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The map label for this gene is intS

Identifier: 218700826

GI number: 218700826

Start: 2588734

End: 2589891

Strand: Direct

Name: intS

Synonym: ECIAI39_2501

Alternate gene names: 218700826

Gene position: 2588734-2589891 (Clockwise)

Preceding gene: 218700825

Following gene: 218700827

Centisome position: 50.44

GC content: 47.84

Gene sequence:

>1158_bases
ATGCTCACCGTTAAGCAGATTGAAGCAGCAAAGCCGAAAGAAAAACCATACCGCCTACTCGATGGTAATGGCCTGTACCT
TTATGTCCCTGTGTCAGGGAAAAAGGTATGGCAGCTTCGCTACAAGATTGACGGTAAGGAGAAAATCCTGACCGTAGGAA
AATATCCGCTAATGACTTTGCAGGAGGCAAGAGATAAAGCATGGACCGCGAGGAAAGACATCTCGGTTGGCATCGATCCG
GTAAAAGCGAAAAAGGCTTCGTCTAACAACAATTCCTTTAGCGCCATTTACAAGGAATGGTACGAGCACAAGAAGCAAGT
CTGGTCAGTAGGCTATGCAACTGAACTTGCCAAAATGTTTGATGACGACATTTTGCCCATCATCGGCGGTCTTGAGATTC
AGGATATTGAGCCGATGCAACTGCTGGAAGTAATCCGCAGATTTGAAGATCGCGGTGCAATGGAACGAGCCAATAAAGCC
CGCAGAAGATGCGGCGAGGTTTTTCGTTACGCTATTGTCACCGGAAGGGCTAAATATAACCCGGCACCTGACCTTGCAGA
CGCCATGAAAGGATACCGCAAGAAGAACTTCCCGTTTCTTCCAGCAGACCAGATCCCTGCATTCAACAAAGCACTGGCAA
CATTTTCAGGAAGTATCGTATCGCTCATTGCGACCAAAGTCTTACGCTACACAGCCCTAAGAACGAAAGAGCTTCGTTCC
ATGCAATGGAAGAGCGTCGATTTTGAAAACAGGATTATCACCATCGACGCCAGTGTGATGAAGGGACGCAAAATTCATGT
GGTTCCTATGTCAGACCAGGTGGTTGAACTTCTTACTACGCTAAGCTCAATCACCAAACCAGTCTCAGAGTTTGTTTTTG
CCGGGCGCAACGATAAGAAGAAACCAATCTGCGATAACGCGGTATTGCTTGTGATCAAACAAATCGGCTATGAGGGGCTG
GAAAGCGGTCACGGATTCAGGCATGAATTCAGCACGATTATGAACGAGCACGAATGGCCTGCCGACGCCATTGAAGTGCA
ACTGGCACATGCCAACGGCGGATCTGTACGTGGAATTTACAACCATGCTCAGTATCTCGATAAGCGCAGAGAAATGATGC
AGTGGTGGGCGGATTGGCTTGATGAGAAGGTAGAGTGA

Upstream 100 bases:

>100_bases
AGATTCACAGTTCGTAATGGTTCGCGTCAGATCGTTGACAGCCGCACTCCATGACGGGTAAAAAGTGGATAAAATAATTT
TACCCACCGGATTTTTACCC

Downstream 100 bases:

>100_bases
TCCGCCTTAACAACTATCGAAGAGCACAAAGCCTTGTGTGTCTCATAGGCTATGAAACAATCATTTTACATGGTTACTTC
ACTACTTCCGCACAATATGA

Product: putative prophage CPS-53 integrase; CPS-53 (KpLE1) prophage

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 385; Mature: 385

Protein sequence:

>385_residues
MLTVKQIEAAKPKEKPYRLLDGNGLYLYVPVSGKKVWQLRYKIDGKEKILTVGKYPLMTLQEARDKAWTARKDISVGIDP
VKAKKASSNNNSFSAIYKEWYEHKKQVWSVGYATELAKMFDDDILPIIGGLEIQDIEPMQLLEVIRRFEDRGAMERANKA
RRRCGEVFRYAIVTGRAKYNPAPDLADAMKGYRKKNFPFLPADQIPAFNKALATFSGSIVSLIATKVLRYTALRTKELRS
MQWKSVDFENRIITIDASVMKGRKIHVVPMSDQVVELLTTLSSITKPVSEFVFAGRNDKKKPICDNAVLLVIKQIGYEGL
ESGHGFRHEFSTIMNEHEWPADAIEVQLAHANGGSVRGIYNHAQYLDKRREMMQWWADWLDEKVE

Sequences:

>Translated_385_residues
MLTVKQIEAAKPKEKPYRLLDGNGLYLYVPVSGKKVWQLRYKIDGKEKILTVGKYPLMTLQEARDKAWTARKDISVGIDP
VKAKKASSNNNSFSAIYKEWYEHKKQVWSVGYATELAKMFDDDILPIIGGLEIQDIEPMQLLEVIRRFEDRGAMERANKA
RRRCGEVFRYAIVTGRAKYNPAPDLADAMKGYRKKNFPFLPADQIPAFNKALATFSGSIVSLIATKVLRYTALRTKELRS
MQWKSVDFENRIITIDASVMKGRKIHVVPMSDQVVELLTTLSSITKPVSEFVFAGRNDKKKPICDNAVLLVIKQIGYEGL
ESGHGFRHEFSTIMNEHEWPADAIEVQLAHANGGSVRGIYNHAQYLDKRREMMQWWADWLDEKVE
>Mature_385_residues
MLTVKQIEAAKPKEKPYRLLDGNGLYLYVPVSGKKVWQLRYKIDGKEKILTVGKYPLMTLQEARDKAWTARKDISVGIDP
VKAKKASSNNNSFSAIYKEWYEHKKQVWSVGYATELAKMFDDDILPIIGGLEIQDIEPMQLLEVIRRFEDRGAMERANKA
RRRCGEVFRYAIVTGRAKYNPAPDLADAMKGYRKKNFPFLPADQIPAFNKALATFSGSIVSLIATKVLRYTALRTKELRS
MQWKSVDFENRIITIDASVMKGRKIHVVPMSDQVVELLTTLSSITKPVSEFVFAGRNDKKKPICDNAVLLVIKQIGYEGL
ESGHGFRHEFSTIMNEHEWPADAIEVQLAHANGGSVRGIYNHAQYLDKRREMMQWWADWLDEKVE

Specific function: Integrase is necessary for integration of the phage into the host genome by site-specific recombination. In conjunction with excisionase, integrase is also necessary for excision of the prophage from the host genome [H]

COG id: COG0582

COG function: function code L; Integrase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the 'phage' integrase family [H]

Homologues:

Organism=Escherichia coli, GI1788690, Length=385, Percent_Identity=99.2207792207792, Blast_Score=791, Evalue=0.0,
Organism=Escherichia coli, GI145693166, Length=389, Percent_Identity=40.6169665809769, Blast_Score=275, Evalue=3e-75,
Organism=Escherichia coli, GI1788974, Length=388, Percent_Identity=32.9896907216495, Blast_Score=211, Evalue=9e-56,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR011010
- InterPro:   IPR013762
- InterPro:   IPR002104
- InterPro:   IPR023109 [H]

Pfam domain/function: PF00589 Phage_integrase [H]

EC number: NA

Molecular weight: Translated: 44035; Mature: 44035

Theoretical pI: Translated: 9.91; Mature: 9.91

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.5 %Cys     (Translated Protein)
3.1 %Met     (Translated Protein)
3.6 %Cys+Met (Translated Protein)
0.5 %Cys     (Mature Protein)
3.1 %Met     (Mature Protein)
3.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MLTVKQIEAAKPKEKPYRLLDGNGLYLYVPVSGKKVWQLRYKIDGKEKILTVGKYPLMTL
CCCHHHHHCCCCCCCCCEEECCCCEEEEEEECCCEEEEEEEEECCCCEEEEECCCCHHHH
QEARDKAWTARKDISVGIDPVKAKKASSNNNSFSAIYKEWYEHKKQVWSVGYATELAKMF
HHHHHHHHHHHCCCCCCCCCCHHCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
DDDILPIIGGLEIQDIEPMQLLEVIRRFEDRGAMERANKARRRCGEVFRYAIVTGRAKYN
CCCCHHHHCCEEECCCCHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHCCCCCCC
PAPDLADAMKGYRKKNFPFLPADQIPAFNKALATFSGSIVSLIATKVLRYTALRTKELRS
CCCCHHHHHHHHHCCCCCCCCCCCCCHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHH
MQWKSVDFENRIITIDASVMKGRKIHVVPMSDQVVELLTTLSSITKPVSEFVFAGRNDKK
HHCCCCCCCCEEEEEEHHHHCCCEEEEEECCHHHHHHHHHHHHHHHHHHHHHHCCCCCCC
KPICDNAVLLVIKQIGYEGLESGHGFRHEFSTIMNEHEWPADAIEVQLAHANGGSVRGIY
CCCCCHHHHHHHHHHCHHHHCCCCCHHHHHHHHHCCCCCCCCCEEEEEEECCCCCCCHHH
NHAQYLDKRREMMQWWADWLDEKVE
HHHHHHHHHHHHHHHHHHHHHHCCC
>Mature Secondary Structure
MLTVKQIEAAKPKEKPYRLLDGNGLYLYVPVSGKKVWQLRYKIDGKEKILTVGKYPLMTL
CCCHHHHHCCCCCCCCCEEECCCCEEEEEEECCCEEEEEEEEECCCCEEEEECCCCHHHH
QEARDKAWTARKDISVGIDPVKAKKASSNNNSFSAIYKEWYEHKKQVWSVGYATELAKMF
HHHHHHHHHHHCCCCCCCCCCHHCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
DDDILPIIGGLEIQDIEPMQLLEVIRRFEDRGAMERANKARRRCGEVFRYAIVTGRAKYN
CCCCHHHHCCEEECCCCHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHCCCCCCC
PAPDLADAMKGYRKKNFPFLPADQIPAFNKALATFSGSIVSLIATKVLRYTALRTKELRS
CCCCHHHHHHHHHCCCCCCCCCCCCCHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHH
MQWKSVDFENRIITIDASVMKGRKIHVVPMSDQVVELLTTLSSITKPVSEFVFAGRNDKK
HHCCCCCCCCEEEEEEHHHHCCCEEEEEECCHHHHHHHHHHHHHHHHHHHHHHCCCCCCC
KPICDNAVLLVIKQIGYEGLESGHGFRHEFSTIMNEHEWPADAIEVQLAHANGGSVRGIY
CCCCCHHHHHHHHHHCHHHHCCCCCHHHHHHHHHCCCCCCCCCEEEEEEECCCCCCCHHH
NHAQYLDKRREMMQWWADWLDEKVE
HHHHHHHHHHHHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 9205837; 9278503 [H]