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The map label for this gene is focA

Identifier: 218700578

GI number: 218700578

Start: 2301157

End: 2302014

Strand: Direct

Name: focA

Synonym: ECIAI39_2244

Alternate gene names: 218700578

Gene position: 2301157-2302014 (Clockwise)

Preceding gene: 218700577

Following gene: 218700579

Centisome position: 44.84

GC content: 48.37

Gene sequence:

>858_bases
GTGAAAGCTGACAACCCTTTTGATCTTTTACTTCCTGCAGCAATGGCCAAAGTGGCCGAAGAGGCAGGTGTCTATAAAGC
AACGAAACATCCGCTTAAGACTTTCTATCTGGCGATTACCGCCGGTGTTTTCATCTCAATCGCATTCGTCTTCTATATCA
CAGCAACCACTGGCACAGGAACAATGCCCTTCGGCATGGCAAAACTGGTTGGCGGCATTTGCTTCTCTCTGGGGCTGATT
CTTTGTGTTGTCTGCGGAGCCGATCTCTTTACTTCCACAGTGTTGATTGTTGTTGCTAAGGCGAGTGGGCGCATCACCTG
GGGTCAGTTGGCGAAAAACTGGCTAAATGTCTATTTTGGCAACCTGGTCGGCGCACTGCTGTTTGTACTTTTAATGTGGC
TTTCCGGCGAGTATATGACCGCGAATGGTCAATGGGGACTAAACGTCCTACAAACCGCCGACCACAAAGTGCACCATACT
TTTATTGAGGCCGTCTGCCTTGGTATCCTGGCAAACCTAATGGTATGTCTGGCAGTATGGATGAGTTATTCTGGCCGCAG
CCTGATGGATAAAGCGTTCATTATGGTGCTGCCGGTCGCGATGTTTGTTGCCAGCGGTTTTGAGCACAGTATCGCAAACA
TGTTTATGATCCCGATGGGTATTGTAATCCGCGACTTCGCATCTCCGGAATTCTGGACCGCTGTCGGTTCTGCACCGGAA
AATTTTTCTCACCTGACCGTGATGAATTTCATCACTGATAACCTGATTCCGGTTACGATCGGTAATATTATCGGCGGTGG
TTTGTTGGTTGGGTTGACATACTGGGTCATTTACCTGCGTGAAAACGATCACCATTAA

Upstream 100 bases:

>100_bases
TTACGGGCCTATAAGCCAGGCGAGATATGATCTATATCAATTTCTCATCTATAATGCTTTGTTAGTATCTCGTCGCCGAC
TTAATAAAGAGAGAGTTAGT

Downstream 100 bases:

>100_bases
TGGTTGTCGAAGTACGCAGTAAATAAAAAATCCACTTAAGAAGGTAGGTGTTACATGTCCGAGCTTAATGAAAAGTTAGC
CACAGCCTGGGAAGGTTTTA

Product: formate transporter

Products: formate [Periplasm]; formate [Cytoplasm] [C]

Alternate protein names: Formate channel 1

Number of amino acids: Translated: 285; Mature: 285

Protein sequence:

>285_residues
MKADNPFDLLLPAAMAKVAEEAGVYKATKHPLKTFYLAITAGVFISIAFVFYITATTGTGTMPFGMAKLVGGICFSLGLI
LCVVCGADLFTSTVLIVVAKASGRITWGQLAKNWLNVYFGNLVGALLFVLLMWLSGEYMTANGQWGLNVLQTADHKVHHT
FIEAVCLGILANLMVCLAVWMSYSGRSLMDKAFIMVLPVAMFVASGFEHSIANMFMIPMGIVIRDFASPEFWTAVGSAPE
NFSHLTVMNFITDNLIPVTIGNIIGGGLLVGLTYWVIYLRENDHH

Sequences:

>Translated_285_residues
MKADNPFDLLLPAAMAKVAEEAGVYKATKHPLKTFYLAITAGVFISIAFVFYITATTGTGTMPFGMAKLVGGICFSLGLI
LCVVCGADLFTSTVLIVVAKASGRITWGQLAKNWLNVYFGNLVGALLFVLLMWLSGEYMTANGQWGLNVLQTADHKVHHT
FIEAVCLGILANLMVCLAVWMSYSGRSLMDKAFIMVLPVAMFVASGFEHSIANMFMIPMGIVIRDFASPEFWTAVGSAPE
NFSHLTVMNFITDNLIPVTIGNIIGGGLLVGLTYWVIYLRENDHH
>Mature_285_residues
MKADNPFDLLLPAAMAKVAEEAGVYKATKHPLKTFYLAITAGVFISIAFVFYITATTGTGTMPFGMAKLVGGICFSLGLI
LCVVCGADLFTSTVLIVVAKASGRITWGQLAKNWLNVYFGNLVGALLFVLLMWLSGEYMTANGQWGLNVLQTADHKVHHT
FIEAVCLGILANLMVCLAVWMSYSGRSLMDKAFIMVLPVAMFVASGFEHSIANMFMIPMGIVIRDFASPEFWTAVGSAPE
NFSHLTVMNFITDNLIPVTIGNIIGGGLLVGLTYWVIYLRENDHH

Specific function: Involved in the bidirectional transport of formate

COG id: COG2116

COG function: function code P; Formate/nitrite family of transporters

Gene ontology:

Cell location: Cell inner membrane; Multi-pass membrane protein

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the FNT transporter (TC 2.A.44) family

Homologues:

Organism=Escherichia coli, GI1787132, Length=285, Percent_Identity=100, Blast_Score=578, Evalue=1e-166,
Organism=Escherichia coli, GI1788837, Length=282, Percent_Identity=50.354609929078, Blast_Score=281, Evalue=3e-77,
Organism=Escherichia coli, GI87082249, Length=268, Percent_Identity=28.7313432835821, Blast_Score=75, Evalue=5e-15,
Organism=Saccharomyces cerevisiae, GI6321779, Length=207, Percent_Identity=29.4685990338164, Blast_Score=81, Evalue=2e-16,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): FOCA_ECO57 (P0AC25)

Other databases:

- EMBL:   AE005174
- EMBL:   BA000007
- PIR:   A85616
- PIR:   C90752
- RefSeq:   NP_286779.1
- RefSeq:   NP_309014.1
- PDB:   3KCU
- PDB:   3KCV
- PDBsum:   3KCU
- PDBsum:   3KCV
- ProteinModelPortal:   P0AC25
- SMR:   P0AC25
- EnsemblBacteria:   EBESCT00000025545
- EnsemblBacteria:   EBESCT00000059613
- GeneID:   917772
- GeneID:   958866
- GenomeReviews:   AE005174_GR
- GenomeReviews:   BA000007_GR
- KEGG:   ece:Z1250
- KEGG:   ecs:ECs0987
- GeneTree:   EBGT00050000009717
- HOGENOM:   HBG726755
- OMA:   ANGGWGL
- ProtClustDB:   PRK10805
- BioCyc:   ECOL83334:ECS0987-MONOMER
- InterPro:   IPR000292
- TIGRFAMs:   TIGR00790

Pfam domain/function: PF01226 Form_Nir_trans

EC number: NA

Molecular weight: Translated: 30991; Mature: 30991

Theoretical pI: Translated: 6.94; Mature: 6.94

Prosite motif: PS01005 FORMATE_NITRITE_TP_1; PS01006 FORMATE_NITRITE_TP_2

Important sites: NA

Signals:

None

Transmembrane regions:

HASH(0x2352cf8c)-; HASH(0x22e2872c)-; HASH(0x2341b4a8)-; HASH(0x2341b538)-; HASH(0x22d0af5c)-; HASH(0x233a235c)-;

Cys/Met content:

1.8 %Cys     (Translated Protein)
5.3 %Met     (Translated Protein)
7.0 %Cys+Met (Translated Protein)
1.8 %Cys     (Mature Protein)
5.3 %Met     (Mature Protein)
7.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKADNPFDLLLPAAMAKVAEEAGVYKATKHPLKTFYLAITAGVFISIAFVFYITATTGTG
CCCCCCHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCC
TMPFGMAKLVGGICFSLGLILCVVCGADLFTSTVLIVVAKASGRITWGQLAKNWLNVYFG
CCCHHHHHHHHHHHHHHHHHHHHHHCHHHHHHHHHHEEECCCCCCCHHHHHHHHHHHHHH
NLVGALLFVLLMWLSGEYMTANGQWGLNVLQTADHKVHHTFIEAVCLGILANLMVCLAVW
HHHHHHHHHHHHHHCCCEEECCCCCCCHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHH
MSYSGRSLMDKAFIMVLPVAMFVASGFEHSIANMFMIPMGIVIRDFASPEFWTAVGSAPE
HCCCCCHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHCCCCHHHHHHCCCCC
NFSHLTVMNFITDNLIPVTIGNIIGGGLLVGLTYWVIYLRENDHH
CCCHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHEEEECCCCH
>Mature Secondary Structure
MKADNPFDLLLPAAMAKVAEEAGVYKATKHPLKTFYLAITAGVFISIAFVFYITATTGTG
CCCCCCHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCC
TMPFGMAKLVGGICFSLGLILCVVCGADLFTSTVLIVVAKASGRITWGQLAKNWLNVYFG
CCCHHHHHHHHHHHHHHHHHHHHHHCHHHHHHHHHHEEECCCCCCCHHHHHHHHHHHHHH
NLVGALLFVLLMWLSGEYMTANGQWGLNVLQTADHKVHHTFIEAVCLGILANLMVCLAVW
HHHHHHHHHHHHHHCCCEEECCCCCCCHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHH
MSYSGRSLMDKAFIMVLPVAMFVASGFEHSIANMFMIPMGIVIRDFASPEFWTAVGSAPE
HCCCCCHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHCCCCHHHHHHCCCCC
NFSHLTVMNFITDNLIPVTIGNIIGGGLLVGLTYWVIYLRENDHH
CCCHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHEEEECCCCH

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: formate [Cytoplasm]; formate [Periplasm] [C]

Specific reaction: formate [Cytoplasm] = formate [Periplasm] formate [Periplasm] = formate [Cytoplasm] [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 7.0

TargetDB status: NA

Availability: NA

References: 11206551; 11258796