Click here to switch to the map view.

The map label for this gene is ompF

Identifier: 218700552

GI number: 218700552

Start: 2267390

End: 2268463

Strand: Direct

Name: ompF

Synonym: ECIAI39_2218

Alternate gene names: 218700552

Gene position: 2267390-2268463 (Clockwise)

Preceding gene: 218700551

Following gene: 218700553

Centisome position: 44.18

GC content: 49.35

Gene sequence:

>1074_bases
ATGATGAAGCGCAATATTCTGGCAGTGATCGTCCCTGCTCTGTTAGTAGCAGGTACTGCAAACGCTGCAGAAATCTATAA
CAAAGATGGCAACAAAGTAGATCTGTACGGTAAAGCTGTCGGTCTGCATTATTTTTCTAAAGACAATGGTGTAAACAGTT
ACGGCGGAAACGGCGACAAAACTTATGCCCGTCTTGGTTTTAAAGGGGAAACACAAATCAATTCCGATCTGACCGGCTAC
GGTCAGTGGGAATATAACTTCCAGGGTAACAACTCTGAAGGCGCTGATGCCCAGAAAGGTAACAAAACTCGTCTGGCATT
CGCAGGTCTGAAATTCGCTGATGCGGGTTCTATCGACTACGGTCGTAACTACGGTGTGGTTTACGACGCACTGGGTTACA
CCGATATGCTGCCTGAGTTTGGCGGTGACACGGCATACAGCGATGACTTCTTCGTTGGTCGTGTAGGCGGCGTTGCTACC
TATCGTAACTCCAACTTCTTCGGTCTGGTTGACGGCCTGAACTTTGCCGTTCAGTACCTGGGCAAAAACGAACGTGCTGG
TATTCCTGAGCGTTCCAACGGCGACGGCGTCGGTGGCTCTATCAGCTACGAATTCGAAGGCTTTGGTATCGTAGGTGCTT
ACGGTGCAGCAGACCGTACCGACGCCCAGGAAGCCGAATTCCGCGGTCAGGGTAAAAAAGCTGAACAGTGGGCAACTGGT
CTGAAGTATGACGCGAACAACATCTACCTGGCAGCGAACTACGGTGAAACCCGTAACGCTACGCCAATTGAAGGCGGCTT
CGCCAACAAAACGCAAGACGTTCTGTTAGTTGCGCAATACCAGTTCGATTTCGGTCTGCGTCCGTCCATCGCTTACACCA
AATCTAAAGCGAAAGACGTAGAAGGTATCGGTGATGTTGATCTGGTGAACTACTTTGAAGTGGGCGCAACCTACTACTTC
AACAAAAACATGTCCACCTATGTTGACTACATCATCAACCAGATCGATTCTGACAACAAACTGGGCGTAGGTTCTGACGA
CACCGTTGCTGTGGGTATCGTTTACCAGTTCTAA

Upstream 100 bases:

>100_bases
AAGACACCAAACTCTCATCAATAGTTCCGTAAATTTTTATTGACAGAACTTATTGACGGCAGTGGCAGGTGTCATAAAAA
AACCATGAGGGTAATAAATA

Downstream 100 bases:

>100_bases
TAGCACACCTCTTTGTTAAATGCCGAAAAAACAGGACGTTGGTCCTGTTTTTTTTATGCCTTCCAGAGCAATCTCACGTC
TTGCAAAAACAGCCTGCGTT

Product: outer membrane protein F

Products: NA

Alternate protein names: Outer membrane protein 1A; Outer membrane protein B; Outer membrane protein IA; Porin OmpF [H]

Number of amino acids: Translated: 357; Mature: 357

Protein sequence:

>357_residues
MMKRNILAVIVPALLVAGTANAAEIYNKDGNKVDLYGKAVGLHYFSKDNGVNSYGGNGDKTYARLGFKGETQINSDLTGY
GQWEYNFQGNNSEGADAQKGNKTRLAFAGLKFADAGSIDYGRNYGVVYDALGYTDMLPEFGGDTAYSDDFFVGRVGGVAT
YRNSNFFGLVDGLNFAVQYLGKNERAGIPERSNGDGVGGSISYEFEGFGIVGAYGAADRTDAQEAEFRGQGKKAEQWATG
LKYDANNIYLAANYGETRNATPIEGGFANKTQDVLLVAQYQFDFGLRPSIAYTKSKAKDVEGIGDVDLVNYFEVGATYYF
NKNMSTYVDYIINQIDSDNKLGVGSDDTVAVGIVYQF

Sequences:

>Translated_357_residues
MMKRNILAVIVPALLVAGTANAAEIYNKDGNKVDLYGKAVGLHYFSKDNGVNSYGGNGDKTYARLGFKGETQINSDLTGY
GQWEYNFQGNNSEGADAQKGNKTRLAFAGLKFADAGSIDYGRNYGVVYDALGYTDMLPEFGGDTAYSDDFFVGRVGGVAT
YRNSNFFGLVDGLNFAVQYLGKNERAGIPERSNGDGVGGSISYEFEGFGIVGAYGAADRTDAQEAEFRGQGKKAEQWATG
LKYDANNIYLAANYGETRNATPIEGGFANKTQDVLLVAQYQFDFGLRPSIAYTKSKAKDVEGIGDVDLVNYFEVGATYYF
NKNMSTYVDYIINQIDSDNKLGVGSDDTVAVGIVYQF
>Mature_357_residues
MMKRNILAVIVPALLVAGTANAAEIYNKDGNKVDLYGKAVGLHYFSKDNGVNSYGGNGDKTYARLGFKGETQINSDLTGY
GQWEYNFQGNNSEGADAQKGNKTRLAFAGLKFADAGSIDYGRNYGVVYDALGYTDMLPEFGGDTAYSDDFFVGRVGGVAT
YRNSNFFGLVDGLNFAVQYLGKNERAGIPERSNGDGVGGSISYEFEGFGIVGAYGAADRTDAQEAEFRGQGKKAEQWATG
LKYDANNIYLAANYGETRNATPIEGGFANKTQDVLLVAQYQFDFGLRPSIAYTKSKAKDVEGIGDVDLVNYFEVGATYYF
NKNMSTYVDYIINQIDSDNKLGVGSDDTVAVGIVYQF

Specific function: OmpF is a porin that forms passive diffusion pores which allow small molecular weight hydrophilic materials across the outer membrane. It is also a receptor for the bacteriophage T2 [H]

COG id: COG3203

COG function: function code M; Outer membrane protein (porin)

Gene ontology:

Cell location: Cell outer membrane; Multi-pass membrane protein [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the Gram-negative porin family [H]

Homologues:

Organism=Escherichia coli, GI1787160, Length=363, Percent_Identity=92.5619834710744, Blast_Score=652, Evalue=0.0,
Organism=Escherichia coli, GI1786436, Length=359, Percent_Identity=61.0027855153203, Blast_Score=431, Evalue=1e-122,
Organism=Escherichia coli, GI1788544, Length=376, Percent_Identity=60.1063829787234, Blast_Score=406, Evalue=1e-114,
Organism=Escherichia coli, GI1787641, Length=362, Percent_Identity=57.182320441989, Blast_Score=371, Evalue=1e-104,

Paralogues:

None

Copy number: 1980 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 2160 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 17600 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 12744 Molecules/Cell In: Growth Phase,

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001897
- InterPro:   IPR013793
- InterPro:   IPR001702 [H]

Pfam domain/function: PF00267 Porin_1 [H]

EC number: NA

Molecular weight: Translated: 38675; Mature: 38675

Theoretical pI: Translated: 4.46; Mature: 4.46

Prosite motif: PS00576 GRAM_NEG_PORIN

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
1.1 %Met     (Translated Protein)
1.1 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
1.1 %Met     (Mature Protein)
1.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MMKRNILAVIVPALLVAGTANAAEIYNKDGNKVDLYGKAVGLHYFSKDNGVNSYGGNGDK
CCCCHHHHHHHHHHHHCCCCCHHHHHCCCCCEEEEEEEEEEEEEEECCCCCCCCCCCCCC
TYARLGFKGETQINSDLTGYGQWEYNFQGNNSEGADAQKGNKTRLAFAGLKFADAGSIDY
EEEEECCCCCCCCCCCCCCCEEEEEEECCCCCCCCCCCCCCCEEEEEECCEEECCCCCCC
GRNYGVVYDALGYTDMLPEFGGDTAYSDDFFVGRVGGVATYRNSNFFGLVDGLNFAVQYL
CCCCCEEEECCCCHHHHHHCCCCCCCCCCEEEECCCCEEEEECCCEEEEEHHHHHHHHHH
GKNERAGIPERSNGDGVGGSISYEFEGFGIVGAYGAADRTDAQEAEFRGQGKKAEQWATG
CCCCCCCCCCCCCCCCCCCEEEEEECCEEEEEECCCCCCCCCHHHHHCCCCCCHHHHHCC
LKYDANNIYLAANYGETRNATPIEGGFANKTQDVLLVAQYQFDFGLRPSIAYTKSKAKDV
CEECCCEEEEEECCCCCCCCCCCCCCCCCCCCEEEEEEEEEECCCCCCCEEECCCCCCCC
EGIGDVDLVNYFEVGATYYFNKNMSTYVDYIINQIDSDNKLGVGSDDTVAVGIVYQF
CCCCCCHHHHHEECCEEEEECCCHHHHHHHHHHHCCCCCCCCCCCCCCEEEEEEEEC
>Mature Secondary Structure
MMKRNILAVIVPALLVAGTANAAEIYNKDGNKVDLYGKAVGLHYFSKDNGVNSYGGNGDK
CCCCHHHHHHHHHHHHCCCCCHHHHHCCCCCEEEEEEEEEEEEEEECCCCCCCCCCCCCC
TYARLGFKGETQINSDLTGYGQWEYNFQGNNSEGADAQKGNKTRLAFAGLKFADAGSIDY
EEEEECCCCCCCCCCCCCCCEEEEEEECCCCCCCCCCCCCCCEEEEEECCEEECCCCCCC
GRNYGVVYDALGYTDMLPEFGGDTAYSDDFFVGRVGGVATYRNSNFFGLVDGLNFAVQYL
CCCCCEEEECCCCHHHHHHCCCCCCCCCCEEEECCCCEEEEECCCEEEEEHHHHHHHHHH
GKNERAGIPERSNGDGVGGSISYEFEGFGIVGAYGAADRTDAQEAEFRGQGKKAEQWATG
CCCCCCCCCCCCCCCCCCCEEEEEECCEEEEEECCCCCCCCCHHHHHCCCCCCHHHHHCC
LKYDANNIYLAANYGETRNATPIEGGFANKTQDVLLVAQYQFDFGLRPSIAYTKSKAKDV
CEECCCEEEEEECCCCCCCCCCCCCCCCCCCCEEEEEEEEEECCCCCCCEEECCCCCCCC
EGIGDVDLVNYFEVGATYYFNKNMSTYVDYIINQIDSDNKLGVGSDDTVAVGIVYQF
CCCCCCHHHHHEECCEEEEECCCHHHHHHHHHHHCCCCCCCCCCCCCCEEEEEEEEC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 7.0

TargetDB status: NA

Availability: NA

References: 6294623; 8905232; 9278503; 7037455; 7049161; 2997131; 9298646; 9629924; 1380671; 7524100; 9843370 [H]