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The map label for this gene is murP

Identifier: 218700895

GI number: 218700895

Start: 2670523

End: 2671947

Strand: Direct

Name: murP

Synonym: ECIAI39_2574

Alternate gene names: 218700895

Gene position: 2670523-2671947 (Clockwise)

Preceding gene: 218700894

Following gene: 218700896

Centisome position: 52.04

GC content: 55.23

Gene sequence:

>1425_bases
ATGGCCAAAGAGATCAGCAGTGAACTTCTGAACACCATTCTTACCCGGGTCGGTGGACCGGGAAATATCGCCAGTTGTGG
TAACTGTATGACGCGCCTGCGTCTGGGTGTACATGACAGTTCACTGGTTGATCCCAATATCAAAACGCTGGAAGGCGTGA
AGGGCGTTATTTTGACCAGCGATCAGGTACAGGTAGTTTTTGGACCAGGTAAAGCGCATCGTGCCGCAAAAGCGATGAGC
GAACTGCTGGGAGAAGCCCCGGTACAAGATGCCGCAGAAATCGCCGCTCAGAATAAACGTCAGTTAAAAGCCAAACAAAC
CTCCGGCGTGCAACAATTTCTCGCCAAATTCGCCACCATCTTCACGCCGCTGATCCCCGGTTTTATTGCCGCTGGTCTGT
TGCTGGGAATAGCGACGTTAATTGCCACGGTGATGCACGTTCCGGCAGATGCTCAGGGCACGCTACCCGATGCGCTGAAT
TTTATGAAGGTGTTCAGCAAAGGCCTGTTCACCTTCCTGGTAATTCTGGTGGGCTATAACGCGGCCCAGGCATTTGGTGG
CACGGGCGTAAATGGCGCAATTATCGCTGCGCTCTTTTTGCTCGGTTATAACCCAACTGCAACCACGGGTTACTACGCCG
GATTTCACGATTTCTTTGGTCTGCCTATCGATCCGCGCGGCAATATTATCGGCGTGTTGATTACCGCATGGGCCTGCGCC
CGCATCGAATGCATGGTGCACCGCTTTATGCCGGACGACCTCGACATGCTGTTGACCTCATTAATCACCCTGCTGATCAC
CGCTACGCTGGCCTACCTGATCATTATGCCGCTGGGCGGCTGGCTGTTTGAAGGTATGTCATGGCTGTTTATGCACCTGA
ACAGTAATCCGCTCGGTTGTGCGGTTTTAGCCGGGCTGTTCCTGATCGCCGTGGTATTTGGCGTGCATCAGGGCTTTATT
CCTGTTTATCTGGCGTTAATGGACAGCCAGGGATTCAACAGCTTATTTCCCATCCTTTCAATGGCAGGCGCAGGCCAGGT
GGGCGCGGCGCTGGCGCTCTACTGGCGGGCGCAACCGCACAGTGCATTACGCAGTCAGGTCCGCGGGGCGATTATTCCTG
GTCTGCTTGGCGTTGGCGAACCGCTGATTTACGGTGTCACCCTGCCCCGCATGAAACCGTTTGTTACCGCCTGTTTAGGC
GGCGCGGCGGGCGGTTTGTTTATCGGACTGATAGCCTGGTGGGGTCTGCCGATGGGCTTAAACAGCGCATTCGGGCCGTC
TGGTCTGGTGGCGCTGCCGCTGATGACTTCCGCGCAAGGCATCCTTCCGGCAATGGCGGTTTATGCAGGCGGGATTCTGG
TGGCATGGGTTTGCGGGTTTATTTTCACCACGCTCTTTGGCTGCCGTAACGTCAATCTGGACTGA

Upstream 100 bases:

>100_bases
CATTGTGATGGTGCTGAAAAATCTCGATGCCGCAGAAGCAAAAAAACGCCTGGATCAACACGGCGGCTTTATTCGTCAGG
TTTTAGACAAGGAATAACCC

Downstream 100 bases:

>100_bases
TAATATGAAACGGACAATGCTCTATCTTTCTCTGCTGGCTGTCAGCTGTAGCGTCAGCGCCGCAAAATACCCTGTTCTGA
CAGAAAGTTCGCCAGAGAAA

Product: PTS system N-acetylmuramic acid transporter subunits EIIBC

Products: NA

Alternate protein names: EIIBC-MurNAc; N-acetylmuramic acid-specific phosphotransferase enzyme IIB component; PTS system N-acetylmuramic acid-specific EIIB component; N-acetylmuramic acid permease IIC component; PTS system N-acetylmuramic acid-specific EIIC component [H]

Number of amino acids: Translated: 474; Mature: 473

Protein sequence:

>474_residues
MAKEISSELLNTILTRVGGPGNIASCGNCMTRLRLGVHDSSLVDPNIKTLEGVKGVILTSDQVQVVFGPGKAHRAAKAMS
ELLGEAPVQDAAEIAAQNKRQLKAKQTSGVQQFLAKFATIFTPLIPGFIAAGLLLGIATLIATVMHVPADAQGTLPDALN
FMKVFSKGLFTFLVILVGYNAAQAFGGTGVNGAIIAALFLLGYNPTATTGYYAGFHDFFGLPIDPRGNIIGVLITAWACA
RIECMVHRFMPDDLDMLLTSLITLLITATLAYLIIMPLGGWLFEGMSWLFMHLNSNPLGCAVLAGLFLIAVVFGVHQGFI
PVYLALMDSQGFNSLFPILSMAGAGQVGAALALYWRAQPHSALRSQVRGAIIPGLLGVGEPLIYGVTLPRMKPFVTACLG
GAAGGLFIGLIAWWGLPMGLNSAFGPSGLVALPLMTSAQGILPAMAVYAGGILVAWVCGFIFTTLFGCRNVNLD

Sequences:

>Translated_474_residues
MAKEISSELLNTILTRVGGPGNIASCGNCMTRLRLGVHDSSLVDPNIKTLEGVKGVILTSDQVQVVFGPGKAHRAAKAMS
ELLGEAPVQDAAEIAAQNKRQLKAKQTSGVQQFLAKFATIFTPLIPGFIAAGLLLGIATLIATVMHVPADAQGTLPDALN
FMKVFSKGLFTFLVILVGYNAAQAFGGTGVNGAIIAALFLLGYNPTATTGYYAGFHDFFGLPIDPRGNIIGVLITAWACA
RIECMVHRFMPDDLDMLLTSLITLLITATLAYLIIMPLGGWLFEGMSWLFMHLNSNPLGCAVLAGLFLIAVVFGVHQGFI
PVYLALMDSQGFNSLFPILSMAGAGQVGAALALYWRAQPHSALRSQVRGAIIPGLLGVGEPLIYGVTLPRMKPFVTACLG
GAAGGLFIGLIAWWGLPMGLNSAFGPSGLVALPLMTSAQGILPAMAVYAGGILVAWVCGFIFTTLFGCRNVNLD
>Mature_473_residues
AKEISSELLNTILTRVGGPGNIASCGNCMTRLRLGVHDSSLVDPNIKTLEGVKGVILTSDQVQVVFGPGKAHRAAKAMSE
LLGEAPVQDAAEIAAQNKRQLKAKQTSGVQQFLAKFATIFTPLIPGFIAAGLLLGIATLIATVMHVPADAQGTLPDALNF
MKVFSKGLFTFLVILVGYNAAQAFGGTGVNGAIIAALFLLGYNPTATTGYYAGFHDFFGLPIDPRGNIIGVLITAWACAR
IECMVHRFMPDDLDMLLTSLITLLITATLAYLIIMPLGGWLFEGMSWLFMHLNSNPLGCAVLAGLFLIAVVFGVHQGFIP
VYLALMDSQGFNSLFPILSMAGAGQVGAALALYWRAQPHSALRSQVRGAIIPGLLGVGEPLIYGVTLPRMKPFVTACLGG
AAGGLFIGLIAWWGLPMGLNSAFGPSGLVALPLMTSAQGILPAMAVYAGGILVAWVCGFIFTTLFGCRNVNLD

Specific function: The phosphoenolpyruvate-dependent sugar phosphotransferase system (sugar PTS), a major carbohydrate active -transport system, catalyzes the phosphorylation of incoming sugar substrates concomitantly with their translocation across the cell membrane. This

COG id: COG1263

COG function: function code G; Phosphotransferase system IIC components, glucose/maltose/N-acetylglucosamine-specific

Gene ontology:

Cell location: Cell inner membrane; Multi-pass membrane protein [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 PTS EIIC type-1 domain [H]

Homologues:

Organism=Escherichia coli, GI1788769, Length=474, Percent_Identity=98.9451476793249, Blast_Score=934, Evalue=0.0,
Organism=Escherichia coli, GI1790159, Length=435, Percent_Identity=29.6551724137931, Blast_Score=160, Evalue=2e-40,
Organism=Escherichia coli, GI2367362, Length=428, Percent_Identity=26.6355140186916, Blast_Score=142, Evalue=4e-35,
Organism=Escherichia coli, GI48994906, Length=487, Percent_Identity=25.4620123203285, Blast_Score=110, Evalue=2e-25,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR018113
- InterPro:   IPR001996
- InterPro:   IPR003352
- InterPro:   IPR013013 [H]

Pfam domain/function: PF00367 PTS_EIIB; PF02378 PTS_EIIC [H]

EC number: =2.7.1.69 [H]

Molecular weight: Translated: 49856; Mature: 49725

Theoretical pI: Translated: 8.21; Mature: 8.21

Prosite motif: PS00024 HEMOPEXIN ; PS01035 PTS_EIIB_TYPE_1_CYS ; PS51098 PTS_EIIB_TYPE_1 ; PS51103 PTS_EIIC_TYPE_1

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.7 %Cys     (Translated Protein)
3.6 %Met     (Translated Protein)
5.3 %Cys+Met (Translated Protein)
1.7 %Cys     (Mature Protein)
3.4 %Met     (Mature Protein)
5.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MAKEISSELLNTILTRVGGPGNIASCGNCMTRLRLGVHDSSLVDPNIKTLEGVKGVILTS
CCHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHCCCCCCCCCCCCCHHHHCCCCEEEEC
DQVQVVFGPGKAHRAAKAMSELLGEAPVQDAAEIAAQNKRQLKAKQTSGVQQFLAKFATI
CCEEEEECCCHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
FTPLIPGFIAAGLLLGIATLIATVMHVPADAQGTLPDALNFMKVFSKGLFTFLVILVGYN
HHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHCCC
AAQAFGGTGVNGAIIAALFLLGYNPTATTGYYAGFHDFFGLPIDPRGNIIGVLITAWACA
HHHHCCCCCCHHHHHHHHHHHCCCCCCCCCCCCCHHHHCCCCCCCCCCCHHHHHHHHHHH
RIECMVHRFMPDDLDMLLTSLITLLITATLAYLIIMPLGGWLFEGMSWLFMHLNSNPLGC
HHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHCCCCCCHHH
AVLAGLFLIAVVFGVHQGFIPVYLALMDSQGFNSLFPILSMAGAGQVGAALALYWRAQPH
HHHHHHHHHHHHHHHHCCHHHHHHHHHCCCCCHHHHHHHHHCCCCHHHHHHHHHHCCCCH
SALRSQVRGAIIPGLLGVGEPLIYGVTLPRMKPFVTACLGGAAGGLFIGLIAWWGLPMGL
HHHHHHHHHHHHHHHHHCCCCCEEECCCCCHHHHHHHHHHCCHHHHHHHHHHHHCCCCCC
NSAFGPSGLVALPLMTSAQGILPAMAVYAGGILVAWVCGFIFTTLFGCRNVNLD
CCCCCCCCCEEHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCC
>Mature Secondary Structure 
AKEISSELLNTILTRVGGPGNIASCGNCMTRLRLGVHDSSLVDPNIKTLEGVKGVILTS
CHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHCCCCCCCCCCCCCHHHHCCCCEEEEC
DQVQVVFGPGKAHRAAKAMSELLGEAPVQDAAEIAAQNKRQLKAKQTSGVQQFLAKFATI
CCEEEEECCCHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
FTPLIPGFIAAGLLLGIATLIATVMHVPADAQGTLPDALNFMKVFSKGLFTFLVILVGYN
HHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHCCC
AAQAFGGTGVNGAIIAALFLLGYNPTATTGYYAGFHDFFGLPIDPRGNIIGVLITAWACA
HHHHCCCCCCHHHHHHHHHHHCCCCCCCCCCCCCHHHHCCCCCCCCCCCHHHHHHHHHHH
RIECMVHRFMPDDLDMLLTSLITLLITATLAYLIIMPLGGWLFEGMSWLFMHLNSNPLGC
HHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHCCCCCCHHH
AVLAGLFLIAVVFGVHQGFIPVYLALMDSQGFNSLFPILSMAGAGQVGAALALYWRAQPH
HHHHHHHHHHHHHHHHCCHHHHHHHHHCCCCCHHHHHHHHHCCCCHHHHHHHHHHCCCCH
SALRSQVRGAIIPGLLGVGEPLIYGVTLPRMKPFVTACLGGAAGGLFIGLIAWWGLPMGL
HHHHHHHHHHHHHHHHHCCCCCEEECCCCCHHHHHHHHHHCCHHHHHHHHHHHHCCCCCC
NSAFGPSGLVALPLMTSAQGILPAMAVYAGGILVAWVCGFIFTTLFGCRNVNLD
CCCCCCCCCEEHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 11206551; 11258796 [H]