Click here to switch to the map view.

The map label for this gene is puuB

Identifier: 218700023

GI number: 218700023

Start: 1725836

End: 1727116

Strand: Direct

Name: puuB

Synonym: ECIAI39_1652

Alternate gene names: 218700023

Gene position: 1725836-1727116 (Clockwise)

Preceding gene: 218700022

Following gene: 218700024

Centisome position: 33.63

GC content: 59.33

Gene sequence:

>1281_bases
ATGACCGAACACACCAGCAGTTACTACGCCGCCAGTGCGAATAAATACGCGCCATTCGACACGCTGAATGAGTCGATTTC
CTGCGACGTTTGCGTGGTCGGCGGCGGCTATACCGGGCTTTCCTCCGCGCTGCATCTGGCGGAAGCGGGCTTTGACGTGG
TGGTTCTCGAAGCCTCACGCATCGGCTTTGGCGCGAGCGGACGCAACGGCGGGCAGCTTGTGAACTCCTACAGCCGCGAC
ATCGACGTGATCGAAAAAAGCTACGGCATGGATACCGCCCGTATGCTCGGCAGCATGATGTTCGAGGGTGGCGAGATCAT
CCGCGAACGTATCAAACGTTATCAGATTGACTGCGACTATCGCCCCGGCGGTCTGTTTGTGGCGATGAATGATAAACAGC
TCGCCACACTCGAAGAGCAGAAAGAGAACTGGGAACGCTACGGCAATAAACAGCTGGAGTTGCTGGACGCCAACGCTATT
CGCCGCGAGGTCGCCAGCGATCGCTACACTGGTGCGCTGCTGGATCACAGCGGTGGGCATATTCACCCGCTAAACCTCGC
CATTGGTGAAGCCGATGCCATCCGCCTGAACGGCGGGCGTGTGTATGAGCTTTCCGCCGTGACGCAGATCCAGCACACCA
CGCCAGCCGTGGTGCGGACTGCCAATGGTCAGGTGACGGCGAAGTATGTGATTGTCGCCGGGAATGCCTACCTGGGCGAT
AAAGTAGAGCCGGAACTAGCGAAACGCAGCATGCCGTGCGGCACGCAGGTGATCACCACCGAACGGCTGTCGGAAGATTT
AGCCCGTTCGCTGATCCCGAAAAACTACTGTGTGGAAGACTGTAACTATCTGCTTGATTACTACCGTCTTACCGCCGATA
ACCGCCTGCTGTACGGCGGCGGCGTGGTCTACGGCGCGCGCGACCCGGACGATGTTGAGCGCCTTGTGGTGCCGAAACTG
CTGAAAACCTTCCCGCAACTGAAGGGCGTGAAAATTGATTACCGCTGGACGGGCAACTTCCTGCTGACCCTGTCACGCAT
GCCGCAGTTTGGTCGCCTCGATACCAACATCTACTACATGCAGGGCTACAGCGGCCACGGCGTCACCTGTACCCACCTCG
CCGGGCGTTTGATTGCTGAACTGCTGCGCGGTGACGCCGAGCGTTTCGACGCCTTCGCCAATCTGCCGCATTACCCGTTC
CCCGGCGGGCGCACGCTGCGCGTTCCGTTTACCGCGATGGGCGCGGCTTATTACAGCCTGCGCGATCGTCTGGGCGTTTA
A

Upstream 100 bases:

>100_bases
GGCGGCTATAAGCAGAGCGGCAACGGGCGCGACAAATCCCTGCATGCCCTTGAAAAATTCACCGAACTGAAAACCATCTG
GATAAGCCTGGAGGCCTGAA

Downstream 100 bases:

>100_bases
TTTCCGATTAACAGTGAAGAGTCAAAAGGTGTGAAACATGAGCAACAATGAATTCCATCAGCGTCGTCTTTCTGCTACCC
CACGCGGGGTTGGCGTGATG

Product: gamma-Glu-putrescine oxidase, FAD/NAD(P)-binding

Products: NA

Alternate protein names: Gamma-Glu-Put oxidase; Gamma-glutamylputrescine oxidase [H]

Number of amino acids: Translated: 426; Mature: 425

Protein sequence:

>426_residues
MTEHTSSYYAASANKYAPFDTLNESISCDVCVVGGGYTGLSSALHLAEAGFDVVVLEASRIGFGASGRNGGQLVNSYSRD
IDVIEKSYGMDTARMLGSMMFEGGEIIRERIKRYQIDCDYRPGGLFVAMNDKQLATLEEQKENWERYGNKQLELLDANAI
RREVASDRYTGALLDHSGGHIHPLNLAIGEADAIRLNGGRVYELSAVTQIQHTTPAVVRTANGQVTAKYVIVAGNAYLGD
KVEPELAKRSMPCGTQVITTERLSEDLARSLIPKNYCVEDCNYLLDYYRLTADNRLLYGGGVVYGARDPDDVERLVVPKL
LKTFPQLKGVKIDYRWTGNFLLTLSRMPQFGRLDTNIYYMQGYSGHGVTCTHLAGRLIAELLRGDAERFDAFANLPHYPF
PGGRTLRVPFTAMGAAYYSLRDRLGV

Sequences:

>Translated_426_residues
MTEHTSSYYAASANKYAPFDTLNESISCDVCVVGGGYTGLSSALHLAEAGFDVVVLEASRIGFGASGRNGGQLVNSYSRD
IDVIEKSYGMDTARMLGSMMFEGGEIIRERIKRYQIDCDYRPGGLFVAMNDKQLATLEEQKENWERYGNKQLELLDANAI
RREVASDRYTGALLDHSGGHIHPLNLAIGEADAIRLNGGRVYELSAVTQIQHTTPAVVRTANGQVTAKYVIVAGNAYLGD
KVEPELAKRSMPCGTQVITTERLSEDLARSLIPKNYCVEDCNYLLDYYRLTADNRLLYGGGVVYGARDPDDVERLVVPKL
LKTFPQLKGVKIDYRWTGNFLLTLSRMPQFGRLDTNIYYMQGYSGHGVTCTHLAGRLIAELLRGDAERFDAFANLPHYPF
PGGRTLRVPFTAMGAAYYSLRDRLGV
>Mature_425_residues
TEHTSSYYAASANKYAPFDTLNESISCDVCVVGGGYTGLSSALHLAEAGFDVVVLEASRIGFGASGRNGGQLVNSYSRDI
DVIEKSYGMDTARMLGSMMFEGGEIIRERIKRYQIDCDYRPGGLFVAMNDKQLATLEEQKENWERYGNKQLELLDANAIR
REVASDRYTGALLDHSGGHIHPLNLAIGEADAIRLNGGRVYELSAVTQIQHTTPAVVRTANGQVTAKYVIVAGNAYLGDK
VEPELAKRSMPCGTQVITTERLSEDLARSLIPKNYCVEDCNYLLDYYRLTADNRLLYGGGVVYGARDPDDVERLVVPKLL
KTFPQLKGVKIDYRWTGNFLLTLSRMPQFGRLDTNIYYMQGYSGHGVTCTHLAGRLIAELLRGDAERFDAFANLPHYPFP
GGRTLRVPFTAMGAAYYSLRDRLGV

Specific function: Involved in the breakdown of putrescine via the oxidation of L-glutamylputrescine [H]

COG id: COG0665

COG function: function code E; Glycine/D-amino acid oxidases (deaminating)

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: NA

Homologues:

Organism=Escherichia coli, GI1787559, Length=426, Percent_Identity=99.5305164319249, Blast_Score=875, Evalue=0.0,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR006076 [H]

Pfam domain/function: PF01266 DAO [H]

EC number: 1.-.-.- [C]

Molecular weight: Translated: 47142; Mature: 47011

Theoretical pI: Translated: 6.60; Mature: 6.60

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.6 %Cys     (Translated Protein)
2.3 %Met     (Translated Protein)
4.0 %Cys+Met (Translated Protein)
1.6 %Cys     (Mature Protein)
2.1 %Met     (Mature Protein)
3.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTEHTSSYYAASANKYAPFDTLNESISCDVCVVGGGYTGLSSALHLAEAGFDVVVLEASR
CCCCCCCEEEECCCCCCCHHHCCCCCCCCEEEECCCCHHHHHHHHHHHCCCCEEEEEECC
IGFGASGRNGGQLVNSYSRDIDVIEKSYGMDTARMLGSMMFEGGEIIRERIKRYQIDCDY
CCCCCCCCCCHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHCCHHHHHHHHHHEEECCCC
RPGGLFVAMNDKQLATLEEQKENWERYGNKQLELLDANAIRREVASDRYTGALLDHSGGH
CCCEEEEEECCCHHHHHHHHHHHHHHHCCCEEEEECHHHHHHHHHCCCCCEEEEECCCCE
IHPLNLAIGEADAIRLNGGRVYELSAVTQIQHTTPAVVRTANGQVTAKYVIVAGNAYLGD
EEEEEEEECCCCEEEECCCEEEEEEHHHHHCCCCCEEEEECCCCEEEEEEEEECCCCCCC
KVEPELAKRSMPCGTQVITTERLSEDLARSLIPKNYCVEDCNYLLDYYRLTADNRLLYGG
CCCHHHHHCCCCCCCEEEEHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHCCCCCEEEEEC
GVVYGARDPDDVERLVVPKLLKTFPQLKGVKIDYRWTGNFLLTLSRMPQFGRLDTNIYYM
EEEECCCCHHHHHHHHHHHHHHHHHHHCCCEEEEEECCCEEEEEECCCCCCCCCCEEEEE
QGYSGHGVTCTHLAGRLIAELLRGDAERFDAFANLPHYPFPGGRTLRVPFTAMGAAYYSL
ECCCCCCEEHHHHHHHHHHHHHHCCHHHHHHHHCCCCCCCCCCCEEEECHHHHHHHHHHH
RDRLGV
HHHHCC
>Mature Secondary Structure 
TEHTSSYYAASANKYAPFDTLNESISCDVCVVGGGYTGLSSALHLAEAGFDVVVLEASR
CCCCCCEEEECCCCCCCHHHCCCCCCCCEEEECCCCHHHHHHHHHHHCCCCEEEEEECC
IGFGASGRNGGQLVNSYSRDIDVIEKSYGMDTARMLGSMMFEGGEIIRERIKRYQIDCDY
CCCCCCCCCCHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHCCHHHHHHHHHHEEECCCC
RPGGLFVAMNDKQLATLEEQKENWERYGNKQLELLDANAIRREVASDRYTGALLDHSGGH
CCCEEEEEECCCHHHHHHHHHHHHHHHCCCEEEEECHHHHHHHHHCCCCCEEEEECCCCE
IHPLNLAIGEADAIRLNGGRVYELSAVTQIQHTTPAVVRTANGQVTAKYVIVAGNAYLGD
EEEEEEEECCCCEEEECCCEEEEEEHHHHHCCCCCEEEEECCCCEEEEEEEEECCCCCCC
KVEPELAKRSMPCGTQVITTERLSEDLARSLIPKNYCVEDCNYLLDYYRLTADNRLLYGG
CCCHHHHHCCCCCCCEEEEHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHCCCCCEEEEEC
GVVYGARDPDDVERLVVPKLLKTFPQLKGVKIDYRWTGNFLLTLSRMPQFGRLDTNIYYM
EEEECCCCHHHHHHHHHHHHHHHHHHHCCCEEEEEECCCEEEEEECCCCCCCCCCEEEEE
QGYSGHGVTCTHLAGRLIAELLRGDAERFDAFANLPHYPFPGGRTLRVPFTAMGAAYYSL
ECCCCCCEEHHHHHHHHHHHHHHCCHHHHHHHHCCCCCCCCCCCEEEECHHHHHHHHHHH
RDRLGV
HHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 9097039; 9278503; 1840553; 7984428 [H]