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The map label for this gene is lpxM

Identifier: 218699573

GI number: 218699573

Start: 1263553

End: 1264524

Strand: Direct

Name: lpxM

Synonym: ECIAI39_1194

Alternate gene names: 218699573

Gene position: 1263553-1264524 (Clockwise)

Preceding gene: 218699572

Following gene: 218699576

Centisome position: 24.62

GC content: 51.03

Gene sequence:

>972_bases
ATGGAAACGAAAAAAAATAATAGCGAATACATTCCTGAGTTTGATAAATCCTTTCGCCACCCGCGCTACTGGGGAGCATG
GCTGGGCGTAGCAGCGATGGCTGGTATCGCTTTAACGCCGCCAAAGTTCCGTGATCCCATTCTGGCACGGCTGGGACGTT
TTGCCGGACGACTGGGAAAAAGCTCACGCCGTCGAGCGTTAATCAATCTGTCGCTCTGCTTTCCAGAACGTAGTGAAGCT
GAACGCGAAGCGATTGTTGATGAGATGTTTGCCACCGCGCCGCAAGCGATGGCAATGATGGCTGAGTTGGCAATACGCGG
GCCGGAGAAAATTCAGCCGCGCGTTGACTGGCAAGGGCTGGAGATTATCGAAGAGATGCGGCGTAATAACGAGAAAGTGA
TTTTTCTGGTGCCGCACGGTTGGGCCGTCGATATCCCTGCCATGCTGATGGCCTCGCAAGGGCAGAAAATGGCAGCGATG
TTCCATAATCAGGGCAACCCGGTTTTTGATTATGTCTGGAACACGGTGCGTCGTCGTTTTGGCGGTCGTCTGCATGCGAG
AAATGATGGTATTAAACCATTCATCCAGTCGGTACGTCAGGGGTACTGGGGATATTATTTACCCGATCAGGATCATGGCC
CAGAGCACAGCGAATTTGTTGATTTCTTTGCCACCTATAAAGCGACGTTGCCCGCGATTGGTCGTTTGATGAAAGTGTGC
CGTGCGCGCGTTGTACCGCTGTTTCCGATTTATGATGGCAAGACGCATCGCCTGACGATTCAGGTGCGCCCACCGATGGA
TGATCTGTTAGAGGCGGATGACCATACGATTGCGCGGTGGATGAATGAAGAAGTCGAGATTTTTGTTGGTCCGCGACCAG
AACAATACACCTGGATATTAAAATTGCTGAAAACTCGCAAACCGGGCGAAATCCAACCGTATAAGCGCAAAGATCTTTAT
CCCATCAAATAA

Upstream 100 bases:

>100_bases
CGGTACGCAGTCAGTACCGGCTTTTTTATTTGGTGCGGGGCAAGTTGCGCCGCTACACTATCACCAGATTGATTTTTGCC
TTATCCGAAACTGGAAAAGC

Downstream 100 bases:

>100_bases
AAAAAGCCTCTCGCGAGGAGAGGCTTCCGCCGTGTGATAAGTTCAAGTTTGCTTCAGAAGATTCGAAATCTGTTGAACTA
TCATTGAACTGTAGGCCGGA

Product: lipid A biosynthesis (KDO)2-(lauroyl)-lipid IVA acyltransferase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 323; Mature: 323

Protein sequence:

>323_residues
METKKNNSEYIPEFDKSFRHPRYWGAWLGVAAMAGIALTPPKFRDPILARLGRFAGRLGKSSRRRALINLSLCFPERSEA
EREAIVDEMFATAPQAMAMMAELAIRGPEKIQPRVDWQGLEIIEEMRRNNEKVIFLVPHGWAVDIPAMLMASQGQKMAAM
FHNQGNPVFDYVWNTVRRRFGGRLHARNDGIKPFIQSVRQGYWGYYLPDQDHGPEHSEFVDFFATYKATLPAIGRLMKVC
RARVVPLFPIYDGKTHRLTIQVRPPMDDLLEADDHTIARWMNEEVEIFVGPRPEQYTWILKLLKTRKPGEIQPYKRKDLY
PIK

Sequences:

>Translated_323_residues
METKKNNSEYIPEFDKSFRHPRYWGAWLGVAAMAGIALTPPKFRDPILARLGRFAGRLGKSSRRRALINLSLCFPERSEA
EREAIVDEMFATAPQAMAMMAELAIRGPEKIQPRVDWQGLEIIEEMRRNNEKVIFLVPHGWAVDIPAMLMASQGQKMAAM
FHNQGNPVFDYVWNTVRRRFGGRLHARNDGIKPFIQSVRQGYWGYYLPDQDHGPEHSEFVDFFATYKATLPAIGRLMKVC
RARVVPLFPIYDGKTHRLTIQVRPPMDDLLEADDHTIARWMNEEVEIFVGPRPEQYTWILKLLKTRKPGEIQPYKRKDLY
PIK
>Mature_323_residues
METKKNNSEYIPEFDKSFRHPRYWGAWLGVAAMAGIALTPPKFRDPILARLGRFAGRLGKSSRRRALINLSLCFPERSEA
EREAIVDEMFATAPQAMAMMAELAIRGPEKIQPRVDWQGLEIIEEMRRNNEKVIFLVPHGWAVDIPAMLMASQGQKMAAM
FHNQGNPVFDYVWNTVRRRFGGRLHARNDGIKPFIQSVRQGYWGYYLPDQDHGPEHSEFVDFFATYKATLPAIGRLMKVC
RARVVPLFPIYDGKTHRLTIQVRPPMDDLLEADDHTIARWMNEEVEIFVGPRPEQYTWILKLLKTRKPGEIQPYKRKDLY
PIK

Specific function: Transfers myristate or laurate, activated on ACP, to (KDO)2-(lauroyl)-lipid IVA [H]

COG id: COG1560

COG function: function code M; Lauroyl/myristoyl acyltransferase

Gene ontology:

Cell location: Cell inner membrane; Single-pass membrane protein (Potential) [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the htrB/msbB family [H]

Homologues:

Organism=Escherichia coli, GI1788161, Length=323, Percent_Identity=99.6904024767802, Blast_Score=666, Evalue=0.0,
Organism=Escherichia coli, GI1787292, Length=310, Percent_Identity=30.9677419354839, Blast_Score=126, Evalue=2e-30,
Organism=Escherichia coli, GI87082094, Length=307, Percent_Identity=26.0586319218241, Blast_Score=107, Evalue=1e-24,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR004960
- InterPro:   IPR011921 [H]

Pfam domain/function: PF03279 Lip_A_acyltrans [H]

EC number: 2.3.1.-

Molecular weight: Translated: 37441; Mature: 37441

Theoretical pI: Translated: 9.97; Mature: 9.97

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.6 %Cys     (Translated Protein)
4.3 %Met     (Translated Protein)
5.0 %Cys+Met (Translated Protein)
0.6 %Cys     (Mature Protein)
4.3 %Met     (Mature Protein)
5.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
METKKNNSEYIPEFDKSFRHPRYWGAWLGVAAMAGIALTPPKFRDPILARLGRFAGRLGK
CCCCCCCCCCCCCHHHHCCCCCHHHHHHHHHHHCCCCCCCCCCCCHHHHHHHHHHHHHCC
SSRRRALINLSLCFPERSEAEREAIVDEMFATAPQAMAMMAELAIRGPEKIQPRVDWQGL
CCCCEEEEEEEEECCCHHHHHHHHHHHHHHHCCHHHHHHHHHHHHCCCHHCCCCCCCCHH
EIIEEMRRNNEKVIFLVPHGWAVDIPAMLMASQGQKMAAMFHNQGNPVFDYVWNTVRRRF
HHHHHHHCCCCEEEEEECCCCEECHHHHHHHCCCCHHHHHHHCCCCCHHHHHHHHHHHHH
GGRLHARNDGIKPFIQSVRQGYWGYYLPDQDHGPEHSEFVDFFATYKATLPAIGRLMKVC
CCEEECCCCCCHHHHHHHHCCCCEEECCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHH
RARVVPLFPIYDGKTHRLTIQVRPPMDDLLEADDHTIARWMNEEVEIFVGPRPEQYTWIL
HHHCCEEEEEECCCEEEEEEEECCCHHHHHCCCCHHHHHHCCCCEEEEECCCCCHHHHHH
KLLKTRKPGEIQPYKRKDLYPIK
HHHHCCCCCCCCCCCCCCCCCCC
>Mature Secondary Structure
METKKNNSEYIPEFDKSFRHPRYWGAWLGVAAMAGIALTPPKFRDPILARLGRFAGRLGK
CCCCCCCCCCCCCHHHHCCCCCHHHHHHHHHHHCCCCCCCCCCCCHHHHHHHHHHHHHCC
SSRRRALINLSLCFPERSEAEREAIVDEMFATAPQAMAMMAELAIRGPEKIQPRVDWQGL
CCCCEEEEEEEEECCCHHHHHHHHHHHHHHHCCHHHHHHHHHHHHCCCHHCCCCCCCCHH
EIIEEMRRNNEKVIFLVPHGWAVDIPAMLMASQGQKMAAMFHNQGNPVFDYVWNTVRRRF
HHHHHHHCCCCEEEEEECCCCEECHHHHHHHCCCCHHHHHHHCCCCCHHHHHHHHHHHHH
GGRLHARNDGIKPFIQSVRQGYWGYYLPDQDHGPEHSEFVDFFATYKATLPAIGRLMKVC
CCEEECCCCCCHHHHHHHHCCCCEEECCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHH
RARVVPLFPIYDGKTHRLTIQVRPPMDDLLEADDHTIARWMNEEVEIFVGPRPEQYTWIL
HHHCCEEEEEECCCEEEEEEEECCCHHHHHCCCCHHHHHHCCCCEEEEECCCCCHHHHHH
KLLKTRKPGEIQPYKRKDLYPIK
HHHHCCCCCCCCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: Transferases; Acyltransferases [C]

Inhibitor: NA

Structure determination priority: 7.0

TargetDB status: NA

Availability: NA

References: 12384590; 12704152 [H]