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The map label for this gene is ispB

Identifier: 218696892

GI number: 218696892

Start: 3690957

End: 3691928

Strand: Direct

Name: ispB

Synonym: EC55989_3605

Alternate gene names: 218696892

Gene position: 3690957-3691928 (Clockwise)

Preceding gene: 218696887

Following gene: 218696893

Centisome position: 71.6

GC content: 52.47

Gene sequence:

>972_bases
ATGAATTTAGAAAAAATCAATGAGTTAACCGCGCAAGATATGGCGGGTGTTAATGCGGCAATCCTTGAGCAGCTTAATTC
CGACGTCCAACTGATCAATCAGTTAGGCTATTACATCGTCAGCGGCGGCGGTAAACGTATTCGTCCGATGATTGCTGTAC
TGGCTGCACGAGCTGTTGGCTATGAGGGAAATGCGCATGTCACCATTGCTGCCCTGATCGAGTTTATCCACACGGCGACT
CTGCTACACGACGACGTTGTGGATGAATCAGATATGCGCAGGGGTAAAGCTACCGCCAACGCTGCATTTGGCAATGCCGC
CAGCGTGCTGGTAGGCGATTTTATTTATACCCGCGCGTTCCAGATGATGACCAGCCTCGGTTCGCTCAAAGTGCTGGAAG
TCATGTCAGAAGCCGTAAACGTCATCGCAGAAGGTGAAGTTCTGCAACTGATGAACGTTAACGATCCGGACATCACTGAA
GAAAACTACATGCGCGTTATCTATAGCAAAACCGCGCGTCTGTTTGAGGCTGCCGCGCAGTGTTCCGGGATTCTGGCTGG
CTGTACGCCGGAAGAGGAGAAAGGCCTGCAGGATTATGGGCGCTATCTCGGCACTGCTTTCCAGTTGATCGACGATTTAC
TCGATTACAATGCCGATGGCGAACAGTTAGGTAAAAATGTCGGCGACGATCTGAACGAAGGTAAACCGACGCTGCCGCTG
CTGCATGCGATGCATCATGGCACACCAGAACAGGCACAGATGATCCGTACCGCCATCGAACAGGGTAACGGTCGCCATCT
TCTGGAACCGGTTCTGGAAGCAATGAACGCTTGTGGATCTCTTGAATGGACGCGTCAGCGTGCCGAGGAAGAAGCAGACA
AAGCCATCGCAGCATTACAGGTGCTCCCGGACACCCCTTGGCGAGAAGCACTCATCGGCCTCGCGCACATCGCTGTTCAA
CGCGATCGTTAA

Upstream 100 bases:

>100_bases
TGCCATTTTTTCAGTACAATCACCCTATATTCCTAACCATAAACCCTAAGTTGCCTTTGTTCACAGTAAGGTAATCGGGG
CGAAAAGCCCGGCTTTTGCG

Downstream 100 bases:

>100_bases
TCCCCTCCCTTCATCCCGCGCAGTGCGCGGGATGATTCCAGTAAATTCCATAAAAACACTAATTCAGCGCTATAACACCT
GAAAAAAAGGGCTGTGCATC

Product: octaprenyl diphosphate synthase

Products: NA

Alternate protein names: All-trans-octaprenyl-diphosphate synthase; Octaprenyl pyrophosphate synthase; OPP synthase

Number of amino acids: Translated: 323; Mature: 323

Protein sequence:

>323_residues
MNLEKINELTAQDMAGVNAAILEQLNSDVQLINQLGYYIVSGGGKRIRPMIAVLAARAVGYEGNAHVTIAALIEFIHTAT
LLHDDVVDESDMRRGKATANAAFGNAASVLVGDFIYTRAFQMMTSLGSLKVLEVMSEAVNVIAEGEVLQLMNVNDPDITE
ENYMRVIYSKTARLFEAAAQCSGILAGCTPEEEKGLQDYGRYLGTAFQLIDDLLDYNADGEQLGKNVGDDLNEGKPTLPL
LHAMHHGTPEQAQMIRTAIEQGNGRHLLEPVLEAMNACGSLEWTRQRAEEEADKAIAALQVLPDTPWREALIGLAHIAVQ
RDR

Sequences:

>Translated_323_residues
MNLEKINELTAQDMAGVNAAILEQLNSDVQLINQLGYYIVSGGGKRIRPMIAVLAARAVGYEGNAHVTIAALIEFIHTAT
LLHDDVVDESDMRRGKATANAAFGNAASVLVGDFIYTRAFQMMTSLGSLKVLEVMSEAVNVIAEGEVLQLMNVNDPDITE
ENYMRVIYSKTARLFEAAAQCSGILAGCTPEEEKGLQDYGRYLGTAFQLIDDLLDYNADGEQLGKNVGDDLNEGKPTLPL
LHAMHHGTPEQAQMIRTAIEQGNGRHLLEPVLEAMNACGSLEWTRQRAEEEADKAIAALQVLPDTPWREALIGLAHIAVQ
RDR
>Mature_323_residues
MNLEKINELTAQDMAGVNAAILEQLNSDVQLINQLGYYIVSGGGKRIRPMIAVLAARAVGYEGNAHVTIAALIEFIHTAT
LLHDDVVDESDMRRGKATANAAFGNAASVLVGDFIYTRAFQMMTSLGSLKVLEVMSEAVNVIAEGEVLQLMNVNDPDITE
ENYMRVIYSKTARLFEAAAQCSGILAGCTPEEEKGLQDYGRYLGTAFQLIDDLLDYNADGEQLGKNVGDDLNEGKPTLPL
LHAMHHGTPEQAQMIRTAIEQGNGRHLLEPVLEAMNACGSLEWTRQRAEEEADKAIAALQVLPDTPWREALIGLAHIAVQ
RDR

Specific function: Supplies octaprenyl diphosphate, the precursor for the side chain of the isoprenoid quinones ubiquinone and menaquinone

COG id: COG0142

COG function: function code H; Geranylgeranyl pyrophosphate synthase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the FPP/GGPP synthase family

Homologues:

Organism=Homo sapiens, GI50659086, Length=323, Percent_Identity=30.6501547987616, Blast_Score=154, Evalue=1e-37,
Organism=Homo sapiens, GI4758430, Length=278, Percent_Identity=23.021582733813, Blast_Score=78, Evalue=1e-14,
Organism=Homo sapiens, GI83700220, Length=278, Percent_Identity=23.021582733813, Blast_Score=78, Evalue=1e-14,
Organism=Escherichia coli, GI1789578, Length=323, Percent_Identity=100, Blast_Score=662, Evalue=0.0,
Organism=Escherichia coli, GI1786623, Length=254, Percent_Identity=29.5275590551181, Blast_Score=80, Evalue=2e-16,
Organism=Caenorhabditis elegans, GI17505681, Length=351, Percent_Identity=33.3333333333333, Blast_Score=154, Evalue=7e-38,
Organism=Saccharomyces cerevisiae, GI6319475, Length=300, Percent_Identity=33.6666666666667, Blast_Score=149, Evalue=6e-37,
Organism=Saccharomyces cerevisiae, GI6325188, Length=227, Percent_Identity=25.1101321585903, Blast_Score=82, Evalue=9e-17,
Organism=Drosophila melanogaster, GI24651612, Length=298, Percent_Identity=31.5436241610738, Blast_Score=153, Evalue=2e-37,
Organism=Drosophila melanogaster, GI281365769, Length=282, Percent_Identity=21.6312056737589, Blast_Score=72, Evalue=4e-13,
Organism=Drosophila melanogaster, GI24660002, Length=282, Percent_Identity=21.6312056737589, Blast_Score=72, Evalue=4e-13,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): ISPB_ECOLI (P0AD57)

Other databases:

- EMBL:   D13267
- EMBL:   U18997
- EMBL:   U00096
- EMBL:   AP009048
- EMBL:   X68873
- PIR:   E65109
- RefSeq:   AP_003730.1
- RefSeq:   NP_417654.1
- ProteinModelPortal:   P0AD57
- SMR:   P0AD57
- IntAct:   P0AD57
- STRING:   P0AD57
- EnsemblBacteria:   EBESCT00000004959
- EnsemblBacteria:   EBESCT00000017333
- GeneID:   947364
- GenomeReviews:   AP009048_GR
- GenomeReviews:   U00096_GR
- KEGG:   ecj:JW3154
- KEGG:   eco:b3187
- EchoBASE:   EB0017
- EcoGene:   EG10017
- eggNOG:   COG0142
- GeneTree:   EBGT00050000011300
- HOGENOM:   HBG732667
- OMA:   VQQLMNV
- ProtClustDB:   PRK10888
- BioCyc:   EcoCyc:OPPSYN-MONOMER
- BioCyc:   MetaCyc:OPPSYN-MONOMER
- Genevestigator:   P0AD57
- InterPro:   IPR000092
- InterPro:   IPR017446
- InterPro:   IPR008949
- Gene3D:   G3DSA:1.10.600.10
- PANTHER:   PTHR12001

Pfam domain/function: PF00348 polyprenyl_synt; SSF48576 Terpenoid_synth

EC number: =2.5.1.90

Molecular weight: Translated: 35217; Mature: 35217

Theoretical pI: Translated: 4.43; Mature: 4.43

Prosite motif: PS00723 POLYPRENYL_SYNTHASE_1; PS00444 POLYPRENYL_SYNTHASE_2

Important sites: BINDING 45-45 BINDING 48-48 BINDING 77-77 BINDING 93-93 BINDING 94-94 BINDING 170-170 BINDING 171-171 BINDING 208-208

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.9 %Cys     (Translated Protein)
3.7 %Met     (Translated Protein)
4.6 %Cys+Met (Translated Protein)
0.9 %Cys     (Mature Protein)
3.7 %Met     (Mature Protein)
4.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNLEKINELTAQDMAGVNAAILEQLNSDVQLINQLGYYIVSGGGKRIRPMIAVLAARAVG
CCHHHHHHHHHHHHCCCHHHHHHHHCHHHHHHHHHCCEEECCCCCHHHHHHHHHHHHHHC
YEGNAHVTIAALIEFIHTATLLHDDVVDESDMRRGKATANAAFGNAASVLVGDFIYTRAF
CCCCCCCHHHHHHHHHHHHHHHHHHHCCHHHHHHCCHHHHHHCCCHHHHHHHHHHHHHHH
QMMTSLGSLKVLEVMSEAVNVIAEGEVLQLMNVNDPDITEENYMRVIYSKTARLFEAAAQ
HHHHHHCCHHHHHHHHHHHHHHHCCCEEEEECCCCCCCCHHHHHHHHHHHHHHHHHHHHH
CSGILAGCTPEEEKGLQDYGRYLGTAFQLIDDLLDYNADGEQLGKNVGDDLNEGKPTLPL
HCCHHCCCCCHHHCCHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHCCCCCCCCCCCHHH
LHAMHHGTPEQAQMIRTAIEQGNGRHLLEPVLEAMNACGSLEWTRQRAEEEADKAIAALQ
HHHHHCCCHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHH
VLPDTPWREALIGLAHIAVQRDR
HCCCCCHHHHHHHHHHHHHCCCC
>Mature Secondary Structure
MNLEKINELTAQDMAGVNAAILEQLNSDVQLINQLGYYIVSGGGKRIRPMIAVLAARAVG
CCHHHHHHHHHHHHCCCHHHHHHHHCHHHHHHHHHCCEEECCCCCHHHHHHHHHHHHHHC
YEGNAHVTIAALIEFIHTATLLHDDVVDESDMRRGKATANAAFGNAASVLVGDFIYTRAF
CCCCCCCHHHHHHHHHHHHHHHHHHHCCHHHHHHCCHHHHHHCCCHHHHHHHHHHHHHHH
QMMTSLGSLKVLEVMSEAVNVIAEGEVLQLMNVNDPDITEENYMRVIYSKTARLFEAAAQ
HHHHHHCCHHHHHHHHHHHHHHHCCCEEEEECCCCCCCCHHHHHHHHHHHHHHHHHHHHH
CSGILAGCTPEEEKGLQDYGRYLGTAFQLIDDLLDYNADGEQLGKNVGDDLNEGKPTLPL
HCCHHCCCCCHHHCCHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHCCCCCCCCCCCHHH
LHAMHHGTPEQAQMIRTAIEQGNGRHLLEPVLEAMNACGSLEWTRQRAEEEADKAIAALQ
HHHHHCCCHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHH
VLPDTPWREALIGLAHIAVQRDR
HCCCCCHHHHHHHHHHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 8312607; 9278503; 2670911; 8037730