Click here to switch to the map view.

The map label for this gene is hyfF

Identifier: 218700944

GI number: 218700944

Start: 2723641

End: 2725221

Strand: Direct

Name: hyfF

Synonym: ECIAI39_2625

Alternate gene names: 218700944

Gene position: 2723641-2725221 (Clockwise)

Preceding gene: 218700943

Following gene: 218700945

Centisome position: 53.07

GC content: 54.21

Gene sequence:

>1581_bases
ATGAGTTATTCTGTGATGTTCGCTTTACTCCTGCTCACGCCGCTGCTTTTTTCGCTGCTCTGTTTTGCCTGCCGGAAACG
GGGGCACTCTGCGACTTGCGCGGTGACAGTATTACATAGCTTAGGGATCACACTGCTGCTGATTCTGGCACTCTGGGTGG
TCCAAACTGCCGCTGATGCGGGAGAAATATTCACTGCGGGACTGTGGCTTCATATTGATGGTCTGGGCGGTTTGTTCCTC
GCCATTCTTGGTGTGATTGGCTTTCTCACCGGTGTTTACTCGATTGGCTACATGCGTCATGAAGTGGAGCACGGCGAGCT
TTCACCCGTTACGCTGTGCGATTACTACGGTTTCTTCCATCTGTTTTTGTTCACCATGCTGCTGGTTGTTACCAGCAATA
ACCTGATTGTGATGTGGGCGGCGATCAAAGCCACCACCTTAAGCTCGGCGTTTCTGGTAGGCATTTACGGTCAGCGTTCA
TCGCTGGAAGCTGCATGGAAGTACATCATTATTTGTACTGTTGGTGTCGCTTTTGGTCTGTTCGGTACCGTGCTGGTATA
CGCCAACGCCGCCAGCGTTATGCCGCAGGCAGAAATGGCGATATTCTGGAGCGAGGTTCTTAAGCAATCGTCCTTGCTTG
ACCCAACATTAATGCTGTTGGCCTTTGTGTTTGTGCTAATTGGCTTTGGCACTAAAACCGGGCTATTCCCCATGCACGCC
TGGCTGTCAGATGCTCACAGTGAAGCGCCGAGTCCGGTCAGCGCCCTGCTCTCCGCCGTATTGCTGAACTGCGCGCTATT
GGTGCTGATTCGCTATTACATCATTATTTGCCAGGCCATCGGCAGCGATTTCCCCAACCGGTTGTTGCTCATCTTCGGCA
TGTTGTCGGTTGCCGTGGCGGCATTTTTCATTCTGGTACAGCGGGACATTAAGCGTCTGCTGGCGTACTCCAGCGTGGAG
AACATGGGACTGGTCGCGGTGGCGCTAGGCATTGGCGGGCCGCTGGGAATTTTTGCTGCGCTGCTGCACACCTTAAACCA
CAGTCTGGCAAAAACGCTGCTGTTCTGCGGTTCCGGCAATGTACTGCTCAAGTACGGCACGCGCGATCTCAACGTCGTCT
GCGGAATGCTCAAAATCATGCCATTTACCGCCGTGCTGTTTGGCGGCGGTGCGCTGGCGCTGGCCGGGATGCCGCCCTTC
AACATTTTTCTTAGCGAATTTATGACCGTTACCGCCGGACTGGCGCGTAATCACCTGCTGATTATCGTCCTGCTGTTATT
GCTGTTAACGCTGGTGCTGGCGGGCCTGGTACGGATGGCTGCGCGGGTGTTAATGGCGAAACCGCCGCAGGCCGTTAACC
GGGGTGAACTTGGCTGGTTGACCACCTCGCCAATGGTGATTCTGCTGGTCATGATGCTGGCGATGGGAACGCATATTCCA
CAACCTGTCATCAGGATCCTGGCGGGCGCTTCCACTATAGTCCTCTCAGGGACGCACGACCTGCCTGCACAACGTAGCAC
CTGGCATGATTTTTTGCCTTCAGGCACCGCATCTGTTTCGGAGAAACACAGTGAACGTTAA

Upstream 100 bases:

>100_bases
ATCTTCGCCGTCATTGTGATGGTGTTACTGGCAAGAAAAATATGGCGTACCCACGGTACGCTGGACGTGAACAACTTGAC
CGCGCTGAAGGGATAATGAG

Downstream 100 bases:

>100_bases
TTCATCGTCAAATCGTGGCGAAGCGATTCTCGCCGCCCTGAAAACGCAGTTCCCCGGCGCGGTGCTGGATGAAGAGCGAC
AAACGCCTGAACAGGTCACC

Product: hydrogenase 4 subunit F

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 526; Mature: 525

Protein sequence:

>526_residues
MSYSVMFALLLLTPLLFSLLCFACRKRGHSATCAVTVLHSLGITLLLILALWVVQTAADAGEIFTAGLWLHIDGLGGLFL
AILGVIGFLTGVYSIGYMRHEVEHGELSPVTLCDYYGFFHLFLFTMLLVVTSNNLIVMWAAIKATTLSSAFLVGIYGQRS
SLEAAWKYIIICTVGVAFGLFGTVLVYANAASVMPQAEMAIFWSEVLKQSSLLDPTLMLLAFVFVLIGFGTKTGLFPMHA
WLSDAHSEAPSPVSALLSAVLLNCALLVLIRYYIIICQAIGSDFPNRLLLIFGMLSVAVAAFFILVQRDIKRLLAYSSVE
NMGLVAVALGIGGPLGIFAALLHTLNHSLAKTLLFCGSGNVLLKYGTRDLNVVCGMLKIMPFTAVLFGGGALALAGMPPF
NIFLSEFMTVTAGLARNHLLIIVLLLLLLTLVLAGLVRMAARVLMAKPPQAVNRGELGWLTTSPMVILLVMMLAMGTHIP
QPVIRILAGASTIVLSGTHDLPAQRSTWHDFLPSGTASVSEKHSER

Sequences:

>Translated_526_residues
MSYSVMFALLLLTPLLFSLLCFACRKRGHSATCAVTVLHSLGITLLLILALWVVQTAADAGEIFTAGLWLHIDGLGGLFL
AILGVIGFLTGVYSIGYMRHEVEHGELSPVTLCDYYGFFHLFLFTMLLVVTSNNLIVMWAAIKATTLSSAFLVGIYGQRS
SLEAAWKYIIICTVGVAFGLFGTVLVYANAASVMPQAEMAIFWSEVLKQSSLLDPTLMLLAFVFVLIGFGTKTGLFPMHA
WLSDAHSEAPSPVSALLSAVLLNCALLVLIRYYIIICQAIGSDFPNRLLLIFGMLSVAVAAFFILVQRDIKRLLAYSSVE
NMGLVAVALGIGGPLGIFAALLHTLNHSLAKTLLFCGSGNVLLKYGTRDLNVVCGMLKIMPFTAVLFGGGALALAGMPPF
NIFLSEFMTVTAGLARNHLLIIVLLLLLLTLVLAGLVRMAARVLMAKPPQAVNRGELGWLTTSPMVILLVMMLAMGTHIP
QPVIRILAGASTIVLSGTHDLPAQRSTWHDFLPSGTASVSEKHSER
>Mature_525_residues
SYSVMFALLLLTPLLFSLLCFACRKRGHSATCAVTVLHSLGITLLLILALWVVQTAADAGEIFTAGLWLHIDGLGGLFLA
ILGVIGFLTGVYSIGYMRHEVEHGELSPVTLCDYYGFFHLFLFTMLLVVTSNNLIVMWAAIKATTLSSAFLVGIYGQRSS
LEAAWKYIIICTVGVAFGLFGTVLVYANAASVMPQAEMAIFWSEVLKQSSLLDPTLMLLAFVFVLIGFGTKTGLFPMHAW
LSDAHSEAPSPVSALLSAVLLNCALLVLIRYYIIICQAIGSDFPNRLLLIFGMLSVAVAAFFILVQRDIKRLLAYSSVEN
MGLVAVALGIGGPLGIFAALLHTLNHSLAKTLLFCGSGNVLLKYGTRDLNVVCGMLKIMPFTAVLFGGGALALAGMPPFN
IFLSEFMTVTAGLARNHLLIIVLLLLLLTLVLAGLVRMAARVLMAKPPQAVNRGELGWLTTSPMVILLVMMLAMGTHIPQ
PVIRILAGASTIVLSGTHDLPAQRSTWHDFLPSGTASVSEKHSER

Specific function: Unknown

COG id: COG0651

COG function: function code CP; Formate hydrogenlyase subunit 3/Multisubunit Na+/H+ antiporter, MnhD subunit

Gene ontology:

Cell location: Cell inner membrane; Multi-pass membrane protein [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the complex I subunit 5 family [H]

Homologues:

Organism=Homo sapiens, GI251831117, Length=412, Percent_Identity=26.4563106796117, Blast_Score=106, Evalue=6e-23,
Organism=Homo sapiens, GI251831116, Length=185, Percent_Identity=30.8108108108108, Blast_Score=71, Evalue=3e-12,
Organism=Escherichia coli, GI1788831, Length=526, Percent_Identity=97.3384030418251, Blast_Score=964, Evalue=0.0,
Organism=Escherichia coli, GI1788827, Length=357, Percent_Identity=29.9719887955182, Blast_Score=141, Evalue=9e-35,
Organism=Escherichia coli, GI1788614, Length=439, Percent_Identity=28.0182232346241, Blast_Score=116, Evalue=4e-27,
Organism=Escherichia coli, GI1788829, Length=412, Percent_Identity=29.126213592233, Blast_Score=115, Evalue=8e-27,
Organism=Escherichia coli, GI1788613, Length=432, Percent_Identity=27.7777777777778, Blast_Score=114, Evalue=2e-26,
Organism=Escherichia coli, GI2367154, Length=194, Percent_Identity=35.5670103092784, Blast_Score=97, Evalue=3e-21,
Organism=Escherichia coli, GI145693160, Length=205, Percent_Identity=32.1951219512195, Blast_Score=80, Evalue=3e-16,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001750 [H]

Pfam domain/function: PF00361 Oxidored_q1 [H]

EC number: 1.-.-.- [C]

Molecular weight: Translated: 56589; Mature: 56458

Theoretical pI: Translated: 8.33; Mature: 8.33

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.7 %Cys     (Translated Protein)
4.0 %Met     (Translated Protein)
5.7 %Cys+Met (Translated Protein)
1.7 %Cys     (Mature Protein)
3.8 %Met     (Mature Protein)
5.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSYSVMFALLLLTPLLFSLLCFACRKRGHSATCAVTVLHSLGITLLLILALWVVQTAADA
CCHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCC
GEIFTAGLWLHIDGLGGLFLAILGVIGFLTGVYSIGYMRHEVEHGELSPVTLCDYYGFFH
CHHHEEEEEEEECCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHH
LFLFTMLLVVTSNNLIVMWAAIKATTLSSAFLVGIYGQRSSLEAAWKYIIICTVGVAFGL
HHHHHHHHHHHCCCEEEEEEHHHHHHHHHHHHEEECCCCHHHHHHHHHHHHHHHHHHHHH
FGTVLVYANAASVMPQAEMAIFWSEVLKQSSLLDPTLMLLAFVFVLIGFGTKTGLFPMHA
HHHHHHHHHHHHHCCHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHCCCCCCCHHHHH
WLSDAHSEAPSPVSALLSAVLLNCALLVLIRYYIIICQAIGSDFPNRLLLIFGMLSVAVA
HHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHH
AFFILVQRDIKRLLAYSSVENMGLVAVALGIGGPLGIFAALLHTLNHSLAKTLLFCGSGN
HHHHHHHHHHHHHHHHHHHCCCCHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHCCCCC
VLLKYGTRDLNVVCGMLKIMPFTAVLFGGGALALAGMPPFNIFLSEFMTVTAGLARNHLL
EEEEECCCHHHHHHHHHHHHHHHHHHHCCCHHEECCCCHHHHHHHHHHHHHHHHHHHHHH
IIVLLLLLLTLVLAGLVRMAARVLMAKPPQAVNRGELGWLTTSPMVILLVMMLAMGTHIP
HHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCEECCCHHHHHHHHHHHHCCCCC
QPVIRILAGASTIVLSGTHDLPAQRSTWHDFLPSGTASVSEKHSER
HHHHHHHHCCCEEEEECCCCCCCCCCHHHHHCCCCCCHHHHHCCCC
>Mature Secondary Structure 
SYSVMFALLLLTPLLFSLLCFACRKRGHSATCAVTVLHSLGITLLLILALWVVQTAADA
CHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCC
GEIFTAGLWLHIDGLGGLFLAILGVIGFLTGVYSIGYMRHEVEHGELSPVTLCDYYGFFH
CHHHEEEEEEEECCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHH
LFLFTMLLVVTSNNLIVMWAAIKATTLSSAFLVGIYGQRSSLEAAWKYIIICTVGVAFGL
HHHHHHHHHHHCCCEEEEEEHHHHHHHHHHHHEEECCCCHHHHHHHHHHHHHHHHHHHHH
FGTVLVYANAASVMPQAEMAIFWSEVLKQSSLLDPTLMLLAFVFVLIGFGTKTGLFPMHA
HHHHHHHHHHHHHCCHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHCCCCCCCHHHHH
WLSDAHSEAPSPVSALLSAVLLNCALLVLIRYYIIICQAIGSDFPNRLLLIFGMLSVAVA
HHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHH
AFFILVQRDIKRLLAYSSVENMGLVAVALGIGGPLGIFAALLHTLNHSLAKTLLFCGSGN
HHHHHHHHHHHHHHHHHHHCCCCHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHCCCCC
VLLKYGTRDLNVVCGMLKIMPFTAVLFGGGALALAGMPPFNIFLSEFMTVTAGLARNHLL
EEEEECCCHHHHHHHHHHHHHHHHHHHCCCHHEECCCCHHHHHHHHHHHHHHHHHHHHHH
IIVLLLLLLTLVLAGLVRMAARVLMAKPPQAVNRGELGWLTTSPMVILLVMMLAMGTHIP
HHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCEECCCHHHHHHHHHHHHCCCCC
QPVIRILAGASTIVLSGTHDLPAQRSTWHDFLPSGTASVSEKHSER
HHHHHHHHCCCEEEEECCCCCCCCCCHHHHHCCCCCCHHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 9205837; 9278503 [H]