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The map label for this gene is yeiL

Identifier: 218700634

GI number: 218700634

Start: 2369512

End: 2370171

Strand: Direct

Name: yeiL

Synonym: ECIAI39_2303

Alternate gene names: 218700634

Gene position: 2369512-2370171 (Clockwise)

Preceding gene: 218700631

Following gene: 218700641

Centisome position: 46.17

GC content: 43.79

Gene sequence:

>660_bases
ATGAGTGAATCCGCGTTTAAGGATTGCTTTTCAACGGATGTTTCAGCCGATACTCGGCTGTTTCATTTTTTAGCGCGTGA
CTACATAGTGCAGGAAGGGCAACAGCCATCCTGGCTGTTTTACCTGACGCGAGGCCGCGCCAGGCTTTACGCCACGCTAG
CTAATGGTCGCGTGTCGCTGATCGATTTCTTTGCCGCCCCCTGTTTTATTGGTGAGATTGAGTTAATCGATAAAGACCAT
GAACCGCGTGCGGTGCAGGCTATTGAAGAGTGTTGGTGCCTTGCGCTCCCTATGAAACATTACCGCCAGCTGTTATTAAA
CGACACGCTATTTTTACGAAAACTTTGCGTTACTTTAAGCCATAAAAATTATCGCAATATTGTTTCTTTAACTCAGAATC
AATCATTTCCGTTACTGAATCGCCTGGCAGCATTTATATTACTCTCGCAGGAGGGTGATCTTTATCACGAAAAACATACC
CAGGCGGCAGAGTATTTAAGTGTTACTTATCGTCACTTGCTATATGTGATCGCTCAACTCATTCACGACGGTTTATTAAT
TAAAAGCAAGAAAGGATATTTAATTAAAAACAGAAAGCAGTTATCAGGACTGGCGCTGGAGATGGACCCGGAAAATAAAT
TCTCCGGGATGATGCAGTAA

Upstream 100 bases:

>100_bases
TTGAATCTTAACAACAGCGTATGTATGCTAAATATGAGAAATCTCATAGCGGATAAACATCGTGAAAGAAATCCACAATA
ATGATCTTAAGCAGCAATTG

Downstream 100 bases:

>100_bases
AAAATAATCTGCAATAGCGCGATTGCCGGATGCAACGCTTAATAAGGCGTACATCCGGCAATGAAACTCAAGCTAAACCA
ATAAAGAACCCGGCAATGGT

Product: DNA-binding transcriptional activator YeiL

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 219; Mature: 218

Protein sequence:

>219_residues
MSESAFKDCFSTDVSADTRLFHFLARDYIVQEGQQPSWLFYLTRGRARLYATLANGRVSLIDFFAAPCFIGEIELIDKDH
EPRAVQAIEECWCLALPMKHYRQLLLNDTLFLRKLCVTLSHKNYRNIVSLTQNQSFPLLNRLAAFILLSQEGDLYHEKHT
QAAEYLSVTYRHLLYVIAQLIHDGLLIKSKKGYLIKNRKQLSGLALEMDPENKFSGMMQ

Sequences:

>Translated_219_residues
MSESAFKDCFSTDVSADTRLFHFLARDYIVQEGQQPSWLFYLTRGRARLYATLANGRVSLIDFFAAPCFIGEIELIDKDH
EPRAVQAIEECWCLALPMKHYRQLLLNDTLFLRKLCVTLSHKNYRNIVSLTQNQSFPLLNRLAAFILLSQEGDLYHEKHT
QAAEYLSVTYRHLLYVIAQLIHDGLLIKSKKGYLIKNRKQLSGLALEMDPENKFSGMMQ
>Mature_218_residues
SESAFKDCFSTDVSADTRLFHFLARDYIVQEGQQPSWLFYLTRGRARLYATLANGRVSLIDFFAAPCFIGEIELIDKDHE
PRAVQAIEECWCLALPMKHYRQLLLNDTLFLRKLCVTLSHKNYRNIVSLTQNQSFPLLNRLAAFILLSQEGDLYHEKHTQ
AAEYLSVTYRHLLYVIAQLIHDGLLIKSKKGYLIKNRKQLSGLALEMDPENKFSGMMQ

Specific function: Transcription regulator involved in mid-term, stationary-phase viability under nitrogen starvation. Might control expression of the salvage pathways or in some other way repress the recycling of nucleobases to nucleic acids and enhance their use as genera

COG id: COG0664

COG function: function code T; cAMP-binding proteins - catabolite gene activator and regulatory subunit of cAMP-dependent protein kinases

Gene ontology:

Cell location: Cytoplasm (Probable) [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 HTH crp-type DNA-binding domain [H]

Homologues:

Organism=Escherichia coli, GI1788487, Length=219, Percent_Identity=96.8036529680365, Blast_Score=444, Evalue=1e-126,

Paralogues:

None

Copy number: 10-20 Molecules/Cell [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR018490
- InterPro:   IPR000595
- InterPro:   IPR012318
- InterPro:   IPR014710 [H]

Pfam domain/function: PF00027 cNMP_binding [H]

EC number: NA

Molecular weight: Translated: 25324; Mature: 25192

Theoretical pI: Translated: 8.21; Mature: 8.21

Prosite motif: PS50042 CNMP_BINDING_3 ; PS51063 HTH_CRP_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.3 %Cys     (Translated Protein)
2.3 %Met     (Translated Protein)
4.6 %Cys+Met (Translated Protein)
2.3 %Cys     (Mature Protein)
1.8 %Met     (Mature Protein)
4.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSESAFKDCFSTDVSADTRLFHFLARDYIVQEGQQPSWLFYLTRGRARLYATLANGRVSL
CCCHHHHHHHHCCCCCHHHHHHHHHHHHHHHCCCCCCEEEEEECCCEEEEEEECCCCCHH
IDFFAAPCFIGEIELIDKDHEPRAVQAIEECWCLALPMKHYRQLLLNDTLFLRKLCVTLS
HHHHHHHHHCCEEEEECCCCCCHHHHHHHHHHHHHCCHHHHHHHHHCHHHHHHHHHHHHC
HKNYRNIVSLTQNQSFPLLNRLAAFILLSQEGDLYHEKHTQAAEYLSVTYRHLLYVIAQL
CCCHHHHHHHHCCCCCHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHH
IHDGLLIKSKKGYLIKNRKQLSGLALEMDPENKFSGMMQ
HHCCEEEECCCCCEEECCHHHCCEEEEECCCCCCCCCCC
>Mature Secondary Structure 
SESAFKDCFSTDVSADTRLFHFLARDYIVQEGQQPSWLFYLTRGRARLYATLANGRVSL
CCHHHHHHHHCCCCCHHHHHHHHHHHHHHHCCCCCCEEEEEECCCEEEEEEECCCCCHH
IDFFAAPCFIGEIELIDKDHEPRAVQAIEECWCLALPMKHYRQLLLNDTLFLRKLCVTLS
HHHHHHHHHCCEEEEECCCCCCHHHHHHHHHHHHHCCHHHHHHHHHCHHHHHHHHHHHHC
HKNYRNIVSLTQNQSFPLLNRLAAFILLSQEGDLYHEKHTQAAEYLSVTYRHLLYVIAQL
CCCHHHHHHHHCCCCCHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHH
IHDGLLIKSKKGYLIKNRKQLSGLALEMDPENKFSGMMQ
HHCCEEEECCCCCEEECCHHHCCEEEEECCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 11206551; 11258796 [H]