| Definition | Escherichia coli IAI39 chromosome, complete genome. |
|---|---|
| Accession | NC_011750 |
| Length | 5,132,068 |
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The map label for this gene is topA
Identifier: 218699984
GI number: 218699984
Start: 1681967
End: 1684564
Strand: Direct
Name: topA
Synonym: ECIAI39_1613
Alternate gene names: 218699984
Gene position: 1681967-1684564 (Clockwise)
Preceding gene: 218699982
Following gene: 218699985
Centisome position: 32.77
GC content: 51.73
Gene sequence:
>2598_bases ATGGGTAAAGCTCTTGTCATCGTTGAGTCCCCGGCAAAAGCCAAAACGATCAACAAGTATCTGGGTAGTGACTACGTGGT GAAATCCAGCGTCGGTCACATCCGCGATTTGCCGACCAGTGGCTCAGCTGCCAAAAAGAGTGCCGACTCTACCTCCACCA AGACGGCTAAAAAGCCTAAAAAGGATGAACGTGGCGCTCTCGTCAACCGTATGGGGGTTGACCCGTGGCACAATTGGGAG GCGCACTATGAAGTGTTGCCTGGTAAAGAGAAGGTCGTCTCTGAACTGAAACAACTGGCTGAAAAAGCCGACCACATCTA TCTCGCAACCGACCTTGACCGCGAAGGGGAAGCCATTGCATGGCACCTGCGGGAAGTGATTGGTGGTGATGATGCGCGCT ATAGCCGAGTGGTGTTTAACGAAATTACTAAAAACGCGATCCGCCAGGCATTTAACAAACCAGGTGAGCTGAATATTGAT CGTGTTAATGCCCAGCAGGCGCGTCGCTTTATGGACCGCGTGGTGGGGTATATGGTTTCGCCGCTGCTGTGGAAAAAGAT CGCTCGTGGTCTGTCTGCCGGTCGTGTGCAGTCGGTGGCGGTTCGCCTGGTGGTCGAGCGTGAGCGTGAAATTAAAGCGT TCGTGCCGGAAGAGTTCTGGGAAGTCGATGCCAGCACGACCACGCCATCTGGTGAAGCGTTGGCGTTACAGGTCACTCAT CAGAACGACAAACCGTTCCGCCCGGTCAACAGAGAACAAACTCAGGCTGCGGTAAGTCTGCTGGAAAAAGCGCGCTACAG CGTGCTGGAACGTGAAGACAAACCGACAACCAGTAAACCTGGCGCTCCTTTTATTACCTCTACGCTGCAACAAGCTGCCA GCACCCGTCTTGGATTTGGCGTGAAAAAAACCATGATGATGGCGCAGCGTTTGTATGAAGCAGGCTATATCACTTACATG CGTACCGACTCCACTAACCTGAGTCAGGACGCGGTAAATATGGTTCGCGGTTATATCAGCGATAATTTTGGTAAGAAATA TCTGCCGGAAAGTCCGAATCAGTACGCCAGCAAAGAAAACTCACAGGAAGCGCACGAAGCGATTCGTCCTTCTGACGTCA ATGTGATGGCGGAATCGCTGAAGGATATGGAAGCAGATGCGCAGAAACTGTACCAGTTAATCTGGCGTCAGTTCGTTGCC TGCCAGATGACCCCAGCGAAATATGACTCCACGACGCTGACCGTTGGTGCGGGCGATTTCCGCCTGAAAGCACGCGGTCG TATTTTGCGCTTTGATGGCTGGACAAAAGTGATGCCTGCACTGCGTAAAGGCGATGAAGATCGTATCTTACCGGCAGTCG ATAAAGGCGATGCTCTGACGCTCGTTGAACTGACACCAGCCCAGCACTTTACCAAGCCGCCAGCCCGTTTTAGTGAAGCA TCGCTGGTTAAAGAGCTGGAAAAACGCGGTATCGGTCGTCCGTCTACCTATGCATCGATCATTTCGACCATTCAGGATCG TGGCTATGTGCGAGTAGAAAATCGTCGTTTCTATGCGGAAAAAATGGGCGAAATCGTCACCGATCGCCTTGAAGAAAATT TCCGTGAGTTAATGAACTACGACTTCACCGCGCAGATGGAAAACAGCCTCGACCAGGTGGCAAATCACGAAGCAGAGTGG AAAACTGTACTGGATCACTTCTTCTCGGATTTCACCCAGCAGTTAGATAAAGCTGAAAAAGATCCGGAAGAGGGTGGTAT GCGCCCGAACCAGATGGTTCTGACCAGCATCGACTGCCCGACCTGTGGTCGCAAAATGGGTATTCGTACAGCGAGCACCG GGGTATTCCTTGGCTGTTCCGGCTACGCTCTGCCACCGAAAGAGCGTTGCAAAACCACCATCAACCTGGTGCCGGAAAAC GAAGTGCTGAACGTGCTGGAAGGTGAAGACGCTGAAACCAACGCGCTGCGCGCAAAACGTCGTTGCCCGAAATGCGGCAC GGCGATGGACAGCTATCTCATCGATCCGAAACGTAAGTTGCATGTCTGTGGTAATAACCCAACCTGCGACGGTTACGAGA TCGAAGAGGGCGAATTCCGCATTAAAGGTTATGACGGCCCGATCGTTGAGTGTGAAAAATGTGGTTCTGAAATGCACCTG AAAATGGGGCGTTTCGGTAAATACATGGCCTGCACCAACGAAGAGTGTAAAAACACACGTAAGATTTTACGTAACGGCGA AGTAGCACCACCGAAAGAAGATCCGGTGCCATTACCTGAGCTGCCGTGCGAAAAATCAGATGCTTATTTCGTGCTGCGTG ACGGTGCTGCCGGTGTGTTCCTGGCTGCCAACACTTTCCCGAAATCGCGTGAAACACGTGCGCCACTGGTGGAAGAGCTT TATCGCTTCCGCGACCGTCTGCCGGAAAAACTGCGTTATCTGGCCGATGCGCCACAGCAGGATCCGGAAGGTAATAAGAC CATGGTTCGCTTTAGCCGTAAAACCAAACAGCAATATGTCTCTTCGGAAAAAGACGGAAAGGCGACTGGCTGGTCAGCAT TTTATGTTGATGGCAAATGGGTTGAAGGGAAAAAATAA
Upstream 100 bases:
>100_bases ATATCCGCAGAGAGCGAGTCCATATCGGTAACTCGTTGCCAGTGGAAGGTTTATCAACGTGCGACGCATTCCTGGAAGAA TCAAATTAGGTAAGGTGAAT
Downstream 100 bases:
>100_bases CCTTTAATTTTGTCAGGTTTTTATAAACAAAGGGTCGCGAAAGCGGCCCTTTTTTATTGCATATTATTTTTTCTTCACAC CTATACACTAAGGCTATAAA
Product: DNA topoisomerase I
Products: NA
Alternate protein names: DNA topoisomerase I; Omega-protein; Relaxing enzyme; Swivelase; Untwisting enzyme [H]
Number of amino acids: Translated: 865; Mature: 864
Protein sequence:
>865_residues MGKALVIVESPAKAKTINKYLGSDYVVKSSVGHIRDLPTSGSAAKKSADSTSTKTAKKPKKDERGALVNRMGVDPWHNWE AHYEVLPGKEKVVSELKQLAEKADHIYLATDLDREGEAIAWHLREVIGGDDARYSRVVFNEITKNAIRQAFNKPGELNID RVNAQQARRFMDRVVGYMVSPLLWKKIARGLSAGRVQSVAVRLVVEREREIKAFVPEEFWEVDASTTTPSGEALALQVTH QNDKPFRPVNREQTQAAVSLLEKARYSVLEREDKPTTSKPGAPFITSTLQQAASTRLGFGVKKTMMMAQRLYEAGYITYM RTDSTNLSQDAVNMVRGYISDNFGKKYLPESPNQYASKENSQEAHEAIRPSDVNVMAESLKDMEADAQKLYQLIWRQFVA CQMTPAKYDSTTLTVGAGDFRLKARGRILRFDGWTKVMPALRKGDEDRILPAVDKGDALTLVELTPAQHFTKPPARFSEA SLVKELEKRGIGRPSTYASIISTIQDRGYVRVENRRFYAEKMGEIVTDRLEENFRELMNYDFTAQMENSLDQVANHEAEW KTVLDHFFSDFTQQLDKAEKDPEEGGMRPNQMVLTSIDCPTCGRKMGIRTASTGVFLGCSGYALPPKERCKTTINLVPEN EVLNVLEGEDAETNALRAKRRCPKCGTAMDSYLIDPKRKLHVCGNNPTCDGYEIEEGEFRIKGYDGPIVECEKCGSEMHL KMGRFGKYMACTNEECKNTRKILRNGEVAPPKEDPVPLPELPCEKSDAYFVLRDGAAGVFLAANTFPKSRETRAPLVEEL YRFRDRLPEKLRYLADAPQQDPEGNKTMVRFSRKTKQQYVSSEKDGKATGWSAFYVDGKWVEGKK
Sequences:
>Translated_865_residues MGKALVIVESPAKAKTINKYLGSDYVVKSSVGHIRDLPTSGSAAKKSADSTSTKTAKKPKKDERGALVNRMGVDPWHNWE AHYEVLPGKEKVVSELKQLAEKADHIYLATDLDREGEAIAWHLREVIGGDDARYSRVVFNEITKNAIRQAFNKPGELNID RVNAQQARRFMDRVVGYMVSPLLWKKIARGLSAGRVQSVAVRLVVEREREIKAFVPEEFWEVDASTTTPSGEALALQVTH QNDKPFRPVNREQTQAAVSLLEKARYSVLEREDKPTTSKPGAPFITSTLQQAASTRLGFGVKKTMMMAQRLYEAGYITYM RTDSTNLSQDAVNMVRGYISDNFGKKYLPESPNQYASKENSQEAHEAIRPSDVNVMAESLKDMEADAQKLYQLIWRQFVA CQMTPAKYDSTTLTVGAGDFRLKARGRILRFDGWTKVMPALRKGDEDRILPAVDKGDALTLVELTPAQHFTKPPARFSEA SLVKELEKRGIGRPSTYASIISTIQDRGYVRVENRRFYAEKMGEIVTDRLEENFRELMNYDFTAQMENSLDQVANHEAEW KTVLDHFFSDFTQQLDKAEKDPEEGGMRPNQMVLTSIDCPTCGRKMGIRTASTGVFLGCSGYALPPKERCKTTINLVPEN EVLNVLEGEDAETNALRAKRRCPKCGTAMDSYLIDPKRKLHVCGNNPTCDGYEIEEGEFRIKGYDGPIVECEKCGSEMHL KMGRFGKYMACTNEECKNTRKILRNGEVAPPKEDPVPLPELPCEKSDAYFVLRDGAAGVFLAANTFPKSRETRAPLVEEL YRFRDRLPEKLRYLADAPQQDPEGNKTMVRFSRKTKQQYVSSEKDGKATGWSAFYVDGKWVEGKK >Mature_864_residues GKALVIVESPAKAKTINKYLGSDYVVKSSVGHIRDLPTSGSAAKKSADSTSTKTAKKPKKDERGALVNRMGVDPWHNWEA HYEVLPGKEKVVSELKQLAEKADHIYLATDLDREGEAIAWHLREVIGGDDARYSRVVFNEITKNAIRQAFNKPGELNIDR VNAQQARRFMDRVVGYMVSPLLWKKIARGLSAGRVQSVAVRLVVEREREIKAFVPEEFWEVDASTTTPSGEALALQVTHQ NDKPFRPVNREQTQAAVSLLEKARYSVLEREDKPTTSKPGAPFITSTLQQAASTRLGFGVKKTMMMAQRLYEAGYITYMR TDSTNLSQDAVNMVRGYISDNFGKKYLPESPNQYASKENSQEAHEAIRPSDVNVMAESLKDMEADAQKLYQLIWRQFVAC QMTPAKYDSTTLTVGAGDFRLKARGRILRFDGWTKVMPALRKGDEDRILPAVDKGDALTLVELTPAQHFTKPPARFSEAS LVKELEKRGIGRPSTYASIISTIQDRGYVRVENRRFYAEKMGEIVTDRLEENFRELMNYDFTAQMENSLDQVANHEAEWK TVLDHFFSDFTQQLDKAEKDPEEGGMRPNQMVLTSIDCPTCGRKMGIRTASTGVFLGCSGYALPPKERCKTTINLVPENE VLNVLEGEDAETNALRAKRRCPKCGTAMDSYLIDPKRKLHVCGNNPTCDGYEIEEGEFRIKGYDGPIVECEKCGSEMHLK MGRFGKYMACTNEECKNTRKILRNGEVAPPKEDPVPLPELPCEKSDAYFVLRDGAAGVFLAANTFPKSRETRAPLVEELY RFRDRLPEKLRYLADAPQQDPEGNKTMVRFSRKTKQQYVSSEKDGKATGWSAFYVDGKWVEGKK
Specific function: The reaction catalyzed by topoisomerases leads to the conversion of one topological isomer of DNA to another [H]
COG id: COG0550
COG function: function code L; Topoisomerase IA
Gene ontology:
Cell location: Cytoplasm [C]
Metaboloic importance: Essential [C]
Operon status: Not Known
Operon components: None
Similarity: Contains 1 Toprim domain [H]
Homologues:
Organism=Homo sapiens, GI4507635, Length=514, Percent_Identity=27.6264591439689, Blast_Score=149, Evalue=1e-35, Organism=Homo sapiens, GI10835218, Length=609, Percent_Identity=25.2873563218391, Blast_Score=149, Evalue=2e-35, Organism=Escherichia coli, GI1787529, Length=865, Percent_Identity=99.6531791907514, Blast_Score=1800, Evalue=0.0, Organism=Escherichia coli, GI1788061, Length=549, Percent_Identity=23.8615664845173, Blast_Score=123, Evalue=4e-29, Organism=Caenorhabditis elegans, GI17555378, Length=505, Percent_Identity=25.7425742574257, Blast_Score=131, Evalue=1e-30, Organism=Caenorhabditis elegans, GI32563869, Length=565, Percent_Identity=24.4247787610619, Blast_Score=128, Evalue=2e-29, Organism=Drosophila melanogaster, GI24585251, Length=615, Percent_Identity=24.0650406504065, Blast_Score=136, Evalue=6e-32, Organism=Drosophila melanogaster, GI24640096, Length=508, Percent_Identity=25.9842519685039, Blast_Score=123, Evalue=7e-28,
Paralogues:
None
Copy number: NA
Swissprot (AC and ID): NA
Other databases:
- InterPro: IPR003601 - InterPro: IPR013497 - InterPro: IPR013824 - InterPro: IPR013825 - InterPro: IPR000380 - InterPro: IPR003602 - InterPro: IPR013498 - InterPro: IPR005733 - InterPro: IPR013263 - InterPro: IPR006171 [H]
Pfam domain/function: PF08272 Topo_Zn_Ribbon; PF01131 Topoisom_bac; PF01751 Toprim; PF01396 zf-C4_Topoisom [H]
EC number: =5.99.1.2 [H]
Molecular weight: Translated: 97410; Mature: 97279
Theoretical pI: Translated: 8.55; Mature: 8.55
Prosite motif: PS00396 TOPOISOMERASE_I_PROK
Important sites: NA
Signals:
None
Transmembrane regions:
None
Cys/Met content:
1.6 %Cys (Translated Protein) 2.8 %Met (Translated Protein) 4.4 %Cys+Met (Translated Protein) 1.6 %Cys (Mature Protein) 2.7 %Met (Mature Protein) 4.3 %Cys+Met (Mature Protein)
Secondary structure:
>Translated Secondary Structure MGKALVIVESPAKAKTINKYLGSDYVVKSSVGHIRDLPTSGSAAKKSADSTSTKTAKKPK CCCEEEEEECCCHHHHHHHHHCCCEEEECCCCHHHCCCCCCCHHHCCCCCCCCHHCCCCC KDERGALVNRMGVDPWHNWEAHYEVLPGKEKVVSELKQLAEKADHIYLATDLDREGEAIA CCHHHHHHHHCCCCCCCCCCCCEEECCCHHHHHHHHHHHHHHCCEEEEEECCCCCCCHHH WHLREVIGGDDARYSRVVFNEITKNAIRQAFNKPGELNIDRVNAQQARRFMDRVVGYMVS HHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHCCCCCCCEEECCHHHHHHHHHHHHHHHHH PLLWKKIARGLSAGRVQSVAVRLVVEREREIKAFVPEEFWEVDASTTTPSGEALALQVTH HHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHCCCHHHHCCCCCCCCCCCCEEEEEEEE QNDKPFRPVNREQTQAAVSLLEKARYSVLEREDKPTTSKPGAPFITSTLQQAASTRLGFG CCCCCCCCCCHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCHHHHHHHHHHHHHHCCC VKKTMMMAQRLYEAGYITYMRTDSTNLSQDAVNMVRGYISDNFGKKYLPESPNQYASKEN HHHHHHHHHHHHHCCCEEEEECCCCCCHHHHHHHHHHHHHCCCCCCCCCCCCCHHHCCCC SQEAHEAIRPSDVNVMAESLKDMEADAQKLYQLIWRQFVACQMTPAKYDSTTLTVGAGDF HHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCEEEECCCCE RLKARGRILRFDGWTKVMPALRKGDEDRILPAVDKGDALTLVELTPAQHFTKPPARFSEA EEEECCCEEEECCHHHHHHHHHCCCCCCCCCCCCCCCEEEEEEECCHHHCCCCCCCHHHH SLVKELEKRGIGRPSTYASIISTIQDRGYVRVENRRFYAEKMGEIVTDRLEENFRELMNY HHHHHHHHCCCCCCHHHHHHHHHHHHCCCEEEECCCHHHHHHHHHHHHHHHHHHHHHHCC DFTAQMENSLDQVANHEAEWKTVLDHFFSDFTQQLDKAEKDPEEGGMRPNQMVLTSIDCP CCHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHCCCHHHCCCCCCCEEEEECCCC TCGRKMGIRTASTGVFLGCSGYALPPKERCKTTINLVPENEVLNVLEGEDAETNALRAKR CCCHHHCCEEECCCEEEECCCCCCCCHHHCCCEEEECCCHHHHHHHCCCCCHHHHHHHHH RCPKCGTAMDSYLIDPKRKLHVCGNNPTCDGYEIEEGEFRIKGYDGPIVECEKCGSEMHL CCCCCCCHHHHHHCCCCCEEEEECCCCCCCCEEECCCCEEEECCCCCCHHHHHCCCHHHH KMGRFGKYMACTNEECKNTRKILRNGEVAPPKEDPVPLPELPCEKSDAYFVLRDGAAGVF HHHCCCCEEECCCHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCCCCEEEEECCCCEEE LAANTFPKSRETRAPLVEELYRFRDRLPEKLRYLADAPQQDPEGNKTMVRFSRKTKQQYV EEECCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCHHHHHHHHHHHHHHH SSEKDGKATGWSAFYVDGKWVEGKK CCCCCCCCCCEEEEEECCEECCCCC >Mature Secondary Structure GKALVIVESPAKAKTINKYLGSDYVVKSSVGHIRDLPTSGSAAKKSADSTSTKTAKKPK CCEEEEEECCCHHHHHHHHHCCCEEEECCCCHHHCCCCCCCHHHCCCCCCCCHHCCCCC KDERGALVNRMGVDPWHNWEAHYEVLPGKEKVVSELKQLAEKADHIYLATDLDREGEAIA CCHHHHHHHHCCCCCCCCCCCCEEECCCHHHHHHHHHHHHHHCCEEEEEECCCCCCCHHH WHLREVIGGDDARYSRVVFNEITKNAIRQAFNKPGELNIDRVNAQQARRFMDRVVGYMVS HHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHCCCCCCCEEECCHHHHHHHHHHHHHHHHH PLLWKKIARGLSAGRVQSVAVRLVVEREREIKAFVPEEFWEVDASTTTPSGEALALQVTH HHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHCCCHHHHCCCCCCCCCCCCEEEEEEEE QNDKPFRPVNREQTQAAVSLLEKARYSVLEREDKPTTSKPGAPFITSTLQQAASTRLGFG CCCCCCCCCCHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCHHHHHHHHHHHHHHCCC VKKTMMMAQRLYEAGYITYMRTDSTNLSQDAVNMVRGYISDNFGKKYLPESPNQYASKEN HHHHHHHHHHHHHCCCEEEEECCCCCCHHHHHHHHHHHHHCCCCCCCCCCCCCHHHCCCC SQEAHEAIRPSDVNVMAESLKDMEADAQKLYQLIWRQFVACQMTPAKYDSTTLTVGAGDF HHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCEEEECCCCE RLKARGRILRFDGWTKVMPALRKGDEDRILPAVDKGDALTLVELTPAQHFTKPPARFSEA EEEECCCEEEECCHHHHHHHHHCCCCCCCCCCCCCCCEEEEEEECCHHHCCCCCCCHHHH SLVKELEKRGIGRPSTYASIISTIQDRGYVRVENRRFYAEKMGEIVTDRLEENFRELMNY HHHHHHHHCCCCCCHHHHHHHHHHHHCCCEEEECCCHHHHHHHHHHHHHHHHHHHHHHCC DFTAQMENSLDQVANHEAEWKTVLDHFFSDFTQQLDKAEKDPEEGGMRPNQMVLTSIDCP CCHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHCCCHHHCCCCCCCEEEEECCCC TCGRKMGIRTASTGVFLGCSGYALPPKERCKTTINLVPENEVLNVLEGEDAETNALRAKR CCCHHHCCEEECCCEEEECCCCCCCCHHHCCCEEEECCCHHHHHHHCCCCCHHHHHHHHH RCPKCGTAMDSYLIDPKRKLHVCGNNPTCDGYEIEEGEFRIKGYDGPIVECEKCGSEMHL CCCCCCCHHHHHHCCCCCEEEEECCCCCCCCEEECCCCEEEECCCCCCHHHHHCCCHHHH KMGRFGKYMACTNEECKNTRKILRNGEVAPPKEDPVPLPELPCEKSDAYFVLRDGAAGVF HHHCCCCEEECCCHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCCCCEEEEECCCCEEE LAANTFPKSRETRAPLVEELYRFRDRLPEKLRYLADAPQQDPEGNKTMVRFSRKTKQQYV EEECCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCHHHHHHHHHHHHHHH SSEKDGKATGWSAFYVDGKWVEGKK CCCCCCCCCCEEEEEECCEECCCCC
PDB accession: NA
Resolution: NA
Structure class: Unstructured
Cofactors: NA
Metal ions: NA
Kcat value (1/min): NA
Specific activity: NA
Km value (mM): NA
Substrates: NA
Specific reaction: NA
General reaction: NA
Inhibitor: NA
Structure determination priority: 9.0
TargetDB status: NA
Availability: NA
References: 3029379; 9097039; 9278503; 3032952; 8114910; 7779808 [H]