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The map label for this gene is yfaO

Identifier: 218700725

GI number: 218700725

Start: 2483568

End: 2483993

Strand: Direct

Name: yfaO

Synonym: ECIAI39_2398

Alternate gene names: 218700725

Gene position: 2483568-2483993 (Clockwise)

Preceding gene: 218700721

Following gene: 218700727

Centisome position: 48.39

GC content: 48.12

Gene sequence:

>426_bases
GTGCGACAACGGACTATTGTATGCCCTTTGATTCAAAATGATGGTGCTTATTTGCTGTGTAAAATGGCCGACGATCGCGG
CGTTTTCCCCGGTCAATGGGCGCTTTCGGGTGGCGGCGTAGAGCCTGGCGAACGAATTGAAGAGGCACTACGCCGCGAAA
TTCGCGAAGAACTGGGAGAACAGCTGCTTTTGACAGAAATCACGCCGTGGACCTTCAGCGATGATATTCGCACCAAGACG
TATGCAGATGGTCGCAAGGAAGAGATTTATATGATTTACCTGATTTTTGACTGCGTTTCTGCCAACCGAGAAGTGAAAAT
AAACGAAGAGTTTCAGGACTACGCGTGGGTAAAACCTGAAGATCTGGCGCACTATGATTTGAATGTCGCCACCCGAAAAA
CGTTACGTTTGAAAGGTCTTCTGTAA

Upstream 100 bases:

>100_bases
ATAAGTACAGTTATCATCGCAAAAGAGATTACTGTCACTTTCACATATTTCTGAGTTACCGTATTCTCATGGTTTCTTTT
TATTTATAAGGAATACCCCT

Downstream 100 bases:

>100_bases
CAATGATGACAGCCATTACTGGCTGTCAGATTGAAAATTTAGTGGTTAACGAATTCCCCATCCAGATAAACTTTGCCTTT
TTCCACATGCATGACTAAAC

Product: putative NUDIX hydrolase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 141; Mature: 141

Protein sequence:

>141_residues
MRQRTIVCPLIQNDGAYLLCKMADDRGVFPGQWALSGGGVEPGERIEEALRREIREELGEQLLLTEITPWTFSDDIRTKT
YADGRKEEIYMIYLIFDCVSANREVKINEEFQDYAWVKPEDLAHYDLNVATRKTLRLKGLL

Sequences:

>Translated_141_residues
MRQRTIVCPLIQNDGAYLLCKMADDRGVFPGQWALSGGGVEPGERIEEALRREIREELGEQLLLTEITPWTFSDDIRTKT
YADGRKEEIYMIYLIFDCVSANREVKINEEFQDYAWVKPEDLAHYDLNVATRKTLRLKGLL
>Mature_141_residues
MRQRTIVCPLIQNDGAYLLCKMADDRGVFPGQWALSGGGVEPGERIEEALRREIREELGEQLLLTEITPWTFSDDIRTKT
YADGRKEEIYMIYLIFDCVSANREVKINEEFQDYAWVKPEDLAHYDLNVATRKTLRLKGLL

Specific function: Catalyzes the hydrolysis of nucleoside triphosphates, with a preference for pyrimidine deoxynucleoside triphosphates (dUTP, dTTP and dCTP) [H]

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 nudix hydrolase domain [H]

Homologues:

Organism=Escherichia coli, GI1788585, Length=141, Percent_Identity=98.5815602836879, Blast_Score=289, Evalue=6e-80,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR020476
- InterPro:   IPR020084
- InterPro:   IPR000086
- InterPro:   IPR015797 [H]

Pfam domain/function: PF00293 NUDIX [H]

EC number: 3.6.-.- [C]

Molecular weight: Translated: 16343; Mature: 16343

Theoretical pI: Translated: 4.64; Mature: 4.64

Prosite motif: PS00893 NUDIX

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.1 %Cys     (Translated Protein)
2.1 %Met     (Translated Protein)
4.3 %Cys+Met (Translated Protein)
2.1 %Cys     (Mature Protein)
2.1 %Met     (Mature Protein)
4.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MRQRTIVCPLIQNDGAYLLCKMADDRGVFPGQWALSGGGVEPGERIEEALRREIREELGE
CCCCEEEEEEEECCCCEEEEEECCCCCCCCCCEEECCCCCCHHHHHHHHHHHHHHHHHCC
QLLLTEITPWTFSDDIRTKTYADGRKEEIYMIYLIFDCVSANREVKINEEFQDYAWVKPE
EEEEEECCCCCCCCCCCCCCCCCCCCCEEEEEEEEEEHHCCCCEEEECCCCCCCCCCCCC
DLAHYDLNVATRKTLRLKGLL
CCCEECCCHHHCEEEEEECCC
>Mature Secondary Structure
MRQRTIVCPLIQNDGAYLLCKMADDRGVFPGQWALSGGGVEPGERIEEALRREIREELGE
CCCCEEEEEEEECCCCEEEEEECCCCCCCCCCEEECCCCCCHHHHHHHHHHHHHHHHHCC
QLLLTEITPWTFSDDIRTKTYADGRKEEIYMIYLIFDCVSANREVKINEEFQDYAWVKPE
EEEEEECCCCCCCCCCCCCCCCCCCCCEEEEEEEEEEHHCCCCEEEECCCCCCCCCCCCC
DLAHYDLNVATRKTLRLKGLL
CCCEECCCHHHCEEEEEECCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA