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The map label for this gene is yqiG [H]

Identifier: 218703577

GI number: 218703577

Start: 357381

End: 358523

Strand: Reverse

Name: yqiG [H]

Synonym: ECUMN_0331

Alternate gene names: 218703577

Gene position: 358523-357381 (Counterclockwise)

Preceding gene: 218703578

Following gene: 218703576

Centisome position: 6.89

GC content: 44.01

Gene sequence:

>1143_bases
ATGACAAATAAACACCCATCATTGTTTTCTCCTTTTATGCTGACAGAAAAAATAAAGCTACGTAATCGTATCGTTATGGC
TCCTATGACGACCTGGTCAGCGAATCCTGATGGAACCATCTCTGAGCAAGAACTCGAATTTTATAAACGTCGCTCACAAA
ATGTTGGTTTAGTAATAACAGGATGTACCTATGTCACGCCAAGCGGCATTGGGTTTACTCATGAATTTGCCGCGTATGAC
GACCGTTTCATTAACAGCCTTGAAAAGTTAGCAGCGGCAGCCAAAAGTGGCGGTGCTCCGGCTATTTTGCAGATTTTCCA
CGCCGGTAATAAAGCCATCCCGGAGCTTGTACCAAATAACGATGTTATTAGTGCCAGCGCATCAAGCGTAAAATCTGGTG
ACTTTATGAAGAGAGTGGTACAAAGCAGGGAAATGACTGAAAACGAAATTCAGGAAACCATTAGAGCATTCGGAGATGTC
ACAAAAAGGGCGATTAAAGCAGGTTTTGATGGCATTGAACTTCATGGTGCGCACGGATTCCTTCTACAGAATTTCTTCTC
CCCTCTATTTAACCAGCGTAATGACCGATGGGGAGGAGATCTCGAAAGACGTATGCGATTCCCGCTGGCCGTATTACAGG
AAGTAAAAAATGTAGTCTATGAATATGCGACAAAGCCCTTTGCCATCGGTTACAGAATATCTCCTGAAGAATCTGCAACA
GGGGGGCTGCGGATAGAGGATACTTATAAACTCCTTGATCGGCTCATTGCATCCGGTATTTCGTATATACATACCTCGCT
GGTCAGTATTAACGATAGCTACCCTGTTGAGTCGCCAAATGGCCCCCGAACTATTGAATTGATTTTAAATCATATTGCAG
GCAGAGTGCCGGTAATCGCTGCAGGTAAAATACGCACACCGTCTCAGGCTCAAGAAGCAATTTCCACTGGCCTGCCACTT
GTTGCGATCGGTAAAGGGCTGGTGATAAATCCAGAATGGGTGACTTTAGCTGAATCAGGTAGAAGCCATGAAATTCAAAC
AACCCTTAATCCACAACTTGTTCCTGAATTAACCATTCCGGATAAACTATGGGATCAAATTCAGGCATCGAAAGGAACTG
GCTGGTTTCCGTTAATGGATTAA

Upstream 100 bases:

>100_bases
GGAATTATAAATAAAGCCATAAACCTTGGTTCCAAAGCTGGCAACAACTAACCAGTTAAAACATACGCATCAAAATCAGA
TAAACATTTGAGGTATTGTT

Downstream 100 bases:

>100_bases
ATATCTATTACTCTCCCGAGTGTAAAACTATCAAACCAATGAGTAACCGATACAGCCTCGCAAATTCTTTCAGATAATTA
ACCCGTCACTGCGGCACGTT

Product: putative NADH-dependent flavin oxidoreductase

Products: trans-transtetradehydroacyl-CoA; NADPH [C]

Alternate protein names: NA

Number of amino acids: Translated: 380; Mature: 379

Protein sequence:

>380_residues
MTNKHPSLFSPFMLTEKIKLRNRIVMAPMTTWSANPDGTISEQELEFYKRRSQNVGLVITGCTYVTPSGIGFTHEFAAYD
DRFINSLEKLAAAAKSGGAPAILQIFHAGNKAIPELVPNNDVISASASSVKSGDFMKRVVQSREMTENEIQETIRAFGDV
TKRAIKAGFDGIELHGAHGFLLQNFFSPLFNQRNDRWGGDLERRMRFPLAVLQEVKNVVYEYATKPFAIGYRISPEESAT
GGLRIEDTYKLLDRLIASGISYIHTSLVSINDSYPVESPNGPRTIELILNHIAGRVPVIAAGKIRTPSQAQEAISTGLPL
VAIGKGLVINPEWVTLAESGRSHEIQTTLNPQLVPELTIPDKLWDQIQASKGTGWFPLMD

Sequences:

>Translated_380_residues
MTNKHPSLFSPFMLTEKIKLRNRIVMAPMTTWSANPDGTISEQELEFYKRRSQNVGLVITGCTYVTPSGIGFTHEFAAYD
DRFINSLEKLAAAAKSGGAPAILQIFHAGNKAIPELVPNNDVISASASSVKSGDFMKRVVQSREMTENEIQETIRAFGDV
TKRAIKAGFDGIELHGAHGFLLQNFFSPLFNQRNDRWGGDLERRMRFPLAVLQEVKNVVYEYATKPFAIGYRISPEESAT
GGLRIEDTYKLLDRLIASGISYIHTSLVSINDSYPVESPNGPRTIELILNHIAGRVPVIAAGKIRTPSQAQEAISTGLPL
VAIGKGLVINPEWVTLAESGRSHEIQTTLNPQLVPELTIPDKLWDQIQASKGTGWFPLMD
>Mature_379_residues
TNKHPSLFSPFMLTEKIKLRNRIVMAPMTTWSANPDGTISEQELEFYKRRSQNVGLVITGCTYVTPSGIGFTHEFAAYDD
RFINSLEKLAAAAKSGGAPAILQIFHAGNKAIPELVPNNDVISASASSVKSGDFMKRVVQSREMTENEIQETIRAFGDVT
KRAIKAGFDGIELHGAHGFLLQNFFSPLFNQRNDRWGGDLERRMRFPLAVLQEVKNVVYEYATKPFAIGYRISPEESATG
GLRIEDTYKLLDRLIASGISYIHTSLVSINDSYPVESPNGPRTIELILNHIAGRVPVIAAGKIRTPSQAQEAISTGLPLV
AIGKGLVINPEWVTLAESGRSHEIQTTLNPQLVPELTIPDKLWDQIQASKGTGWFPLMD

Specific function: Catalyzes The NADP-Dependent Reduction Of 2,4-Dienoyl- CoA To Yield Trans-2- Enoyl-CoA. [C]

COG id: COG1902

COG function: function code C; NADH:flavin oxidoreductases, Old Yellow Enzyme family

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the NADH:flavin oxidoreductase/NADH oxidase family [H]

Homologues:

Organism=Escherichia coli, GI1789463, Length=352, Percent_Identity=28.6931818181818, Blast_Score=123, Evalue=2e-29,
Organism=Escherichia coli, GI1787939, Length=360, Percent_Identity=28.3333333333333, Blast_Score=108, Evalue=5e-25,
Organism=Caenorhabditis elegans, GI17559804, Length=278, Percent_Identity=28.0575539568345, Blast_Score=95, Evalue=8e-20,
Organism=Caenorhabditis elegans, GI17564188, Length=375, Percent_Identity=25.8666666666667, Blast_Score=94, Evalue=2e-19,
Organism=Caenorhabditis elegans, GI17540738, Length=260, Percent_Identity=28.4615384615385, Blast_Score=92, Evalue=3e-19,
Organism=Caenorhabditis elegans, GI72001454, Length=277, Percent_Identity=27.0758122743682, Blast_Score=87, Evalue=1e-17,
Organism=Caenorhabditis elegans, GI17566914, Length=272, Percent_Identity=27.2058823529412, Blast_Score=84, Evalue=9e-17,
Organism=Caenorhabditis elegans, GI17559802, Length=221, Percent_Identity=30.7692307692308, Blast_Score=81, Evalue=1e-15,
Organism=Caenorhabditis elegans, GI17565138, Length=185, Percent_Identity=29.7297297297297, Blast_Score=78, Evalue=6e-15,
Organism=Saccharomyces cerevisiae, GI6321973, Length=355, Percent_Identity=25.6338028169014, Blast_Score=107, Evalue=3e-24,
Organism=Saccharomyces cerevisiae, GI6325086, Length=358, Percent_Identity=24.5810055865922, Blast_Score=105, Evalue=2e-23,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR013785
- InterPro:   IPR001155 [H]

Pfam domain/function: PF00724 Oxidored_FMN [H]

EC number: 1.3.1.34 [C]

Molecular weight: Translated: 41938; Mature: 41807

Theoretical pI: Translated: 7.30; Mature: 7.30

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.3 %Cys     (Translated Protein)
2.1 %Met     (Translated Protein)
2.4 %Cys+Met (Translated Protein)
0.3 %Cys     (Mature Protein)
1.8 %Met     (Mature Protein)
2.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTNKHPSLFSPFMLTEKIKLRNRIVMAPMTTWSANPDGTISEQELEFYKRRSQNVGLVIT
CCCCCCCCCCHHHHHHHHHHCCEEEEEEECCCCCCCCCCCCHHHHHHHHHHCCCCCEEEE
GCTYVTPSGIGFTHEFAAYDDRFINSLEKLAAAAKSGGAPAILQIFHAGNKAIPELVPNN
CCEEECCCCCCCCHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHCCCCCCCCCCCCC
DVISASASSVKSGDFMKRVVQSREMTENEIQETIRAFGDVTKRAIKAGFDGIELHGAHGF
CEEECCHHHCCCHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHCCCCEEEECCCHH
LLQNFFSPLFNQRNDRWGGDLERRMRFPLAVLQEVKNVVYEYATKPFAIGYRISPEESAT
HHHHHHHHHHCCCCCCCCCCHHHHHHCHHHHHHHHHHHHHHHCCCCEEEEEEECCCCCCC
GGLRIEDTYKLLDRLIASGISYIHTSLVSINDSYPVESPNGPRTIELILNHIAGRVPVIA
CCCEEHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCHHHHHHHHHHCCCCCEEE
AGKIRTPSQAQEAISTGLPLVAIGKGLVINPEWVTLAESGRSHEIQTTLNPQLVPELTIP
ECCCCCCHHHHHHHHCCCCEEEECCCEEECCCEEEEECCCCCCEEEECCCCCCCCCCCCC
DKLWDQIQASKGTGWFPLMD
HHHHHHHHCCCCCCCCCCCC
>Mature Secondary Structure 
TNKHPSLFSPFMLTEKIKLRNRIVMAPMTTWSANPDGTISEQELEFYKRRSQNVGLVIT
CCCCCCCCCHHHHHHHHHHCCEEEEEEECCCCCCCCCCCCHHHHHHHHHHCCCCCEEEE
GCTYVTPSGIGFTHEFAAYDDRFINSLEKLAAAAKSGGAPAILQIFHAGNKAIPELVPNN
CCEEECCCCCCCCHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHCCCCCCCCCCCCC
DVISASASSVKSGDFMKRVVQSREMTENEIQETIRAFGDVTKRAIKAGFDGIELHGAHGF
CEEECCHHHCCCHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHCCCCEEEECCCHH
LLQNFFSPLFNQRNDRWGGDLERRMRFPLAVLQEVKNVVYEYATKPFAIGYRISPEESAT
HHHHHHHHHHCCCCCCCCCCHHHHHHCHHHHHHHHHHHHHHHCCCCEEEEEEECCCCCCC
GGLRIEDTYKLLDRLIASGISYIHTSLVSINDSYPVESPNGPRTIELILNHIAGRVPVIA
CCCEEHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCHHHHHHHHHHCCCCCEEE
AGKIRTPSQAQEAISTGLPLVAIGKGLVINPEWVTLAESGRSHEIQTTLNPQLVPELTIP
ECCCCCCHHHHHHHHCCCCEEEECCCEEECCCEEEEECCCCCCEEEECCCCCCCCCCCCC
DKLWDQIQASKGTGWFPLMD
HHHHHHHHCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: FAD. [C]

Metal ions: Fe [C]

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: transDidehydroacyl-CoA; NADP+ [C]

Specific reaction: transDidehydroacyl-CoA + NADP+ =trans-transtetradehydroacyl-CoA + NADPH [C]

General reaction: Redox reaction [C]

Inhibitor: Iodoacetic acid; N-Ethyl maleimide [C]

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 8969508; 9384377 [H]