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The map label for this gene is arnT

Identifier: 218700731

GI number: 218700731

Start: 2489922

End: 2491574

Strand: Direct

Name: arnT

Synonym: ECIAI39_2404

Alternate gene names: 218700731

Gene position: 2489922-2491574 (Clockwise)

Preceding gene: 218700730

Following gene: 218700732

Centisome position: 48.52

GC content: 50.94

Gene sequence:

>1653_bases
ATGAAATCGGTACGTTACCTTATCGGCCTCTTCGCGTTTATTGCCTGCTATTACCTGTTACCGATCAGCACGCGTCTGCT
CTGGCAACCCGATGAAACGCGTTATGCGGAAATCAGTCGAGAAATGCTGGCATCCGGCGACTGGATTGTGCCCCATCTGT
TAGGGCTACGTTATTTCGAAAAACCCATTGCCGGATACTGGATTAACAGCATTGGGCAATGGCTATTTGGCGCGAATAAC
TTTGGTGTGCGGGCAGGCGTTATCTTTGCGACCCTGTTAACTGCCGCGCTGGTGACCTGGTTTACTCTGCGCTTATGGCG
CGATAAACGTCTGGCTCTACTCGCCACAGTAATTTATCTCTCATTGTTTATTGTCTATGCCATCGGCACTTATGCCGTGC
TCGATCCGTTTATTGCATTCTGGCTGGTGGCGGGAATGTGCAGCTTCTGGCTGGCAATGCAGGCACAGACGTGGAAAGGC
AAAAGCGCAGGATTTTTACTGCTGGGAATCACCTGCGGCATGGGGGTGATGACCAAAGGTTTTCTCGCCCTTGCCGTGCC
GGTATTAAGCGTGCTGCCATGGGTAGCGACGCAAAAACGCTGGAAAGATCTCTTTATTTACGGCTGGCTGGCGGTTATCA
GTTGCGTACTGACGGTTCTCCCCTGGGGACTGGCGATAGCGCAGCGGGAGCCTGACTTCTGGCACTATTTTTTCTGGGTT
GAGCATATTCAACGCTTTGCACTGGATGATGCCCAACATAGAGCTCCGTTCTGGTACTACGTGCCGGTCATCATTGCCGG
TAGCCTGCCGTGGCTGGGATTACTCCCCGGTGCACTGTACACAGGCTGGAAAAACCGCAAGCATTCCGCAACCGTCTATT
TGTTGAGCTGGACGATAATGCCGCTGCTGTTTTTCTCCGTCGCTAAAGGTAAATTGCCCACCTATATTCTTTCCTGCTTT
GCATCTCTGGCAATGCTGATGGCGCATTACGCTTTGCTGGCAGCAAAAAATAATCCTCTGGCGCTGCGGATTAATGGCTG
GATTAACATCGCTTTTGGTGTCACTGGCATTATTGCTACGTTTGTGGTCTCCCCATGGGGACCAATGAACACGCCAGTGT
GGCAAACCTTCGAGAGCTATAAAGTCTTTTGTGCCTGGTCGATTTTTTCGCTATGGGCATTTTTCGGCTGGTACACCTTA
ACAAACGTCGAAAAGACCTGGTCTTTTGCCGCGCTTTGCCCGCTGGGGCTGGCGTTGCTGGTAGGATTTTCAATTCCTGA
CAGAGTCATGGAAGGAAAACATCCGCAATTTTTTGTCGAGATGACACAAGAATCACTTCAGCCAAGCCGCTATATTCTTA
CTGATAGCGTCGGTGTTGCCGCAGGTCTGGCATGGAGCCTGCAACGCGATGACATCATCATGTATCGCCAGACAGGTGAG
TTGAAATACGGCCTTAATTATCCGGATGCGAAAGGGAGATTTGTCAGCGGTGATGAGTTCGCAAACTGGCTTAATCAACA
TCGTCAGGAGGGGATTATTACACTCGTACTTTCGGTTGACCGCGATGAAGATATCAACAGTCTCGCCATCCCGCCCGCAG
ATGCCATCGATCGTCAGGAGCGTCTGGTGCTGATTCAGTATCGTCCCAAATGA

Upstream 100 bases:

>100_bases
AACTGTTATCAGAGACGTTGCCGCTCGGACAAGTTGTTCGCGGAAATATTGCCGGACGTGAAGGCTGGCTGGGTTGCCAA
CAAATTGCGGGTAGTCGCTG

Downstream 100 bases:

>100_bases
TCTGGCTAACATTAGTCTTTGCCAGCTTGCTTAGCGTTGCCGGGCAGTTGTGTCAGAAACAGGCAACCTGCTTTGTGGCG
ATAAACAAACGGCGCAAACA

Product: 4-amino-4-deoxy-L-arabinose transferase

Products: NA

Alternate protein names: 4-amino-4-deoxy-L-arabinose lipid A transferase; Lipid IV(A) 4-amino-4-deoxy-L-arabinosyltransferase; Undecaprenyl phosphate-alpha-L-Ara4N transferase [H]

Number of amino acids: Translated: 550; Mature: 550

Protein sequence:

>550_residues
MKSVRYLIGLFAFIACYYLLPISTRLLWQPDETRYAEISREMLASGDWIVPHLLGLRYFEKPIAGYWINSIGQWLFGANN
FGVRAGVIFATLLTAALVTWFTLRLWRDKRLALLATVIYLSLFIVYAIGTYAVLDPFIAFWLVAGMCSFWLAMQAQTWKG
KSAGFLLLGITCGMGVMTKGFLALAVPVLSVLPWVATQKRWKDLFIYGWLAVISCVLTVLPWGLAIAQREPDFWHYFFWV
EHIQRFALDDAQHRAPFWYYVPVIIAGSLPWLGLLPGALYTGWKNRKHSATVYLLSWTIMPLLFFSVAKGKLPTYILSCF
ASLAMLMAHYALLAAKNNPLALRINGWINIAFGVTGIIATFVVSPWGPMNTPVWQTFESYKVFCAWSIFSLWAFFGWYTL
TNVEKTWSFAALCPLGLALLVGFSIPDRVMEGKHPQFFVEMTQESLQPSRYILTDSVGVAAGLAWSLQRDDIIMYRQTGE
LKYGLNYPDAKGRFVSGDEFANWLNQHRQEGIITLVLSVDRDEDINSLAIPPADAIDRQERLVLIQYRPK

Sequences:

>Translated_550_residues
MKSVRYLIGLFAFIACYYLLPISTRLLWQPDETRYAEISREMLASGDWIVPHLLGLRYFEKPIAGYWINSIGQWLFGANN
FGVRAGVIFATLLTAALVTWFTLRLWRDKRLALLATVIYLSLFIVYAIGTYAVLDPFIAFWLVAGMCSFWLAMQAQTWKG
KSAGFLLLGITCGMGVMTKGFLALAVPVLSVLPWVATQKRWKDLFIYGWLAVISCVLTVLPWGLAIAQREPDFWHYFFWV
EHIQRFALDDAQHRAPFWYYVPVIIAGSLPWLGLLPGALYTGWKNRKHSATVYLLSWTIMPLLFFSVAKGKLPTYILSCF
ASLAMLMAHYALLAAKNNPLALRINGWINIAFGVTGIIATFVVSPWGPMNTPVWQTFESYKVFCAWSIFSLWAFFGWYTL
TNVEKTWSFAALCPLGLALLVGFSIPDRVMEGKHPQFFVEMTQESLQPSRYILTDSVGVAAGLAWSLQRDDIIMYRQTGE
LKYGLNYPDAKGRFVSGDEFANWLNQHRQEGIITLVLSVDRDEDINSLAIPPADAIDRQERLVLIQYRPK
>Mature_550_residues
MKSVRYLIGLFAFIACYYLLPISTRLLWQPDETRYAEISREMLASGDWIVPHLLGLRYFEKPIAGYWINSIGQWLFGANN
FGVRAGVIFATLLTAALVTWFTLRLWRDKRLALLATVIYLSLFIVYAIGTYAVLDPFIAFWLVAGMCSFWLAMQAQTWKG
KSAGFLLLGITCGMGVMTKGFLALAVPVLSVLPWVATQKRWKDLFIYGWLAVISCVLTVLPWGLAIAQREPDFWHYFFWV
EHIQRFALDDAQHRAPFWYYVPVIIAGSLPWLGLLPGALYTGWKNRKHSATVYLLSWTIMPLLFFSVAKGKLPTYILSCF
ASLAMLMAHYALLAAKNNPLALRINGWINIAFGVTGIIATFVVSPWGPMNTPVWQTFESYKVFCAWSIFSLWAFFGWYTL
TNVEKTWSFAALCPLGLALLVGFSIPDRVMEGKHPQFFVEMTQESLQPSRYILTDSVGVAAGLAWSLQRDDIIMYRQTGE
LKYGLNYPDAKGRFVSGDEFANWLNQHRQEGIITLVLSVDRDEDINSLAIPPADAIDRQERLVLIQYRPK

Specific function: Catalyzes the transfer of the L-Ara4N moiety of the glycolipid undecaprenyl phosphate-alpha-L-Ara4N to lipid A. The modified arabinose is attached to lipid A and is required for resistance to polymyxin and cationic antimicrobial peptides [H]

COG id: COG1807

COG function: function code M; 4-amino-4-deoxy-L-arabinose transferase and related glycosyltransferases of PMT family

Gene ontology:

Cell location: Cell inner membrane; Multi-pass membrane protein [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the glycosyltransferase 83 family [H]

Homologues:

Organism=Escherichia coli, GI1788591, Length=550, Percent_Identity=99.6363636363636, Blast_Score=1112, Evalue=0.0,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR022839
- InterPro:   IPR003342 [H]

Pfam domain/function: PF02366 PMT [H]

EC number: =2.4.2.43 [H]

Molecular weight: Translated: 62534; Mature: 62534

Theoretical pI: Translated: 9.11; Mature: 9.11

Prosite motif: PS00013 PROKAR_LIPOPROTEIN

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.3 %Cys     (Translated Protein)
2.4 %Met     (Translated Protein)
3.6 %Cys+Met (Translated Protein)
1.3 %Cys     (Mature Protein)
2.4 %Met     (Mature Protein)
3.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure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CEEEEEECCC
>Mature Secondary Structure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CEEEEEECCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: NA