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The map label for this gene is evgA

Identifier: 218700837

GI number: 218700837

Start: 2605557

End: 2606171

Strand: Direct

Name: evgA

Synonym: ECIAI39_2512

Alternate gene names: 218700837

Gene position: 2605557-2606171 (Clockwise)

Preceding gene: 218700834

Following gene: 218700838

Centisome position: 50.77

GC content: 39.51

Gene sequence:

>615_bases
ATGAACGCAATAATTATTGATGACCATCCTCTTGCTATCGCAGCAATTCGTAATTTATTGATCAAAAACGATATTGAAAT
CTTAGCAGAGTTGACTGAAGGCGGAAGTGCCGTTCAGCGGGTGGAAACACTTAAACCTGATATCGTCATCATTGATGTCG
ATATCCCCGGAGTTAACGGTATCCAGGTGTTAGAAACGCTGAGAAAGCGCCAATATAGCGGAATTATTATTATTGTCTCC
GCTAAAAATGACCATTTTTACGGGAAACATTGTGCTGATGCTGGCGCTAATGGTTTCGTGAGTAAAAAAGAAGGCATGAA
CAATATCATTGCGGCTATTGAAGCTGCAAAAAATGGCTACTGCTATTTCCCCTTCTCTCTCAACCGGTTTGTTGGAAGTT
TAACGTCCGACCAGCAAAAACTCGACTCCTTATCGAAACAAGAAATTAGTGTCATGCGGTATATTCTTGATGGCAAGGAT
AATAATGACATTGCTGATAAAATGTTCATCAGCAACAAAACTGTCAGCACTTATAAAAGTCGCTTGATGGAAAAATTAGA
ATGCAAATCACTGATGGATCTTTACACATTCGCACAACGTAACAAAATCGGCTAA

Upstream 100 bases:

>100_bases
AGCTCAAATTTTCTTATTTTTATTGAGAAAATGAGATGATGCCTTATGTCTGTATTACTACAGGGAGAAGGGAAATGCTT
CATTGCAAAGGGAATAATCT

Downstream 100 bases:

>100_bases
CCACATGAAGTTTTTACCCTATATTTTTCTTCTCTGTTGTGGTCTTTGGTCGACCATAAGTTTCGCAGACAGAGATTACA
TCGAATATCGTGGCATCAGT

Product: DNA-binding transcriptional activator EvgA

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 204; Mature: 204

Protein sequence:

>204_residues
MNAIIIDDHPLAIAAIRNLLIKNDIEILAELTEGGSAVQRVETLKPDIVIIDVDIPGVNGIQVLETLRKRQYSGIIIIVS
AKNDHFYGKHCADAGANGFVSKKEGMNNIIAAIEAAKNGYCYFPFSLNRFVGSLTSDQQKLDSLSKQEISVMRYILDGKD
NNDIADKMFISNKTVSTYKSRLMEKLECKSLMDLYTFAQRNKIG

Sequences:

>Translated_204_residues
MNAIIIDDHPLAIAAIRNLLIKNDIEILAELTEGGSAVQRVETLKPDIVIIDVDIPGVNGIQVLETLRKRQYSGIIIIVS
AKNDHFYGKHCADAGANGFVSKKEGMNNIIAAIEAAKNGYCYFPFSLNRFVGSLTSDQQKLDSLSKQEISVMRYILDGKD
NNDIADKMFISNKTVSTYKSRLMEKLECKSLMDLYTFAQRNKIG
>Mature_204_residues
MNAIIIDDHPLAIAAIRNLLIKNDIEILAELTEGGSAVQRVETLKPDIVIIDVDIPGVNGIQVLETLRKRQYSGIIIIVS
AKNDHFYGKHCADAGANGFVSKKEGMNNIIAAIEAAKNGYCYFPFSLNRFVGSLTSDQQKLDSLSKQEISVMRYILDGKD
NNDIADKMFISNKTVSTYKSRLMEKLECKSLMDLYTFAQRNKIG

Specific function: Member of the two-component regulatory system evgS/evgA. Regulates the expression of emrKY operon and yfdX. Seems also to control expression of at least one other multidrug efflux operon [H]

COG id: COG2197

COG function: function code TK; Response regulator containing a CheY-like receiver domain and an HTH DNA-binding domain

Gene ontology:

Cell location: Cytoplasmic [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 response regulatory domain [H]

Homologues:

Organism=Escherichia coli, GI1788712, Length=204, Percent_Identity=99.5098039215686, Blast_Score=415, Evalue=1e-117,
Organism=Escherichia coli, GI1786747, Length=201, Percent_Identity=32.8358208955224, Blast_Score=126, Evalue=8e-31,
Organism=Escherichia coli, GI1788222, Length=207, Percent_Identity=27.0531400966184, Blast_Score=89, Evalue=2e-19,
Organism=Escherichia coli, GI1788521, Length=194, Percent_Identity=28.8659793814433, Blast_Score=86, Evalue=2e-18,
Organism=Escherichia coli, GI1790102, Length=183, Percent_Identity=28.9617486338798, Blast_Score=80, Evalue=1e-16,
Organism=Escherichia coli, GI1787473, Length=181, Percent_Identity=24.8618784530387, Blast_Score=68, Evalue=4e-13,
Organism=Escherichia coli, GI1788546, Length=190, Percent_Identity=26.3157894736842, Blast_Score=68, Evalue=6e-13,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR011006
- InterPro:   IPR016032
- InterPro:   IPR001789
- InterPro:   IPR000792
- InterPro:   IPR011991 [H]

Pfam domain/function: PF00196 GerE; PF00072 Response_reg [H]

EC number: NA

Molecular weight: Translated: 22676; Mature: 22676

Theoretical pI: Translated: 7.43; Mature: 7.43

Prosite motif: PS50110 RESPONSE_REGULATORY ; PS00622 HTH_LUXR_1 ; PS50043 HTH_LUXR_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.5 %Cys     (Translated Protein)
2.9 %Met     (Translated Protein)
4.4 %Cys+Met (Translated Protein)
1.5 %Cys     (Mature Protein)
2.9 %Met     (Mature Protein)
4.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNAIIIDDHPLAIAAIRNLLIKNDIEILAELTEGGSAVQRVETLKPDIVIIDVDIPGVNG
CCEEEECCCCHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHCCCCEEEEEECCCCCCH
IQVLETLRKRQYSGIIIIVSAKNDHFYGKHCADAGANGFVSKKEGMNNIIAAIEAAKNGY
HHHHHHHHHHCCCCEEEEEECCCCCCCCCHHHCCCCCCCCCCHHHHHHHHHHHHHCCCCE
CYFPFSLNRFVGSLTSDQQKLDSLSKQEISVMRYILDGKDNNDIADKMFISNKTVSTYKS
EEECCCHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHCCCHHHHHHH
RLMEKLECKSLMDLYTFAQRNKIG
HHHHHHHHHHHHHHHHHHHHCCCC
>Mature Secondary Structure
MNAIIIDDHPLAIAAIRNLLIKNDIEILAELTEGGSAVQRVETLKPDIVIIDVDIPGVNG
CCEEEECCCCHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHCCCCEEEEEECCCCCCH
IQVLETLRKRQYSGIIIIVSAKNDHFYGKHCADAGANGFVSKKEGMNNIIAAIEAAKNGY
HHHHHHHHHHCCCCEEEEEECCCCCCCCCHHHCCCCCCCCCCHHHHHHHHHHHHHCCCCE
CYFPFSLNRFVGSLTSDQQKLDSLSKQEISVMRYILDGKDNNDIADKMFISNKTVSTYKS
EEECCCHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHCCCHHHHHHH
RLMEKLECKSLMDLYTFAQRNKIG
HHHHHHHHHHHHHHHHHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: DNA [C]

Specific reaction: Protein + DNA = Protein-DNA [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 11206551; 11258796 [H]