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The map label for this gene is rfaY

Identifier: 218697349

GI number: 218697349

Start: 4178397

End: 4179104

Strand: Reverse

Name: rfaY

Synonym: EC55989_4092

Alternate gene names: 218697349

Gene position: 4179104-4178397 (Counterclockwise)

Preceding gene: 218697350

Following gene: 218697348

Centisome position: 81.07

GC content: 30.51

Gene sequence:

>708_bases
ATGATATATAATAAAACGATAAATGGGTTGAAAGTTTTCATAAAAGATAATGATCCCTTTTATGAGCAAGTTCTAAATGA
CTTTTTAACTTGCAGGGTTAAAACATTAAAAGTTTTTCGTTCGATTGATGATACTAAAGTCATTCTTATTGACACTGCTC
GTGGACCTCTTGTACTTAAAGTTTATGCGCCAAAGCATAAAATGACCGAACGTTTCTTAAAATCTTGCATAAAAAAAGAT
TATTATGAAAATTTAATTTATCAAACCGATCGGGTACGTGGTGAAGGCATCCAATCAATTAATGACTATTTTTTGCTAGC
TGAACGTAAAACATTAAATTTCGCTCATTATTATATTATGCTCATTGAGTATATTGAAGGTGTCGGGTTAAATGAGTATT
TGGAAATTTCTGAAGATCTGAAGGATCAATTATCAGAATCGATAAAAGAGTTGCATCAGCATGGAATGGTGTCTGGCGAT
CCTCATAAAGGAAATTTTATAGTATCCGAAAAAGGTTTAAGACTAATTGACCTTTCAGGTAAAAAAACAACCGCTGTTTT
AAAAGCAAAAGATCGCATTGATCTTGAACGGCACTATAATATAAAAAATGAATTGAAAGACTTTGGCTATACATATCTTA
TATTCAAAAAGAAAATTAAAAAAGTAATCAGGGATGTAAAAGTCAAACTAGGATTAAAGAGCAAATAA

Upstream 100 bases:

>100_bases
CATAAATTTAAACAAAAGCATTATATGGCAGGCATTTTTAATTATTTAAAGTATTATAAAGAAAAATGCTTCTAAAGATT
TAAGGTTAAAATAATTCATT

Downstream 100 bases:

>100_bases
TGATTATGATGAAGGGTAATTAAATTGGATTTTAAACATCTTACTCAATTTAAAGATATAATTGAACTGGACAAGCGCCC
CGTTAAACTTGATGAACGGG

Product: lipopolysaccharide core biosynthesis protein

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 235; Mature: 235

Protein sequence:

>235_residues
MIYNKTINGLKVFIKDNDPFYEQVLNDFLTCRVKTLKVFRSIDDTKVILIDTARGPLVLKVYAPKHKMTERFLKSCIKKD
YYENLIYQTDRVRGEGIQSINDYFLLAERKTLNFAHYYIMLIEYIEGVGLNEYLEISEDLKDQLSESIKELHQHGMVSGD
PHKGNFIVSEKGLRLIDLSGKKTTAVLKAKDRIDLERHYNIKNELKDFGYTYLIFKKKIKKVIRDVKVKLGLKSK

Sequences:

>Translated_235_residues
MIYNKTINGLKVFIKDNDPFYEQVLNDFLTCRVKTLKVFRSIDDTKVILIDTARGPLVLKVYAPKHKMTERFLKSCIKKD
YYENLIYQTDRVRGEGIQSINDYFLLAERKTLNFAHYYIMLIEYIEGVGLNEYLEISEDLKDQLSESIKELHQHGMVSGD
PHKGNFIVSEKGLRLIDLSGKKTTAVLKAKDRIDLERHYNIKNELKDFGYTYLIFKKKIKKVIRDVKVKLGLKSK
>Mature_235_residues
MIYNKTINGLKVFIKDNDPFYEQVLNDFLTCRVKTLKVFRSIDDTKVILIDTARGPLVLKVYAPKHKMTERFLKSCIKKD
YYENLIYQTDRVRGEGIQSINDYFLLAERKTLNFAHYYIMLIEYIEGVGLNEYLEISEDLKDQLSESIKELHQHGMVSGD
PHKGNFIVSEKGLRLIDLSGKKTTAVLKAKDRIDLERHYNIKNELKDFGYTYLIFKKKIKKVIRDVKVKLGLKSK

Specific function: Catalyzes the phosphorylation of heptose(II) of the outer membrane lipopolysaccharide core [H]

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the protein kinase superfamily. RfaY/waaY family [H]

Homologues:

Organism=Escherichia coli, GI1790055, Length=229, Percent_Identity=56.3318777292576, Blast_Score=262, Evalue=1e-71,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR011009
- InterPro:   IPR009330 [H]

Pfam domain/function: PF06176 WaaY [H]

EC number: 2.7.-.-

Molecular weight: Translated: 27509; Mature: 27509

Theoretical pI: Translated: 9.84; Mature: 9.84

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.9 %Cys     (Translated Protein)
1.7 %Met     (Translated Protein)
2.6 %Cys+Met (Translated Protein)
0.9 %Cys     (Mature Protein)
1.7 %Met     (Mature Protein)
2.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MIYNKTINGLKVFIKDNDPFYEQVLNDFLTCRVKTLKVFRSIDDTKVILIDTARGPLVLK
CCCCCCCCCEEEEEECCCCHHHHHHHHHHHHHHHHHHHHHCCCCCEEEEEECCCCCEEEE
VYAPKHKMTERFLKSCIKKDYYENLIYQTDRVRGEGIQSINDYFLLAERKTLNFAHYYIM
EECCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCHHHCCEEEEEECCHHHHHHHHHH
LIEYIEGVGLNEYLEISEDLKDQLSESIKELHQHGMVSGDPHKGNFIVSEKGLRLIDLSG
HHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCEEEECCCEEEEEECC
KKTTAVLKAKDRIDLERHYNIKNELKDFGYTYLIFKKKIKKVIRDVKVKLGLKSK
CCEEEEEECCCCCCHHHHCCHHHHHHHCCCCHHHHHHHHHHHHHHHHHHCCCCCC
>Mature Secondary Structure
MIYNKTINGLKVFIKDNDPFYEQVLNDFLTCRVKTLKVFRSIDDTKVILIDTARGPLVLK
CCCCCCCCCEEEEEECCCCHHHHHHHHHHHHHHHHHHHHHCCCCCEEEEEECCCCCEEEE
VYAPKHKMTERFLKSCIKKDYYENLIYQTDRVRGEGIQSINDYFLLAERKTLNFAHYYIM
EECCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCHHHCCEEEEEECCHHHHHHHHHH
LIEYIEGVGLNEYLEISEDLKDQLSESIKELHQHGMVSGDPHKGNFIVSEKGLRLIDLSG
HHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCEEEECCCEEEEEECC
KKTTAVLKAKDRIDLERHYNIKNELKDFGYTYLIFKKKIKKVIRDVKVKLGLKSK
CCEEEEEECCCCCCHHHHCCHHHHHHHCCCCHHHHHHHHHHHHHHHHHHCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 1624462; 8041620; 9278503 [H]