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The map label for this gene is manA

Identifier: 218699821

GI number: 218699821

Start: 1518753

End: 1519928

Strand: Reverse

Name: manA

Synonym: ECIAI39_1445

Alternate gene names: 218699821

Gene position: 1519928-1518753 (Counterclockwise)

Preceding gene: 218699824

Following gene: 218699820

Centisome position: 29.62

GC content: 50.51

Gene sequence:

>1176_bases
ATGCAAAAACTCATTAACTCAGTGCAAAACTATGCCTGGGGCAGCAAAACGGCGTTGACTGAACTTTATGGTATGGAAAA
TCCGTCCAGCCAGCCGATGGCCGAGCTGTGGATGGGCGCACATCCGAAAAGCAGTTCACGAGTGCAGAATGCCGCCGGAG
ATATCGTTTCACTGCGTGATGTGATTGAGAGTGATAAAGCGACTCTTTTGGGAGATGCCGTTGCCAAACGCTTTGGCGAA
CTGCCTTTCCTGTTCAAAGTATTATGCGCAGCACAGCCGCTCTCCATTCAGGTTCATCCAAACAAACGCAATTCTGAAAT
CGGTTTTGCCAAAGAAAATGCCGCAGGTATTCCGATGGATGCCGCCGAGCGTAACTATAAAGATCCTAACCACAAGCCGG
AGCTGGTTTTTGCGCTGACGCCTTTCCTTGCGATGAACGCGTTTCGTGAATTTTCCGAGATTGTCTCTCTGCTCCAGCCA
GTCGCAGGTGCACATCCGGCAATTGCTCACTTTTTACAACAGCCTGATGCCGAACGTTTAAGCGAACTGTTCGCCAGCCT
GTTGAATATGCAGGGTGAAGAAAAATCCCGCGCGCTGGCGATTTTAAAATCGGCCCTTGACAGCCAGCAGGGTGAACCGT
GGCAAACGATTCGTTTAATTTCTGAATTTTACCCGGAAGACAGCGGTCTGTTCTCCCCGCTATTGCTGAATGTGGTGAAA
TTGAACCCTGGCGAAGCGATGTTCCTGTTCGCTGAAACACCGCACGCTTACCTGCAAGGCGTGGCGCTGGAAGTGATGGC
GAACTCCGATAACGTGCTGCGTGCGGGTCTGACGCCTAAATACATTGATATTCCGGAACTGGTTGCCAATGTGAAGTTCG
AAGCCAAACCGGCTAACCAGTTGTTGACCCAGCCGGTGAAACAAGGTGCAGAACTGGACTTCCCGATTCCTGTTGATGAT
TTTGCCTTCTCGCTGCATGATCTTAGTGATAAAGAAACCAACATCAGCCAGCAGAGTGCCGCCATTTTGTTCTGCGTCGA
AGGCGATGCAACGCTGTGTAAAGGTTCTCAGCAGTTACAGCTTAAACCGGGTGAATCAGCGTTTATTGCCGCCAACGAAT
CACCGGTGACTGTCAAAGGCCACGGCCGTTTAGCGCGTGTTTACAACAAGCTGTAA

Upstream 100 bases:

>100_bases
AAACTGAGACTAGTACGACTTTTTGCGGCTCCAGGTTACTTCCCGTAGGATTCTTGCTTTAATAGCGGGATTAATTTCCA
CATTAAAACAGGGATTGATC

Downstream 100 bases:

>100_bases
GAGCTTACTGAAAAAATTAACATCTCTTGCTAAGCTTATTAAAGGCTTATAACACCTTCAGGCGGCCAGTCCGCCTGATT
TCATTTTATGGATAATCATT

Product: mannose-6-phosphate isomerase

Products: NA

Alternate protein names: Phosphohexomutase; Phosphomannose isomerase; PMI [H]

Number of amino acids: Translated: 391; Mature: 391

Protein sequence:

>391_residues
MQKLINSVQNYAWGSKTALTELYGMENPSSQPMAELWMGAHPKSSSRVQNAAGDIVSLRDVIESDKATLLGDAVAKRFGE
LPFLFKVLCAAQPLSIQVHPNKRNSEIGFAKENAAGIPMDAAERNYKDPNHKPELVFALTPFLAMNAFREFSEIVSLLQP
VAGAHPAIAHFLQQPDAERLSELFASLLNMQGEEKSRALAILKSALDSQQGEPWQTIRLISEFYPEDSGLFSPLLLNVVK
LNPGEAMFLFAETPHAYLQGVALEVMANSDNVLRAGLTPKYIDIPELVANVKFEAKPANQLLTQPVKQGAELDFPIPVDD
FAFSLHDLSDKETNISQQSAAILFCVEGDATLCKGSQQLQLKPGESAFIAANESPVTVKGHGRLARVYNKL

Sequences:

>Translated_391_residues
MQKLINSVQNYAWGSKTALTELYGMENPSSQPMAELWMGAHPKSSSRVQNAAGDIVSLRDVIESDKATLLGDAVAKRFGE
LPFLFKVLCAAQPLSIQVHPNKRNSEIGFAKENAAGIPMDAAERNYKDPNHKPELVFALTPFLAMNAFREFSEIVSLLQP
VAGAHPAIAHFLQQPDAERLSELFASLLNMQGEEKSRALAILKSALDSQQGEPWQTIRLISEFYPEDSGLFSPLLLNVVK
LNPGEAMFLFAETPHAYLQGVALEVMANSDNVLRAGLTPKYIDIPELVANVKFEAKPANQLLTQPVKQGAELDFPIPVDD
FAFSLHDLSDKETNISQQSAAILFCVEGDATLCKGSQQLQLKPGESAFIAANESPVTVKGHGRLARVYNKL
>Mature_391_residues
MQKLINSVQNYAWGSKTALTELYGMENPSSQPMAELWMGAHPKSSSRVQNAAGDIVSLRDVIESDKATLLGDAVAKRFGE
LPFLFKVLCAAQPLSIQVHPNKRNSEIGFAKENAAGIPMDAAERNYKDPNHKPELVFALTPFLAMNAFREFSEIVSLLQP
VAGAHPAIAHFLQQPDAERLSELFASLLNMQGEEKSRALAILKSALDSQQGEPWQTIRLISEFYPEDSGLFSPLLLNVVK
LNPGEAMFLFAETPHAYLQGVALEVMANSDNVLRAGLTPKYIDIPELVANVKFEAKPANQLLTQPVKQGAELDFPIPVDD
FAFSLHDLSDKETNISQQSAAILFCVEGDATLCKGSQQLQLKPGESAFIAANESPVTVKGHGRLARVYNKL

Specific function: Involved in the conversion of glucose to GDP-L-fucose, which can be converted to L-fucose, a capsular polysaccharide [H]

COG id: COG1482

COG function: function code G; Phosphomannose isomerase

Gene ontology:

Cell location: Cytoplasm (Probable) [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the mannose-6-phosphate isomerase type 1 family [H]

Homologues:

Organism=Homo sapiens, GI4505235, Length=406, Percent_Identity=34.2364532019704, Blast_Score=187, Evalue=2e-47,
Organism=Escherichia coli, GI1787899, Length=391, Percent_Identity=98.7212276214834, Blast_Score=788, Evalue=0.0,
Organism=Caenorhabditis elegans, GI71997620, Length=394, Percent_Identity=31.2182741116751, Blast_Score=164, Evalue=6e-41,
Organism=Caenorhabditis elegans, GI17557650, Length=419, Percent_Identity=28.8782816229117, Blast_Score=130, Evalue=1e-30,
Organism=Saccharomyces cerevisiae, GI6320839, Length=415, Percent_Identity=31.566265060241, Blast_Score=174, Evalue=2e-44,
Organism=Drosophila melanogaster, GI21356061, Length=400, Percent_Identity=31.5, Blast_Score=159, Evalue=3e-39,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR011051
- InterPro:   IPR001250
- InterPro:   IPR016305
- InterPro:   IPR018050
- InterPro:   IPR014710 [H]

Pfam domain/function: PF01238 PMI_typeI [H]

EC number: =5.3.1.8 [H]

Molecular weight: Translated: 42769; Mature: 42769

Theoretical pI: Translated: 5.13; Mature: 5.13

Prosite motif: PS00965 PMI_I_1 ; PS00966 PMI_I_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.8 %Cys     (Translated Protein)
2.3 %Met     (Translated Protein)
3.1 %Cys+Met (Translated Protein)
0.8 %Cys     (Mature Protein)
2.3 %Met     (Mature Protein)
3.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MQKLINSVQNYAWGSKTALTELYGMENPSSQPMAELWMGAHPKSSSRVQNAAGDIVSLRD
CHHHHHHHHHHCCCCHHHHHHHHCCCCCCCCCHHHHHCCCCCCCHHHHHHHHCCHHHHHH
VIESDKATLLGDAVAKRFGELPFLFKVLCAAQPLSIQVHPNKRNSEIGFAKENAAGIPMD
HHHCCCHHHHHHHHHHHHCCCHHHHHHHHCCCCCEEEECCCCCCCCCCCCCCCCCCCCCC
AAERNYKDPNHKPELVFALTPFLAMNAFREFSEIVSLLQPVAGAHPAIAHFLQQPDAERL
HHHCCCCCCCCCCCEEEEHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHCCCHHHH
SELFASLLNMQGEEKSRALAILKSALDSQQGEPWQTIRLISEFYPEDSGLFSPLLLNVVK
HHHHHHHHCCCCCHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHCCCCCCCHHHHHHHHHE
LNPGEAMFLFAETPHAYLQGVALEVMANSDNVLRAGLTPKYIDIPELVANVKFEAKPANQ
ECCCCEEEEEECCCHHHHCCEEEEEEECCCCEEECCCCCCCCCHHHHHHCCCCCCCCHHH
LLTQPVKQGAELDFPIPVDDFAFSLHDLSDKETNISQQSAAILFCVEGDATLCKGSQQLQ
HHHHHHHCCCCCCCCCCHHHHHHHHHCCCCCCCCCCCCCCEEEEEECCCHHHHCCCCEEE
LKPGESAFIAANESPVTVKGHGRLARVYNKL
ECCCCCEEEEECCCCEEEECCCHHHHHHHCC
>Mature Secondary Structure
MQKLINSVQNYAWGSKTALTELYGMENPSSQPMAELWMGAHPKSSSRVQNAAGDIVSLRD
CHHHHHHHHHHCCCCHHHHHHHHCCCCCCCCCHHHHHCCCCCCCHHHHHHHHCCHHHHHH
VIESDKATLLGDAVAKRFGELPFLFKVLCAAQPLSIQVHPNKRNSEIGFAKENAAGIPMD
HHHCCCHHHHHHHHHHHHCCCHHHHHHHHCCCCCEEEECCCCCCCCCCCCCCCCCCCCCC
AAERNYKDPNHKPELVFALTPFLAMNAFREFSEIVSLLQPVAGAHPAIAHFLQQPDAERL
HHHCCCCCCCCCCCEEEEHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHCCCHHHH
SELFASLLNMQGEEKSRALAILKSALDSQQGEPWQTIRLISEFYPEDSGLFSPLLLNVVK
HHHHHHHHCCCCCHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHCCCCCCCHHHHHHHHHE
LNPGEAMFLFAETPHAYLQGVALEVMANSDNVLRAGLTPKYIDIPELVANVKFEAKPANQ
ECCCCEEEEEECCCHHHHCCEEEEEEECCCCEEECCCCCCCCCHHHHHHCCCCCCCCHHH
LLTQPVKQGAELDFPIPVDDFAFSLHDLSDKETNISQQSAAILFCVEGDATLCKGSQQLQ
HHHHHHHCCCCCCCCCCHHHHHHHHHCCCCCCCCCCCCCCEEEEEECCCHHHHCCCCEEE
LKPGESAFIAANESPVTVKGHGRLARVYNKL
ECCCCCEEEEECCCCEEEECCCHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 6397402; 9097039; 9278503 [H]