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The map label for this gene is tqsA

Identifier: 218699833

GI number: 218699833

Start: 1533558

End: 1534592

Strand: Reverse

Name: tqsA

Synonym: ECIAI39_1457

Alternate gene names: 218699833

Gene position: 1534592-1533558 (Counterclockwise)

Preceding gene: 218699836

Following gene: 218699830

Centisome position: 29.9

GC content: 49.66

Gene sequence:

>1035_bases
ATGGCAAAGCCGATCATCACGCTCAATGGCCTAAAAATCGTTATTATGTTGGGAATGCTGGTCATTATTCTCTGCGGTAT
CCGTTTTGCCGCCGAGATCATCGTGCCGTTTATTCTCGCATTATTTATTGCTGTTATTCTTAACCCGCTGGTGCAACACA
TGGTCCGCTGGCGAGTGCCGCGTGTACTGGCGGTGTCGATTTTGATGACCATCATCGTGATGGCTATGGTGTTGCTGTTA
GCTTATCTGGGTTCCGCGCTCAACGAGTTGACGCGGACGTTACCGCAATATCGCAACTCTATTATGACGCCGCTGCAAGC
TCTTGAGCCGTTGTTGCAACGCGTAGGGATTGACGTCTCAGTTGACCAACTGGCGCATTACATTGATCCGAACGCGGCGA
TGACGTTGCTCACTAACTTATTGACGCAGTTATCTAATGCCATGTCATCAATATTTTTATTGCTGCTGACGGTGTTGTTT
ATGCTGCTCGAAGTGCCACAATTGCCCGGAAAATTTCAGCAAATGATGGCGCGTCCGGTTGAAGGGATGGCGGCGATTCA
GCGTGCGATTGACAGTGTGTCTCATTATCTGGTGCTGAAAACAGCCATCAGCATCATCACCGGCCTGGTCGCATGGGCGA
TGCTCGCCGCCCTCGATGTTCGCTTCGCTTTTGTCTGGGGATTGCTGGCCTTTGCGCTTAATTACATCCCTAATATTGGT
TCAGTTCTCGCGGCAATCCCCCCTATCGCTCAGGTACTGGTGTTTAATGGCTTCTACGAAGCGTTGCTGGTGCTGGCGGG
ATATCTGCTAATTAATCTGGTCTTCGGCAATATTCTGGAGCCGCGCATCATGGGACGTGGGCTGGGGCTTTCCACATTGG
TGGTATTTTTGTCGTTGATTTTTTGGGGATGGTTGTTAGGACCGGTGGGTATGCTGCTTTCCGTGCCGTTAACAATTATT
GTCAAAATTGCACTTGAACAAACAGCGGGAGGTCAAAGCATCGCCGTTCTGTTAAGCGATCTCAATAAAGAGTGA

Upstream 100 bases:

>100_bases
AAATATTTTCACTTCTTTACCTGAGCGGTTTGATTTTCGTTATGATGACGGAGCGAAAAAGACATTATTATTAGCAAAGG
AAGAAAAAACGGGGACAAGC

Downstream 100 bases:

>100_bases
CGGCCTCCGCAGAGGCCGTCAGGGTTACAGAGCTTTCAGGATTGCATCCACGCTGGCTTTGGCGTCACCAAACAGCATGT
GGGTGTTTTCCTTGAAGAAC

Product: putative transport protein

Products: NA

Alternate protein names: Transport of quorum-sensing signal protein

Number of amino acids: Translated: 344; Mature: 343

Protein sequence:

>344_residues
MAKPIITLNGLKIVIMLGMLVIILCGIRFAAEIIVPFILALFIAVILNPLVQHMVRWRVPRVLAVSILMTIIVMAMVLLL
AYLGSALNELTRTLPQYRNSIMTPLQALEPLLQRVGIDVSVDQLAHYIDPNAAMTLLTNLLTQLSNAMSSIFLLLLTVLF
MLLEVPQLPGKFQQMMARPVEGMAAIQRAIDSVSHYLVLKTAISIITGLVAWAMLAALDVRFAFVWGLLAFALNYIPNIG
SVLAAIPPIAQVLVFNGFYEALLVLAGYLLINLVFGNILEPRIMGRGLGLSTLVVFLSLIFWGWLLGPVGMLLSVPLTII
VKIALEQTAGGQSIAVLLSDLNKE

Sequences:

>Translated_344_residues
MAKPIITLNGLKIVIMLGMLVIILCGIRFAAEIIVPFILALFIAVILNPLVQHMVRWRVPRVLAVSILMTIIVMAMVLLL
AYLGSALNELTRTLPQYRNSIMTPLQALEPLLQRVGIDVSVDQLAHYIDPNAAMTLLTNLLTQLSNAMSSIFLLLLTVLF
MLLEVPQLPGKFQQMMARPVEGMAAIQRAIDSVSHYLVLKTAISIITGLVAWAMLAALDVRFAFVWGLLAFALNYIPNIG
SVLAAIPPIAQVLVFNGFYEALLVLAGYLLINLVFGNILEPRIMGRGLGLSTLVVFLSLIFWGWLLGPVGMLLSVPLTII
VKIALEQTAGGQSIAVLLSDLNKE
>Mature_343_residues
AKPIITLNGLKIVIMLGMLVIILCGIRFAAEIIVPFILALFIAVILNPLVQHMVRWRVPRVLAVSILMTIIVMAMVLLLA
YLGSALNELTRTLPQYRNSIMTPLQALEPLLQRVGIDVSVDQLAHYIDPNAAMTLLTNLLTQLSNAMSSIFLLLLTVLFM
LLEVPQLPGKFQQMMARPVEGMAAIQRAIDSVSHYLVLKTAISIITGLVAWAMLAALDVRFAFVWGLLAFALNYIPNIGS
VLAAIPPIAQVLVFNGFYEALLVLAGYLLINLVFGNILEPRIMGRGLGLSTLVVFLSLIFWGWLLGPVGMLLSVPLTIIV
KIALEQTAGGQSIAVLLSDLNKE

Specific function: Controls the transport of the quorum-sensing signal AI-2 either by enhancing its secretion or inhibiting its uptake and consequently represses biofilm formation and motility and affects the global gene expression in biofilms

COG id: COG0628

COG function: function code R; Predicted permease

Gene ontology:

Cell location: Cell inner membrane; Multi-pass membrane protein

Metaboloic importance: Non Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the UPF0118 (perM) family

Homologues:

Organism=Escherichia coli, GI1787885, Length=344, Percent_Identity=100, Blast_Score=667, Evalue=0.0,
Organism=Escherichia coli, GI87082271, Length=339, Percent_Identity=46.0176991150442, Blast_Score=297, Evalue=6e-82,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): TQSA_ECOLI (P0AFS5)

Other databases:

- EMBL:   U00096
- EMBL:   AP009048
- PIR:   C64916
- RefSeq:   AP_002222.1
- RefSeq:   NP_416118.1
- ProteinModelPortal:   P0AFS5
- STRING:   P0AFS5
- EnsemblBacteria:   EBESCT00000004790
- EnsemblBacteria:   EBESCT00000016881
- GeneID:   946142
- GenomeReviews:   AP009048_GR
- GenomeReviews:   U00096_GR
- KEGG:   ecj:JW1593
- KEGG:   eco:b1601
- EchoBASE:   EB3687
- EcoGene:   EG13928
- eggNOG:   COG0628
- GeneTree:   EBGT00050000009277
- HOGENOM:   HBG439472
- OMA:   IALEQTS
- ProtClustDB:   PRK12287
- BioCyc:   EcoCyc:G6859-MONOMER
- Genevestigator:   P0AFS5
- GO:   GO:0006810
- InterPro:   IPR002549
- PANTHER:   PTHR21716

Pfam domain/function: PF01594 UPF0118

EC number: NA

Molecular weight: Translated: 37544; Mature: 37412

Theoretical pI: Translated: 9.35; Mature: 9.35

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

HASH(0x20ef8c1c)-; HASH(0x216eadac)-; HASH(0x21efd370)-; HASH(0x2171668c)-; HASH(0x21fc2c68)-; HASH(0x21f7bcfc)-; HASH(0x21d9ae50)-; HASH(0x1fc513fc)-; HASH(0x21f86890)-;

Cys/Met content:

0.3 %Cys     (Translated Protein)
4.9 %Met     (Translated Protein)
5.2 %Cys+Met (Translated Protein)
0.3 %Cys     (Mature Protein)
4.7 %Met     (Mature Protein)
5.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MAKPIITLNGLKIVIMLGMLVIILCGIRFAAEIIVPFILALFIAVILNPLVQHMVRWRVP
CCCCCEEECHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
RVLAVSILMTIIVMAMVLLLAYLGSALNELTRTLPQYRNSIMTPLQALEPLLQRVGIDVS
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCC
VDQLAHYIDPNAAMTLLTNLLTQLSNAMSSIFLLLLTVLFMLLEVPQLPGKFQQMMARPV
HHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHCCH
EGMAAIQRAIDSVSHYLVLKTAISIITGLVAWAMLAALDVRFAFVWGLLAFALNYIPNIG
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHH
SVLAAIPPIAQVLVFNGFYEALLVLAGYLLINLVFGNILEPRIMGRGLGLSTLVVFLSLI
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHH
FWGWLLGPVGMLLSVPLTIIVKIALEQTAGGQSIAVLLSDLNKE
HHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHCCH
>Mature Secondary Structure 
AKPIITLNGLKIVIMLGMLVIILCGIRFAAEIIVPFILALFIAVILNPLVQHMVRWRVP
CCCCEEECHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
RVLAVSILMTIIVMAMVLLLAYLGSALNELTRTLPQYRNSIMTPLQALEPLLQRVGIDVS
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCC
VDQLAHYIDPNAAMTLLTNLLTQLSNAMSSIFLLLLTVLFMLLEVPQLPGKFQQMMARPV
HHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHCCH
EGMAAIQRAIDSVSHYLVLKTAISIITGLVAWAMLAALDVRFAFVWGLLAFALNYIPNIG
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHH
SVLAAIPPIAQVLVFNGFYEALLVLAGYLLINLVFGNILEPRIMGRGLGLSTLVVFLSLI
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHH
FWGWLLGPVGMLLSVPLTIIVKIALEQTAGGQSIAVLLSDLNKE
HHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHCCH

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 7.0

TargetDB status: NA

Availability: NA

References: 9097039; 9278503