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The map label for this gene is lsrG

Identifier: 218700138

GI number: 218700138

Start: 1873317

End: 1873607

Strand: Direct

Name: lsrG

Synonym: ECIAI39_1781

Alternate gene names: 218700138

Gene position: 1873317-1873607 (Clockwise)

Preceding gene: 218700137

Following gene: 218700139

Centisome position: 36.5

GC content: 46.39

Gene sequence:

>291_bases
ATGCACGTCACACTGGTTGAAATTAACGTTCATGAAGACAAGGTTGACGAGTTTATCGAAGTTTTTCGCCAGAACCACCT
GGGCTCTGTACAGGAAGAAGGCAATTTGCGCTTCGATGTCTTACAGGACCCGGAAGTGAATTCGCGCTTTTTTATCTACG
AAGCCTATAAAGATGAAGACGCAGTGGCGTTCCATAAAACCACGCCCCACTACAAAACCTGTGTCGCAAAACTGGAAGCT
TTAATGACCGGGCCGCGTAAAAAACGTCTGTTCAATGGATTGATGCCGTGA

Upstream 100 bases:

>100_bases
AAGCCGTACAGGCGGTGGTACACCATAACGAAACGGCTGATCGGGCATATGAACTCTATCTGAGTGAAAAACAGTAACTG
CGGATTTAAGGAGAAGAATT

Downstream 100 bases:

>100_bases
GGCGAATTTATCAGTTTTATCTACACTAGGGGTAACGCGCTGACGGGAGTAAAAATATGTCTGACTGGAACCCCTCTTTA
TATCTGCACTTTGCCGCTGA

Product: autoinducer-2 (AI-2) modifying protein LsrG

Products: NA

Alternate protein names: AI-2-degrading protein lsrG [H]

Number of amino acids: Translated: 96; Mature: 96

Protein sequence:

>96_residues
MHVTLVEINVHEDKVDEFIEVFRQNHLGSVQEEGNLRFDVLQDPEVNSRFFIYEAYKDEDAVAFHKTTPHYKTCVAKLEA
LMTGPRKKRLFNGLMP

Sequences:

>Translated_96_residues
MHVTLVEINVHEDKVDEFIEVFRQNHLGSVQEEGNLRFDVLQDPEVNSRFFIYEAYKDEDAVAFHKTTPHYKTCVAKLEA
LMTGPRKKRLFNGLMP
>Mature_96_residues
MHVTLVEINVHEDKVDEFIEVFRQNHLGSVQEEGNLRFDVLQDPEVNSRFFIYEAYKDEDAVAFHKTTPHYKTCVAKLEA
LMTGPRKKRLFNGLMP

Specific function: Involved in the degradation of phospho-AI-2, thereby terminating induction of the lsr operon and closing the AI-2 signaling cycle. Catalyzes the cleavage of phosphorylated 4,5- dihydroxy-2,3-pentanedione (P-DPD) to 2-phosphoglycolic acid (PG) and another

COG id: COG1359

COG function: function code S; Uncharacterized conserved protein

Gene ontology:

Cell location: Cytoplasm (Potential) [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the lsrG family [H]

Homologues:

Organism=Escherichia coli, GI1787797, Length=96, Percent_Identity=97.9166666666667, Blast_Score=199, Evalue=3e-53,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR007138
- InterPro:   IPR011008 [H]

Pfam domain/function: PF03992 ABM [H]

EC number: NA

Molecular weight: Translated: 11223; Mature: 11223

Theoretical pI: Translated: 5.98; Mature: 5.98

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.0 %Cys     (Translated Protein)
3.1 %Met     (Translated Protein)
4.2 %Cys+Met (Translated Protein)
1.0 %Cys     (Mature Protein)
3.1 %Met     (Mature Protein)
4.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MHVTLVEINVHEDKVDEFIEVFRQNHLGSVQEEGNLRFDVLQDPEVNSRFFIYEAYKDED
CEEEEEEEECCHHHHHHHHHHHHHCCCCCCCCCCCEEEEEECCCCCCCEEEEEEECCCCC
AVAFHKTTPHYKTCVAKLEALMTGPRKKRLFNGLMP
CEEEECCCCHHHHHHHHHHHHHCCCHHHHHHCCCCC
>Mature Secondary Structure
MHVTLVEINVHEDKVDEFIEVFRQNHLGSVQEEGNLRFDVLQDPEVNSRFFIYEAYKDED
CEEEEEEEECCHHHHHHHHHHHHHCCCCCCCCCCCEEEEEECCCCCCCEEEEEEECCCCC
AVAFHKTTPHYKTCVAKLEALMTGPRKKRLFNGLMP
CEEEECCCCHHHHHHHHHHHHHCCCHHHHHHCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 11206551; 11258796 [H]