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The map label for this gene is yejM

Identifier: 218700660

GI number: 218700660

Start: 2398532

End: 2400292

Strand: Direct

Name: yejM

Synonym: ECIAI39_2329

Alternate gene names: 218700660

Gene position: 2398532-2400292 (Clockwise)

Preceding gene: 218700659

Following gene: 218700662

Centisome position: 46.74

GC content: 51.79

Gene sequence:

>1761_bases
ATGGTAACTCATCGTCAGCGCTACCGTGAAAAAGTCTCCCAGATGGTCAGTTGGGGGCACTGGTTTGCACTGTTCAATAT
TCTGCTTTCGCTCGTCATTGGCAGCCGTTACCTGTTTATCGCCGACTGGCCGACAACGCTTGCTGGTCGCATTTATTCCT
ACGTAAGCATTATCGGTCATTTCAGCTTCCTGGTGTTCGCCACCTACTTACTGATCCTCTTCCCGCTGACCTTTATCGTC
GGCTCCCAGAGGCTGATGAGGTTTTTGTCCGTCATTCTGGCAACGGCGGGAATGACGCTATTACTGATCGATAGCGAAGT
CTTTACTCGTTTCCATCTCCATCTTAATCCCATCGTCTGGCAACTGGTTATCAACCCAGACGAAAATGAGATGGCGCGCG
ACTGGCAGCTGATGTTCATCAGCGTGCCGGTTATTTTATTGCTTGAACTGGTGTTTGCGACGTGGAGCTGGCAAAAGCTA
CGCAGCCTGACGCGTCGTCGGCGCTTCGCGCGCCCGCTGGCCGCATTCTTATTTATCGCCTTTATCGCCTCGCATGTGGT
TTATATCTGGGCCGATGCCAACTTCTATCGCCCGATCACCATGCAACGCGCTAACCTGCCGCTTTCGTACCCGATGACGG
CGCGACGTTTTCTTGAGAAGCATGGTCTGCTTGATGCGCAGGAGTATCAACGCCGTCTCATTGAGCAAGGTAATCCAGAC
GCTGTTTCCGTTCAGTATCCGTTAAGCGAACTGCGCTATCGCGATATGGGCACCGGGCAGAATGTGCTGTTGATTACTGT
CGATGGCCTGAACTACTCACGCTTCGAGAAGCAGATGCCTGCGCTGGCAGGTTTTGCTGAGCAAAATATTTCGTTCACGC
GCCATATGAGCTCCGGCAACACTACAGACAACGGCATCTTTGGCCTGTTCTATGGCATCTCGCCGAGCTATATGGATGGC
ATTCTGTCGACCCGTACGCCTGCGGCATTAATTACTGCGCTTAATCAGCAAGGCTATCAGCTGGGGTTATTCTCATCAGA
TGGCTTTACCAGCCCGCTGTATCGCCAGGCATTGTTGTCAGATTTCTCGATGCCGAGCGTACGTACCCAATCCGACGAGC
AGACCGCCACGCAGTGGATCAACTGGCTGGGCCGCTACGCACAAGAAGATAACCGCTGGTTCTCGTGGGTTTCTTTCAAT
GGCACTAACATTGACGACAGCAATCAGCAGGCATTTGCACGGAAATATAGCCGGGCGGCAGGCAATGTCGATGACCAGAT
CAACCGCGTGCTCAATGCACTGCGTGATTCTGGCAAACTGGACAATACGGTGGTGATTATCACTGCCGGTCGGGGTATTC
CGCTGAGCGAAGAGGAAGAAACCTTTGACTGGTCCCACGGTCATCTGCAGGTGCCATTAGTGATTCACTGGCCAGGCACG
CCGGCGCAGCGTATTAATGCTCTGACTGATCATACCGATCTGATGACGACGCTGATGCAACGCCTGCTACATGTCAGCAC
ACCTGCCAGCGAATATTCGCAAGGTCAGGATTTGTTCAACCCTCAACGCCGTCATTACTGGGTTACTGCAGCGGATAACG
ATACGCTGGCAATTACCACCCCGAAAAAGACGCTGGTGCTGAACAATAACGGTAAATACCGCACTTACAACTTACGTGGT
GAAAGAGTGAAAGATGAAAAACCACAGTTAAGTTTGTTATTGCAAGTACTGACAGATGAGAAGCGTTTTATCGCTAACTG
A

Upstream 100 bases:

>100_bases
GCCCCGGCCCAACGCCAGGCAATTGCCAACTCTTTTGCCCGCGCCTTACAGTCCTCTATCAACGAAGACAAAGCGCACTA
AGGGAAACAGATAACAGGTT

Downstream 100 bases:

>100_bases
TTAATTATAAATCAGTTAGCGAAATATCTTACTTGCAATCGGTGTGGAAAACGGTAGTATTAGCAGCCACGAGTCGGCAC
GTAGCGCAGCCTGGTAGCGC

Product: putative hydrolase, inner membrane-associated

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 586; Mature: 586

Protein sequence:

>586_residues
MVTHRQRYREKVSQMVSWGHWFALFNILLSLVIGSRYLFIADWPTTLAGRIYSYVSIIGHFSFLVFATYLLILFPLTFIV
GSQRLMRFLSVILATAGMTLLLIDSEVFTRFHLHLNPIVWQLVINPDENEMARDWQLMFISVPVILLLELVFATWSWQKL
RSLTRRRRFARPLAAFLFIAFIASHVVYIWADANFYRPITMQRANLPLSYPMTARRFLEKHGLLDAQEYQRRLIEQGNPD
AVSVQYPLSELRYRDMGTGQNVLLITVDGLNYSRFEKQMPALAGFAEQNISFTRHMSSGNTTDNGIFGLFYGISPSYMDG
ILSTRTPAALITALNQQGYQLGLFSSDGFTSPLYRQALLSDFSMPSVRTQSDEQTATQWINWLGRYAQEDNRWFSWVSFN
GTNIDDSNQQAFARKYSRAAGNVDDQINRVLNALRDSGKLDNTVVIITAGRGIPLSEEEETFDWSHGHLQVPLVIHWPGT
PAQRINALTDHTDLMTTLMQRLLHVSTPASEYSQGQDLFNPQRRHYWVTAADNDTLAITTPKKTLVLNNNGKYRTYNLRG
ERVKDEKPQLSLLLQVLTDEKRFIAN

Sequences:

>Translated_586_residues
MVTHRQRYREKVSQMVSWGHWFALFNILLSLVIGSRYLFIADWPTTLAGRIYSYVSIIGHFSFLVFATYLLILFPLTFIV
GSQRLMRFLSVILATAGMTLLLIDSEVFTRFHLHLNPIVWQLVINPDENEMARDWQLMFISVPVILLLELVFATWSWQKL
RSLTRRRRFARPLAAFLFIAFIASHVVYIWADANFYRPITMQRANLPLSYPMTARRFLEKHGLLDAQEYQRRLIEQGNPD
AVSVQYPLSELRYRDMGTGQNVLLITVDGLNYSRFEKQMPALAGFAEQNISFTRHMSSGNTTDNGIFGLFYGISPSYMDG
ILSTRTPAALITALNQQGYQLGLFSSDGFTSPLYRQALLSDFSMPSVRTQSDEQTATQWINWLGRYAQEDNRWFSWVSFN
GTNIDDSNQQAFARKYSRAAGNVDDQINRVLNALRDSGKLDNTVVIITAGRGIPLSEEEETFDWSHGHLQVPLVIHWPGT
PAQRINALTDHTDLMTTLMQRLLHVSTPASEYSQGQDLFNPQRRHYWVTAADNDTLAITTPKKTLVLNNNGKYRTYNLRG
ERVKDEKPQLSLLLQVLTDEKRFIAN
>Mature_586_residues
MVTHRQRYREKVSQMVSWGHWFALFNILLSLVIGSRYLFIADWPTTLAGRIYSYVSIIGHFSFLVFATYLLILFPLTFIV
GSQRLMRFLSVILATAGMTLLLIDSEVFTRFHLHLNPIVWQLVINPDENEMARDWQLMFISVPVILLLELVFATWSWQKL
RSLTRRRRFARPLAAFLFIAFIASHVVYIWADANFYRPITMQRANLPLSYPMTARRFLEKHGLLDAQEYQRRLIEQGNPD
AVSVQYPLSELRYRDMGTGQNVLLITVDGLNYSRFEKQMPALAGFAEQNISFTRHMSSGNTTDNGIFGLFYGISPSYMDG
ILSTRTPAALITALNQQGYQLGLFSSDGFTSPLYRQALLSDFSMPSVRTQSDEQTATQWINWLGRYAQEDNRWFSWVSFN
GTNIDDSNQQAFARKYSRAAGNVDDQINRVLNALRDSGKLDNTVVIITAGRGIPLSEEEETFDWSHGHLQVPLVIHWPGT
PAQRINALTDHTDLMTTLMQRLLHVSTPASEYSQGQDLFNPQRRHYWVTAADNDTLAITTPKKTLVLNNNGKYRTYNLRG
ERVKDEKPQLSLLLQVLTDEKRFIAN

Specific function: Unknown

COG id: COG3083

COG function: function code R; Predicted hydrolase of alkaline phosphatase superfamily

Gene ontology:

Cell location: Cell inner membrane; Multi-pass membrane protein

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: To H.influenzae HI_0842

Homologues:

Organism=Escherichia coli, GI1788515, Length=586, Percent_Identity=100, Blast_Score=1203, Evalue=0.0,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): YEJM_ECO57 (P0AD29)

Other databases:

- EMBL:   AE005174
- EMBL:   BA000007
- PIR:   B85858
- PIR:   H91013
- RefSeq:   NP_288771.1
- RefSeq:   NP_311107.1
- ProteinModelPortal:   P0AD29
- EnsemblBacteria:   EBESCT00000026466
- EnsemblBacteria:   EBESCT00000058700
- GeneID:   916785
- GeneID:   957420
- GenomeReviews:   AE005174_GR
- GenomeReviews:   BA000007_GR
- KEGG:   ece:Z3447
- KEGG:   ecs:ECs3080
- GeneTree:   EBGT00050000011744
- HOGENOM:   HBG296982
- OMA:   SLVGHFS
- ProtClustDB:   CLSK880324
- BioCyc:   ECOL83334:ECS3080-MONOMER
- InterPro:   IPR017849
- InterPro:   IPR017850
- InterPro:   IPR012159
- InterPro:   IPR000917
- Gene3D:   G3DSA:3.40.720.10
- PIRSF:   PIRSF004950

Pfam domain/function: PF00884 Sulfatase; SSF53649 Alkaline_phosphatase_core

EC number: NA

Molecular weight: Translated: 67297; Mature: 67297

Theoretical pI: Translated: 9.40; Mature: 9.40

Prosite motif: PS00430 TONB_DEPENDENT_REC_1

Important sites: NA

Signals:

None

Transmembrane regions:

HASH(0x2352cf74)-; HASH(0x2054e834)-; HASH(0x236564cc)-; HASH(0x22f3a890)-; HASH(0x226aa308)-;

Cys/Met content:

0.0 %Cys     (Translated Protein)
2.6 %Met     (Translated Protein)
2.6 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
2.6 %Met     (Mature Protein)
2.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MVTHRQRYREKVSQMVSWGHWFALFNILLSLVIGSRYLFIADWPTTLAGRIYSYVSIIGH
CCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCEEEEECCCHHHHHHHHHHHHHHHH
FSFLVFATYLLILFPLTFIVGSQRLMRFLSVILATAGMTLLLIDSEVFTRFHLHLNPIVW
HHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHCCCEEEEECHHHHHEEEEEECCEEE
QLVINPDENEMARDWQLMFISVPVILLLELVFATWSWQKLRSLTRRRRFARPLAAFLFIA
EEEECCCCCCHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHH
FIASHVVYIWADANFYRPITMQRANLPLSYPMTARRFLEKHGLLDAQEYQRRLIEQGNPD
HHHHCEEEEEECCCEECCEEEEECCCCCCCCHHHHHHHHHCCCCCHHHHHHHHHHCCCCC
AVSVQYPLSELRYRDMGTGQNVLLITVDGLNYSRFEKQMPALAGFAEQNISFTRHMSSGN
EEEEECCHHHHHHHCCCCCCEEEEEEECCCCHHHHHHHHHHHHHHHHCCCEEEEECCCCC
TTDNGIFGLFYGISPSYMDGILSTRTPAALITALNQQGYQLGLFSSDGFTSPLYRQALLS
CCCCCEEEEEECCCHHHHHHHHHCCCHHHHHHHHCCCCCEEEEECCCCCCCHHHHHHHHH
DFSMPSVRTQSDEQTATQWINWLGRYAQEDNRWFSWVSFNGTNIDDSNQQAFARKYSRAA
CCCCCCCCCCCCHHHHHHHHHHHHHHHCCCCCEEEEEEECCCCCCCCCHHHHHHHHHHHC
GNVDDQINRVLNALRDSGKLDNTVVIITAGRGIPLSEEEETFDWSHGHLQVPLVIHWPGT
CCHHHHHHHHHHHHHCCCCCCCEEEEEECCCCCCCCCCCCCCCCCCCEEEEEEEEECCCC
PAQRINALTDHTDLMTTLMQRLLHVSTPASEYSQGQDLFNPQRRHYWVTAADNDTLAITT
CHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHCCCCCCCCCCCEEEEEECCCCEEEEEC
PKKTLVLNNNGKYRTYNLRGERVKDEKPQLSLLLQVLTDEKRFIAN
CCEEEEECCCCCEEEEECCCCCCCCCCHHHHHHHHHHHCCHHHCCH
>Mature Secondary Structure
MVTHRQRYREKVSQMVSWGHWFALFNILLSLVIGSRYLFIADWPTTLAGRIYSYVSIIGH
CCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCEEEEECCCHHHHHHHHHHHHHHHH
FSFLVFATYLLILFPLTFIVGSQRLMRFLSVILATAGMTLLLIDSEVFTRFHLHLNPIVW
HHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHCCCEEEEECHHHHHEEEEEECCEEE
QLVINPDENEMARDWQLMFISVPVILLLELVFATWSWQKLRSLTRRRRFARPLAAFLFIA
EEEECCCCCCHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHH
FIASHVVYIWADANFYRPITMQRANLPLSYPMTARRFLEKHGLLDAQEYQRRLIEQGNPD
HHHHCEEEEEECCCEECCEEEEECCCCCCCCHHHHHHHHHCCCCCHHHHHHHHHHCCCCC
AVSVQYPLSELRYRDMGTGQNVLLITVDGLNYSRFEKQMPALAGFAEQNISFTRHMSSGN
EEEEECCHHHHHHHCCCCCCEEEEEEECCCCHHHHHHHHHHHHHHHHCCCEEEEECCCCC
TTDNGIFGLFYGISPSYMDGILSTRTPAALITALNQQGYQLGLFSSDGFTSPLYRQALLS
CCCCCEEEEEECCCHHHHHHHHHCCCHHHHHHHHCCCCCEEEEECCCCCCCHHHHHHHHH
DFSMPSVRTQSDEQTATQWINWLGRYAQEDNRWFSWVSFNGTNIDDSNQQAFARKYSRAA
CCCCCCCCCCCCHHHHHHHHHHHHHHHCCCCCEEEEEEECCCCCCCCCHHHHHHHHHHHC
GNVDDQINRVLNALRDSGKLDNTVVIITAGRGIPLSEEEETFDWSHGHLQVPLVIHWPGT
CCHHHHHHHHHHHHHCCCCCCCEEEEEECCCCCCCCCCCCCCCCCCCEEEEEEEEECCCC
PAQRINALTDHTDLMTTLMQRLLHVSTPASEYSQGQDLFNPQRRHYWVTAADNDTLAITT
CHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHCCCCCCCCCCCEEEEEECCCCEEEEEC
PKKTLVLNNNGKYRTYNLRGERVKDEKPQLSLLLQVLTDEKRFIAN
CCEEEEECCCCCEEEEECCCCCCCCCCHHHHHHHHHHHCCHHHCCH

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 11206551; 11258796