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The map label for this gene is ackA

Identifier: 218700770

GI number: 218700770

Start: 2530755

End: 2531957

Strand: Direct

Name: ackA

Synonym: ECIAI39_2443

Alternate gene names: 218700770

Gene position: 2530755-2531957 (Clockwise)

Preceding gene: 218700765

Following gene: 218700771

Centisome position: 49.31

GC content: 52.2

Gene sequence:

>1203_bases
ATGTCGAGTAAGTTAGTACTGGTTCTGAACTGCGGTAGTTCTTCACTGAAATTTGCCATCATCGATGCAGTAAATGGTGA
AGAGTACCTTTCTGGTTTAGCCGAATGTTTCCACCTGCCCGAAGCACGTATCAAATGGAAAATGGACGGCAATAAACAGG
AAGCGGCTTTAGGTGCAGGCGCCGCTCACAGCGAAGCGCTCAACTTTATCGTTAATACTATTCTGGCACAAAAACCAGAA
CTGTCTGCGCAGTTGACTGCTATCGGTCACCGTATCGTACACGGCGGCGAAAAGTATACCAGCTCCGTAGTGATCGATGA
GTCTGTTATTCAGGGTATCAAAGATGCAGCTTCTTTTGCACCGCTGCACAACCCGGCTCACCTGATTGGTATCGAAGAAG
CTCTGAAATCTTTCCCACAGCTGAAAGACAAAAACGTTGCTGTATTTGACACCGCGTTCCACCAGACTATGCCGGAAGAG
TCTTACCTCTACGCCCTGCCGTACAACCTGTACAAAGAGCACGGCATCCGTCGTTACGGCGCGCACGGCACCAGCCACTT
CTATGTAACCCAGGAAGCGGCAAAAATGCTGAACAAACCGGTAGAAGAACTGAACATCATCACCTGCCACCTGGGCAACG
GTGGTTCCGTTTCTGCTATCCGCAACGGTAAATGCGTTGACACCTCTATGGGCCTGACCCCGCTGGAAGGTCTGGTCATG
GGTACCCGTTCTGGTGATATCGATCCGGCGATCATCTTCCACCTGCACGACACCCTGGGCATGAGCGTTGACGCAATCAA
CAAACTGCTGACCAAAGAGTCTGGCCTGCTGGGTCTGACCGAAGTGACCAGCGACTGCCGCTATGTTGAAGACAACTACG
CGACGAAAGAAGACGCGAAGCGCGCAATGGACGTTTACTGCCACCGCCTGGCGAAATACATCGGTGCCTACACTGCGCTG
ATGGATGGTCGTCTGGACGCTGTTGTATTCACCGGTGGTATCGGTGAAAATGCCGCAATGGTTCGTGAACTGTCTCTGGG
CAAACTGGGCGTGCTGGGCTTTGAAGTTGATCATGAACGCAACCTGGCTGCACGTTTCGGCAAATCTGGTTTCATCAACA
AAGAAGGTACCCGTCCTGCGGTGGTTATCCCAACCAACGAAGAACTGGTTATCGCGCAAGACGCGAGCCGCCTGACTGCC
TGA

Upstream 100 bases:

>100_bases
TGCAGTGCATGATGTTAATCATAAATGTCGGTGTCATCATGCGCTACGCTCTATGGCTCCCTGACGTTTTTTTAGCCACG
TATCAATTATAGGTACTTCC

Downstream 100 bases:

>100_bases
TTTCACACCGCCAGCTCAGCTGGCGGTGCTGTTTTGTAACCCGCCAAATCGGCGGTAACGAAAGAGGATAAACCGTGTCC
CGTATTATTATGCTGATCCC

Product: acetate kinase

Products: NA

Alternate protein names: Acetokinase

Number of amino acids: Translated: 400; Mature: 399

Protein sequence:

>400_residues
MSSKLVLVLNCGSSSLKFAIIDAVNGEEYLSGLAECFHLPEARIKWKMDGNKQEAALGAGAAHSEALNFIVNTILAQKPE
LSAQLTAIGHRIVHGGEKYTSSVVIDESVIQGIKDAASFAPLHNPAHLIGIEEALKSFPQLKDKNVAVFDTAFHQTMPEE
SYLYALPYNLYKEHGIRRYGAHGTSHFYVTQEAAKMLNKPVEELNIITCHLGNGGSVSAIRNGKCVDTSMGLTPLEGLVM
GTRSGDIDPAIIFHLHDTLGMSVDAINKLLTKESGLLGLTEVTSDCRYVEDNYATKEDAKRAMDVYCHRLAKYIGAYTAL
MDGRLDAVVFTGGIGENAAMVRELSLGKLGVLGFEVDHERNLAARFGKSGFINKEGTRPAVVIPTNEELVIAQDASRLTA

Sequences:

>Translated_400_residues
MSSKLVLVLNCGSSSLKFAIIDAVNGEEYLSGLAECFHLPEARIKWKMDGNKQEAALGAGAAHSEALNFIVNTILAQKPE
LSAQLTAIGHRIVHGGEKYTSSVVIDESVIQGIKDAASFAPLHNPAHLIGIEEALKSFPQLKDKNVAVFDTAFHQTMPEE
SYLYALPYNLYKEHGIRRYGAHGTSHFYVTQEAAKMLNKPVEELNIITCHLGNGGSVSAIRNGKCVDTSMGLTPLEGLVM
GTRSGDIDPAIIFHLHDTLGMSVDAINKLLTKESGLLGLTEVTSDCRYVEDNYATKEDAKRAMDVYCHRLAKYIGAYTAL
MDGRLDAVVFTGGIGENAAMVRELSLGKLGVLGFEVDHERNLAARFGKSGFINKEGTRPAVVIPTNEELVIAQDASRLTA
>Mature_399_residues
SSKLVLVLNCGSSSLKFAIIDAVNGEEYLSGLAECFHLPEARIKWKMDGNKQEAALGAGAAHSEALNFIVNTILAQKPEL
SAQLTAIGHRIVHGGEKYTSSVVIDESVIQGIKDAASFAPLHNPAHLIGIEEALKSFPQLKDKNVAVFDTAFHQTMPEES
YLYALPYNLYKEHGIRRYGAHGTSHFYVTQEAAKMLNKPVEELNIITCHLGNGGSVSAIRNGKCVDTSMGLTPLEGLVMG
TRSGDIDPAIIFHLHDTLGMSVDAINKLLTKESGLLGLTEVTSDCRYVEDNYATKEDAKRAMDVYCHRLAKYIGAYTALM
DGRLDAVVFTGGIGENAAMVRELSLGKLGVLGFEVDHERNLAARFGKSGFINKEGTRPAVVIPTNEELVIAQDASRLTA

Specific function: Involved In The Activation Of Acetate To Acetyl CoA And The Secretion Of Acetate. During Anaerobic Growth Of The Organism, This Enzyme Is Also Involved In The Synthesis Of Most Of The ATP Formed Catabolically. [C]

COG id: COG0282

COG function: function code C; Acetate kinase

Gene ontology:

Cell location: Cytoplasm

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the acetokinase family

Homologues:

Organism=Escherichia coli, GI1788633, Length=400, Percent_Identity=100, Blast_Score=824, Evalue=0.0,
Organism=Escherichia coli, GI145693184, Length=396, Percent_Identity=43.9393939393939, Blast_Score=315, Evalue=2e-87,

Paralogues:

None

Copy number: 1,800 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): ACKA_ECO57 (P0A6A5)

Other databases:

- EMBL:   AE005174
- EMBL:   BA000007
- PIR:   D91026
- RefSeq:   NP_288870.1
- RefSeq:   NP_311207.1
- PDB:   1LRG
- PDBsum:   1LRG
- ProteinModelPortal:   P0A6A5
- SMR:   P0A6A5
- EnsemblBacteria:   EBESCT00000028556
- EnsemblBacteria:   EBESCT00000060148
- GeneID:   916888
- GeneID:   958184
- GenomeReviews:   AE005174_GR
- GenomeReviews:   BA000007_GR
- KEGG:   ece:Z3558
- KEGG:   ecs:ECs3180
- GeneTree:   EBGT00050000009666
- HOGENOM:   HBG285863
- OMA:   PHLKDKN
- ProtClustDB:   PRK00180
- BioCyc:   ECOL83334:ECS3180-MONOMER
- GO:   GO:0005737
- HAMAP:   MF_00020
- InterPro:   IPR000890
- InterPro:   IPR004372
- PANTHER:   PTHR21060
- PANTHER:   PTHR21060:SF11
- PIRSF:   PIRSF000722
- PRINTS:   PR00471
- TIGRFAMs:   TIGR00016

Pfam domain/function: PF00871 Acetate_kinase

EC number: =2.7.2.1

Molecular weight: Translated: 43291; Mature: 43160

Theoretical pI: Translated: 6.23; Mature: 6.23

Prosite motif: PS01075 ACETATE_KINASE_1; PS01076 ACETATE_KINASE_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.5 %Cys     (Translated Protein)
2.5 %Met     (Translated Protein)
4.0 %Cys+Met (Translated Protein)
1.5 %Cys     (Mature Protein)
2.3 %Met     (Mature Protein)
3.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSSKLVLVLNCGSSSLKFAIIDAVNGEEYLSGLAECFHLPEARIKWKMDGNKQEAALGAG
CCCCEEEEEECCCCCEEEEEEEECCCHHHHHHHHHHHCCCCCEEEEEECCCCHHHHHCCC
AAHSEALNFIVNTILAQKPELSAQLTAIGHRIVHGGEKYTSSVVIDESVIQGIKDAASFA
CHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHCCCCHHHCEEEECHHHHHHHHHHHHHC
PLHNPAHLIGIEEALKSFPQLKDKNVAVFDTAFHQTMPEESYLYALPYNLYKEHGIRRYG
CCCCCHHEEEHHHHHHHCCCCCCCCEEEEEHHHHHCCCCCCEEEEECHHHHHHCCCEECC
AHGTSHFYVTQEAAKMLNKPVEELNIITCHLGNGGSVSAIRNGKCVDTSMGLTPLEGLVM
CCCCEEEEEEHHHHHHHHCCHHHCEEEEEEECCCCCEEEECCCEEEECCCCCCCCCCEEE
GTRSGDIDPAIIFHLHDTLGMSVDAINKLLTKESGLLGLTEVTSDCRYVEDNYATKEDAK
ECCCCCCCCEEEEEEHHHHCCCHHHHHHHHHHCCCCEEHHHHHCCCCCCCCCCCCHHHHH
RAMDVYCHRLAKYIGAYTALMDGRLDAVVFTGGIGENAAMVRELSLGKLGVLGFEVDHER
HHHHHHHHHHHHHHHHHHHHHCCCEEEEEEECCCCCCHHHHHHHCCCCEEEEEEEECCCC
NLAARFGKSGFINKEGTRPAVVIPTNEELVIAQDASRLTA
CHHHHCCCCCCCCCCCCCCEEEEECCCCEEEEECCHHCCC
>Mature Secondary Structure 
SSKLVLVLNCGSSSLKFAIIDAVNGEEYLSGLAECFHLPEARIKWKMDGNKQEAALGAG
CCCEEEEEECCCCCEEEEEEEECCCHHHHHHHHHHHCCCCCEEEEEECCCCHHHHHCCC
AAHSEALNFIVNTILAQKPELSAQLTAIGHRIVHGGEKYTSSVVIDESVIQGIKDAASFA
CHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHCCCCHHHCEEEECHHHHHHHHHHHHHC
PLHNPAHLIGIEEALKSFPQLKDKNVAVFDTAFHQTMPEESYLYALPYNLYKEHGIRRYG
CCCCCHHEEEHHHHHHHCCCCCCCCEEEEEHHHHHCCCCCCEEEEECHHHHHHCCCEECC
AHGTSHFYVTQEAAKMLNKPVEELNIITCHLGNGGSVSAIRNGKCVDTSMGLTPLEGLVM
CCCCEEEEEEHHHHHHHHCCHHHCEEEEEEECCCCCEEEECCCEEEECCCCCCCCCCEEE
GTRSGDIDPAIIFHLHDTLGMSVDAINKLLTKESGLLGLTEVTSDCRYVEDNYATKEDAK
ECCCCCCCCEEEEEEHHHHCCCHHHHHHHHHHCCCCEEHHHHHCCCCCCCCCCCCHHHHH
RAMDVYCHRLAKYIGAYTALMDGRLDAVVFTGGIGENAAMVRELSLGKLGVLGFEVDHER
HHHHHHHHHHHHHHHHHHHHHCCCEEEEEEECCCCCCHHHHHHHCCCCEEEEEEEECCCC
NLAARFGKSGFINKEGTRPAVVIPTNEELVIAQDASRLTA
CHHHHCCCCCCCCCCCCCCEEEEECCCCEEEEECCHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 11206551; 11258796