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The map label for this gene is ligB

Identifier: 218697368

GI number: 218697368

Start: 4194513

End: 4196195

Strand: Reverse

Name: ligB

Synonym: EC55989_4112

Alternate gene names: 218697368

Gene position: 4196195-4194513 (Counterclockwise)

Preceding gene: 218697374

Following gene: 218697364

Centisome position: 81.4

GC content: 52.88

Gene sequence:

>1683_bases
ATGAAAGTATGGATGGCGATATTAATAAGTATCTTGTGCTGGCAATCATCTGTGTGGGCGGTCTGTCCGGCCTGGTCGCC
AGCCAGAGCACAGGAAGAAATTTCCCGCCTGCAACAGCAAATAAAACAGTGGGACGATGACTACTGGAAGGAAGGAAAAA
GTGAGGTGGAAGACGGTGTTTACGATCAGTTAAGCGCCCGTCTTACGCAGTGGCAACGCTGCTTTGGGAGCGAGCCCCGC
GACGTGATGATGCCGCCTTTAAATGGTGCGGTTATGCATCCGGTTGCCCATACGGGTGTACGCAAAATGGTGGATAAAAA
CGCATTAAGTCTGTGGATGCGAGAACGTAGCGATCTTTGGGTGCAGCCAAAAGTTGATGGCGTTGCGGTAACCCTGGTTT
ATCGGGACGGGAAACTGAACAAAGCAATCAGTCGCGGTAATGGCCTGAAAGGCGAGGACTGGACGCAGAAAGTTAGCTTA
ATTTCCGCTGTGCCGCAAACCGTTAGCGGGCCTTTAGCCAACAGTACGCTTCAGGGGGAAATATTTCTCCAGCGCGAGGG
GCATATCCAACAACAAATGGGGGGAATAAATGCCCGCGCAAAAGTTGCTGGCCTGATGATGCGCCAGGACGATAGCGACA
CGCTGAATTCTCTGGGCGTTTTTGTCTGGGCATGGCCGGATGGACCGCAGTTAATGACCGATCGTTTAAAGGAGCTGGCT
ACCGCAGGTTTTACTCTAACGCAGAGGTATACCCGTGCGGTTAAAAATGCTGATGAGGTTGCGCGCGTACGCAATGAGTG
GTGGAAAGCGAAATTACCCTTCGTCACCGATGGCGTGGTTGTGCGAGGGGCGAAAGAACCCGAATCCCGCCATTGGCTAC
CAGGCCAGGCAGAATGGCTGGTGGCCTGGAAATATCAACCTGTAGCTCAGGTTGCCGAAGTGAAGGCGATTCAGTTTGCG
GTGGGTAAGAGCGGTAAAATATCGGTGGTTGCGTCACTCGCACCTGTCATGCTGGATGATAAAAAAGTCCAGCGGGTGAA
TATTGGTTCCGTCAGGCGCTGGCAGGAGTGGGATATTGCGCCTGGTGATCAGATCCTCGTCAGCCTTGCCGGTCAGGGTA
TTCCTCGCATTGATGATGTGGTCTGGCGCGGTGCAGAACGTACAAAACCGACACCACCAGAAAACCGCTTTAACTCGTTG
ACCTGCTACTTTGCTTCTGATGTTTGTCAGGAACAGTTCATTTCACGCTTAGTCTGGCTGGGGTCAAAACAGGTTCTGGG
GCTGGATGGCATTGGTGAGGCCGGCTGGCGCGCGCTGCATCAGACTCATCGCTTTGAGCATATCTTTTCCTGGCTTTTAT
TAACGCCAGAGCAATTACAGAACACGCCGGGGATCGCGAAAAGTAAAAGTGCGCAGCTATGGCATCAGTTTAATCTGGCT
CGTAAGCAGCCTTTTACTCGCTGGGTGATGGCAATGGGAATACCGCTAACCCGGGCGGCGCTTAATGCCAGTGATGAACG
GTCCTGGTCGCAACTTTTATTTAGCACGGAGCAGTTCTGGCAGCAGCTGCCGGGGACTGGATCGGGGCGCGCCAGACAGG
TTATTGAATGGAAGGAAAATGCGCAAATCAAGAAGCTGGGCAGTTGGCTGGCTGCCCAGCAGATCACAGGTTTTGAACCT
TAG

Upstream 100 bases:

>100_bases
GACGATTCTCAGGAAGCGTCCTCGCAGACTCCGTTTTTGCCACTCGGCTTTGCCTTCACCACGTTGTATATCTGCCAGCC
TGACTACAGCGGAGGTGAAG

Downstream 100 bases:

>100_bases
TGTCCTTCGTGGCTGTAGTAAAAGACCGGTAATCCCAGCTTAAGGCGTAGCGCCAGTAAGCGGGCAAAAAAGCCGAATAC
CAGGGTGGAGATAATTACCA

Product: NAD-dependent DNA ligase LigB

Products: NA

Alternate protein names: Polydeoxyribonucleotide synthase [NAD+] B [H]

Number of amino acids: Translated: 560; Mature: 560

Protein sequence:

>560_residues
MKVWMAILISILCWQSSVWAVCPAWSPARAQEEISRLQQQIKQWDDDYWKEGKSEVEDGVYDQLSARLTQWQRCFGSEPR
DVMMPPLNGAVMHPVAHTGVRKMVDKNALSLWMRERSDLWVQPKVDGVAVTLVYRDGKLNKAISRGNGLKGEDWTQKVSL
ISAVPQTVSGPLANSTLQGEIFLQREGHIQQQMGGINARAKVAGLMMRQDDSDTLNSLGVFVWAWPDGPQLMTDRLKELA
TAGFTLTQRYTRAVKNADEVARVRNEWWKAKLPFVTDGVVVRGAKEPESRHWLPGQAEWLVAWKYQPVAQVAEVKAIQFA
VGKSGKISVVASLAPVMLDDKKVQRVNIGSVRRWQEWDIAPGDQILVSLAGQGIPRIDDVVWRGAERTKPTPPENRFNSL
TCYFASDVCQEQFISRLVWLGSKQVLGLDGIGEAGWRALHQTHRFEHIFSWLLLTPEQLQNTPGIAKSKSAQLWHQFNLA
RKQPFTRWVMAMGIPLTRAALNASDERSWSQLLFSTEQFWQQLPGTGSGRARQVIEWKENAQIKKLGSWLAAQQITGFEP

Sequences:

>Translated_560_residues
MKVWMAILISILCWQSSVWAVCPAWSPARAQEEISRLQQQIKQWDDDYWKEGKSEVEDGVYDQLSARLTQWQRCFGSEPR
DVMMPPLNGAVMHPVAHTGVRKMVDKNALSLWMRERSDLWVQPKVDGVAVTLVYRDGKLNKAISRGNGLKGEDWTQKVSL
ISAVPQTVSGPLANSTLQGEIFLQREGHIQQQMGGINARAKVAGLMMRQDDSDTLNSLGVFVWAWPDGPQLMTDRLKELA
TAGFTLTQRYTRAVKNADEVARVRNEWWKAKLPFVTDGVVVRGAKEPESRHWLPGQAEWLVAWKYQPVAQVAEVKAIQFA
VGKSGKISVVASLAPVMLDDKKVQRVNIGSVRRWQEWDIAPGDQILVSLAGQGIPRIDDVVWRGAERTKPTPPENRFNSL
TCYFASDVCQEQFISRLVWLGSKQVLGLDGIGEAGWRALHQTHRFEHIFSWLLLTPEQLQNTPGIAKSKSAQLWHQFNLA
RKQPFTRWVMAMGIPLTRAALNASDERSWSQLLFSTEQFWQQLPGTGSGRARQVIEWKENAQIKKLGSWLAAQQITGFEP
>Mature_560_residues
MKVWMAILISILCWQSSVWAVCPAWSPARAQEEISRLQQQIKQWDDDYWKEGKSEVEDGVYDQLSARLTQWQRCFGSEPR
DVMMPPLNGAVMHPVAHTGVRKMVDKNALSLWMRERSDLWVQPKVDGVAVTLVYRDGKLNKAISRGNGLKGEDWTQKVSL
ISAVPQTVSGPLANSTLQGEIFLQREGHIQQQMGGINARAKVAGLMMRQDDSDTLNSLGVFVWAWPDGPQLMTDRLKELA
TAGFTLTQRYTRAVKNADEVARVRNEWWKAKLPFVTDGVVVRGAKEPESRHWLPGQAEWLVAWKYQPVAQVAEVKAIQFA
VGKSGKISVVASLAPVMLDDKKVQRVNIGSVRRWQEWDIAPGDQILVSLAGQGIPRIDDVVWRGAERTKPTPPENRFNSL
TCYFASDVCQEQFISRLVWLGSKQVLGLDGIGEAGWRALHQTHRFEHIFSWLLLTPEQLQNTPGIAKSKSAQLWHQFNLA
RKQPFTRWVMAMGIPLTRAALNASDERSWSQLLFSTEQFWQQLPGTGSGRARQVIEWKENAQIKKLGSWLAAQQITGFEP

Specific function: Catalyzes the formation of phosphodiester linkages between 5'-phosphoryl and 3'-hydroxyl groups in double-stranded DNA using NAD as a coenzyme and as the energy source for the reaction [H]

COG id: COG0272

COG function: function code L; NAD-dependent DNA ligase (contains BRCT domain type II)

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the NAD-dependent DNA ligase family. LigB subfamily [H]

Homologues:

Organism=Escherichia coli, GI87082305, Length=560, Percent_Identity=98.9285714285714, Blast_Score=1131, Evalue=0.0,
Organism=Escherichia coli, GI1788750, Length=518, Percent_Identity=23.7451737451737, Blast_Score=106, Evalue=4e-24,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR018239
- InterPro:   IPR020923
- InterPro:   IPR004150
- InterPro:   IPR013839
- InterPro:   IPR013840
- InterPro:   IPR012340
- InterPro:   IPR016027
- InterPro:   IPR010994 [H]

Pfam domain/function: PF01653 DNA_ligase_aden; PF03120 DNA_ligase_OB [H]

EC number: =6.5.1.2 [H]

Molecular weight: Translated: 63280; Mature: 63280

Theoretical pI: Translated: 9.84; Mature: 9.84

Prosite motif: PS01055 DNA_LIGASE_N1 ; PS01056 DNA_LIGASE_N2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.9 %Cys     (Translated Protein)
2.5 %Met     (Translated Protein)
3.4 %Cys+Met (Translated Protein)
0.9 %Cys     (Mature Protein)
2.5 %Met     (Mature Protein)
3.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKVWMAILISILCWQSSVWAVCPAWSPARAQEEISRLQQQIKQWDDDYWKEGKSEVEDGV
CHHHHHHHHHHHHHCCCCEEEECCCCCHHHHHHHHHHHHHHHHCCHHHHHCCHHHHHHHH
YDQLSARLTQWQRCFGSEPRDVMMPPLNGAVMHPVAHTGVRKMVDKNALSLWMRERSDLW
HHHHHHHHHHHHHHHCCCCCCEECCCCCCCEECHHHHHHHHHHHHHHHHHHHHHHCCCEE
VQPKVDGVAVTLVYRDGKLNKAISRGNGLKGEDWTQKVSLISAVPQTVSGPLANSTLQGE
ECCCCCCEEEEEEEECCCHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHCCCCCCCCCCCE
IFLQREGHIQQQMGGINARAKVAGLMMRQDDSDTLNSLGVFVWAWPDGPQLMTDRLKELA
EEEECCCCHHHHHCCCCHHHHHHHHHHCCCCCHHHHCCCEEEEECCCCHHHHHHHHHHHH
TAGFTLTQRYTRAVKNADEVARVRNEWWKAKLPFVTDGVVVRGAKEPESRHWLPGQAEWL
HCCCHHHHHHHHHHHCHHHHHHHHHHHHCCCCCEEECCEEEECCCCCCCCCCCCCCCCEE
VAWKYQPVAQVAEVKAIQFAVGKSGKISVVASLAPVMLDDKKVQRVNIGSVRRWQEWDIA
EEEECCHHHHHHHHHHHHHCCCCCCCEEEHHHHHHHHCCCCHHEEECCCCCCCCCCCCCC
PGDQILVSLAGQGIPRIDDVVWRGAERTKPTPPENRFNSLTCYFASDVCQEQFISRLVWL
CCHHHHHHHHCCCCCCHHHHHHCCCCCCCCCCCCCCCCCEEEEHHHHHHHHHHHHHHHHC
GSKQVLGLDGIGEAGWRALHQTHRFEHIFSWLLLTPEQLQNTPGIAKSKSAQLWHQFNLA
CCCCEECCCCCCHHHHHHHHHHHHHHHHHHHHHCCHHHHCCCCCCCCCCHHHHHHHHHHH
RKQPFTRWVMAMGIPLTRAALNASDERSWSQLLFSTEQFWQQLPGTGSGRARQVIEWKEN
HCCHHHHHHHHHCCCHHHHHHCCCCHHHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHCC
AQIKKLGSWLAAQQITGFEP
CHHHHHHHHHHHHHHCCCCC
>Mature Secondary Structure
MKVWMAILISILCWQSSVWAVCPAWSPARAQEEISRLQQQIKQWDDDYWKEGKSEVEDGV
CHHHHHHHHHHHHHCCCCEEEECCCCCHHHHHHHHHHHHHHHHCCHHHHHCCHHHHHHHH
YDQLSARLTQWQRCFGSEPRDVMMPPLNGAVMHPVAHTGVRKMVDKNALSLWMRERSDLW
HHHHHHHHHHHHHHHCCCCCCEECCCCCCCEECHHHHHHHHHHHHHHHHHHHHHHCCCEE
VQPKVDGVAVTLVYRDGKLNKAISRGNGLKGEDWTQKVSLISAVPQTVSGPLANSTLQGE
ECCCCCCEEEEEEEECCCHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHCCCCCCCCCCCE
IFLQREGHIQQQMGGINARAKVAGLMMRQDDSDTLNSLGVFVWAWPDGPQLMTDRLKELA
EEEECCCCHHHHHCCCCHHHHHHHHHHCCCCCHHHHCCCEEEEECCCCHHHHHHHHHHHH
TAGFTLTQRYTRAVKNADEVARVRNEWWKAKLPFVTDGVVVRGAKEPESRHWLPGQAEWL
HCCCHHHHHHHHHHHCHHHHHHHHHHHHCCCCCEEECCEEEECCCCCCCCCCCCCCCCEE
VAWKYQPVAQVAEVKAIQFAVGKSGKISVVASLAPVMLDDKKVQRVNIGSVRRWQEWDIA
EEEECCHHHHHHHHHHHHHCCCCCCCEEEHHHHHHHHCCCCHHEEECCCCCCCCCCCCCC
PGDQILVSLAGQGIPRIDDVVWRGAERTKPTPPENRFNSLTCYFASDVCQEQFISRLVWL
CCHHHHHHHHCCCCCCHHHHHHCCCCCCCCCCCCCCCCCEEEEHHHHHHHHHHHHHHHHC
GSKQVLGLDGIGEAGWRALHQTHRFEHIFSWLLLTPEQLQNTPGIAKSKSAQLWHQFNLA
CCCCEECCCCCCHHHHHHHHHHHHHHHHHHHHHCCHHHHCCCCCCCCCCHHHHHHHHHHH
RKQPFTRWVMAMGIPLTRAALNASDERSWSQLLFSTEQFWQQLPGTGSGRARQVIEWKEN
HCCHHHHHHHHHCCCHHHHHHCCCCHHHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHCC
AQIKKLGSWLAAQQITGFEP
CHHHHHHHHHHHHHHCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA