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The map label for this gene is cfa

Identifier: 218699771

GI number: 218699771

Start: 1464687

End: 1465835

Strand: Reverse

Name: cfa

Synonym: ECIAI39_1395

Alternate gene names: 218699771

Gene position: 1465835-1464687 (Counterclockwise)

Preceding gene: 218699772

Following gene: 218699769

Centisome position: 28.56

GC content: 48.3

Gene sequence:

>1149_bases
ATGAGTTCATCGTGTATAGAAGAAGTCAGTGTACCGGATGACAACTGGTACCGTATCGCCAACGAATTACTTAGTCGTGC
CGGTATAGCCATTAACGGTTCTGCCCCGGCGGATATTCGTGTGAAAAACCCCGATTTTTTTAAACGCGTTCTGCAGGAAG
GATCGTTGGGGTTAGGCGAAAGTTATATGGATGGCTGGTGGGAATGTGACCGACTGGATATGTTTTTTAGCAAAGTCTTA
CGCGCAGGTCTCGAGAACCAACTCCCCCATCATTTCAAAGACACGCTGCGTATTGCCGGCGCTCGTCTCTTCAATCTGCA
GAGTAAAAAACGTGCCTGGATAGTCGGCAAAGAGCATTACGATTTGGGTAATGACTTGTTCAGCCGCATGCTTGATCCCT
TCATGCAATATTCCTGTGCTTACTGGAAAGATGCCGATAATCTGGAATCTGCCCAGCAGGCGAAGCTCAAAATGATTTGT
GAAAAATTGCAGTTAAAACCAGGGATGCGCGTACTGGATATTGGCTGTGGCTGGGGCGGACTGGCGCACTACATGGCGTC
TAATTATGACGTAAGCGTGGTCGGCGTAACCATTTCTGCCGAACAGCAAAAAATGGCTCAGGAACGCTGTGAAGGCCTGG
ATGTCACCATTTTGCTGCAAGATTATCGTGACCTGAACGACCAGTTTGACCGTATTGTTTCTGTGGGGATGTTCGAACAC
GTCGGACCGAAAAATTACGATACCTATTTTGCGGTGGTGGATCGTAATTTGAAACCGGAAGGTATATTCCTGCTCCATAC
CATCGGTTCGAAAAAAACCGATCTGAATGTTGATCCCTGGATTAATAAATATATTTTTCCGAACGGTTGCCTGCCCTCTG
TACGCCAGATTGCCCAGTCCAGCGAACCCCACTTTGTGATGGAAGACTGGCATAACTTCGGTGCTGATTACGATACTACG
TTGATGGCGTGGTATGAACGTTTCCTCGCCGCATGGCCAGAAATTGCGGATAACTACAGTGAACGCTTTAAACGGATGTT
TACCTATTATCTGAATGCCTGTGCAGGTGCTTTCCGCGCCCGTGATATTCAGCTCTGGCAGGTGGTGTTCTCTCGCGGAG
TCGAAAACGGCCTTCGAGTCGCTCGCTAG

Upstream 100 bases:

>100_bases
GATTATAGACTCGCGGTTTTTTCTGCGAGATTTCTCACAAAGCCCAAAAAGCGTCTACGCTGTTTTAAGGTTCTGATCAC
CGACCAGTGATGGAGAAACT

Downstream 100 bases:

>100_bases
AGGCTATTCTATCGCCCCCGCTCCGGGGGCGATTTCAGATCAGGCTTCTGTACCTGGTTGATTCATGGCATTTTCTCGTG
CCGCCAGCACACGTTCTACC

Product: cyclopropane fatty acyl phospholipid synthase

Products: NA

Alternate protein names: CFA synthase; Cyclopropane fatty acid synthase

Number of amino acids: Translated: 382; Mature: 381

Protein sequence:

>382_residues
MSSSCIEEVSVPDDNWYRIANELLSRAGIAINGSAPADIRVKNPDFFKRVLQEGSLGLGESYMDGWWECDRLDMFFSKVL
RAGLENQLPHHFKDTLRIAGARLFNLQSKKRAWIVGKEHYDLGNDLFSRMLDPFMQYSCAYWKDADNLESAQQAKLKMIC
EKLQLKPGMRVLDIGCGWGGLAHYMASNYDVSVVGVTISAEQQKMAQERCEGLDVTILLQDYRDLNDQFDRIVSVGMFEH
VGPKNYDTYFAVVDRNLKPEGIFLLHTIGSKKTDLNVDPWINKYIFPNGCLPSVRQIAQSSEPHFVMEDWHNFGADYDTT
LMAWYERFLAAWPEIADNYSERFKRMFTYYLNACAGAFRARDIQLWQVVFSRGVENGLRVAR

Sequences:

>Translated_382_residues
MSSSCIEEVSVPDDNWYRIANELLSRAGIAINGSAPADIRVKNPDFFKRVLQEGSLGLGESYMDGWWECDRLDMFFSKVL
RAGLENQLPHHFKDTLRIAGARLFNLQSKKRAWIVGKEHYDLGNDLFSRMLDPFMQYSCAYWKDADNLESAQQAKLKMIC
EKLQLKPGMRVLDIGCGWGGLAHYMASNYDVSVVGVTISAEQQKMAQERCEGLDVTILLQDYRDLNDQFDRIVSVGMFEH
VGPKNYDTYFAVVDRNLKPEGIFLLHTIGSKKTDLNVDPWINKYIFPNGCLPSVRQIAQSSEPHFVMEDWHNFGADYDTT
LMAWYERFLAAWPEIADNYSERFKRMFTYYLNACAGAFRARDIQLWQVVFSRGVENGLRVAR
>Mature_381_residues
SSSCIEEVSVPDDNWYRIANELLSRAGIAINGSAPADIRVKNPDFFKRVLQEGSLGLGESYMDGWWECDRLDMFFSKVLR
AGLENQLPHHFKDTLRIAGARLFNLQSKKRAWIVGKEHYDLGNDLFSRMLDPFMQYSCAYWKDADNLESAQQAKLKMICE
KLQLKPGMRVLDIGCGWGGLAHYMASNYDVSVVGVTISAEQQKMAQERCEGLDVTILLQDYRDLNDQFDRIVSVGMFEHV
GPKNYDTYFAVVDRNLKPEGIFLLHTIGSKKTDLNVDPWINKYIFPNGCLPSVRQIAQSSEPHFVMEDWHNFGADYDTTL
MAWYERFLAAWPEIADNYSERFKRMFTYYLNACAGAFRARDIQLWQVVFSRGVENGLRVAR

Specific function: Transfers a methylene group from S-adenosyl-L-methionine to the cis double bond of an unsaturated fatty acid chain resulting in the replacement of the double bond with a methylene bridge

COG id: COG2230

COG function: function code M; Cyclopropane fatty acid synthase and related methyltransferases

Gene ontology:

Cell location: Cytoplasm (Probable)

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the CFA/CMAS family

Homologues:

Organism=Escherichia coli, GI1787951, Length=382, Percent_Identity=100, Blast_Score=800, Evalue=0.0,
Organism=Caenorhabditis elegans, GI133904226, Length=385, Percent_Identity=30.9090909090909, Blast_Score=145, Evalue=4e-35,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): CFA_ECOL6 (P0A9H8)

Other databases:

- EMBL:   AE014075
- RefSeq:   NP_753950.1
- ProteinModelPortal:   P0A9H8
- SMR:   P0A9H8
- EnsemblBacteria:   EBESCT00000041715
- GeneID:   1036202
- GenomeReviews:   AE014075_GR
- KEGG:   ecc:c2055
- GeneTree:   EBGT00050000011613
- HOGENOM:   HBG687502
- OMA:   GPKNYAT
- ProtClustDB:   PRK11705
- BRENDA:   2.1.1.79
- GO:   GO:0005737
- InterPro:   IPR003333
- PIRSF:   PIRSF003085

Pfam domain/function: PF02353 CMAS

EC number: =2.1.1.79

Molecular weight: Translated: 43909; Mature: 43778

Theoretical pI: Translated: 5.90; Mature: 5.90

Prosite motif: NA

Important sites: ACT_SITE 354-354

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.1 %Cys     (Translated Protein)
3.4 %Met     (Translated Protein)
5.5 %Cys+Met (Translated Protein)
2.1 %Cys     (Mature Protein)
3.1 %Met     (Mature Protein)
5.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSSSCIEEVSVPDDNWYRIANELLSRAGIAINGSAPADIRVKNPDFFKRVLQEGSLGLGE
CCCHHHHHCCCCCCHHHHHHHHHHHHCCEEEECCCCCCEEECCHHHHHHHHHCCCCCCCC
SYMDGWWECDRLDMFFSKVLRAGLENQLPHHFKDTLRIAGARLFNLQSKKRAWIVGKEHY
HHHCCHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHCCCCCCCEEEECCHHC
DLGNDLFSRMLDPFMQYSCAYWKDADNLESAQQAKLKMICEKLQLKPGMRVLDIGCGWGG
CCHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHCCCCCEEEEECCCCHH
LAHYMASNYDVSVVGVTISAEQQKMAQERCEGLDVTILLQDYRDLNDQFDRIVSVGMFEH
HHHHHHCCCCEEEEEEEECHHHHHHHHHHHCCCCEEEEEHHHHHHHHHHHHHHHHHHHHH
VGPKNYDTYFAVVDRNLKPEGIFLLHTIGSKKTDLNVDPWINKYIFPNGCLPSVRQIAQS
CCCCCCCCEEEEEECCCCCCCEEEEEECCCCCCCCCCCCCHHCCCCCCCCCHHHHHHHCC
SEPHFVMEDWHNFGADYDTTLMAWYERFLAAWPEIADNYSERFKRMFTYYLNACAGAFRA
CCCCEEHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
RDIQLWQVVFSRGVENGLRVAR
HHHHHHHHHHHHCHHCCCCCCC
>Mature Secondary Structure 
SSSCIEEVSVPDDNWYRIANELLSRAGIAINGSAPADIRVKNPDFFKRVLQEGSLGLGE
CCHHHHHCCCCCCHHHHHHHHHHHHCCEEEECCCCCCEEECCHHHHHHHHHCCCCCCCC
SYMDGWWECDRLDMFFSKVLRAGLENQLPHHFKDTLRIAGARLFNLQSKKRAWIVGKEHY
HHHCCHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHCCCCCCCEEEECCHHC
DLGNDLFSRMLDPFMQYSCAYWKDADNLESAQQAKLKMICEKLQLKPGMRVLDIGCGWGG
CCHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHCCCCCEEEEECCCCHH
LAHYMASNYDVSVVGVTISAEQQKMAQERCEGLDVTILLQDYRDLNDQFDRIVSVGMFEH
HHHHHHCCCCEEEEEEEECHHHHHHHHHHHCCCCEEEEEHHHHHHHHHHHHHHHHHHHHH
VGPKNYDTYFAVVDRNLKPEGIFLLHTIGSKKTDLNVDPWINKYIFPNGCLPSVRQIAQS
CCCCCCCCEEEEEECCCCCCCEEEEEECCCCCCCCCCCCCHHCCCCCCCCCHHHHHHHCC
SEPHFVMEDWHNFGADYDTTLMAWYERFLAAWPEIADNYSERFKRMFTYYLNACAGAFRA
CCCCEEHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
RDIQLWQVVFSRGVENGLRVAR
HHHHHHHHHHHHCHHCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 12471157