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The map label for this gene is rutE

Identifier: 218700481

GI number: 218700481

Start: 2192775

End: 2193365

Strand: Direct

Name: rutE

Synonym: ECIAI39_2147

Alternate gene names: 218700481

Gene position: 2192775-2193365 (Clockwise)

Preceding gene: 218700480

Following gene: 218700482

Centisome position: 42.73

GC content: 57.19

Gene sequence:

>591_bases
ATGAACGAAGCCGTTAGCCCTGGTGCGCTTAGCACCCTGTTCACCGATGCCCGCACTCACAACGGCTGGCGGGAGACCCC
CGTCAGCGATGAGACATTACGGGAGATTTATGCCCTGATGAAATGGGGGCCGACATCAGCTAACTGTTCTCCGGCACGGA
TCGTGTTTATCCGCACTGCAGAAGGAAAAGAACGTCTGCGCCCGGCGCTTTCCAGCGGCAATCTGCAAAAAACCCTAACC
GCGCCCGTCACCGCTATCGTCGCCTGGGACAGTGAATTTTATGAACGGTTACCACAGCTGTTTCCTCACGGCGATGCCCG
CAGTTGGTTTACCTCCAGCCCACAACTTGCCGAAGAAACAGCGTTTCGCAACAGTTCCATGCAGGCGGCCTATCTGATCG
TCGCCTGCCGGGCGCTGGGGCTGGATACCGGCCCGATGTCCGGCTTTGACCGTCAACACGTGGACGACGCCTTTTTTACG
GGCAGCACGCTGAAGAGCAATCTGCTGATTAATATCGGCTATGGCGATAGCAGCAAGCTTTATGCGCGCCTGCCACGTCT
GTCCTTTGAAGAAGCCTGCGGGCTGTTGTAA

Upstream 100 bases:

>100_bases
CGCCTGCAACGTGACCGATCCCGAAACGTTTAATGCTCTGTTACTCAACGGGCTTGCCAGCCTGTTACATCACCGTGAAG
CCGCCCTGTAAGGAATTGCT

Downstream 100 bases:

>100_bases
GGAGCAAACTATGAACATAGTTGATCAACAAACCTTTCGCGATGCGATGTCCTGTATGGGCGCGGCGGTCAATATCATCA
CCACAGACGGTCCAGCCGGG

Product: hypothetical protein

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 196; Mature: 196

Protein sequence:

>196_residues
MNEAVSPGALSTLFTDARTHNGWRETPVSDETLREIYALMKWGPTSANCSPARIVFIRTAEGKERLRPALSSGNLQKTLT
APVTAIVAWDSEFYERLPQLFPHGDARSWFTSSPQLAEETAFRNSSMQAAYLIVACRALGLDTGPMSGFDRQHVDDAFFT
GSTLKSNLLINIGYGDSSKLYARLPRLSFEEACGLL

Sequences:

>Translated_196_residues
MNEAVSPGALSTLFTDARTHNGWRETPVSDETLREIYALMKWGPTSANCSPARIVFIRTAEGKERLRPALSSGNLQKTLT
APVTAIVAWDSEFYERLPQLFPHGDARSWFTSSPQLAEETAFRNSSMQAAYLIVACRALGLDTGPMSGFDRQHVDDAFFT
GSTLKSNLLINIGYGDSSKLYARLPRLSFEEACGLL
>Mature_196_residues
MNEAVSPGALSTLFTDARTHNGWRETPVSDETLREIYALMKWGPTSANCSPARIVFIRTAEGKERLRPALSSGNLQKTLT
APVTAIVAWDSEFYERLPQLFPHGDARSWFTSSPQLAEETAFRNSSMQAAYLIVACRALGLDTGPMSGFDRQHVDDAFFT
GSTLKSNLLINIGYGDSSKLYARLPRLSFEEACGLL

Specific function: May reduce toxic product malonic semialdehyde to 3- hydroxypropionic acid, which is excreted [H]

COG id: COG0778

COG function: function code C; Nitroreductase

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the nitroreductase family. HadB/RutE subfamily [H]

Homologues:

Organism=Escherichia coli, GI1787243, Length=196, Percent_Identity=98.9795918367347, Blast_Score=400, Evalue=1e-113,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000415 [H]

Pfam domain/function: PF00881 Nitroreductase [H]

EC number: =1.1.1.298 [H]

Molecular weight: Translated: 21598; Mature: 21598

Theoretical pI: Translated: 6.26; Mature: 6.26

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.5 %Cys     (Translated Protein)
2.0 %Met     (Translated Protein)
3.6 %Cys+Met (Translated Protein)
1.5 %Cys     (Mature Protein)
2.0 %Met     (Mature Protein)
3.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNEAVSPGALSTLFTDARTHNGWRETPVSDETLREIYALMKWGPTSANCSPARIVFIRTA
CCCCCCCCHHHHHHHHHHHCCCCCCCCCCHHHHHHHHHHHHCCCCCCCCCCEEEEEEEEC
EGKERLRPALSSGNLQKTLTAPVTAIVAWDSEFYERLPQLFPHGDARSWFTSSPQLAEET
CCHHHHHHHHCCCCCCEEHHCCEEEEEEECHHHHHHHHHHCCCCCCCCCCCCCCHHHHHH
AFRNSSMQAAYLIVACRALGLDTGPMSGFDRQHVDDAFFTGSTLKSNLLINIGYGDSSKL
HHCCCCCHHHHHHHHHHHHCCCCCCCCCCCHHHCCHHHHCCCCCCCCEEEEECCCCCCHH
YARLPRLSFEEACGLL
HHHCCCCCHHHHCCCC
>Mature Secondary Structure
MNEAVSPGALSTLFTDARTHNGWRETPVSDETLREIYALMKWGPTSANCSPARIVFIRTA
CCCCCCCCHHHHHHHHHHHCCCCCCCCCCHHHHHHHHHHHHCCCCCCCCCCEEEEEEEEC
EGKERLRPALSSGNLQKTLTAPVTAIVAWDSEFYERLPQLFPHGDARSWFTSSPQLAEET
CCHHHHHHHHCCCCCCEEHHCCEEEEEEECHHHHHHHHHHCCCCCCCCCCCCCCHHHHHH
AFRNSSMQAAYLIVACRALGLDTGPMSGFDRQHVDDAFFTGSTLKSNLLINIGYGDSSKL
HHCCCCCHHHHHHHHHHHHCCCCCCCCCCCHHHCCHHHHCCCCCCCCEEEEECCCCCCHH
YARLPRLSFEEACGLL
HHHCCCCCHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA