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The map label for this gene is nepI

Identifier: 218697384

GI number: 218697384

Start: 4215571

End: 4216926

Strand: Reverse

Name: nepI

Synonym: EC55989_4130

Alternate gene names: 218697384

Gene position: 4216926-4215571 (Counterclockwise)

Preceding gene: 218697385

Following gene: 218697382

Centisome position: 81.8

GC content: 52.21

Gene sequence:

>1356_bases
GTGGATAAGACGTTCACGCCGCATCCGGTAATCTTTGCCCTGTTCCGTTTCGATCTTAACTCCAGCTCAAGCAACACCGT
AAAAGTTGCAAAAACGTTAAATGCGTCACACATTTCAATGTTAAAGTGGTCGGCTTTTCCCCTGAAACATGCCACGGGTA
ACACCATGAGTGAATTTATTGCCGAAAACCGCGGCGCGGATGCCATCACCCGACCGAACTGGTCAGCCGTTTTCTCGGTG
GCGTTTTGTGTCGCCTGTCTGATTATCGTTGAGTTTTTGCCCGTCAGTTTGTTGACGCCAATGGCCCAGGATTTAGGCAT
TTCGGAAGGGGTTGCCGGGCAATCGGTGACCGTGACCGCCTTTGTGGCAATGTTTGCCAGTTTGTTTATTACCCAGACAA
TTCAGGCTACTGACCGCCGCTACGTTGTTATTTTGTTTGCCGTTTTGCTGACGCTCTCCTGCTTGCTGGTTTCCTTTGCT
AACTCATTCAGTTTGCTTTTAATCGGTCGTGCCTGTCTGGGGCTGGCGCTGGGCGGGTTCTGGGCGATGTCGGCGTCGCT
GACCATGCGTCTGGTGCCGCCGCGTACGGTGCCGAAGGCGCTGTCGGTGATCTTCGGCGCGGTTTCTATTGCGCTGGTGA
TTGCCGCGCCGTTGGGCAGTTTTTTAGGCGAGCTTATCGGTTGGCGCAATGTCTTTAATGCGGCGGCGGTGATGGGCGTG
CTGTGTATTTTCTGGATTATCAAATCATTGCCTTCACTGCCAGGCGAACCCTCGCATCAGAAACAAAATACTTTCCGCTT
ATTACAACGTCCGGGTGTGATGGCAGGGATGATCGCCATCTTCATGTCTTTCGCCGGGCAGTTTGCTTTCTTCACATATA
TTCGCCCGGTGTATATGAACCTGGCGGGATTCGGCGTGGATGGCTTAACGCTGGTGCTGTTGAGTTTTGGCATTGCCAGC
TTTATCGGTACGTCGCTTTCGTCATTCATTCTTAAACGTTCGGTAAAACTGGCCTTAGCAGGCGCGCCGTTAATACTGGC
TGTGAGTGCGTTGGTGCTGACGTTGTGGGGAAGCGATAAAATCGTTGCTACCGGCGTGGCGATTATCTGGGGGCTAACTT
TTGCATTGGTTCCCGTCGGCTGGTCAACGTGGATCACCCGCTCGCTGGCCGATCAGGCAGAAAAAGCCGGGTCTATTCAG
GTGGCGGTTATTCAGCTTGCTAATACCTGTGGCGCGGCAATCGGCGGTTATGCGCTGGATAATATTGGTCTGACTTCGCC
GTTGATGTTGTCCGGCACATTGATGTTGCTGACTGCATTGTTGGTTACTGCAAAGGTGAAAATGAAGAAATCCTGA

Upstream 100 bases:

>100_bases
GATGCGCTATAACGCGTTCTTTCAGCCTGCCGAGGGCGTTCACTGGTTGCATTGAAACCGTTTCGTCTTATCAGCCTACA
GACCGCGCCTGACCTGTCGG

Downstream 100 bases:

>100_bases
TATCAGGTAAAGAAGCGTTGGTCAGTAGTTGTTGCTGTTCTTACATATCCGGGCATTCTACCTTACCCAACGCTTCCCAA
TAATTGCTCTGATTATTACG

Product: ribonucleoside transporter

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 451; Mature: 451

Protein sequence:

>451_residues
MDKTFTPHPVIFALFRFDLNSSSSNTVKVAKTLNASHISMLKWSAFPLKHATGNTMSEFIAENRGADAITRPNWSAVFSV
AFCVACLIIVEFLPVSLLTPMAQDLGISEGVAGQSVTVTAFVAMFASLFITQTIQATDRRYVVILFAVLLTLSCLLVSFA
NSFSLLLIGRACLGLALGGFWAMSASLTMRLVPPRTVPKALSVIFGAVSIALVIAAPLGSFLGELIGWRNVFNAAAVMGV
LCIFWIIKSLPSLPGEPSHQKQNTFRLLQRPGVMAGMIAIFMSFAGQFAFFTYIRPVYMNLAGFGVDGLTLVLLSFGIAS
FIGTSLSSFILKRSVKLALAGAPLILAVSALVLTLWGSDKIVATGVAIIWGLTFALVPVGWSTWITRSLADQAEKAGSIQ
VAVIQLANTCGAAIGGYALDNIGLTSPLMLSGTLMLLTALLVTAKVKMKKS

Sequences:

>Translated_451_residues
MDKTFTPHPVIFALFRFDLNSSSSNTVKVAKTLNASHISMLKWSAFPLKHATGNTMSEFIAENRGADAITRPNWSAVFSV
AFCVACLIIVEFLPVSLLTPMAQDLGISEGVAGQSVTVTAFVAMFASLFITQTIQATDRRYVVILFAVLLTLSCLLVSFA
NSFSLLLIGRACLGLALGGFWAMSASLTMRLVPPRTVPKALSVIFGAVSIALVIAAPLGSFLGELIGWRNVFNAAAVMGV
LCIFWIIKSLPSLPGEPSHQKQNTFRLLQRPGVMAGMIAIFMSFAGQFAFFTYIRPVYMNLAGFGVDGLTLVLLSFGIAS
FIGTSLSSFILKRSVKLALAGAPLILAVSALVLTLWGSDKIVATGVAIIWGLTFALVPVGWSTWITRSLADQAEKAGSIQ
VAVIQLANTCGAAIGGYALDNIGLTSPLMLSGTLMLLTALLVTAKVKMKKS
>Mature_451_residues
MDKTFTPHPVIFALFRFDLNSSSSNTVKVAKTLNASHISMLKWSAFPLKHATGNTMSEFIAENRGADAITRPNWSAVFSV
AFCVACLIIVEFLPVSLLTPMAQDLGISEGVAGQSVTVTAFVAMFASLFITQTIQATDRRYVVILFAVLLTLSCLLVSFA
NSFSLLLIGRACLGLALGGFWAMSASLTMRLVPPRTVPKALSVIFGAVSIALVIAAPLGSFLGELIGWRNVFNAAAVMGV
LCIFWIIKSLPSLPGEPSHQKQNTFRLLQRPGVMAGMIAIFMSFAGQFAFFTYIRPVYMNLAGFGVDGLTLVLLSFGIAS
FIGTSLSSFILKRSVKLALAGAPLILAVSALVLTLWGSDKIVATGVAIIWGLTFALVPVGWSTWITRSLADQAEKAGSIQ
VAVIQLANTCGAAIGGYALDNIGLTSPLMLSGTLMLLTALLVTAKVKMKKS

Specific function: Involved in the efflux of purine ribonucleosides, such as guanosine, adenosine and especially inosine. Involved in the resistance to 6-mercaptopurine [H]

COG id: COG2814

COG function: function code G; Arabinose efflux permease

Gene ontology:

Cell location: Cell inner membrane; Multi-pass membrane protein [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the major facilitator superfamily. DHA1 family. NepI (TC 2.A.1.2.26) subfamily [H]

Homologues:

Organism=Escherichia coli, GI145693198, Length=396, Percent_Identity=100, Blast_Score=778, Evalue=0.0,
Organism=Escherichia coli, GI1787808, Length=235, Percent_Identity=31.063829787234, Blast_Score=101, Evalue=8e-23,
Organism=Escherichia coli, GI1787947, Length=289, Percent_Identity=26.9896193771626, Blast_Score=97, Evalue=3e-21,
Organism=Escherichia coli, GI1786595, Length=348, Percent_Identity=25, Blast_Score=89, Evalue=7e-19,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR020846
- InterPro:   IPR011701
- InterPro:   IPR016196 [H]

Pfam domain/function: PF07690 MFS_1 [H]

EC number: NA

Molecular weight: Translated: 47932; Mature: 47932

Theoretical pI: Translated: 10.38; Mature: 10.38

Prosite motif: PS50850 MFS

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.3 %Cys     (Translated Protein)
3.3 %Met     (Translated Protein)
4.7 %Cys+Met (Translated Protein)
1.3 %Cys     (Mature Protein)
3.3 %Met     (Mature Protein)
4.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure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CCCCCCHHHHHHHHHHHHHHHHHHHHHHCCC
>Mature Secondary Structure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CCCCCCHHHHHHHHHHHHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: NA