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The map label for this gene is gadX

Identifier: 218697225

GI number: 218697225

Start: 4030094

End: 4030918

Strand: Reverse

Name: gadX

Synonym: EC55989_3961

Alternate gene names: 218697225

Gene position: 4030918-4030094 (Counterclockwise)

Preceding gene: 218697226

Following gene: 218697224

Centisome position: 78.2

GC content: 41.21

Gene sequence:

>825_bases
ATGCAATCATTACATGGGAATTGTCTAATTGCGTACGCAAGACATAAATATATTCTCACCATGGTTAATGGTGAATATCG
CTATTTTAATGGCGGTGACCTGGTTTTTGCGGATGCAAGCCAAATTCGAGTAGATAAGTGTGTTGAAAATTTTGTATTAG
TATCAAGGGATACGCTTTCATTATTTCTGCCGATGCTCAAGGAGGAGGCATTAAATCTTCATGCACATAAAAAAATTTCT
TCATTACTCGTTCATCACTGTAGCAGAGATATTCCTGTTTTTCAGGAAGTTGCGCAACTATCGCAGAATAAGAATCTTCG
CTATGCAGAAATGCTACGTAAAAGAGCATTAATCTTTGCGTTGTTATCTGTTTTTCTTGAGGATGAGCACTTTATACCGC
TGCTTTTGAACGTTTTGCAACCGAACATGCGAACACGAGTTTGTACGGTTATCAATAATAATATCGCCCATGAGTGGACA
CTAGCCCGAATCGCCAGCGAGCTGTTGATGAGTCCAAGCCTGTTAAAGAAAAAATTGCGCGAAGAAGAGACATCATATTC
ACAGTTGCTTACTGAGTGTAGAATGCAACGTGCTTTGCAACTTATTGTTATACATGGCTTTTCAATTAAGCGAGTCGCAG
TGTCCTGTGGATATCACAGCGTGTCGTATTTCATTTACGTCTTTCGAAATTATTATGGGATGACGCCCACAGAGTATCAG
GAGCGATCGGCGCAGGGATTGCCGAACCGTGACTCGGCGGCAAGTATTGTTGCGCAAGGAAATTTTTACGGCACTGACCG
TTCTGCGGAAGGAATAAGATTATAG

Upstream 100 bases:

>100_bases
CTCCCGCTTCGTTTAAATTTATTTATCAATCAATTTGACTTAAGAGGGCGGCGTGCTACATTAATTAACAGTAATATGTT
TATGTAATATTAAGTCAACT

Downstream 100 bases:

>100_bases
AATTTTACTCAGACATAAAAAAAACCCGGCATAGGGGACCGGGAAGAGGATAGTCTGCCGTCTCCAGACTAATAAACCGT
TATAACACTCCCTGTTGGCA

Product: DNA-binding transcriptional regulator GadX

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 274; Mature: 274

Protein sequence:

>274_residues
MQSLHGNCLIAYARHKYILTMVNGEYRYFNGGDLVFADASQIRVDKCVENFVLVSRDTLSLFLPMLKEEALNLHAHKKIS
SLLVHHCSRDIPVFQEVAQLSQNKNLRYAEMLRKRALIFALLSVFLEDEHFIPLLLNVLQPNMRTRVCTVINNNIAHEWT
LARIASELLMSPSLLKKKLREEETSYSQLLTECRMQRALQLIVIHGFSIKRVAVSCGYHSVSYFIYVFRNYYGMTPTEYQ
ERSAQGLPNRDSAASIVAQGNFYGTDRSAEGIRL

Sequences:

>Translated_274_residues
MQSLHGNCLIAYARHKYILTMVNGEYRYFNGGDLVFADASQIRVDKCVENFVLVSRDTLSLFLPMLKEEALNLHAHKKIS
SLLVHHCSRDIPVFQEVAQLSQNKNLRYAEMLRKRALIFALLSVFLEDEHFIPLLLNVLQPNMRTRVCTVINNNIAHEWT
LARIASELLMSPSLLKKKLREEETSYSQLLTECRMQRALQLIVIHGFSIKRVAVSCGYHSVSYFIYVFRNYYGMTPTEYQ
ERSAQGLPNRDSAASIVAQGNFYGTDRSAEGIRL
>Mature_274_residues
MQSLHGNCLIAYARHKYILTMVNGEYRYFNGGDLVFADASQIRVDKCVENFVLVSRDTLSLFLPMLKEEALNLHAHKKIS
SLLVHHCSRDIPVFQEVAQLSQNKNLRYAEMLRKRALIFALLSVFLEDEHFIPLLLNVLQPNMRTRVCTVINNNIAHEWT
LARIASELLMSPSLLKKKLREEETSYSQLLTECRMQRALQLIVIHGFSIKRVAVSCGYHSVSYFIYVFRNYYGMTPTEYQ
ERSAQGLPNRDSAASIVAQGNFYGTDRSAEGIRL

Specific function: Positively regulates the expression of about fifteen genes involved in acid resistance such as gadA, gadB and gadC. Depending on the conditions (growth phase and medium), can repress gadW. Negatively regulates perA expression in acidic conditions and posi

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 HTH araC/xylS-type DNA-binding domain [H]

Homologues:

Organism=Escherichia coli, GI1789933, Length=274, Percent_Identity=98.5401459854015, Blast_Score=560, Evalue=1e-161,
Organism=Escherichia coli, GI1790557, Length=147, Percent_Identity=44.8979591836735, Blast_Score=131, Evalue=5e-32,
Organism=Escherichia coli, GI1786778, Length=194, Percent_Identity=39.6907216494845, Blast_Score=129, Evalue=2e-31,
Organism=Escherichia coli, GI1787776, Length=136, Percent_Identity=43.3823529411765, Blast_Score=103, Evalue=9e-24,
Organism=Escherichia coli, GI1786776, Length=132, Percent_Identity=40.1515151515151, Blast_Score=97, Evalue=2e-21,
Organism=Escherichia coli, GI1789932, Length=128, Percent_Identity=38.28125, Blast_Score=91, Evalue=7e-20,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR009057
- InterPro:   IPR012287
- InterPro:   IPR018062
- InterPro:   IPR020449
- InterPro:   IPR018060 [H]

Pfam domain/function: PF00165 HTH_AraC [H]

EC number: NA

Molecular weight: Translated: 31454; Mature: 31454

Theoretical pI: Translated: 8.93; Mature: 8.93

Prosite motif: PS00041 HTH_ARAC_FAMILY_1 ; PS01124 HTH_ARAC_FAMILY_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.2 %Cys     (Translated Protein)
2.9 %Met     (Translated Protein)
5.1 %Cys+Met (Translated Protein)
2.2 %Cys     (Mature Protein)
2.9 %Met     (Mature Protein)
5.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MQSLHGNCLIAYARHKYILTMVNGEYRYFNGGDLVFADASQIRVDKCVENFVLVSRDTLS
CCCCCCCEEEEEECCEEEEEEECCCEEEECCCCEEEECHHHHHHHHHHHHHHEECCHHHH
LFLPMLKEEALNLHAHKKISSLLVHHCSRDIPVFQEVAQLSQNKNLRYAEMLRKRALIFA
HHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHCCCCHHHHHHHHHHHHHH
LLSVFLEDEHFIPLLLNVLQPNMRTRVCTVINNNIAHEWTLARIASELLMSPSLLKKKLR
HHHHHHCCCCHHHHHHHHHCCCHHHHHHHHHCCCCCHHHHHHHHHHHHHCCHHHHHHHHH
EEETSYSQLLTECRMQRALQLIVIHGFSIKRVAVSCGYHSVSYFIYVFRNYYGMTPTEYQ
HHHHHHHHHHHHHHHHHHHHHHHEECCCHHHHHHHCCHHHHHHHHHHHHHHCCCCCCHHH
ERSAQGLPNRDSAASIVAQGNFYGTDRSAEGIRL
HHHHCCCCCCCCHHHEEECCCEECCCCCCCCCCC
>Mature Secondary Structure
MQSLHGNCLIAYARHKYILTMVNGEYRYFNGGDLVFADASQIRVDKCVENFVLVSRDTLS
CCCCCCCEEEEEECCEEEEEEECCCEEEECCCCEEEECHHHHHHHHHHHHHHEECCHHHH
LFLPMLKEEALNLHAHKKISSLLVHHCSRDIPVFQEVAQLSQNKNLRYAEMLRKRALIFA
HHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHCCCCHHHHHHHHHHHHHH
LLSVFLEDEHFIPLLLNVLQPNMRTRVCTVINNNIAHEWTLARIASELLMSPSLLKKKLR
HHHHHHCCCCHHHHHHHHHCCCHHHHHHHHHCCCCCHHHHHHHHHHHHHCCHHHHHHHHH
EEETSYSQLLTECRMQRALQLIVIHGFSIKRVAVSCGYHSVSYFIYVFRNYYGMTPTEYQ
HHHHHHHHHHHHHHHHHHHHHHHEECCCHHHHHHHCCHHHHHHHHHHHHHHCCCCCCHHH
ERSAQGLPNRDSAASIVAQGNFYGTDRSAEGIRL
HHHHCCCCCCCCHHHEEECCCEECCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA