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The map label for this gene is recF

Identifier: 218697422

GI number: 218697422

Start: 4255309

End: 4256382

Strand: Reverse

Name: recF

Synonym: EC55989_4169

Alternate gene names: 218697422

Gene position: 4256382-4255309 (Counterclockwise)

Preceding gene: 218697423

Following gene: 218697421

Centisome position: 82.57

GC content: 54.75

Gene sequence:

>1074_bases
ATGTCCCTCACCCGCTTGTTGATCCGCGATTTCCGCAACATTGAAACCGCGGATCTCGCTTTATCTCCCGGCTTTAACTT
TCTGGTAGGTGCCAACGGCAGTGGCAAAACCAGCGTGCTGGAAGCCATCTATACGCTCGGCCATGGTCGGGCGTTTCGCA
GTTTGCAGATTGGTCGCGTCATTCGCCATGAGCAGGAGGCATTTGTTCTCCATGGGCGATTACAGGGCGAAGAGCGCGAG
ACGGCGATTGGCTTAACCAAGGACAAACAGGGCGACAGCAAAGTCCGCATCGACGGTACTGACGGGCATAAAGTCGCGGA
ACTGGCGCACCTGATGCCAATGCAGCTGATAACGCCAGAAGGGTTTACTTTACTCAACGGCGGCCCCAAATACAGAAGAG
CATTCCTCGACTGGGGATGCTTTCACAACGAACCCGGATTTTTCACCGCCTGGAGCAATCTCAAGCGATTGCTCAAGCAG
CGCAATGCGGCGCTGCGCCAGGTGACACGTTACGAACAGCTACGCCCGTGGGATAAAGAACTGATCCCGCTGGCGGAGCA
AATCAGCACCTGGCGCGCGGAGTATAGCGCCGGTATCGCGGCCGATATGGCCGATACCTGTAAGCAATTTCTCCCTGAGT
TTTCTCTGACTTTCTCTTTCCAGCGCGGCTGGGAGAAAGAGACAGAATATGCTGAGGTGCTGGAACGTAATTTTGAACGC
GATCGCCAGCTAACCTACACCGCGCATGGCCCGCATAAAGCGGACTTACGCATTCGCGCCGACGGTGCGCCGGTGGAAGA
TACCTTATCGCGTGGGCAGCTTAAGCTGTTGATGTGCGCCTTACGTCTGGCGCAAGGAGAGTTCCTCACCCGTGAAAGCG
GGCGGCGGTGTCTCTACCTGATAGATGATTTTGCCTCTGAGCTTGATGATGAGCGTCGCGGGCTGCTTGCCAGCCGCTTA
AAAGCGACGCAATCACAGGTCTTTGTCAGCGCGATCAGTGCTGAACACGTTATAGACATGTCGGACGAAAATTCGAAGAT
GTTTACCGTGGAAAAGGGTAAAATAACGGATTAA

Upstream 100 bases:

>100_bases
AAAACGTCCGCATGATGCTGACCGATTCGGTTTCCAGCGTGCAGATTGAAGATGCCGCATCACAGTCGGCTGCCTATGTT
GTCATGCCAATGAGACTGTA

Downstream 100 bases:

>100_bases
CCCAAGTATAAATGAGCGAGAAACGTTGATGTCGAATTCTTATGACTCCTCCAGTATCAAAGTCCTGAAAGGGCTGGATG
CGGTGCGTAAGCGCCCGGGT

Product: recombination protein F

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 357; Mature: 356

Protein sequence:

>357_residues
MSLTRLLIRDFRNIETADLALSPGFNFLVGANGSGKTSVLEAIYTLGHGRAFRSLQIGRVIRHEQEAFVLHGRLQGEERE
TAIGLTKDKQGDSKVRIDGTDGHKVAELAHLMPMQLITPEGFTLLNGGPKYRRAFLDWGCFHNEPGFFTAWSNLKRLLKQ
RNAALRQVTRYEQLRPWDKELIPLAEQISTWRAEYSAGIAADMADTCKQFLPEFSLTFSFQRGWEKETEYAEVLERNFER
DRQLTYTAHGPHKADLRIRADGAPVEDTLSRGQLKLLMCALRLAQGEFLTRESGRRCLYLIDDFASELDDERRGLLASRL
KATQSQVFVSAISAEHVIDMSDENSKMFTVEKGKITD

Sequences:

>Translated_357_residues
MSLTRLLIRDFRNIETADLALSPGFNFLVGANGSGKTSVLEAIYTLGHGRAFRSLQIGRVIRHEQEAFVLHGRLQGEERE
TAIGLTKDKQGDSKVRIDGTDGHKVAELAHLMPMQLITPEGFTLLNGGPKYRRAFLDWGCFHNEPGFFTAWSNLKRLLKQ
RNAALRQVTRYEQLRPWDKELIPLAEQISTWRAEYSAGIAADMADTCKQFLPEFSLTFSFQRGWEKETEYAEVLERNFER
DRQLTYTAHGPHKADLRIRADGAPVEDTLSRGQLKLLMCALRLAQGEFLTRESGRRCLYLIDDFASELDDERRGLLASRL
KATQSQVFVSAISAEHVIDMSDENSKMFTVEKGKITD
>Mature_356_residues
SLTRLLIRDFRNIETADLALSPGFNFLVGANGSGKTSVLEAIYTLGHGRAFRSLQIGRVIRHEQEAFVLHGRLQGEERET
AIGLTKDKQGDSKVRIDGTDGHKVAELAHLMPMQLITPEGFTLLNGGPKYRRAFLDWGCFHNEPGFFTAWSNLKRLLKQR
NAALRQVTRYEQLRPWDKELIPLAEQISTWRAEYSAGIAADMADTCKQFLPEFSLTFSFQRGWEKETEYAEVLERNFERD
RQLTYTAHGPHKADLRIRADGAPVEDTLSRGQLKLLMCALRLAQGEFLTRESGRRCLYLIDDFASELDDERRGLLASRLK
ATQSQVFVSAISAEHVIDMSDENSKMFTVEKGKITD

Specific function: The recF protein is involved in DNA metabolism; it is required for DNA replication and normal SOS inducibility. RecF binds preferentially to single-stranded, linear DNA. It also seems to bind ATP [H]

COG id: COG1195

COG function: function code L; Recombinational DNA repair ATPase (RecF pathway)

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the recF family [H]

Homologues:

Organism=Escherichia coli, GI1790135, Length=357, Percent_Identity=100, Blast_Score=736, Evalue=0.0,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001238
- InterPro:   IPR018078
- InterPro:   IPR003395 [H]

Pfam domain/function: PF02463 SMC_N [H]

EC number: NA

Molecular weight: Translated: 40515; Mature: 40383

Theoretical pI: Translated: 7.29; Mature: 7.29

Prosite motif: PS00617 RECF_1 ; PS00618 RECF_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.1 %Cys     (Translated Protein)
2.0 %Met     (Translated Protein)
3.1 %Cys+Met (Translated Protein)
1.1 %Cys     (Mature Protein)
1.7 %Met     (Mature Protein)
2.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSLTRLLIRDFRNIETADLALSPGFNFLVGANGSGKTSVLEAIYTLGHGRAFRSLQIGRV
CCHHHHHHHHHCCCCHHHEEECCCCCEEEECCCCCHHHHHHHHHHHCCCHHHHHHHHHHH
IRHEQEAFVLHGRLQGEERETAIGLTKDKQGDSKVRIDGTDGHKVAELAHLMPMQLITPE
HHHCCCEEEEEEECCCCCHHHEECCCCCCCCCCEEEEECCCCHHHHHHHHHHHHHEECCC
GFTLLNGGPKYRRAFLDWGCFHNEPGFFTAWSNLKRLLKQRNAALRQVTRYEQLRPWDKE
CCEEECCCHHHHHHHHHCCCCCCCCCCEEHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHH
LIPLAEQISTWRAEYSAGIAADMADTCKQFLPEFSLTFSFQRGWEKETEYAEVLERNFER
HHHHHHHHHHHHHHHHCCCHHHHHHHHHHHCCCCEEEEEHHCCCCCCHHHHHHHHHHHHH
DRQLTYTAHGPHKADLRIRADGAPVEDTLSRGQLKLLMCALRLAQGEFLTRESGRRCLYL
CCEEEEEECCCCCCCEEEEECCCCHHHHHCCCHHHHHHHHHHHHCCCCEECCCCCEEEEE
IDDFASELDDERRGLLASRLKATQSQVFVSAISAEHVIDMSDENSKMFTVEKGKITD
HHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHEEECCCCCCEEEEEECCCCCC
>Mature Secondary Structure 
SLTRLLIRDFRNIETADLALSPGFNFLVGANGSGKTSVLEAIYTLGHGRAFRSLQIGRV
CHHHHHHHHHCCCCHHHEEECCCCCEEEECCCCCHHHHHHHHHHHCCCHHHHHHHHHHH
IRHEQEAFVLHGRLQGEERETAIGLTKDKQGDSKVRIDGTDGHKVAELAHLMPMQLITPE
HHHCCCEEEEEEECCCCCHHHEECCCCCCCCCCEEEEECCCCHHHHHHHHHHHHHEECCC
GFTLLNGGPKYRRAFLDWGCFHNEPGFFTAWSNLKRLLKQRNAALRQVTRYEQLRPWDKE
CCEEECCCHHHHHHHHHCCCCCCCCCCEEHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHH
LIPLAEQISTWRAEYSAGIAADMADTCKQFLPEFSLTFSFQRGWEKETEYAEVLERNFER
HHHHHHHHHHHHHHHHCCCHHHHHHHHHHHCCCCEEEEEHHCCCCCCHHHHHHHHHHHHH
DRQLTYTAHGPHKADLRIRADGAPVEDTLSRGQLKLLMCALRLAQGEFLTRESGRRCLYL
CCEEEEEECCCCCCCEEEEECCCCHHHHHCCCHHHHHHHHHHHHCCCCEECCCCCEEEEE
IDDFASELDDERRGLLASRLKATQSQVFVSAISAEHVIDMSDENSKMFTVEKGKITD
HHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHEEECCCCCCEEEEEECCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA