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The map label for this gene is dctA

Identifier: 218697238

GI number: 218697238

Start: 4047322

End: 4048608

Strand: Reverse

Name: dctA

Synonym: EC55989_3975

Alternate gene names: 218697238

Gene position: 4048608-4047322 (Counterclockwise)

Preceding gene: 218697239

Following gene: 218697237

Centisome position: 78.54

GC content: 51.75

Gene sequence:

>1287_bases
ATGAAAACCTCTCTGTTTAAAAGCCTTTACTTTCAGGTCCTGACAGCGATAGCCATTGGTATTCTCCTTGGCCATTTCTA
TCCTGAAATAGGCGAGCAAATGAAACCGCTTGGCGACGGCTTCGTTAAGCTCATTAAGATGATCATCGCTCCTGTCATCT
TTTGTACCGTCGTAACGGGCATTGCGGGCATGGAAAGCATGAAGGCGGTCGGTCGTACCGGCGCAGTCGCACTGCTTTAC
TTTGAAATTGTCAGTACCATCGCGCTGATTATTGGTCTTATCATCGTTAACGTCGTGCAGCCTGGTGCCGGAATGAACGT
CGATCCGGCAACGCTTGATGCGAAAGCGGTAGCGGTTTACGCCGATCAGGCGAAAGACCAGGGCATTGTCGCCTTCATTA
TGGATGTCATCCCGGCGAGCGTCATTGGCGCATTTGCCAGCGGTAACATTCTGCAGGTGCTGCTGTTTGCCGTACTGTTT
GGTTTTGCGCTCCACCGTCTGGGCAGCAAAGGCCAACTGATTTTTAACGTCATCGAAAGTTTCTCGCAGGTCATCTTCGG
TATCATCAATATGATCATGCGTCTGGCGCCTATTGGTGCGTTCGGGGCAATGGCGTTTACCATCGGTAAATACGGCGTCG
GCACACTGGTGCAACTGGGGCAGCTGATTATCTGTTTCTACATTACCTGTATCCTGTTTGTGGTGCTGGTATTGGGTTCA
ATCGCTAAAGCGACTGGTTTCAGTATCTTCAAATTTATCCGCTACATCCGTGAAGAACTGCTGATTGTACTGGGGACTTC
ATCTTCCGAGTCGGCGCTGCCGCGTATGCTCGACAAGATGGAGAAACTCGGCTGCCGCAAATCGGTGGTGGGGCTGGTCA
TCCCGACAGGCTACTCGTTTAACCTTGATGGCACATCGATATACCTGACAATGGCGGCGGTGTTTATCGCCCAGGCCACT
AACAGTCAGATGGATATCGTCCACCAAATCACGCTGTTAATCGTGTTGCTGCTTTCTTCTAAAGGGGCGGCAGGGGTAAC
GGGTAGTGGCTTTATCGTGCTGGCGGCGACGCTCTCTGCGGTGGGCCATTTGCCGGTAGCGGGTCTGGCGCTGATCCTCG
GTATCGACCGCTTTATGTCAGAAGCTCGTGCGCTGACTAACCTGGTCGGTAACGGCGTAGCGACCATTGTCGTTGCTAAG
TGGGTGAAAGAACTGGACCACAAAAAACTGGACGATGTGCTGAATAATCGTGCGCCGGATGGCAAAACGCACGAATTATC
CTCTTAA

Upstream 100 bases:

>100_bases
ATGATCCAGGTACATTTCTGTGATGTTGTCTGGGTGTTATTTTAAGGCCGCAGGTACCCCATAACCTTACAAGACCTGTG
GTTTTACTAAAGGACACCCT

Downstream 100 bases:

>100_bases
TCTCATCACTTATGCCCGCACTCCCTTCCCCGAGTGCGGGTGTTTGCGCATAATTGACCCCTGTGCCTGAACTTACCACC
TGGGTGAGTTCAGGTTTATT

Product: C4-dicarboxylate transporter DctA

Products: orotate [Cytoplasm]; fumarate [Cytoplasm]; malate [Cytoplasm]; Na (I) [Cytoplasm]; succinate [Cytoplasm] [C]

Alternate protein names: NA

Number of amino acids: Translated: 428; Mature: 428

Protein sequence:

>428_residues
MKTSLFKSLYFQVLTAIAIGILLGHFYPEIGEQMKPLGDGFVKLIKMIIAPVIFCTVVTGIAGMESMKAVGRTGAVALLY
FEIVSTIALIIGLIIVNVVQPGAGMNVDPATLDAKAVAVYADQAKDQGIVAFIMDVIPASVIGAFASGNILQVLLFAVLF
GFALHRLGSKGQLIFNVIESFSQVIFGIINMIMRLAPIGAFGAMAFTIGKYGVGTLVQLGQLIICFYITCILFVVLVLGS
IAKATGFSIFKFIRYIREELLIVLGTSSSESALPRMLDKMEKLGCRKSVVGLVIPTGYSFNLDGTSIYLTMAAVFIAQAT
NSQMDIVHQITLLIVLLLSSKGAAGVTGSGFIVLAATLSAVGHLPVAGLALILGIDRFMSEARALTNLVGNGVATIVVAK
WVKELDHKKLDDVLNNRAPDGKTHELSS

Sequences:

>Translated_428_residues
MKTSLFKSLYFQVLTAIAIGILLGHFYPEIGEQMKPLGDGFVKLIKMIIAPVIFCTVVTGIAGMESMKAVGRTGAVALLY
FEIVSTIALIIGLIIVNVVQPGAGMNVDPATLDAKAVAVYADQAKDQGIVAFIMDVIPASVIGAFASGNILQVLLFAVLF
GFALHRLGSKGQLIFNVIESFSQVIFGIINMIMRLAPIGAFGAMAFTIGKYGVGTLVQLGQLIICFYITCILFVVLVLGS
IAKATGFSIFKFIRYIREELLIVLGTSSSESALPRMLDKMEKLGCRKSVVGLVIPTGYSFNLDGTSIYLTMAAVFIAQAT
NSQMDIVHQITLLIVLLLSSKGAAGVTGSGFIVLAATLSAVGHLPVAGLALILGIDRFMSEARALTNLVGNGVATIVVAK
WVKELDHKKLDDVLNNRAPDGKTHELSS
>Mature_428_residues
MKTSLFKSLYFQVLTAIAIGILLGHFYPEIGEQMKPLGDGFVKLIKMIIAPVIFCTVVTGIAGMESMKAVGRTGAVALLY
FEIVSTIALIIGLIIVNVVQPGAGMNVDPATLDAKAVAVYADQAKDQGIVAFIMDVIPASVIGAFASGNILQVLLFAVLF
GFALHRLGSKGQLIFNVIESFSQVIFGIINMIMRLAPIGAFGAMAFTIGKYGVGTLVQLGQLIICFYITCILFVVLVLGS
IAKATGFSIFKFIRYIREELLIVLGTSSSESALPRMLDKMEKLGCRKSVVGLVIPTGYSFNLDGTSIYLTMAAVFIAQAT
NSQMDIVHQITLLIVLLLSSKGAAGVTGSGFIVLAATLSAVGHLPVAGLALILGIDRFMSEARALTNLVGNGVATIVVAK
WVKELDHKKLDDVLNNRAPDGKTHELSS

Specific function: Responsible for the transport of dicarboxylates such as succinate, fumarate, and malate from the periplasm across the membrane [H]

COG id: COG1301

COG function: function code C; Na+/H+-dicarboxylate symporters

Gene ontology:

Cell location: Cell inner membrane; Multi-pass membrane protein [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the sodium:dicarboxylate (SDF) symporter (TC 2.A.23) family [H]

Homologues:

Organism=Homo sapiens, GI5032093, Length=402, Percent_Identity=29.1044776119403, Blast_Score=134, Evalue=2e-31,
Organism=Homo sapiens, GI40254478, Length=466, Percent_Identity=25.5364806866953, Blast_Score=126, Evalue=3e-29,
Organism=Homo sapiens, GI223468566, Length=270, Percent_Identity=32.5925925925926, Blast_Score=125, Evalue=1e-28,
Organism=Homo sapiens, GI223468564, Length=270, Percent_Identity=32.5925925925926, Blast_Score=124, Evalue=1e-28,
Organism=Homo sapiens, GI169790839, Length=474, Percent_Identity=26.5822784810127, Blast_Score=124, Evalue=1e-28,
Organism=Homo sapiens, GI21314632, Length=444, Percent_Identity=25.9009009009009, Blast_Score=124, Evalue=2e-28,
Organism=Homo sapiens, GI4827012, Length=395, Percent_Identity=26.5822784810127, Blast_Score=115, Evalue=7e-26,
Organism=Homo sapiens, GI194239697, Length=466, Percent_Identity=24.0343347639485, Blast_Score=114, Evalue=2e-25,
Organism=Homo sapiens, GI66773030, Length=416, Percent_Identity=24.7596153846154, Blast_Score=111, Evalue=2e-24,
Organism=Homo sapiens, GI262359914, Length=414, Percent_Identity=25.6038647342995, Blast_Score=89, Evalue=6e-18,
Organism=Homo sapiens, GI301601644, Length=205, Percent_Identity=29.2682926829268, Blast_Score=80, Evalue=3e-15,
Organism=Escherichia coli, GI1789947, Length=428, Percent_Identity=100, Blast_Score=848, Evalue=0.0,
Organism=Escherichia coli, GI1790514, Length=414, Percent_Identity=39.8550724637681, Blast_Score=321, Evalue=7e-89,
Organism=Escherichia coli, GI1788024, Length=395, Percent_Identity=23.2911392405063, Blast_Score=97, Evalue=2e-21,
Organism=Escherichia coli, GI1789473, Length=252, Percent_Identity=25.7936507936508, Blast_Score=75, Evalue=8e-15,
Organism=Caenorhabditis elegans, GI17537407, Length=389, Percent_Identity=26.7352185089974, Blast_Score=140, Evalue=1e-33,
Organism=Caenorhabditis elegans, GI71983099, Length=422, Percent_Identity=25.5924170616114, Blast_Score=122, Evalue=4e-28,
Organism=Caenorhabditis elegans, GI71983106, Length=429, Percent_Identity=25.4079254079254, Blast_Score=122, Evalue=5e-28,
Organism=Caenorhabditis elegans, GI71996953, Length=447, Percent_Identity=25.7270693512304, Blast_Score=118, Evalue=6e-27,
Organism=Caenorhabditis elegans, GI193206654, Length=475, Percent_Identity=23.1578947368421, Blast_Score=110, Evalue=2e-24,
Organism=Caenorhabditis elegans, GI193206505, Length=415, Percent_Identity=24.578313253012, Blast_Score=109, Evalue=2e-24,
Organism=Caenorhabditis elegans, GI17541374, Length=441, Percent_Identity=24.7165532879819, Blast_Score=107, Evalue=1e-23,
Organism=Drosophila melanogaster, GI24583025, Length=425, Percent_Identity=23.2941176470588, Blast_Score=115, Evalue=7e-26,
Organism=Drosophila melanogaster, GI17137668, Length=425, Percent_Identity=23.2941176470588, Blast_Score=115, Evalue=7e-26,
Organism=Drosophila melanogaster, GI24583023, Length=425, Percent_Identity=23.2941176470588, Blast_Score=115, Evalue=7e-26,
Organism=Drosophila melanogaster, GI281360481, Length=427, Percent_Identity=24.5901639344262, Blast_Score=89, Evalue=7e-18,
Organism=Drosophila melanogaster, GI281360483, Length=427, Percent_Identity=24.5901639344262, Blast_Score=88, Evalue=9e-18,
Organism=Drosophila melanogaster, GI17137666, Length=427, Percent_Identity=24.5901639344262, Blast_Score=88, Evalue=9e-18,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001991
- InterPro:   IPR018107 [H]

Pfam domain/function: PF00375 SDF [H]

EC number: NA

Molecular weight: Translated: 45437; Mature: 45437

Theoretical pI: Translated: 9.06; Mature: 9.06

Prosite motif: PS00713 NA_DICARBOXYL_SYMP_1 ; PS00714 NA_DICARBOXYL_SYMP_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.9 %Cys     (Translated Protein)
3.5 %Met     (Translated Protein)
4.4 %Cys+Met (Translated Protein)
0.9 %Cys     (Mature Protein)
3.5 %Met     (Mature Protein)
4.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKTSLFKSLYFQVLTAIAIGILLGHFYPEIGEQMKPLGDGFVKLIKMIIAPVIFCTVVTG
CCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCHHHHHHHHHHHHHHHHHHHHHHHHH
IAGMESMKAVGRTGAVALLYFEIVSTIALIIGLIIVNVVQPGAGMNVDPATLDAKAVAVY
HHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCHHHHEEE
ADQAKDQGIVAFIMDVIPASVIGAFASGNILQVLLFAVLFGFALHRLGSKGQLIFNVIES
HHHCCCCHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHH
FSQVIFGIINMIMRLAPIGAFGAMAFTIGKYGVGTLVQLGQLIICFYITCILFVVLVLGS
HHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
IAKATGFSIFKFIRYIREELLIVLGTSSSESALPRMLDKMEKLGCRKSVVGLVIPTGYSF
HHHHHHHHHHHHHHHHHHHHEEEECCCCCHHHHHHHHHHHHHHCCHHHHHEEEEECCEEE
NLDGTSIYLTMAAVFIAQATNSQMDIVHQITLLIVLLLSSKGAAGVTGSGFIVLAATLSA
CCCCHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHCCCCCCCCCCCCHHHHHHHHHH
VGHLPVAGLALILGIDRFMSEARALTNLVGNGVATIVVAKWVKELDHKKLDDVLNNRAPD
HCCCHHHHHHHHHHHHHHHHHHHHHHHHHHCHHHHHHHHHHHHHHHHHHHHHHHCCCCCC
GKTHELSS
CCCCCCCC
>Mature Secondary Structure
MKTSLFKSLYFQVLTAIAIGILLGHFYPEIGEQMKPLGDGFVKLIKMIIAPVIFCTVVTG
CCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCHHHHHHHHHHHHHHHHHHHHHHHHH
IAGMESMKAVGRTGAVALLYFEIVSTIALIIGLIIVNVVQPGAGMNVDPATLDAKAVAVY
HHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCHHHHEEE
ADQAKDQGIVAFIMDVIPASVIGAFASGNILQVLLFAVLFGFALHRLGSKGQLIFNVIES
HHHCCCCHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHH
FSQVIFGIINMIMRLAPIGAFGAMAFTIGKYGVGTLVQLGQLIICFYITCILFVVLVLGS
HHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
IAKATGFSIFKFIRYIREELLIVLGTSSSESALPRMLDKMEKLGCRKSVVGLVIPTGYSF
HHHHHHHHHHHHHHHHHHHHEEEECCCCCHHHHHHHHHHHHHHCCHHHHHEEEEECCEEE
NLDGTSIYLTMAAVFIAQATNSQMDIVHQITLLIVLLLSSKGAAGVTGSGFIVLAATLSA
CCCCHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHCCCCCCCCCCCCHHHHHHHHHH
VGHLPVAGLALILGIDRFMSEARALTNLVGNGVATIVVAKWVKELDHKKLDDVLNNRAPD
HCCCHHHHHHHHHHHHHHHHHHHHHHHHHHCHHHHHHHHHHHHHHHHHHHHHHHCCCCCC
GKTHELSS
CCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: orotate [Periplasm]; fumarate [Periplasm]; malate [Periplasm]; Na (I) [Periplasm]; succinate [Periplasm] [C]

Specific reaction: Na (I) [Periplasm] + orotate [Periplasm] = Na (I) [Cytoplasm] + orotate [Cytoplasm] Na (I) [Periplasm] + fumarate [Periplasm] = Na (I) [Cytoplasm] + fumarate [Cytoplasm] Na (I) [Periplasm] + malate [Periplasm] = Na (I) [Cytoplasm] + malate [Cytoplasm] Na

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: NA