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The map label for this gene is yfbQ

Identifier: 218700764

GI number: 218700764

Start: 2524846

End: 2526063

Strand: Direct

Name: yfbQ

Synonym: ECIAI39_2437

Alternate gene names: 218700764

Gene position: 2524846-2526063 (Clockwise)

Preceding gene: 218700749

Following gene: 218700765

Centisome position: 49.2

GC content: 50.57

Gene sequence:

>1218_bases
ATGTCCCCCATTGAAAAATCCAGCAAATTAGAGAATGTCTGTTACGACATCCGTGGTCCGGTGCTGAAAGAAGCAAAACG
CCTGGAAGAAGAAGGTAACAAGGTACTGAAACTGAACATCGGCAACCCTGCCCCGTTCGGTTTTGACGCGCCAGATGAAA
TCCTCGTTGACGTGATTCGCAACCTGCCTACCGCTCAAGGGTATTGCGATTCCAAAGGGCTTTACTCCGCGCGTAAAGCC
ATCATGCAGCACTACCAGGCTCGTGGCATGCGTGATGTTACCGTGGAAGATATTTACATCGGCAATGGTGTATCGGAGCT
TATCGTTCAGGCAATGCAGGCATTGCTGAACAGCGGGGACGAAATGTTGGTTCCTGCACCTGATTACCCACTATGGACCG
CGGCGGTTTCGCTCTCCAGCGGTAAAGCGGTGCATTATCTTTGCGATGAATCCTCTGACTGGTTCCCGGACCTTGATGAT
ATTCGCGCTAAAATTACGCCTCGTACGCGTGGGATCGTTATTATCAACCCAAATAACCCAACTGGCGCGGTATATTCCAA
AGAGCTTTTAATGGAGATAGTGGAGATTGCACGTCAGCATAATCTCATTATCTTCGCCGATGAAATTTATGACAAAATTC
TCTACGACGACGCTGAGCATCACTCAATTGCGCCGCTGGCACCTGATCTGCTGACCATTACCTTTAACGGACTGTCGAAA
ACGTACCGCGTTGCAGGCTTCCGTCAGGGGTGGATGGTGTTGAACGGGCCGAAAAAACACGCCAAAGGCTACATCGAAGG
TCTGGAAATGCTGGCTTCAATGCGCCTGTGTGCTAACGTTCCTGCGCAACACGCCATTCAGACCGCGCTGGGCGGTTATC
AGAGCATCAGTGAATTTATTACCCCTGGCGGTCGTCTTTATGAGCAGCGTAACCGCGCATGGGAATTGATCAATGATATT
CCGGGCGTTTCCTGCGTGAAACCGCGCGGTGCGCTGTACATGTTCCCGAAAATCGACGCCAAACGCTTTAATATTCACGA
CGATCAGAAAATGGTGCTGGATTTCCTGTTGCAGGAAAAAGTTCTGTTGGTGCAAGGGACGGCATTCAACTGGCCGTGGC
CGGATCACTTCCGCATTGTCACGCTACCGCGTGTCGATGATATCGAGCTGTCTTTGAGCAAGTTCGCGCGTTTCCTTTCT
GGTTATCATCAGCTGTAA

Upstream 100 bases:

>100_bases
TATTCTGCAGACCTTCAAAACGCCGTCCTGGGGGAGTACATTGTTCTAAGCTGACTTCCACGGCAGGGAGTGGCGATAAC
AGCAAAAAAGGTCAAGATTC

Downstream 100 bases:

>100_bases
TCTTAATTTCACTGCCGGAGATTGCATCCGGCAGTGTTATCCCGCCACAATGACCTGATGATGTCATCATACGTAAGGTC
ACTATGAAACAGAGCCATTT

Product: aminotransferase AlaT

Products: oxaloacetate; L-glutamate

Alternate protein names: NA

Number of amino acids: Translated: 405; Mature: 404

Protein sequence:

>405_residues
MSPIEKSSKLENVCYDIRGPVLKEAKRLEEEGNKVLKLNIGNPAPFGFDAPDEILVDVIRNLPTAQGYCDSKGLYSARKA
IMQHYQARGMRDVTVEDIYIGNGVSELIVQAMQALLNSGDEMLVPAPDYPLWTAAVSLSSGKAVHYLCDESSDWFPDLDD
IRAKITPRTRGIVIINPNNPTGAVYSKELLMEIVEIARQHNLIIFADEIYDKILYDDAEHHSIAPLAPDLLTITFNGLSK
TYRVAGFRQGWMVLNGPKKHAKGYIEGLEMLASMRLCANVPAQHAIQTALGGYQSISEFITPGGRLYEQRNRAWELINDI
PGVSCVKPRGALYMFPKIDAKRFNIHDDQKMVLDFLLQEKVLLVQGTAFNWPWPDHFRIVTLPRVDDIELSLSKFARFLS
GYHQL

Sequences:

>Translated_405_residues
MSPIEKSSKLENVCYDIRGPVLKEAKRLEEEGNKVLKLNIGNPAPFGFDAPDEILVDVIRNLPTAQGYCDSKGLYSARKA
IMQHYQARGMRDVTVEDIYIGNGVSELIVQAMQALLNSGDEMLVPAPDYPLWTAAVSLSSGKAVHYLCDESSDWFPDLDD
IRAKITPRTRGIVIINPNNPTGAVYSKELLMEIVEIARQHNLIIFADEIYDKILYDDAEHHSIAPLAPDLLTITFNGLSK
TYRVAGFRQGWMVLNGPKKHAKGYIEGLEMLASMRLCANVPAQHAIQTALGGYQSISEFITPGGRLYEQRNRAWELINDI
PGVSCVKPRGALYMFPKIDAKRFNIHDDQKMVLDFLLQEKVLLVQGTAFNWPWPDHFRIVTLPRVDDIELSLSKFARFLS
GYHQL
>Mature_404_residues
SPIEKSSKLENVCYDIRGPVLKEAKRLEEEGNKVLKLNIGNPAPFGFDAPDEILVDVIRNLPTAQGYCDSKGLYSARKAI
MQHYQARGMRDVTVEDIYIGNGVSELIVQAMQALLNSGDEMLVPAPDYPLWTAAVSLSSGKAVHYLCDESSDWFPDLDDI
RAKITPRTRGIVIINPNNPTGAVYSKELLMEIVEIARQHNLIIFADEIYDKILYDDAEHHSIAPLAPDLLTITFNGLSKT
YRVAGFRQGWMVLNGPKKHAKGYIEGLEMLASMRLCANVPAQHAIQTALGGYQSISEFITPGGRLYEQRNRAWELINDIP
GVSCVKPRGALYMFPKIDAKRFNIHDDQKMVLDFLLQEKVLLVQGTAFNWPWPDHFRIVTLPRVDDIELSLSKFARFLSG
YHQL

Specific function: Unknown

COG id: COG0436

COG function: function code E; Aspartate/tyrosine/aromatic aminotransferase

Gene ontology:

Cell location: Cytoplasm

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the class-I pyridoxal-phosphate-dependent aminotransferase family

Homologues:

Organism=Homo sapiens, GI4507369, Length=382, Percent_Identity=26.4397905759162, Blast_Score=131, Evalue=1e-30,
Organism=Homo sapiens, GI19263340, Length=462, Percent_Identity=25.3246753246753, Blast_Score=127, Evalue=2e-29,
Organism=Homo sapiens, GI215599424, Length=368, Percent_Identity=26.6304347826087, Blast_Score=125, Evalue=8e-29,
Organism=Homo sapiens, GI4885351, Length=447, Percent_Identity=23.7136465324385, Blast_Score=114, Evalue=1e-25,
Organism=Homo sapiens, GI95147551, Length=171, Percent_Identity=28.6549707602339, Blast_Score=90, Evalue=4e-18,
Organism=Homo sapiens, GI169881279, Length=171, Percent_Identity=28.6549707602339, Blast_Score=90, Evalue=4e-18,
Organism=Homo sapiens, GI56713256, Length=235, Percent_Identity=26.3829787234043, Blast_Score=86, Evalue=5e-17,
Organism=Homo sapiens, GI56713254, Length=235, Percent_Identity=26.3829787234043, Blast_Score=86, Evalue=6e-17,
Organism=Homo sapiens, GI169881281, Length=161, Percent_Identity=30.4347826086957, Blast_Score=82, Evalue=1e-15,
Organism=Homo sapiens, GI149944424, Length=335, Percent_Identity=21.4925373134328, Blast_Score=72, Evalue=8e-13,
Organism=Escherichia coli, GI1788627, Length=405, Percent_Identity=100, Blast_Score=840, Evalue=0.0,
Organism=Escherichia coli, GI1788722, Length=376, Percent_Identity=26.063829787234, Blast_Score=97, Evalue=2e-21,
Organism=Escherichia coli, GI1786816, Length=263, Percent_Identity=25.0950570342205, Blast_Score=78, Evalue=8e-16,
Organism=Caenorhabditis elegans, GI17567663, Length=369, Percent_Identity=29.2682926829268, Blast_Score=159, Evalue=2e-39,
Organism=Caenorhabditis elegans, GI71981209, Length=458, Percent_Identity=25.764192139738, Blast_Score=111, Evalue=6e-25,
Organism=Caenorhabditis elegans, GI71994476, Length=205, Percent_Identity=29.7560975609756, Blast_Score=91, Evalue=9e-19,
Organism=Caenorhabditis elegans, GI71994472, Length=205, Percent_Identity=29.7560975609756, Blast_Score=91, Evalue=1e-18,
Organism=Caenorhabditis elegans, GI71981215, Length=293, Percent_Identity=26.6211604095563, Blast_Score=88, Evalue=6e-18,
Organism=Caenorhabditis elegans, GI17567369, Length=178, Percent_Identity=29.2134831460674, Blast_Score=84, Evalue=1e-16,
Organism=Saccharomyces cerevisiae, GI6320317, Length=360, Percent_Identity=27.5, Blast_Score=131, Evalue=2e-31,
Organism=Saccharomyces cerevisiae, GI6323118, Length=357, Percent_Identity=27.7310924369748, Blast_Score=126, Evalue=7e-30,
Organism=Saccharomyces cerevisiae, GI6322401, Length=303, Percent_Identity=24.0924092409241, Blast_Score=105, Evalue=1e-23,
Organism=Drosophila melanogaster, GI18859735, Length=375, Percent_Identity=26.6666666666667, Blast_Score=149, Evalue=5e-36,
Organism=Drosophila melanogaster, GI24641770, Length=470, Percent_Identity=26.8085106382979, Blast_Score=121, Evalue=9e-28,
Organism=Drosophila melanogaster, GI24641760, Length=470, Percent_Identity=26.8085106382979, Blast_Score=121, Evalue=9e-28,
Organism=Drosophila melanogaster, GI24641768, Length=470, Percent_Identity=26.8085106382979, Blast_Score=121, Evalue=9e-28,
Organism=Drosophila melanogaster, GI24641766, Length=470, Percent_Identity=26.8085106382979, Blast_Score=121, Evalue=9e-28,
Organism=Drosophila melanogaster, GI24641764, Length=470, Percent_Identity=26.8085106382979, Blast_Score=121, Evalue=9e-28,
Organism=Drosophila melanogaster, GI45551451, Length=470, Percent_Identity=26.8085106382979, Blast_Score=121, Evalue=1e-27,
Organism=Drosophila melanogaster, GI28573069, Length=167, Percent_Identity=27.5449101796407, Blast_Score=82, Evalue=9e-16,
Organism=Drosophila melanogaster, GI24646114, Length=167, Percent_Identity=27.5449101796407, Blast_Score=82, Evalue=9e-16,
Organism=Drosophila melanogaster, GI28573067, Length=167, Percent_Identity=27.5449101796407, Blast_Score=82, Evalue=9e-16,
Organism=Drosophila melanogaster, GI28573065, Length=167, Percent_Identity=27.5449101796407, Blast_Score=82, Evalue=9e-16,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): YFBQ_ECOL6 (P0A960)

Other databases:

- EMBL:   AE014075
- RefSeq:   NP_754717.1
- ProteinModelPortal:   P0A960
- SMR:   P0A960
- EnsemblBacteria:   EBESCT00000042841
- GeneID:   1038373
- GenomeReviews:   AE014075_GR
- KEGG:   ecc:c2831
- GeneTree:   EBGT00050000008899
- HOGENOM:   HBG645860
- OMA:   MFPKIDT
- ProtClustDB:   PRK09265
- GO:   GO:0005737
- InterPro:   IPR001176
- InterPro:   IPR004839
- InterPro:   IPR015424
- InterPro:   IPR015421
- InterPro:   IPR015422
- Gene3D:   G3DSA:3.40.640.10
- Gene3D:   G3DSA:3.90.1150.10
- PRINTS:   PR00753

Pfam domain/function: PF00155 Aminotran_1_2; SSF53383 PyrdxlP-dep_Trfase_major

EC number: 2.6.1.1

Molecular weight: Translated: 45518; Mature: 45387

Theoretical pI: Translated: 6.19; Mature: 6.19

Prosite motif: PS00105 AA_TRANSFER_CLASS_1

Important sites: BINDING 41-41 BINDING 179-179 BINDING 378-378

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.2 %Cys     (Translated Protein)
2.7 %Met     (Translated Protein)
4.0 %Cys+Met (Translated Protein)
1.2 %Cys     (Mature Protein)
2.5 %Met     (Mature Protein)
3.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSPIEKSSKLENVCYDIRGPVLKEAKRLEEEGNKVLKLNIGNPAPFGFDAPDEILVDVIR
CCCCCHHHHHHHHHHHHCCHHHHHHHHHHHCCCEEEEEECCCCCCCCCCCHHHHHHHHHH
NLPTAQGYCDSKGLYSARKAIMQHYQARGMRDVTVEDIYIGNGVSELIVQAMQALLNSGD
CCCCCCCCCCCCCHHHHHHHHHHHHHHCCCCCCEEEEEEECCCHHHHHHHHHHHHHCCCC
EMLVPAPDYPLWTAAVSLSSGKAVHYLCDESSDWFPDLDDIRAKITPRTRGIVIINPNNP
CEEEECCCCCHHHHEEECCCCCEEEEEECCCCCCCCCHHHHHEEECCCCCEEEEECCCCC
TGAVYSKELLMEIVEIARQHNLIIFADEIYDKILYDDAEHHSIAPLAPDLLTITFNGLSK
CCHHHHHHHHHHHHHHHHHCCEEEEEHHHHHHHHCCCCCCCCCCCCCCCEEEEEECCCCH
TYRVAGFRQGWMVLNGPKKHAKGYIEGLEMLASMRLCANVPAQHAIQTALGGYQSISEFI
HHHHCCCCCCCEEEECCHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHCCHHHHHHHH
TPGGRLYEQRNRAWELINDIPGVSCVKPRGALYMFPKIDAKRFNIHDDQKMVLDFLLQEK
CCCHHHHHHHHHHHHHHHHCCCCEEECCCCCEEEEECCCCCCCCCCCHHHHHHHHHHHCC
VLLVQGTAFNWPWPDHFRIVTLPRVDDIELSLSKFARFLSGYHQL
EEEEECCCCCCCCCCCEEEEECCCCCCHHHHHHHHHHHHHHHHCC
>Mature Secondary Structure 
SPIEKSSKLENVCYDIRGPVLKEAKRLEEEGNKVLKLNIGNPAPFGFDAPDEILVDVIR
CCCCHHHHHHHHHHHHCCHHHHHHHHHHHCCCEEEEEECCCCCCCCCCCHHHHHHHHHH
NLPTAQGYCDSKGLYSARKAIMQHYQARGMRDVTVEDIYIGNGVSELIVQAMQALLNSGD
CCCCCCCCCCCCCHHHHHHHHHHHHHHCCCCCCEEEEEEECCCHHHHHHHHHHHHHCCCC
EMLVPAPDYPLWTAAVSLSSGKAVHYLCDESSDWFPDLDDIRAKITPRTRGIVIINPNNP
CEEEECCCCCHHHHEEECCCCCEEEEEECCCCCCCCCHHHHHEEECCCCCEEEEECCCCC
TGAVYSKELLMEIVEIARQHNLIIFADEIYDKILYDDAEHHSIAPLAPDLLTITFNGLSK
CCHHHHHHHHHHHHHHHHHCCEEEEEHHHHHHHHCCCCCCCCCCCCCCCEEEEEECCCCH
TYRVAGFRQGWMVLNGPKKHAKGYIEGLEMLASMRLCANVPAQHAIQTALGGYQSISEFI
HHHHCCCCCCCEEEECCHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHCCHHHHHHHH
TPGGRLYEQRNRAWELINDIPGVSCVKPRGALYMFPKIDAKRFNIHDDQKMVLDFLLQEK
CCCHHHHHHHHHHHHHHHHCCCCEEECCCCCEEEEECCCCCCCCCCCHHHHHHHHHHHCC
VLLVQGTAFNWPWPDHFRIVTLPRVDDIELSLSKFARFLSGYHQL
EEEEECCCCCCCCCCCEEEEECCCCCCHHHHHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: L-aspartate; 2-oxoglutarate

Specific reaction: L-aspartate + 2-oxoglutarate = oxaloacetate + L-glutamate

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 12471157