite motif: PS01131 RRNA_A_DIMETH

Important sites: BINDING 18-18 BINDING 20-20 BINDING 45-45 BINDING 66-66 BINDING 91-91 BINDING 112-112

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.1 %Cys     (Translated Protein)
3.0 %Met     (Translated Protein)
4.1 %Cys+Met (Translated Protein)
1.1 %Cys     (Mature Protein)
3.0 %Met     (Mature Protein)
4.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MRNDVHLGHKARKRFGQNFLNDPYIIDGIVSSINPLPGQNLVEIGPGLGAITEPVGKLVD
CCCCCCCCHHHHHHHHHHHCCCCHHHHHHHHHCCCCCCCCCEECCCCCCHHHHHHHHHHH
KFTVIELDRDLAERLRNHPDLAEKLTIYEGDAMKFDFEQLVKPNNKLRIFGNLPYNISTP
HHHHHHHHHHHHHHHHCCCCHHHHEEEECCCCCEECHHHHCCCCCCEEEEECCCCCCCHH
LMFHLFEFHKDVQDMHFMLQKEVVNRLAAGPGTKAYGRLTVMAQYYCKVTPVLEVPPTAF
HHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHCCCEEECCCCCC
VPPPKVDSAVVRLQPYEVLPYPAKDLKWLDRVCREGFNQRRKTVRNCYKSLIDKEVLEEL
CCCCCCCCCEEEECCCEECCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
GVNPGMRPENLTLEQFVDMANWLHDSHNADK
CCCCCCCCCCCCHHHHHHHHHHHHCCCCCCC
>Mature Secondary Structure
MRNDVHLGHKARKRFGQNFLNDPYIIDGIVSSINPLPGQNLVEIGPGLGAITEPVGKLVD
CCCCCCCCHHHHHHHHHHHCCCCHHHHHHHHHCCCCCCCCCEECCCCCCHHHHHHHHHHH
KFTVIELDRDLAERLRNHPDLAEKLTIYEGDAMKFDFEQLVKPNNKLRIFGNLPYNISTP
HHHHHHHHHHHHHHHHCCCCHHHHEEEECCCCCEECHHHHCCCCCCEEEEECCCCCCCHH
LMFHLFEFHKDVQDMHFMLQKEVVNRLAAGPGTKAYGRLTVMAQYYCKVTPVLEVPPTAF
HHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHCCCEEECCCCCC
VPPPKVDSAVVRLQPYEVLPYPAKDLKWLDRVCREGFNQRRKTVRNCYKSLIDKEVLEEL
CCCCCCCCCEEEECCCEECCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
GVNPGMRPENLTLEQFVDMANWLHDSHNADK
CCCCCCCCCCCCHHHHHHHHHHHHCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA