Click here to switch to the map view.

The map label for this gene is atoB

Identifier: 218700693

GI number: 218700693

Start: 2438200

End: 2439384

Strand: Direct

Name: atoB

Synonym: ECIAI39_2362

Alternate gene names: 218700693

Gene position: 2438200-2439384 (Clockwise)

Preceding gene: 218700692

Following gene: 218700701

Centisome position: 47.51

GC content: 53.25

Gene sequence:

>1185_bases
ATGAAAAATTGTGTCATCGTCAGTGCGGTACGTACTGCTATCGGTAGTTTTAACGGTTCACTCGCTTCCACCAGCGCCAT
CGACCTGGGGGCGACAGTAATTAAAGCCGCCATTGAACGTGCAAAAATCGATTCACTACACATTGATGAAGTGATTATGG
GTAACGTGTTGCAAGCCGGGCTGGGGCAAAATCCGGCGCGTCAGGCACTGTTAAAAAGCGGGCTGGCAGAAACGGTGTGC
GGATTCACGGTCAATAAAGTGTGTGGTTCGGGTCTTAAAAGTGTGGCGCTTGCCGCCCAGGCAATTCAGGCAGGTCAGGC
GCAAAGCATTGTGGCGGGGGGTATGGAAAATATGAGTTTAGCGCCCTACTTACTCGATGCAAAAGCACGCTCTGGTTATC
GTCTTGGAGACGGACAGGTTTATGACGTAATCCTGCGCGATGGTCTGATGTGCGCCACCCATGGTTATCATATGGGGATT
ACCGCCGAGAACGTGGCCAAAGAGTACGGAATTACCCGAGAAATGCAGGATGAACTGGCGCTACATTCACAGCGTAAAGC
GGCAGCCGCAATTGAGTCCGGTGCTTTTACAGCCGAAATCGTCCCGGTAAATGTTGTCACTCGGAAGAAAACCTTCGTCT
TCAGTCAAGACGAATTCCCGAAAGCGGATTCTACGACTGAAGCGTTAGGCGCATTGCGTCCGGCCTTCGATAAAGCAGGA
ACAGTCACCGCCGGGAACGCGTCTGGTATTAACGACGGTGCTGCCGCTCTGGTGATTATGGAAGAATCTGCGGCGCTGGC
AGCAGGCCTTACCCCCCTGGCTCGCATTAAAAGTTATGCCAGCGGTGGCGTGCCCCCCGCATTGATGGGTATGGGGCCAG
TACCTGCCACGCAAAAAGCGTTACAACTGGCGGGGCTGCAACTGGCGGATATTGATCTCATTGAGGCTAATGAAGCATTT
GCCGCACAGTTCCTTGCCGTTGGGAAAACCCTGGGCTTTGATTCTGAGAAAGTGAATGTCAACGGCGGGGCCATCGCGCT
CGGGCATCCTATCGGTGCCAGTGGTGCTCGTATTCTGGTCACACTATTACATGCCATGCAGGCACGCGATAAAACGCTGG
GGCTGGCAACACTGTGCATTGGTGGTGGCCAGGGAATTGCGATGGTGATTGAACGGTTGAATTAA

Upstream 100 bases:

>100_bases
CATGGGCTATTGCATCACTGCCCTGCTCTTCTCCGGCGTCATTTTCGTCATTGGTTTAACGCTGTTCTGACGGCAACCCT
ACAGACAGAAGGAATATAAA

Downstream 100 bases:

>100_bases
CCAATAAAAACACCCGATAGCGAAAGTTATCGGGCGTTTTCTTGAACATCGACGGCGAAGGTAACCCCATTAATCACCAG
TCAAAACTTTTCACCAGCGT

Product: acetyl-CoA acetyltransferase

Products: NA

Alternate protein names: Acetoacetyl-CoA thiolase [H]

Number of amino acids: Translated: 394; Mature: 394

Protein sequence:

>394_residues
MKNCVIVSAVRTAIGSFNGSLASTSAIDLGATVIKAAIERAKIDSLHIDEVIMGNVLQAGLGQNPARQALLKSGLAETVC
GFTVNKVCGSGLKSVALAAQAIQAGQAQSIVAGGMENMSLAPYLLDAKARSGYRLGDGQVYDVILRDGLMCATHGYHMGI
TAENVAKEYGITREMQDELALHSQRKAAAAIESGAFTAEIVPVNVVTRKKTFVFSQDEFPKADSTTEALGALRPAFDKAG
TVTAGNASGINDGAAALVIMEESAALAAGLTPLARIKSYASGGVPPALMGMGPVPATQKALQLAGLQLADIDLIEANEAF
AAQFLAVGKTLGFDSEKVNVNGGAIALGHPIGASGARILVTLLHAMQARDKTLGLATLCIGGGQGIAMVIERLN

Sequences:

>Translated_394_residues
MKNCVIVSAVRTAIGSFNGSLASTSAIDLGATVIKAAIERAKIDSLHIDEVIMGNVLQAGLGQNPARQALLKSGLAETVC
GFTVNKVCGSGLKSVALAAQAIQAGQAQSIVAGGMENMSLAPYLLDAKARSGYRLGDGQVYDVILRDGLMCATHGYHMGI
TAENVAKEYGITREMQDELALHSQRKAAAAIESGAFTAEIVPVNVVTRKKTFVFSQDEFPKADSTTEALGALRPAFDKAG
TVTAGNASGINDGAAALVIMEESAALAAGLTPLARIKSYASGGVPPALMGMGPVPATQKALQLAGLQLADIDLIEANEAF
AAQFLAVGKTLGFDSEKVNVNGGAIALGHPIGASGARILVTLLHAMQARDKTLGLATLCIGGGQGIAMVIERLN
>Mature_394_residues
MKNCVIVSAVRTAIGSFNGSLASTSAIDLGATVIKAAIERAKIDSLHIDEVIMGNVLQAGLGQNPARQALLKSGLAETVC
GFTVNKVCGSGLKSVALAAQAIQAGQAQSIVAGGMENMSLAPYLLDAKARSGYRLGDGQVYDVILRDGLMCATHGYHMGI
TAENVAKEYGITREMQDELALHSQRKAAAAIESGAFTAEIVPVNVVTRKKTFVFSQDEFPKADSTTEALGALRPAFDKAG
TVTAGNASGINDGAAALVIMEESAALAAGLTPLARIKSYASGGVPPALMGMGPVPATQKALQLAGLQLADIDLIEANEAF
AAQFLAVGKTLGFDSEKVNVNGGAIALGHPIGASGARILVTLLHAMQARDKTLGLATLCIGGGQGIAMVIERLN

Specific function: SHORT-CHAIN FATTY ACIDS METABOLISM. [C]

COG id: COG0183

COG function: function code I; Acetyl-CoA acetyltransferase

Gene ontology:

Cell location: Cytoplasm (Potential) [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the thiolase family [H]

Homologues:

Organism=Homo sapiens, GI148539872, Length=390, Percent_Identity=53.8461538461538, Blast_Score=401, Evalue=1e-112,
Organism=Homo sapiens, GI167614485, Length=396, Percent_Identity=43.1818181818182, Blast_Score=309, Evalue=4e-84,
Organism=Homo sapiens, GI4557237, Length=396, Percent_Identity=44.4444444444444, Blast_Score=301, Evalue=7e-82,
Organism=Homo sapiens, GI4501853, Length=401, Percent_Identity=41.1471321695761, Blast_Score=254, Evalue=1e-67,
Organism=Homo sapiens, GI4504327, Length=425, Percent_Identity=32.4705882352941, Blast_Score=171, Evalue=8e-43,
Organism=Homo sapiens, GI194353979, Length=396, Percent_Identity=32.3232323232323, Blast_Score=152, Evalue=3e-37,
Organism=Escherichia coli, GI1788554, Length=394, Percent_Identity=98.7309644670051, Blast_Score=780, Evalue=0.0,
Organism=Escherichia coli, GI87082165, Length=392, Percent_Identity=57.6530612244898, Blast_Score=449, Evalue=1e-127,
Organism=Escherichia coli, GI1787663, Length=404, Percent_Identity=46.039603960396, Blast_Score=313, Evalue=1e-86,
Organism=Escherichia coli, GI48994986, Length=405, Percent_Identity=42.962962962963, Blast_Score=277, Evalue=9e-76,
Organism=Escherichia coli, GI1788683, Length=423, Percent_Identity=35.6973995271868, Blast_Score=202, Evalue=3e-53,
Organism=Caenorhabditis elegans, GI133906874, Length=393, Percent_Identity=45.29262086514, Blast_Score=320, Evalue=7e-88,
Organism=Caenorhabditis elegans, GI25147385, Length=393, Percent_Identity=44.0203562340967, Blast_Score=300, Evalue=1e-81,
Organism=Caenorhabditis elegans, GI17535921, Length=394, Percent_Identity=42.6395939086294, Blast_Score=276, Evalue=2e-74,
Organism=Caenorhabditis elegans, GI17535917, Length=404, Percent_Identity=34.1584158415842, Blast_Score=190, Evalue=1e-48,
Organism=Caenorhabditis elegans, GI17551802, Length=426, Percent_Identity=32.3943661971831, Blast_Score=184, Evalue=6e-47,
Organism=Saccharomyces cerevisiae, GI6325229, Length=398, Percent_Identity=44.7236180904523, Blast_Score=301, Evalue=2e-82,
Organism=Saccharomyces cerevisiae, GI6322031, Length=397, Percent_Identity=37.7833753148615, Blast_Score=218, Evalue=1e-57,
Organism=Drosophila melanogaster, GI24655093, Length=394, Percent_Identity=52.5380710659898, Blast_Score=402, Evalue=1e-112,
Organism=Drosophila melanogaster, GI24640423, Length=393, Percent_Identity=46.5648854961832, Blast_Score=308, Evalue=5e-84,
Organism=Drosophila melanogaster, GI17648125, Length=392, Percent_Identity=43.3673469387755, Blast_Score=288, Evalue=5e-78,
Organism=Drosophila melanogaster, GI17137578, Length=425, Percent_Identity=32.2352941176471, Blast_Score=171, Evalue=1e-42,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR002155
- InterPro:   IPR016039
- InterPro:   IPR016038
- InterPro:   IPR020615
- InterPro:   IPR020610
- InterPro:   IPR020617
- InterPro:   IPR020613
- InterPro:   IPR020616 [H]

Pfam domain/function: PF02803 Thiolase_C; PF00108 Thiolase_N [H]

EC number: =2.3.1.9 [H]

Molecular weight: Translated: 40370; Mature: 40370

Theoretical pI: Translated: 6.78; Mature: 6.78

Prosite motif: PS00098 THIOLASE_1 ; PS00737 THIOLASE_2 ; PS00099 THIOLASE_3

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.3 %Cys     (Translated Protein)
3.0 %Met     (Translated Protein)
4.3 %Cys+Met (Translated Protein)
1.3 %Cys     (Mature Protein)
3.0 %Met     (Mature Protein)
4.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKNCVIVSAVRTAIGSFNGSLASTSAIDLGATVIKAAIERAKIDSLHIDEVIMGNVLQAG
CCCCEEHHHHHHHHHCCCCCCCCCCHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHC
LGQNPARQALLKSGLAETVCGFTVNKVCGSGLKSVALAAQAIQAGQAQSIVAGGMENMSL
CCCCHHHHHHHHHCHHHHHHHHHHHHHHCCCHHHHHHHHHHHHCCCHHHHHHCCCCCCCC
APYLLDAKARSGYRLGDGQVYDVILRDGLMCATHGYHMGITAENVAKEYGITREMQDELA
CCEEEECCCCCCCCCCCCCEEEEHHHCCEEEEECCEEECCCHHHHHHHHCCCHHHHHHHH
LHSQRKAAAAIESGAFTAEIVPVNVVTRKKTFVFSQDEFPKADSTTEALGALRPAFDKAG
HHHHHHHHHHHHCCCEEEEEEEEEEEECCEEEEECCCCCCCCCCHHHHHHHHHHHHCCCC
TVTAGNASGINDGAAALVIMEESAALAAGLTPLARIKSYASGGVPPALMGMGPVPATQKA
CEECCCCCCCCCCCEEEEEEECCCHHHHCCCHHHHHHHHHCCCCCHHHHCCCCCCHHHHH
LQLAGLQLADIDLIEANEAFAAQFLAVGKTLGFDSEKVNVNGGAIALGHPIGASGARILV
HHHHCCEEECEEEEECCHHHHHHHHHHHHHCCCCCCEEECCCCEEEECCCCCCCHHHHHH
TLLHAMQARDKTLGLATLCIGGGQGIAMVIERLN
HHHHHHHHCCCCCCEEEEEEECCCCHHHHHHHCC
>Mature Secondary Structure
MKNCVIVSAVRTAIGSFNGSLASTSAIDLGATVIKAAIERAKIDSLHIDEVIMGNVLQAG
CCCCEEHHHHHHHHHCCCCCCCCCCHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHC
LGQNPARQALLKSGLAETVCGFTVNKVCGSGLKSVALAAQAIQAGQAQSIVAGGMENMSL
CCCCHHHHHHHHHCHHHHHHHHHHHHHHCCCHHHHHHHHHHHHCCCHHHHHHCCCCCCCC
APYLLDAKARSGYRLGDGQVYDVILRDGLMCATHGYHMGITAENVAKEYGITREMQDELA
CCEEEECCCCCCCCCCCCCEEEEHHHCCEEEEECCEEECCCHHHHHHHHCCCHHHHHHHH
LHSQRKAAAAIESGAFTAEIVPVNVVTRKKTFVFSQDEFPKADSTTEALGALRPAFDKAG
HHHHHHHHHHHHCCCEEEEEEEEEEEECCEEEEECCCCCCCCCCHHHHHHHHHHHHCCCC
TVTAGNASGINDGAAALVIMEESAALAAGLTPLARIKSYASGGVPPALMGMGPVPATQKA
CEECCCCCCCCCCCEEEEEEECCCHHHHCCCHHHHHHHHHCCCCCHHHHCCCCCCHHHHH
LQLAGLQLADIDLIEANEAFAAQFLAVGKTLGFDSEKVNVNGGAIALGHPIGASGARILV
HHHHCCEEECEEEEECCHHHHHHHHHHHHHCCCCCCEEECCCCEEEECCCCCCCHHHHHH
TLLHAMQARDKTLGLATLCIGGGQGIAMVIERLN
HHHHHHHHCCCCCCEEEEEEECCCCHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 9097040; 9278503 [H]