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The map label for this gene is bglF

Identifier: 218697445

GI number: 218697445

Start: 4278889

End: 4280766

Strand: Reverse

Name: bglF

Synonym: EC55989_4192

Alternate gene names: 218697445

Gene position: 4280766-4278889 (Counterclockwise)

Preceding gene: 218697446

Following gene: 218697444

Centisome position: 83.04

GC content: 51.44

Gene sequence:

>1878_bases
ATGACGGAGTTAGCCAGAAAAATAGTCGCAGGAGTCGGGGGCGCAGATAACATTGTGAGTCTGATGCATTGCGCAACGCG
ATTACGTTTTAAGTTAAAGGATGAAAGCAAAGCGCAAGCTGAGGTACTGAAAAAGACCCCCGGTATTATTATGGTGGTGG
AAAGCGGTGGTCAGTTTCAGGTGGTCATAGGTAACCATGTGGCCGATGTCTTCCTGGCGGTCAACAGCGTGGCAGGCCTT
GGCGAAAAAGCGCAACAGGCACCGGAAAATGATGAGAAAGGTAATCTGCTAAACCGCTTTGTTTATGTTATTTCAGGTAT
TTTTACGCCTCTGATCGGTTTGATGGCGGCAACCGGGATCCTGAAAGGTATGCTGGCTCTGGCGCTCACTTTTCAGTGGA
CGACCGAACAAAGTGGTACTTATTTAATTTTATTCAGCGCCAGTGATGCCTTGTTTTGGTTCTTCCCGATAATCCTGGGA
TACACCGCGGGGAAACGCTTCGGCGGTAATCCATTTACTGCCATGGTGATTGGTGGAGCGTTAGTGCATCCATTAATTCT
GACTGCTTTCGAGAACGGGCAAAAAGCGGATGAGCTGGGGCTGGATTTCCTGGGTATTCCGGTCACATTGTTGAATTACT
CGTCATCGGTTATTCCCATTATTTTTTCTGCCTGGTTGTGCAGCATTCTGGAACGCCGACTTAATGCGTGGTTACCGTCG
GCAATCAAAAATTTCTTCACACCATTGCTATGTCTGATGGTTATCACACCCGTCACCTTTCTGCTGGTGGGGCCGCTATC
AACCTGGATAAGCGAACTGATTGCCGCCGGTTATCTCTGGCTTTATCAGGCGGTTCCTGCATTTGCAGGCGCGGTAATGG
GCGGCTTCTGGCAAATCTTCGTCATGTTCGGACTGCACTGGGGCCTGGTGCCGCTATGTATCAATAACTTCACCGTGCTG
GGCTACGACACCATGATCCCGCTGTTAATGCCCGCCATTATGGCGCAGGTCGGGGCGGCGCTCGGCGTCTTCCTCTGCGA
ACGCGATGCGCAGAAAAAAGTGGTGGCGGGATCAGCGGCGTTGACGGGCCTGTTTGGTATCACCGAACCAGCGGTATATG
GCGTTAATCTGCCGCGTAAGTACCCCTTTGTTATCGCCTGTATCAGTGGGGCTTTGGGGGCCACCATCATTGGCTACGCG
CAAACGAAAGTCTACTCCTTTGGTTTGCCAAGTATTTTCACCTTCATGCAAACCATCCCGTCAACGGGAATTGATTTCAC
CGTCTGGGCCAGCGTTATTGGCGGTGTCATTGCCATCGGTTGCGCATTTGTCGGTACGGTGATGCTTCATTTCATCACCG
CTAAACGTCAGCCAGCGCAGGGTGCCCCGCAAGAGAAAACACCAGAGGTTATTACACCACCTGAGCAGGGCGGTATCTGT
TCACCGATGACGGGAGAGATTGTGCCGCTCATTCACGTCGCTGATACCACGTTTGCCAGTGGCCTGTTGGGTAAAGGTAT
CGCCATTCTGCCCTCGGTTGGTGAAGTGCGTTCTCCGGTTGCGGGTCGAATTGCTTCGTTGTTCGCCACATTACACGCCA
TTGGCATTGAGTCAGATGATGGTGTGGAGCTCCTGATTCATGTCGGTATCGACACCGTAAAACTGGACGGCAAATTCTTT
TCCGCTCACGTCAACGTGGGTGACAAGGTCAATACAGGCGATCGGCTGATTTCTTTTGATATCCCTGCTATTCGCGAGGC
CGGATTTGATCTGACGACGCCGGTATTAATCAGTAATAGCGATGATTTTACGGACGTATTACCCCACGGCACGGCGCAGA
TAAGCGCAGGTGAACCGCTGTTATCCATCATTCGCTAA

Upstream 100 bases:

>100_bases
CTAAGCGGGCAAAACCTGAAAAAAATTGCTTGATTCACGTCAGGCCGTTTTTTTCAGGTTTTTTTTTGGAGTTTTGCCGC
AAAGCGGTAGAGGGCAAGTT

Downstream 100 bases:

>100_bases
CGATAAAAGGAGTTAATTATGAAAGCATTTCCAGAAACATTTCTTTGGGGTGGCGCAACAGCTGCCAATCAGGTGGAAGG
TGCCTGGCAGGAAGATGGCA

Product: PTS system beta-glucoside-specific transporter subunits IIABC

Products: NA

Alternate protein names: EIIBCA-Bgl; EII-Bgl; Beta-glucoside-specific phosphotransferase enzyme IIB component; PTS system beta-glucoside-specific EIIB component; Beta-glucoside permease IIC component; PTS system beta-glucoside-specific EIIC component; Beta-glucoside-specific phosphotransferase enzyme IIA component; PTS system beta-glucoside-specific EIIA component [H]

Number of amino acids: Translated: 625; Mature: 624

Protein sequence:

>625_residues
MTELARKIVAGVGGADNIVSLMHCATRLRFKLKDESKAQAEVLKKTPGIIMVVESGGQFQVVIGNHVADVFLAVNSVAGL
GEKAQQAPENDEKGNLLNRFVYVISGIFTPLIGLMAATGILKGMLALALTFQWTTEQSGTYLILFSASDALFWFFPIILG
YTAGKRFGGNPFTAMVIGGALVHPLILTAFENGQKADELGLDFLGIPVTLLNYSSSVIPIIFSAWLCSILERRLNAWLPS
AIKNFFTPLLCLMVITPVTFLLVGPLSTWISELIAAGYLWLYQAVPAFAGAVMGGFWQIFVMFGLHWGLVPLCINNFTVL
GYDTMIPLLMPAIMAQVGAALGVFLCERDAQKKVVAGSAALTGLFGITEPAVYGVNLPRKYPFVIACISGALGATIIGYA
QTKVYSFGLPSIFTFMQTIPSTGIDFTVWASVIGGVIAIGCAFVGTVMLHFITAKRQPAQGAPQEKTPEVITPPEQGGIC
SPMTGEIVPLIHVADTTFASGLLGKGIAILPSVGEVRSPVAGRIASLFATLHAIGIESDDGVELLIHVGIDTVKLDGKFF
SAHVNVGDKVNTGDRLISFDIPAIREAGFDLTTPVLISNSDDFTDVLPHGTAQISAGEPLLSIIR

Sequences:

>Translated_625_residues
MTELARKIVAGVGGADNIVSLMHCATRLRFKLKDESKAQAEVLKKTPGIIMVVESGGQFQVVIGNHVADVFLAVNSVAGL
GEKAQQAPENDEKGNLLNRFVYVISGIFTPLIGLMAATGILKGMLALALTFQWTTEQSGTYLILFSASDALFWFFPIILG
YTAGKRFGGNPFTAMVIGGALVHPLILTAFENGQKADELGLDFLGIPVTLLNYSSSVIPIIFSAWLCSILERRLNAWLPS
AIKNFFTPLLCLMVITPVTFLLVGPLSTWISELIAAGYLWLYQAVPAFAGAVMGGFWQIFVMFGLHWGLVPLCINNFTVL
GYDTMIPLLMPAIMAQVGAALGVFLCERDAQKKVVAGSAALTGLFGITEPAVYGVNLPRKYPFVIACISGALGATIIGYA
QTKVYSFGLPSIFTFMQTIPSTGIDFTVWASVIGGVIAIGCAFVGTVMLHFITAKRQPAQGAPQEKTPEVITPPEQGGIC
SPMTGEIVPLIHVADTTFASGLLGKGIAILPSVGEVRSPVAGRIASLFATLHAIGIESDDGVELLIHVGIDTVKLDGKFF
SAHVNVGDKVNTGDRLISFDIPAIREAGFDLTTPVLISNSDDFTDVLPHGTAQISAGEPLLSIIR
>Mature_624_residues
TELARKIVAGVGGADNIVSLMHCATRLRFKLKDESKAQAEVLKKTPGIIMVVESGGQFQVVIGNHVADVFLAVNSVAGLG
EKAQQAPENDEKGNLLNRFVYVISGIFTPLIGLMAATGILKGMLALALTFQWTTEQSGTYLILFSASDALFWFFPIILGY
TAGKRFGGNPFTAMVIGGALVHPLILTAFENGQKADELGLDFLGIPVTLLNYSSSVIPIIFSAWLCSILERRLNAWLPSA
IKNFFTPLLCLMVITPVTFLLVGPLSTWISELIAAGYLWLYQAVPAFAGAVMGGFWQIFVMFGLHWGLVPLCINNFTVLG
YDTMIPLLMPAIMAQVGAALGVFLCERDAQKKVVAGSAALTGLFGITEPAVYGVNLPRKYPFVIACISGALGATIIGYAQ
TKVYSFGLPSIFTFMQTIPSTGIDFTVWASVIGGVIAIGCAFVGTVMLHFITAKRQPAQGAPQEKTPEVITPPEQGGICS
PMTGEIVPLIHVADTTFASGLLGKGIAILPSVGEVRSPVAGRIASLFATLHAIGIESDDGVELLIHVGIDTVKLDGKFFS
AHVNVGDKVNTGDRLISFDIPAIREAGFDLTTPVLISNSDDFTDVLPHGTAQISAGEPLLSIIR

Specific function: Acts as both a kinase and a phosphatase on BglG [H]

COG id: COG1263

COG function: function code G; Phosphotransferase system IIC components, glucose/maltose/N-acetylglucosamine-specific

Gene ontology:

Cell location: Cell inner membrane; Multi-pass membrane protein [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 PTS EIIC type-1 domain [H]

Homologues:

Organism=Escherichia coli, GI1790159, Length=625, Percent_Identity=99.2, Blast_Score=1246, Evalue=0.0,
Organism=Escherichia coli, GI48994906, Length=457, Percent_Identity=31.0722100656455, Blast_Score=202, Evalue=5e-53,
Organism=Escherichia coli, GI1788769, Length=434, Percent_Identity=30.184331797235, Blast_Score=153, Evalue=4e-38,
Organism=Escherichia coli, GI2367362, Length=404, Percent_Identity=25.7425742574257, Blast_Score=151, Evalue=1e-37,
Organism=Escherichia coli, GI1788757, Length=146, Percent_Identity=41.7808219178082, Blast_Score=114, Evalue=2e-26,
Organism=Escherichia coli, GI1786894, Length=162, Percent_Identity=35.8024691358025, Blast_Score=106, Evalue=4e-24,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR011055
- InterPro:   IPR018113
- InterPro:   IPR004719
- InterPro:   IPR001127
- InterPro:   IPR001996
- InterPro:   IPR003352
- InterPro:   IPR013013
- InterPro:   IPR011535
- InterPro:   IPR011297 [H]

Pfam domain/function: PF00358 PTS_EIIA_1; PF00367 PTS_EIIB; PF02378 PTS_EIIC [H]

EC number: =2.7.1.69 [H]

Molecular weight: Translated: 66468; Mature: 66337

Theoretical pI: Translated: 6.01; Mature: 6.01

Prosite motif: PS00371 PTS_EIIA_TYPE_1_HIS ; PS51093 PTS_EIIA_TYPE_1 ; PS01035 PTS_EIIB_TYPE_1_CYS ; PS51098 PTS_EIIB_TYPE_1 ; PS51103 PTS_EIIC_TYPE_1

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.3 %Cys     (Translated Protein)
2.4 %Met     (Translated Protein)
3.7 %Cys+Met (Translated Protein)
1.3 %Cys     (Mature Protein)
2.2 %Met     (Mature Protein)
3.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTELARKIVAGVGGADNIVSLMHCATRLRFKLKDESKAQAEVLKKTPGIIMVVESGGQFQ
CHHHHHHHHHCCCCHHHHHHHHHHHHHHHEEECCCHHHHHHHHHCCCCEEEEEECCCEEE
VVIGNHVADVFLAVNSVAGLGEKAQQAPENDEKGNLLNRFVYVISGIFTPLIGLMAATGI
EEECCHHHHHHHHHHHHHCCCHHHHCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHH
LKGMLALALTFQWTTEQSGTYLILFSASDALFWFFPIILGYTAGKRFGGNPFTAMVIGGA
HHHHHHHHHEEEEECCCCCCEEEEEECCCHHHHHHHHHHHHHCCCCCCCCCHHHHHHHHH
LVHPLILTAFENGQKADELGLDFLGIPVTLLNYSSSVIPIIFSAWLCSILERRLNAWLPS
HHHHHHHHHHCCCCCHHHHCCCHHCCCHHEECCCCCHHHHHHHHHHHHHHHHHHHHHCHH
AIKNFFTPLLCLMVITPVTFLLVGPLSTWISELIAAGYLWLYQAVPAFAGAVMGGFWQIF
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
VMFGLHWGLVPLCINNFTVLGYDTMIPLLMPAIMAQVGAALGVFLCERDAQKKVVAGSAA
HHHHHHHHHHHHHHCCEEEEEHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHCCHHH
LTGLFGITEPAVYGVNLPRKYPFVIACISGALGATIIGYAQTKVYSFGLPSIFTFMQTIP
HHHHHCCCCCCEECCCCCCCCCCCEEEHHHHHHHHHHHHHHHHEEECCCHHHHHHHHHCC
STGIDFTVWASVIGGVIAIGCAFVGTVMLHFITAKRQPAQGAPQEKTPEVITPPEQGGIC
CCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCCCCCC
SPMTGEIVPLIHVADTTFASGLLGKGIAILPSVGEVRSPVAGRIASLFATLHAIGIESDD
CCCCCCCEEEEEECCHHHHHHHHCCCEEECCCCHHHHCHHHHHHHHHHHHHHHHCCCCCC
GVELLIHVGIDTVKLDGKFFSAHVNVGDKVNTGDRLISFDIPAIREAGFDLTTPVLISNS
CCEEEEEECCCEEEECCEEEEEEECCCCCCCCCCEEEEECCCHHHHCCCCCCCCEEEECC
DDFTDVLPHGTAQISAGEPLLSIIR
CCHHHHCCCCCCEECCCCHHHHHCC
>Mature Secondary Structure 
TELARKIVAGVGGADNIVSLMHCATRLRFKLKDESKAQAEVLKKTPGIIMVVESGGQFQ
HHHHHHHHHCCCCHHHHHHHHHHHHHHHEEECCCHHHHHHHHHCCCCEEEEEECCCEEE
VVIGNHVADVFLAVNSVAGLGEKAQQAPENDEKGNLLNRFVYVISGIFTPLIGLMAATGI
EEECCHHHHHHHHHHHHHCCCHHHHCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHH
LKGMLALALTFQWTTEQSGTYLILFSASDALFWFFPIILGYTAGKRFGGNPFTAMVIGGA
HHHHHHHHHEEEEECCCCCCEEEEEECCCHHHHHHHHHHHHHCCCCCCCCCHHHHHHHHH
LVHPLILTAFENGQKADELGLDFLGIPVTLLNYSSSVIPIIFSAWLCSILERRLNAWLPS
HHHHHHHHHHCCCCCHHHHCCCHHCCCHHEECCCCCHHHHHHHHHHHHHHHHHHHHHCHH
AIKNFFTPLLCLMVITPVTFLLVGPLSTWISELIAAGYLWLYQAVPAFAGAVMGGFWQIF
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
VMFGLHWGLVPLCINNFTVLGYDTMIPLLMPAIMAQVGAALGVFLCERDAQKKVVAGSAA
HHHHHHHHHHHHHHCCEEEEEHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHCCHHH
LTGLFGITEPAVYGVNLPRKYPFVIACISGALGATIIGYAQTKVYSFGLPSIFTFMQTIP
HHHHHCCCCCCEECCCCCCCCCCCEEEHHHHHHHHHHHHHHHHEEECCCHHHHHHHHHCC
STGIDFTVWASVIGGVIAIGCAFVGTVMLHFITAKRQPAQGAPQEKTPEVITPPEQGGIC
CCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCCCCCC
SPMTGEIVPLIHVADTTFASGLLGKGIAILPSVGEVRSPVAGRIASLFATLHAIGIESDD
CCCCCCCEEEEEECCHHHHHHHHCCCEEECCCCHHHHCHHHHHHHHHHHHHHHHCCCCCC
GVELLIHVGIDTVKLDGKFFSAHVNVGDKVNTGDRLISFDIPAIREAGFDLTTPVLISNS
CCEEEEEECCCEEEECCEEEEEEECCCCCCCCCCEEEEECCCHHHHCCCCCCCCEEEECC
DDFTDVLPHGTAQISAGEPLLSIIR
CCHHHHCCCCCCEECCCCHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 3309161; 3034860; 7686882; 9278503 [H]