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The map label for this gene is oppB

Identifier: 218699950

GI number: 218699950

Start: 1652327

End: 1653247

Strand: Direct

Name: oppB

Synonym: ECIAI39_1579

Alternate gene names: 218699950

Gene position: 1652327-1653247 (Clockwise)

Preceding gene: 218699949

Following gene: 218699951

Centisome position: 32.2

GC content: 46.04

Gene sequence:

>921_bases
ATGTTAAAATTTATTCTACGTCGCTGTCTGGAAGCGATTCCGACGCTATTTATTCTTATTACTATTTCGTTCTTTATGAT
GCGCCTCGCACCGGGCAGTCCTTTTACCGGCGAACGTACTTTACCACCGGAAGTGATGGCCAATATCGAAGCGAAATATC
ATCTTAATGATCCGATCATGACACAGTATTTCAGCTACCTGAAACAACTGGCGCATGGCGATTTCGGTCCATCGTTTAAA
TATAAAGATTATTCGGTCAATGACCTGGTTGCATCCAGTTTTCCCGTTTCTGCCAAACTGGGAGCCGCAGCATTTTTTCT
TGCGGTAATACTGGGTGTTAGTGCTGGCGTTATTGCCGCATTAAAGCAAAACACCAAATGGGACTATACCGTGATGGGGC
TGGCAATGACCGGGGTTGTTATCCCCAGTTTTGTGGTTGCGCCATTATTAGTCATGATATTTGCGATCATTTTGCATTGG
CTGCCGGGCGGTGGCTGGAATGGTGGAGCGCTTAAATTCATGATATTGCCAATGGTGGCGTTATCACTCGCCTATATCGC
CAGTATTGCGCGTATTACCCGTGGCTCTATGATTGAGGTTTTACACTCCAACTTTATTCGTACTGCCCGGGCGAAAGGGT
TACCTATGCGGCGGATCATTTTACGCCACGCATTAAAACCTGCTCTGTTACCCGTGCTCTCCTATATGGGGCCCGCATTT
GTCGGCATTATTACCGGTTCTATGGTTATCGAAACCATTTATGGTTTGCCGGGGATTGGGCAACTGTTCGTTAATGGCGC
ATTGAACCGTGACTATTCCTTAGTGTTAAGTCTGACCATTCTGGTTGGCGCGTTAACCATTTTGTTTAATGCCATTGTCG
ATGTGCTATATGCGGTTATCGACCCGAAAATACGTTACTGA

Upstream 100 bases:

>100_bases
ATTGTGAAGCACTAATGGCAATACGTGGGGCAGGAGTGTCCTGCTCCACGGTGTCTGATTTTTATCGCATTACAGAAGGC
ACAGGCCAGAAGGTAGGGCA

Downstream 100 bases:

>100_bases
TACTGGAGCTCGCGATGATGTTAAGTAAGAAAAACAGCGAGACGCTGGAAAATTTCAGTGAAAAGCTGGAGGTCGAAGGG
CGCAGCTTGTGGCAGGACGC

Product: oligopeptide transporter permease

Products: ADP; phosphate; oligopeptides [Cytoplasm] [C]

Alternate protein names: NA

Number of amino acids: Translated: 306; Mature: 306

Protein sequence:

>306_residues
MLKFILRRCLEAIPTLFILITISFFMMRLAPGSPFTGERTLPPEVMANIEAKYHLNDPIMTQYFSYLKQLAHGDFGPSFK
YKDYSVNDLVASSFPVSAKLGAAAFFLAVILGVSAGVIAALKQNTKWDYTVMGLAMTGVVIPSFVVAPLLVMIFAIILHW
LPGGGWNGGALKFMILPMVALSLAYIASIARITRGSMIEVLHSNFIRTARAKGLPMRRIILRHALKPALLPVLSYMGPAF
VGIITGSMVIETIYGLPGIGQLFVNGALNRDYSLVLSLTILVGALTILFNAIVDVLYAVIDPKIRY

Sequences:

>Translated_306_residues
MLKFILRRCLEAIPTLFILITISFFMMRLAPGSPFTGERTLPPEVMANIEAKYHLNDPIMTQYFSYLKQLAHGDFGPSFK
YKDYSVNDLVASSFPVSAKLGAAAFFLAVILGVSAGVIAALKQNTKWDYTVMGLAMTGVVIPSFVVAPLLVMIFAIILHW
LPGGGWNGGALKFMILPMVALSLAYIASIARITRGSMIEVLHSNFIRTARAKGLPMRRIILRHALKPALLPVLSYMGPAF
VGIITGSMVIETIYGLPGIGQLFVNGALNRDYSLVLSLTILVGALTILFNAIVDVLYAVIDPKIRY
>Mature_306_residues
MLKFILRRCLEAIPTLFILITISFFMMRLAPGSPFTGERTLPPEVMANIEAKYHLNDPIMTQYFSYLKQLAHGDFGPSFK
YKDYSVNDLVASSFPVSAKLGAAAFFLAVILGVSAGVIAALKQNTKWDYTVMGLAMTGVVIPSFVVAPLLVMIFAIILHW
LPGGGWNGGALKFMILPMVALSLAYIASIARITRGSMIEVLHSNFIRTARAKGLPMRRIILRHALKPALLPVLSYMGPAF
VGIITGSMVIETIYGLPGIGQLFVNGALNRDYSLVLSLTILVGALTILFNAIVDVLYAVIDPKIRY

Specific function: Part of the binding-protein-dependent transport system for oligopeptides; probably responsible for the translocation of the substrate across the membrane

COG id: COG0601

COG function: function code EP; ABC-type dipeptide/oligopeptide/nickel transport systems, permease components

Gene ontology:

Cell location: Cell inner membrane; Multi-pass membrane protein

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 ABC transmembrane type-1 domain

Homologues:

Organism=Escherichia coli, GI1787497, Length=306, Percent_Identity=100, Blast_Score=612, Evalue=1e-176,
Organism=Escherichia coli, GI1789965, Length=340, Percent_Identity=35.2941176470588, Blast_Score=194, Evalue=7e-51,
Organism=Escherichia coli, GI1787053, Length=308, Percent_Identity=35.7142857142857, Blast_Score=193, Evalue=1e-50,
Organism=Escherichia coli, GI1789888, Length=310, Percent_Identity=34.5161290322581, Blast_Score=176, Evalue=3e-45,
Organism=Escherichia coli, GI1788504, Length=362, Percent_Identity=26.5193370165746, Blast_Score=135, Evalue=3e-33,
Organism=Escherichia coli, GI1787761, Length=328, Percent_Identity=30.4878048780488, Blast_Score=128, Evalue=4e-31,
Organism=Escherichia coli, GI1787550, Length=331, Percent_Identity=28.0966767371601, Blast_Score=122, Evalue=3e-29,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): OPPB_ECO57 (P0AFH4)

Other databases:

- EMBL:   AE005174
- EMBL:   BA000007
- PIR:   H90846
- RefSeq:   NP_287487.1
- RefSeq:   NP_309771.1
- ProteinModelPortal:   P0AFH4
- EnsemblBacteria:   EBESCT00000024017
- EnsemblBacteria:   EBESCT00000059403
- GeneID:   913107
- GeneID:   960494
- GenomeReviews:   AE005174_GR
- GenomeReviews:   BA000007_GR
- KEGG:   ece:Z2020
- KEGG:   ecs:ECs1744
- GeneTree:   EBGT00070000031776
- HOGENOM:   HBG724229
- OMA:   IAFNAIV
- ProtClustDB:   PRK09471
- BioCyc:   ECOL83334:ECS1744-MONOMER
- InterPro:   IPR000515

Pfam domain/function: PF00528 BPD_transp_1

EC number: NA

Molecular weight: Translated: 33444; Mature: 33444

Theoretical pI: Translated: 10.19; Mature: 10.19

Prosite motif: PS50928 ABC_TM1

Important sites: NA

Signals:

None

Transmembrane regions:

HASH(0x211dcd4c)-; HASH(0x22260a3c)-; HASH(0x2230fc90)-; HASH(0x223202d4)-; HASH(0x2230fe7c)-; HASH(0x2202a948)-;

Cys/Met content:

0.3 %Cys     (Translated Protein)
4.6 %Met     (Translated Protein)
4.9 %Cys+Met (Translated Protein)
0.3 %Cys     (Mature Protein)
4.6 %Met     (Mature Protein)
4.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MLKFILRRCLEAIPTLFILITISFFMMRLAPGSPFTGERTLPPEVMANIEAKYHLNDPIM
CHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCHHHHHCCCCEEECCCHHH
TQYFSYLKQLAHGDFGPSFKYKDYSVNDLVASSFPVSAKLGAAAFFLAVILGVSAGVIAA
HHHHHHHHHHHCCCCCCCCCCCCCCHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHH
LKQNTKWDYTVMGLAMTGVVIPSFVVAPLLVMIFAIILHWLPGGGWNGGALKFMILPMVA
HHCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHH
LSLAYIASIARITRGSMIEVLHSNFIRTARAKGLPMRRIILRHALKPALLPVLSYMGPAF
HHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHH
VGIITGSMVIETIYGLPGIGQLFVNGALNRDYSLVLSLTILVGALTILFNAIVDVLYAVI
HHHHHHHHHHHHHHCCCCHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
DPKIRY
CCCCCH
>Mature Secondary Structure
MLKFILRRCLEAIPTLFILITISFFMMRLAPGSPFTGERTLPPEVMANIEAKYHLNDPIM
CHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCHHHHHCCCCEEECCCHHH
TQYFSYLKQLAHGDFGPSFKYKDYSVNDLVASSFPVSAKLGAAAFFLAVILGVSAGVIAA
HHHHHHHHHHHCCCCCCCCCCCCCCHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHH
LKQNTKWDYTVMGLAMTGVVIPSFVVAPLLVMIFAIILHWLPGGGWNGGALKFMILPMVA
HHCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHH
LSLAYIASIARITRGSMIEVLHSNFIRTARAKGLPMRRIILRHALKPALLPVLSYMGPAF
HHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHH
VGIITGSMVIETIYGLPGIGQLFVNGALNRDYSLVLSLTILVGALTILFNAIVDVLYAVI
HHHHHHHHHHHHHHCCCCHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
DPKIRY
CCCCCH

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: ATP; oligopeptides [Periplasm]; H2O [C]

Specific reaction: ATP + oligopeptides [Periplasm] + H2O = ADP + phosphate + oligopeptides [Cytoplasm] [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 7.0

TargetDB status: NA

Availability: NA

References: 11206551; 11258796