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The map label for this gene is cytR

Identifier: 218701361

GI number: 218701361

Start: 3188459

End: 3189484

Strand: Direct

Name: cytR

Synonym: ECIAI39_3060

Alternate gene names: 218701361

Gene position: 3188459-3189484 (Clockwise)

Preceding gene: 218701360

Following gene: 218701362

Centisome position: 62.13

GC content: 51.66

Gene sequence:

>1026_bases
GTGAAAGCGAAGAAGCAGGAAACTGCCGCGACCATGAAAGACGTTGCCCTCAAGGCAAAAGTCTCTACAGCGACTGTCTC
CCGAGCATTAATGAATCCCGATAAAGTCTCCCAGGCCACCCGTAATCGGGTTGAAAAAGCGGCCCGGGAAGTGGGTTATT
TACCGCAGCCTATGGGGCGCAACGTCAAGCGTAATGAATCCCGTACCATTCTGGTGATTGTCCCGGATATCTGCGATCCC
TTCTTTAGCGAAATTATTCGCGGTATCGAAGTTACGGCGGCAAATCACGGATATCTGGTGCTGATTGGCGACTGTGCGCA
TCAAAATCAGCAGGAAAAAACCTTTATCGATTTGATCATCACCAAGCAAATTGATGGCATGTTGCTGCTGGGTTCAAGGC
TGCCGTTTGATGCCAGCATTGAGGAACAGCGTAATCTGCCGCCGATGGTGATGGCGAACGAATTTGCACCGGAACTGGAG
CTACCTACCGTTCATATCGACAATCTGACCGCCGCATTTGATGCAGTAAATTATTTATATGAGCAAGGGCATAAACGGAT
TGGCTGTATAGCCGGTCCCGAAGAGATGCCGCTGTGTCACTACCGCCTGCAAGGTTACGTTCAGGCGCTGCGTCGCTGCG
GCATTATGGTTGATCCGCAATACATCGCCCGTGGCGACTTCACCTTCGAAGCCGGAAGCAAAGCAATGCAACAGCTGCTT
GATCTTCCACAACCGCCTACTGCTGTCTTCTGCCATAGCGATGTGATGGCGCTCGGCGCACTTTCTCAGGCAAAACGTCA
GGGGCTGAAAGTCCCGGAAGACCTTTCCATAATCGGTTTTGATAACATCGACCTGACGCAATTTTGTGATCCGCCGCTGA
CAACCATCGCGCAGCCGCGTTACGAAATCGGTCGGGAAGCTATGCTGTTATTGCTTGATCAAATGCAGGGGCAGCACGTT
GGCAGTGGCTCTCGTTTAATGGACTGCGAACTTATCATCCGGGGATCAACACGCGCGTTACCTTAA

Upstream 100 bases:

>100_bases
AAAATTCTGTAACCGTTTTCACGCGCTATCTGCTAAAAATGTTGCCGATGTGAAGTAAACATGGAAGTAGTACGCCTGCC
GTGCCAGGCGAGGAGTGAGT

Downstream 100 bases:

>100_bases
AGTAAACCTTTAAGACTGCCGTGACTGGTCAAAGGCCCGCCGCTTAAGTAACATGGCGGGCTGACGAACGAATAAATACA
GCGAAACGATAGTGGCACAA

Product: DNA-binding transcriptional regulator CytR

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 341; Mature: 341

Protein sequence:

>341_residues
MKAKKQETAATMKDVALKAKVSTATVSRALMNPDKVSQATRNRVEKAAREVGYLPQPMGRNVKRNESRTILVIVPDICDP
FFSEIIRGIEVTAANHGYLVLIGDCAHQNQQEKTFIDLIITKQIDGMLLLGSRLPFDASIEEQRNLPPMVMANEFAPELE
LPTVHIDNLTAAFDAVNYLYEQGHKRIGCIAGPEEMPLCHYRLQGYVQALRRCGIMVDPQYIARGDFTFEAGSKAMQQLL
DLPQPPTAVFCHSDVMALGALSQAKRQGLKVPEDLSIIGFDNIDLTQFCDPPLTTIAQPRYEIGREAMLLLLDQMQGQHV
GSGSRLMDCELIIRGSTRALP

Sequences:

>Translated_341_residues
MKAKKQETAATMKDVALKAKVSTATVSRALMNPDKVSQATRNRVEKAAREVGYLPQPMGRNVKRNESRTILVIVPDICDP
FFSEIIRGIEVTAANHGYLVLIGDCAHQNQQEKTFIDLIITKQIDGMLLLGSRLPFDASIEEQRNLPPMVMANEFAPELE
LPTVHIDNLTAAFDAVNYLYEQGHKRIGCIAGPEEMPLCHYRLQGYVQALRRCGIMVDPQYIARGDFTFEAGSKAMQQLL
DLPQPPTAVFCHSDVMALGALSQAKRQGLKVPEDLSIIGFDNIDLTQFCDPPLTTIAQPRYEIGREAMLLLLDQMQGQHV
GSGSRLMDCELIIRGSTRALP
>Mature_341_residues
MKAKKQETAATMKDVALKAKVSTATVSRALMNPDKVSQATRNRVEKAAREVGYLPQPMGRNVKRNESRTILVIVPDICDP
FFSEIIRGIEVTAANHGYLVLIGDCAHQNQQEKTFIDLIITKQIDGMLLLGSRLPFDASIEEQRNLPPMVMANEFAPELE
LPTVHIDNLTAAFDAVNYLYEQGHKRIGCIAGPEEMPLCHYRLQGYVQALRRCGIMVDPQYIARGDFTFEAGSKAMQQLL
DLPQPPTAVFCHSDVMALGALSQAKRQGLKVPEDLSIIGFDNIDLTQFCDPPLTTIAQPRYEIGREAMLLLLDQMQGQHV
GSGSRLMDCELIIRGSTRALP

Specific function: This protein negatively controls the transcription initiation of genes such as deoCABD, udp, and cdd encoding catabolizing enzymes and nupC, nupG, and tsx encoding transporting and pore-forming proteins. Binds cytidine and adenosine as effectors

COG id: COG1609

COG function: function code K; Transcriptional regulators

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 HTH lacI-type DNA-binding domain

Homologues:

Organism=Escherichia coli, GI1790369, Length=341, Percent_Identity=100, Blast_Score=705, Evalue=0.0,
Organism=Escherichia coli, GI1787948, Length=309, Percent_Identity=37.2168284789644, Blast_Score=217, Evalue=1e-57,
Organism=Escherichia coli, GI1790194, Length=309, Percent_Identity=35.5987055016181, Blast_Score=200, Evalue=1e-52,
Organism=Escherichia coli, GI1789202, Length=291, Percent_Identity=33.6769759450172, Blast_Score=176, Evalue=2e-45,
Organism=Escherichia coli, GI1788474, Length=307, Percent_Identity=35.1791530944625, Blast_Score=176, Evalue=2e-45,
Organism=Escherichia coli, GI1789068, Length=311, Percent_Identity=30.5466237942122, Blast_Score=145, Evalue=5e-36,
Organism=Escherichia coli, GI48994940, Length=323, Percent_Identity=29.4117647058824, Blast_Score=138, Evalue=7e-34,
Organism=Escherichia coli, GI1787580, Length=316, Percent_Identity=27.5316455696203, Blast_Score=123, Evalue=2e-29,
Organism=Escherichia coli, GI1790715, Length=313, Percent_Identity=26.517571884984, Blast_Score=119, Evalue=2e-28,
Organism=Escherichia coli, GI1786540, Length=335, Percent_Identity=27.7611940298507, Blast_Score=113, Evalue=2e-26,
Organism=Escherichia coli, GI1787906, Length=316, Percent_Identity=26.2658227848101, Blast_Score=108, Evalue=7e-25,
Organism=Escherichia coli, GI1789456, Length=340, Percent_Identity=27.0588235294118, Blast_Score=96, Evalue=3e-21,
Organism=Escherichia coli, GI1786268, Length=312, Percent_Identity=23.0769230769231, Blast_Score=76, Evalue=4e-15,
Organism=Escherichia coli, GI1790689, Length=315, Percent_Identity=25.0793650793651, Blast_Score=70, Evalue=2e-13,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): CYTR_ECO57 (P0ACN9)

Other databases:

- EMBL:   AE005174
- EMBL:   BA000007
- PIR:   E86083
- PIR:   E91236
- RefSeq:   NP_290565.1
- RefSeq:   NP_312888.1
- ProteinModelPortal:   P0ACN9
- SMR:   P0ACN9
- EnsemblBacteria:   EBESCT00000023892
- EnsemblBacteria:   EBESCT00000057373
- GeneID:   915035
- GeneID:   960203
- GenomeReviews:   AE005174_GR
- GenomeReviews:   BA000007_GR
- KEGG:   ece:Z5481
- KEGG:   ecs:ECs4861
- GeneTree:   EBGT00050000009237
- HOGENOM:   HBG753640
- OMA:   FEAVHYL
- ProtClustDB:   PRK11041
- BioCyc:   ECOL83334:ECS4861-MONOMER
- GO:   GO:0005622
- InterPro:   IPR000843
- InterPro:   IPR010982
- InterPro:   IPR001761
- SMART:   SM00354

Pfam domain/function: PF00356 LacI; PF00532 Peripla_BP_1; SSF47413 Lambda_like_DNA

EC number: NA

Molecular weight: Translated: 37820; Mature: 37820

Theoretical pI: Translated: 6.40; Mature: 6.40

Prosite motif: PS00356 HTH_LACI_1; PS50932 HTH_LACI_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.3 %Cys     (Translated Protein)
4.1 %Met     (Translated Protein)
6.5 %Cys+Met (Translated Protein)
2.3 %Cys     (Mature Protein)
4.1 %Met     (Mature Protein)
6.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKAKKQETAATMKDVALKAKVSTATVSRALMNPDKVSQATRNRVEKAAREVGYLPQPMGR
CCCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHCCCCCCCCC
NVKRNESRTILVIVPDICDPFFSEIIRGIEVTAANHGYLVLIGDCAHQNQQEKTFIDLII
CCCCCCCCEEEEEECCCCCHHHHHHHCCEEEEECCCCEEEEEECCCCCCCCHHHHEEEEE
TKQIDGMLLLGSRLPFDASIEEQRNLPPMVMANEFAPELELPTVHIDNLTAAFDAVNYLY
ECCCCCEEEECCCCCCCCCCHHHCCCCCEEEECCCCCCCCCCEEEECCHHHHHHHHHHHH
EQGHKRIGCIAGPEEMPLCHYRLQGYVQALRRCGIMVDPQYIARGDFTFEAGSKAMQQLL
HCCCCEEEEECCCCCCCHHHHHHHHHHHHHHHCCCEECCHHHHCCCCEEHHHHHHHHHHH
DLPQPPTAVFCHSDVMALGALSQAKRQGLKVPEDLSIIGFDNIDLTQFCDPPLTTIAQPR
CCCCCCCEEEECCHHHHHHHHHHHHHCCCCCCCCCEEEECCCCCHHHHCCCCHHHHCCCH
YEIGREAMLLLLDQMQGQHVGSGSRLMDCELIIRGSTRALP
HHHHHHHHHHHHHHHCCCCCCCCCCEEEEEEEEECCCCCCC
>Mature Secondary Structure
MKAKKQETAATMKDVALKAKVSTATVSRALMNPDKVSQATRNRVEKAAREVGYLPQPMGR
CCCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHCCCCCCCCC
NVKRNESRTILVIVPDICDPFFSEIIRGIEVTAANHGYLVLIGDCAHQNQQEKTFIDLII
CCCCCCCCEEEEEECCCCCHHHHHHHCCEEEEECCCCEEEEEECCCCCCCCHHHHEEEEE
TKQIDGMLLLGSRLPFDASIEEQRNLPPMVMANEFAPELELPTVHIDNLTAAFDAVNYLY
ECCCCCEEEECCCCCCCCCCHHHCCCCCEEEECCCCCCCCCCEEEECCHHHHHHHHHHHH
EQGHKRIGCIAGPEEMPLCHYRLQGYVQALRRCGIMVDPQYIARGDFTFEAGSKAMQQLL
HCCCCEEEEECCCCCCCHHHHHHHHHHHHHHHCCCEECCHHHHCCCCEEHHHHHHHHHHH
DLPQPPTAVFCHSDVMALGALSQAKRQGLKVPEDLSIIGFDNIDLTQFCDPPLTTIAQPR
CCCCCCCEEEECCHHHHHHHHHHHHHCCCCCCCCCEEEECCCCCHHHHCCCCHHHHCCCH
YEIGREAMLLLLDQMQGQHVGSGSRLMDCELIIRGSTRALP
HHHHHHHHHHHHHHHCCCCCCCCCCEEEEEEEEECCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: DNA [C]

Specific reaction: Protein + DNA = Protein-DNA [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 11206551; 11258796