Click here to switch to the map view.

The map label for this gene is gph

Identifier: 218697065

GI number: 218697065

Start: 3848143

End: 3848901

Strand: Reverse

Name: gph

Synonym: EC55989_3790

Alternate gene names: 218697065

Gene position: 3848901-3848143 (Counterclockwise)

Preceding gene: 218697066

Following gene: 218697064

Centisome position: 74.67

GC content: 52.96

Gene sequence:

>759_bases
ATGAGTAAGTTTGAAGATATTCGCGGCGTCGCTTTTGATCTCGACGGTACGCTGGTGGACAGTGCTCCCGGTCTTGCTGC
TGCGGTAGATATGGCGCTGTATGCGTTGGAGTTACCCGTCGCAGGTGAAGAACGCGTTATTACCTGGATTGGTAACGGCG
CAGATGTTCTGATGGAGCGTGCATTGGCCTGGGCGCGTCAGGAACGTGCGACTCTGCGTAAAACAATGGGTAAACCGCCC
GTTGATGACGACATTCCGGCAGAAGAACAGGTACGTATTCTGCGTAAACTGTTCGATCGCTACTATAGCGAGGTTGCCGA
AGAGGGGACGTTTTTGTTCCCGCACGTTGCCGATACGTTGGGCGCGTTGCAGGCTAAAGGCCTGCCGCTAGGCCTGGTCA
CCAACAAACCGACGCCGTTCGTCGCGCCGCTGCTCGAAGCCTTAGATATTGCCAAATACTTTAGCGTGGTTATCGGCGGC
GATGATGTGCAAAACAAAAAACCGCATCCGGACCCGCTGTTACTGGTGGCTGAGCGGATGGGAATTGCCCCACAAGAGAT
GCTGTTTGTCGGCGACTCACGCAATGATATTCAGGCGGCAAAAGCGGCAGGTTGCCCATCAGTTGGCTTAACCTACGGAT
ATAACTACGGCGAGGCTATCGATCTCAGCCAGCCTGATGTAATTTATCAGTCTATAAATGACCTTCTGCCCGCATTAGGG
CTTCCGCATAGCGAAAATCAGGAATCGAAAAATGACTAA

Upstream 100 bases:

>100_bases
GCGGGCGCGGATATGTTCGTCGCCGGTTCGGCAATCTTCGACCAGCCAGACTATAAAAAAGTCATTGATGAAATGCGCAG
TGAACTGGCAAAGGTAAGTC

Downstream 100 bases:

>100_bases
GCCCATCGTTTTTAGTGGCGCACAGCCCTCAGGTGAATTGACCATTGGTAACTACATGGGTGCGCTGCGTCAGTGGGTAA
ACATGCAGGATGACTACCAT

Product: phosphoglycolate phosphatase

Products: NA

Alternate protein names: PGP; PGPase [H]

Number of amino acids: Translated: 252; Mature: 251

Protein sequence:

>252_residues
MSKFEDIRGVAFDLDGTLVDSAPGLAAAVDMALYALELPVAGEERVITWIGNGADVLMERALAWARQERATLRKTMGKPP
VDDDIPAEEQVRILRKLFDRYYSEVAEEGTFLFPHVADTLGALQAKGLPLGLVTNKPTPFVAPLLEALDIAKYFSVVIGG
DDVQNKKPHPDPLLLVAERMGIAPQEMLFVGDSRNDIQAAKAAGCPSVGLTYGYNYGEAIDLSQPDVIYQSINDLLPALG
LPHSENQESKND

Sequences:

>Translated_252_residues
MSKFEDIRGVAFDLDGTLVDSAPGLAAAVDMALYALELPVAGEERVITWIGNGADVLMERALAWARQERATLRKTMGKPP
VDDDIPAEEQVRILRKLFDRYYSEVAEEGTFLFPHVADTLGALQAKGLPLGLVTNKPTPFVAPLLEALDIAKYFSVVIGG
DDVQNKKPHPDPLLLVAERMGIAPQEMLFVGDSRNDIQAAKAAGCPSVGLTYGYNYGEAIDLSQPDVIYQSINDLLPALG
LPHSENQESKND
>Mature_251_residues
SKFEDIRGVAFDLDGTLVDSAPGLAAAVDMALYALELPVAGEERVITWIGNGADVLMERALAWARQERATLRKTMGKPPV
DDDIPAEEQVRILRKLFDRYYSEVAEEGTFLFPHVADTLGALQAKGLPLGLVTNKPTPFVAPLLEALDIAKYFSVVIGGD
DVQNKKPHPDPLLLVAERMGIAPQEMLFVGDSRNDIQAAKAAGCPSVGLTYGYNYGEAIDLSQPDVIYQSINDLLPALGL
PHSENQESKND

Specific function: Specifically catalyzes the dephosphorylation of 2- phosphoglycolate. Is involved in the dissimilation of the intracellular 2-phosphoglycolate formed during the DNA repair of 3'-phosphoglycolate ends, a major class of DNA lesions induced by oxidative stres

COG id: COG0546

COG function: function code R; Predicted phosphatases

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the HAD-like hydrolase superfamily. CbbY/CbbZ/Gph/YieH family [H]

Homologues:

Organism=Escherichia coli, GI1789787, Length=252, Percent_Identity=98.015873015873, Blast_Score=503, Evalue=1e-144,
Organism=Escherichia coli, GI1787576, Length=211, Percent_Identity=23.696682464455, Blast_Score=62, Evalue=5e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR005834
- InterPro:   IPR023214
- InterPro:   IPR006439
- InterPro:   IPR006402
- InterPro:   IPR005833
- InterPro:   IPR006346
- InterPro:   IPR023198 [H]

Pfam domain/function: PF00702 Hydrolase [H]

EC number: =3.1.3.18 [H]

Molecular weight: Translated: 27348; Mature: 27217

Theoretical pI: Translated: 4.29; Mature: 4.29

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.4 %Cys     (Translated Protein)
2.4 %Met     (Translated Protein)
2.8 %Cys+Met (Translated Protein)
0.4 %Cys     (Mature Protein)
2.0 %Met     (Mature Protein)
2.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSKFEDIRGVAFDLDGTLVDSAPGLAAAVDMALYALELPVAGEERVITWIGNGADVLMER
CCCHHHHCCEEEECCCEEECCCCCHHHHHHHHHHHHHCCCCCCCEEEEEECCCHHHHHHH
ALAWARQERATLRKTMGKPPVDDDIPAEEQVRILRKLFDRYYSEVAEEGTFLFPHVADTL
HHHHHHHHHHHHHHHCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHCCCEECHHHHHHH
GALQAKGLPLGLVTNKPTPFVAPLLEALDIAKYFSVVIGGDDVQNKKPHPDPLLLVAERM
HHHHHCCCCEEEECCCCCCHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCHHHHHHHHH
GIAPQEMLFVGDSRNDIQAAKAAGCPSVGLTYGYNYGEAIDLSQPDVIYQSINDLLPALG
CCCHHHEEEECCCCCHHHHHHHCCCCCCCCEECCCCCCEECCCCCHHHHHHHHHHHHHHC
LPHSENQESKND
CCCCCCCCCCCC
>Mature Secondary Structure 
SKFEDIRGVAFDLDGTLVDSAPGLAAAVDMALYALELPVAGEERVITWIGNGADVLMER
CCHHHHCCEEEECCCEEECCCCCHHHHHHHHHHHHHCCCCCCCEEEEEECCCHHHHHHH
ALAWARQERATLRKTMGKPPVDDDIPAEEQVRILRKLFDRYYSEVAEEGTFLFPHVADTL
HHHHHHHHHHHHHHHCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHCCCEECHHHHHHH
GALQAKGLPLGLVTNKPTPFVAPLLEALDIAKYFSVVIGGDDVQNKKPHPDPLLLVAERM
HHHHHCCCCEEEECCCCCCHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCHHHHHHHHH
GIAPQEMLFVGDSRNDIQAAKAAGCPSVGLTYGYNYGEAIDLSQPDVIYQSINDLLPALG
CCCHHHEEEECCCCCHHHHHHHCCCCCCCCEECCCCCCEECCCCCHHHHHHHHHHHHHHC
LPHSENQESKND
CCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 11206551; 11258796 [H]