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The map label for this gene is dapE

Identifier: 218700929

GI number: 218700929

Start: 2708360

End: 2709487

Strand: Direct

Name: dapE

Synonym: ECIAI39_2610

Alternate gene names: 218700929

Gene position: 2708360-2709487 (Clockwise)

Preceding gene: 218700928

Following gene: 218700930

Centisome position: 52.77

GC content: 53.9

Gene sequence:

>1128_bases
ATGTCGTGCCCGGTTATTGAGCTGACACAACAGCTTATTCGCCGCCCTTCCCTGAGTCCTGATGACGCAGGATGCCAGGC
TTTGTTGATTGAACGTTTGCAGGCGATCGGTTTTACCGTTGAACGCATGGACTTTGCCGATACGCAGAATTTTTGGGCAT
GGCGTGGGCAGGGTGAAACGTTAGCCTTTGCCGGGCATACCGACGTGGTGCCGCCTGGCGACGCCGACCGTTGGATCAAT
CCGCCGTTTGAACCCACCATTCGTGACGGCATGTTATTCGGGCGCGGTGCGGCAGATATGAAAGGCTCGCTGGCAGCGAT
GGTGGTAGCGGCAGAACGCTTTGTCGCACAACATCCCAACCATGCAGGTCGACTGGCATTTCTGATCACCTCTGATGAAG
AAGCCAGTGCCCACAACGGTACGGTAAAAGTCGTCGAGGCGTTAATGGCACGTAATGAGCGTCTCGATTACTGCCTGGTC
GGCGAACCGTCGAGTATCGAAGTGGTAGGTGATGTGGTGAAAAATGGTCGTCGCGGATCGTTAACCTGCAACCTCACCAT
TCATGGCGTGCAGGGGCATGTTGCCTACCCACATCTGGCTGACAATCCGGTACATCGCGCAGCACCTTTCCTTAATGAAT
TAGTGGCTATTGAGTGGGATCAGGGCAATGAATTCTTCCCGGCGACCAGTATGCAGATTGCCAATATTCAGGCGGGAACG
GGCAGTAACAACGTTATTCCGGGTGAACTGTTTGTGCAGTTTAACTTCCGCTTCAGCACCGAACTGACTGATGAGATGAT
CAAAGCGCAGGTACTTGCCCTGCTTGAAAAACATCAACTGCGCTATACGGTGGATTGGTGGCTTTCCGGGCAGCCATTTT
TGACCGCGCGCGGTAAACTGGTGGATGCGGTCGTTAACGCGGTTGAGCACTATAATGAAATTAAACCGCAGCTACTGACC
ACAGGCGGAACGTCCGACGGGCGCTTTATTGCCCGCATGGGGGCGCAGGTGGTGGAACTCGGGCCGGTCAATGCCACGAT
TCATAAAATTAATGAATGTGTGAACGCTGCCGACCTGCAGCTACTTGCCCGTATGTATCAACGTATCATGGAACAGCTCG
TCGCCTGA

Upstream 100 bases:

>100_bases
TGCAATTATCAAACGTCCATTGCTCTGCGCGCCAGGTAAGCCTATGCTGCTGGGTTTCAGTGAATCCAGTTATCAGCAAT
TTTTCCATGAGGTGTAGTCT

Downstream 100 bases:

>100_bases
TGAGTGGTTCTGCAAGAGGAAATAAGCATGGACTGGCTGGCTAAATATTGGTGGATTCTGGTGATTGTCTTTTTGGTAGG
CGTCCTGCTGAACGTGATTA

Product: succinyl-diaminopimelate desuccinylase

Products: NA

Alternate protein names: SDAP desuccinylase; N-succinyl-LL-2,6-diaminoheptanedioate amidohydrolase [H]

Number of amino acids: Translated: 375; Mature: 374

Protein sequence:

>375_residues
MSCPVIELTQQLIRRPSLSPDDAGCQALLIERLQAIGFTVERMDFADTQNFWAWRGQGETLAFAGHTDVVPPGDADRWIN
PPFEPTIRDGMLFGRGAADMKGSLAAMVVAAERFVAQHPNHAGRLAFLITSDEEASAHNGTVKVVEALMARNERLDYCLV
GEPSSIEVVGDVVKNGRRGSLTCNLTIHGVQGHVAYPHLADNPVHRAAPFLNELVAIEWDQGNEFFPATSMQIANIQAGT
GSNNVIPGELFVQFNFRFSTELTDEMIKAQVLALLEKHQLRYTVDWWLSGQPFLTARGKLVDAVVNAVEHYNEIKPQLLT
TGGTSDGRFIARMGAQVVELGPVNATIHKINECVNAADLQLLARMYQRIMEQLVA

Sequences:

>Translated_375_residues
MSCPVIELTQQLIRRPSLSPDDAGCQALLIERLQAIGFTVERMDFADTQNFWAWRGQGETLAFAGHTDVVPPGDADRWIN
PPFEPTIRDGMLFGRGAADMKGSLAAMVVAAERFVAQHPNHAGRLAFLITSDEEASAHNGTVKVVEALMARNERLDYCLV
GEPSSIEVVGDVVKNGRRGSLTCNLTIHGVQGHVAYPHLADNPVHRAAPFLNELVAIEWDQGNEFFPATSMQIANIQAGT
GSNNVIPGELFVQFNFRFSTELTDEMIKAQVLALLEKHQLRYTVDWWLSGQPFLTARGKLVDAVVNAVEHYNEIKPQLLT
TGGTSDGRFIARMGAQVVELGPVNATIHKINECVNAADLQLLARMYQRIMEQLVA
>Mature_374_residues
SCPVIELTQQLIRRPSLSPDDAGCQALLIERLQAIGFTVERMDFADTQNFWAWRGQGETLAFAGHTDVVPPGDADRWINP
PFEPTIRDGMLFGRGAADMKGSLAAMVVAAERFVAQHPNHAGRLAFLITSDEEASAHNGTVKVVEALMARNERLDYCLVG
EPSSIEVVGDVVKNGRRGSLTCNLTIHGVQGHVAYPHLADNPVHRAAPFLNELVAIEWDQGNEFFPATSMQIANIQAGTG
SNNVIPGELFVQFNFRFSTELTDEMIKAQVLALLEKHQLRYTVDWWLSGQPFLTARGKLVDAVVNAVEHYNEIKPQLLTT
GGTSDGRFIARMGAQVVELGPVNATIHKINECVNAADLQLLARMYQRIMEQLVA

Specific function: Catalyzes the hydrolysis of N-succinyl-L,L- diaminopimelic acid (SDAP), forming succinate and LL-2,6- diaminoheptanedioate (DAP), an intermediate involved in the bacterial biosynthesis of lysine and meso-diaminopimelic acid, an essential component of bact

COG id: COG0624

COG function: function code E; Acetylornithine deacetylase/Succinyl-diaminopimelate desuccinylase and related deacylases

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the peptidase M20A family. DapE subfamily [H]

Homologues:

Organism=Escherichia coli, GI1788816, Length=375, Percent_Identity=99.7333333333333, Blast_Score=776, Evalue=0.0,
Organism=Escherichia coli, GI1790395, Length=259, Percent_Identity=28.957528957529, Blast_Score=79, Evalue=5e-16,
Organism=Escherichia coli, GI1789236, Length=385, Percent_Identity=23.1168831168831, Blast_Score=72, Evalue=5e-14,
Organism=Caenorhabditis elegans, GI17551016, Length=281, Percent_Identity=26.3345195729537, Blast_Score=70, Evalue=2e-12,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001261
- InterPro:   IPR005941
- InterPro:   IPR002933
- InterPro:   IPR011650 [H]

Pfam domain/function: PF07687 M20_dimer; PF01546 Peptidase_M20 [H]

EC number: =3.5.1.18 [H]

Molecular weight: Translated: 41240; Mature: 41108

Theoretical pI: Translated: 5.23; Mature: 5.23

Prosite motif: PS00758 ARGE_DAPE_CPG2_1 ; PS00759 ARGE_DAPE_CPG2_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.3 %Cys     (Translated Protein)
2.9 %Met     (Translated Protein)
4.3 %Cys+Met (Translated Protein)
1.3 %Cys     (Mature Protein)
2.7 %Met     (Mature Protein)
4.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSCPVIELTQQLIRRPSLSPDDAGCQALLIERLQAIGFTVERMDFADTQNFWAWRGQGET
CCCCHHHHHHHHHHCCCCCCCCCCHHHHHHHHHHHHCCCHHHCCCCCCCCCEEECCCCCE
LAFAGHTDVVPPGDADRWINPPFEPTIRDGMLFGRGAADMKGSLAAMVVAAERFVAQHPN
EEEECCCCCCCCCCCCCCCCCCCCCCHHCCEEECCCCCCCCCHHHHHHHHHHHHHHHCCC
HAGRLAFLITSDEEASAHNGTVKVVEALMARNERLDYCLVGEPSSIEVVGDVVKNGRRGS
CCCEEEEEEECCCCCCCCCCHHHHHHHHHHCCCCCCEEEECCCCCHHHHHHHHHCCCCCE
LTCNLTIHGVQGHVAYPHLADNPVHRAAPFLNELVAIEWDQGNEFFPATSMQIANIQAGT
EEEEEEEEECCCCEECCCCCCCCHHHHHHHHHCEEEEEECCCCEECCCCCEEEEEEECCC
GSNNVIPGELFVQFNFRFSTELTDEMIKAQVLALLEKHQLRYTVDWWLSGQPFLTARGKL
CCCCCCCCEEEEEEEEEECCHHHHHHHHHHHHHHHHHCCEEEEEEEEECCCCEEEECHHH
VDAVVNAVEHYNEIKPQLLTTGGTSDGRFIARMGAQVVELGPVNATIHKINECVNAADLQ
HHHHHHHHHHHHHCCCEEEECCCCCCCCHHHHHCCEEEEECCCCHHHHHHHHHHCHHHHH
LLARMYQRIMEQLVA
HHHHHHHHHHHHHCC
>Mature Secondary Structure 
SCPVIELTQQLIRRPSLSPDDAGCQALLIERLQAIGFTVERMDFADTQNFWAWRGQGET
CCCHHHHHHHHHHCCCCCCCCCCHHHHHHHHHHHHCCCHHHCCCCCCCCCEEECCCCCE
LAFAGHTDVVPPGDADRWINPPFEPTIRDGMLFGRGAADMKGSLAAMVVAAERFVAQHPN
EEEECCCCCCCCCCCCCCCCCCCCCCHHCCEEECCCCCCCCCHHHHHHHHHHHHHHHCCC
HAGRLAFLITSDEEASAHNGTVKVVEALMARNERLDYCLVGEPSSIEVVGDVVKNGRRGS
CCCEEEEEEECCCCCCCCCCHHHHHHHHHHCCCCCCEEEECCCCCHHHHHHHHHCCCCCE
LTCNLTIHGVQGHVAYPHLADNPVHRAAPFLNELVAIEWDQGNEFFPATSMQIANIQAGT
EEEEEEEEECCCCEECCCCCCCCHHHHHHHHHCEEEEEECCCCEECCCCCEEEEEEECCC
GSNNVIPGELFVQFNFRFSTELTDEMIKAQVLALLEKHQLRYTVDWWLSGQPFLTARGKL
CCCCCCCCEEEEEEEEEECCHHHHHHHHHHHHHHHHHCCEEEEEEEEECCCCEEEECHHH
VDAVVNAVEHYNEIKPQLLTTGGTSDGRFIARMGAQVVELGPVNATIHKINECVNAADLQ
HHHHHHHHHHHHHCCCEEEECCCCCCCCHHHHHCCEEEEECCCCHHHHHHHHHHCHHHHH
LLARMYQRIMEQLVA
HHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA