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The map label for this gene is gcvR

Identifier: 218700937

GI number: 218700937

Start: 2716630

End: 2717202

Strand: Direct

Name: gcvR

Synonym: ECIAI39_2618

Alternate gene names: 218700937

Gene position: 2716630-2717202 (Clockwise)

Preceding gene: 218700930

Following gene: 218700938

Centisome position: 52.93

GC content: 50.09

Gene sequence:

>573_bases
TTGACACTGTCATCGCAACATTATCTGGTGATCACTGCGTTGGGTGCCGATCGCCCTGGAATAGTGAACACCATCACCCG
TCATGTCAGTAGTTGCGGCTGTAATATTGAAGACAGTCGCCTGGCGATGCTGGGAGAAGAGTTCACGTTTATCATGCTGC
TTTCCGGTTCATGGAATGCCATTACTCTGATTGAATCAACGTTACCGTTGAAAGGTGCCGAACTGGATCTTTTAATCGTG
ATGAAGCGCACGACGGCGCGTCCGCGTCCGCCAATGCCAGCATCTGTCTGGGTTCAGGTCGATGTGGCAGACTCCCCGCA
TTTAATTGAACGCTTCACAGCACTTTTCGACGCGCATCATATGAACATTGCGGAGCTGGTGTCACGCACGCAACCTGCTG
AAAATGAACGGGCTGCGCAGTTGCATATTCAGATAACCGCCCACAGCCCCGCATCTGCGGACGCAGCAAATATTGAGCAA
GCGTTCAAAGCCCTATGTACAGAACTCAATGCACAAGGCAGTATTAACGTCGTCAATTATTCCCAACATGATGAACAGGA
TGGAGTTAAGTAA

Upstream 100 bases:

>100_bases
TGGCATTAAAAGCAAGCAGACAGAACCGTTCTGATTGTTGTATGCATGTTTTTTTTATGCTTTCCTTAAGAACAACTCAC
CCCTTGAAGGAATAACCAGT

Downstream 100 bases:

>100_bases
TGAATCCACTGAAAGCCGGTGATATCGCACCGAAATTTAGCTTGCCGGATCAAGACGGAGAACAAGTTAATTTGACCGAC
TTCCAGGGACAGCGTGTTCT

Product: glycine cleavage system transcriptional repressor

Products: NA

Alternate protein names: Gcv operon repressor

Number of amino acids: Translated: 190; Mature: 189

Protein sequence:

>190_residues
MTLSSQHYLVITALGADRPGIVNTITRHVSSCGCNIEDSRLAMLGEEFTFIMLLSGSWNAITLIESTLPLKGAELDLLIV
MKRTTARPRPPMPASVWVQVDVADSPHLIERFTALFDAHHMNIAELVSRTQPAENERAAQLHIQITAHSPASADAANIEQ
AFKALCTELNAQGSINVVNYSQHDEQDGVK

Sequences:

>Translated_190_residues
MTLSSQHYLVITALGADRPGIVNTITRHVSSCGCNIEDSRLAMLGEEFTFIMLLSGSWNAITLIESTLPLKGAELDLLIV
MKRTTARPRPPMPASVWVQVDVADSPHLIERFTALFDAHHMNIAELVSRTQPAENERAAQLHIQITAHSPASADAANIEQ
AFKALCTELNAQGSINVVNYSQHDEQDGVK
>Mature_189_residues
TLSSQHYLVITALGADRPGIVNTITRHVSSCGCNIEDSRLAMLGEEFTFIMLLSGSWNAITLIESTLPLKGAELDLLIVM
KRTTARPRPPMPASVWVQVDVADSPHLIERFTALFDAHHMNIAELVSRTQPAENERAAQLHIQITAHSPASADAANIEQA
FKALCTELNAQGSINVVNYSQHDEQDGVK

Specific function: Negative transcriptional regulator of the glycine cleavage system operon (GCV). Does not autoregulate its own expression. It is not yet known how gcvR acts as a repressor. It does not seem to bind DNA. It could interact with gcvA and suppress its activato

COG id: COG2716

COG function: function code E; Glycine cleavage system regulatory protein

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: NA

Homologues:

Organism=Escherichia coli, GI87082113, Length=190, Percent_Identity=100, Blast_Score=394, Evalue=1e-111,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): GCVR_ECOLI (P0A9I3)

Other databases:

- EMBL:   AF023337
- EMBL:   U00096
- EMBL:   AP009048
- EMBL:   M63654
- RefSeq:   AP_003065.1
- RefSeq:   NP_416974.4
- ProteinModelPortal:   P0A9I3
- SMR:   P0A9I3
- STRING:   P0A9I3
- EnsemblBacteria:   EBESCT00000001073
- EnsemblBacteria:   EBESCT00000015365
- GeneID:   946950
- GenomeReviews:   AP009048_GR
- GenomeReviews:   U00096_GR
- KEGG:   ecj:JW2464
- KEGG:   eco:b2479
- EchoBASE:   EB1138
- EcoGene:   EG11149
- eggNOG:   COG2716
- GeneTree:   EBGT00050000009516
- HOGENOM:   HBG700622
- OMA:   RASHENR
- ProtClustDB:   PRK11589
- BioCyc:   EcoCyc:EG11149-MONOMER
- Genevestigator:   P0A9I3
- GO:   GO:0005737
- GO:   GO:0006350
- InterPro:   IPR002912
- InterPro:   IPR016867
- PIRSF:   PIRSF028103

Pfam domain/function: PF01842 ACT

EC number: NA

Molecular weight: Translated: 20769; Mature: 20638

Theoretical pI: Translated: 5.49; Mature: 5.49

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.6 %Cys     (Translated Protein)
3.2 %Met     (Translated Protein)
4.7 %Cys+Met (Translated Protein)
1.6 %Cys     (Mature Protein)
2.6 %Met     (Mature Protein)
4.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTLSSQHYLVITALGADRPGIVNTITRHVSSCGCNIEDSRLAMLGEEFTFIMLLSGSWNA
CCCCCCCEEEEEEECCCCCCHHHHHHHHHHHCCCCCCCCHHEECCCCEEEEEEEECCCCE
ITLIESTLPLKGAELDLLIVMKRTTARPRPPMPASVWVQVDVADSPHLIERFTALFDAHH
EEEEECCCCCCCCCEEEEEEEECCCCCCCCCCCCEEEEEEEECCCCHHHHHHHHHHHHCC
MNIAELVSRTQPAENERAAQLHIQITAHSPASADAANIEQAFKALCTELNAQGSINVVNY
CCHHHHHHHCCCCCCCCEEEEEEEEEECCCCCCCHHHHHHHHHHHHHHCCCCCCEEEEEC
SQHDEQDGVK
CCCCCCCCCC
>Mature Secondary Structure 
TLSSQHYLVITALGADRPGIVNTITRHVSSCGCNIEDSRLAMLGEEFTFIMLLSGSWNA
CCCCCCEEEEEEECCCCCCHHHHHHHHHHHCCCCCCCCHHEECCCCEEEEEEEECCCCE
ITLIESTLPLKGAELDLLIVMKRTTARPRPPMPASVWVQVDVADSPHLIERFTALFDAHH
EEEEECCCCCCCCCEEEEEEEECCCCCCCCCCCCEEEEEEEECCCCHHHHHHHHHHHHCC
MNIAELVSRTQPAENERAAQLHIQITAHSPASADAANIEQAFKALCTELNAQGSINVVNY
CCHHHHHHHCCCCCCCCEEEEEEEEEECCCCCCCHHHHHHHHHHHHHHCCCCCCEEEEEC
SQHDEQDGVK
CCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 9537378; 9205837; 9278503; 2016588