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The map label for this gene is garR

Identifier: 218696830

GI number: 218696830

Start: 3626835

End: 3627725

Strand: Reverse

Name: garR

Synonym: EC55989_3543

Alternate gene names: 218696830

Gene position: 3627725-3626835 (Counterclockwise)

Preceding gene: 218696831

Following gene: 218696829

Centisome position: 70.37

GC content: 53.42

Gene sequence:

>891_bases
ATGACTATGAAAGTTGGTTTTATTGGCCTGGGGATTATGGGTAAACCAATGAGTAAAAACCTTCTGAAAGCAGGTTACTC
GCTGGTGGTTGCTGACCGTAACCCAGAAGCTATTGCTGACGTGATTGCTGCAGGTGCAGAAACAGCGTCTACGGCTAAAG
CGATCGCTGAACAGTGCGACGTCATCATAACCATGCTGCCAAACTCCCCTCATGTGAAAGAGGTGGCGCTGGGTGAGAAT
GGCATCATTGAAGGCGCGAAGCCAGGTACGGTATTGATCGATATGAGTTCTATCGCACCGCTGGCAAGCCGTGAAATCAG
CGAAGCGCTGAAAGCGAAAGGCATTGATATGCTGGATGCTCCGGTGAGCGGCGGTGAACCGAAAGCCATCGACGGTACGC
TGTCAGTGATGGTGGGCGGCGACAAGGCTATTTTCGACAAATACTATGATTTGATGAAAGCGATGGCGGGTTCCGTGGTG
CATACCGGGGAAATCGGTGCAGGTAACGTCACCAAACTGGCAAATCAGGTCATTGTGGCGCTGAATATTGCCGCGATGTC
AGAAGCGCTAACGCTGGCAACCAAAGCGGGCGTTAACCCGGACCTGGTGTATCAGGCAATTCGCGGTGGACTGGCGGGCA
GTACCGTGCTGGATGCCAAAGCGCCGATGGTGATGGACCGCAACTTCAAGCCGGGCTTCCGTATTGATCTGCATATTAAG
GATCTGGCAAATGCGCTGGATACTTCCCACGGCGTCGGCGCGCAACTGCCGCTCACAGCTGCGGTTATGGAGATGATGCA
GGCACTGCGAGCAGATGGTTTAGGAACGGCGGATCATAGCGCCCTGGCGTGCTACTACGAAAAACTGGCGAAAGTCGAAG
TTACTCGTTAA

Upstream 100 bases:

>100_bases
TGGCTGTCGGCAGCGATCTTGGCGTCTTCCGCTCTGCCACTCAGAAACTGGCTGATACCTTTAAAAAATAACCACCACCG
CAACAAGAGAGATGATTGAT

Downstream 100 bases:

>100_bases
TGACGTCGTGCCCGGATGGCGTATTGCAATCCGGGCCTGGAATCCAGGCCGAATAAGAGAAACGCCCTCCGGCGTCATGT
GGTAACAGGCATAGGTATGA

Product: tartronate semialdehyde reductase

Products: NA

Alternate protein names: Tartronate semialdehyde reductase; TSAR [H]

Number of amino acids: Translated: 296; Mature: 295

Protein sequence:

>296_residues
MTMKVGFIGLGIMGKPMSKNLLKAGYSLVVADRNPEAIADVIAAGAETASTAKAIAEQCDVIITMLPNSPHVKEVALGEN
GIIEGAKPGTVLIDMSSIAPLASREISEALKAKGIDMLDAPVSGGEPKAIDGTLSVMVGGDKAIFDKYYDLMKAMAGSVV
HTGEIGAGNVTKLANQVIVALNIAAMSEALTLATKAGVNPDLVYQAIRGGLAGSTVLDAKAPMVMDRNFKPGFRIDLHIK
DLANALDTSHGVGAQLPLTAAVMEMMQALRADGLGTADHSALACYYEKLAKVEVTR

Sequences:

>Translated_296_residues
MTMKVGFIGLGIMGKPMSKNLLKAGYSLVVADRNPEAIADVIAAGAETASTAKAIAEQCDVIITMLPNSPHVKEVALGEN
GIIEGAKPGTVLIDMSSIAPLASREISEALKAKGIDMLDAPVSGGEPKAIDGTLSVMVGGDKAIFDKYYDLMKAMAGSVV
HTGEIGAGNVTKLANQVIVALNIAAMSEALTLATKAGVNPDLVYQAIRGGLAGSTVLDAKAPMVMDRNFKPGFRIDLHIK
DLANALDTSHGVGAQLPLTAAVMEMMQALRADGLGTADHSALACYYEKLAKVEVTR
>Mature_295_residues
TMKVGFIGLGIMGKPMSKNLLKAGYSLVVADRNPEAIADVIAAGAETASTAKAIAEQCDVIITMLPNSPHVKEVALGENG
IIEGAKPGTVLIDMSSIAPLASREISEALKAKGIDMLDAPVSGGEPKAIDGTLSVMVGGDKAIFDKYYDLMKAMAGSVVH
TGEIGAGNVTKLANQVIVALNIAAMSEALTLATKAGVNPDLVYQAIRGGLAGSTVLDAKAPMVMDRNFKPGFRIDLHIKD
LANALDTSHGVGAQLPLTAAVMEMMQALRADGLGTADHSALACYYEKLAKVEVTR

Specific function: D-galactarate metabolism; third step. [C]

COG id: COG2084

COG function: function code I; 3-hydroxyisobutyrate dehydrogenase and related beta-hydroxyacid dehydrogenases

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the 3-hydroxyisobutyrate dehydrogenase family [H]

Homologues:

Organism=Homo sapiens, GI23308751, Length=285, Percent_Identity=35.4385964912281, Blast_Score=168, Evalue=5e-42,
Organism=Homo sapiens, GI40556376, Length=282, Percent_Identity=29.4326241134752, Blast_Score=140, Evalue=2e-33,
Organism=Escherichia coli, GI145693186, Length=294, Percent_Identity=100, Blast_Score=587, Evalue=1e-169,
Organism=Escherichia coli, GI1786719, Length=289, Percent_Identity=43.9446366782007, Blast_Score=240, Evalue=8e-65,
Organism=Escherichia coli, GI1790315, Length=280, Percent_Identity=36.7857142857143, Blast_Score=158, Evalue=5e-40,
Organism=Escherichia coli, GI1789092, Length=282, Percent_Identity=34.3971631205674, Blast_Score=143, Evalue=2e-35,
Organism=Caenorhabditis elegans, GI17557316, Length=275, Percent_Identity=32.7272727272727, Blast_Score=132, Evalue=2e-31,
Organism=Drosophila melanogaster, GI24655230, Length=286, Percent_Identity=32.1678321678322, Blast_Score=142, Evalue=3e-34,
Organism=Drosophila melanogaster, GI19922568, Length=286, Percent_Identity=32.1678321678322, Blast_Score=142, Evalue=3e-34,
Organism=Drosophila melanogaster, GI28574115, Length=282, Percent_Identity=25.531914893617, Blast_Score=108, Evalue=6e-24,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR002204
- InterPro:   IPR015815
- InterPro:   IPR008927
- InterPro:   IPR006115
- InterPro:   IPR013328
- InterPro:   IPR016040
- InterPro:   IPR006183
- InterPro:   IPR006398 [H]

Pfam domain/function: PF03446 NAD_binding_2 [H]

EC number: =1.1.1.60 [H]

Molecular weight: Translated: 30660; Mature: 30529

Theoretical pI: Translated: 5.68; Mature: 5.68

Prosite motif: PS00895 3_HYDROXYISOBUT_DH

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.7 %Cys     (Translated Protein)
5.4 %Met     (Translated Protein)
6.1 %Cys+Met (Translated Protein)
0.7 %Cys     (Mature Protein)
5.1 %Met     (Mature Protein)
5.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTMKVGFIGLGIMGKPMSKNLLKAGYSLVVADRNPEAIADVIAAGAETASTAKAIAEQCD
CEEEEEEEEECCCCCCHHHHHHHCCCEEEEECCCHHHHHHHHHCCCCHHHHHHHHHHHCC
VIITMLPNSPHVKEVALGENGIIEGAKPGTVLIDMSSIAPLASREISEALKAKGIDMLDA
EEEEECCCCCCCEEEEECCCCCCCCCCCCEEEEEHHHCCCHHHHHHHHHHHHCCCCEECC
PVSGGEPKAIDGTLSVMVGGDKAIFDKYYDLMKAMAGSVVHTGEIGAGNVTKLANQVIVA
CCCCCCCCEECCEEEEEECCCHHHHHHHHHHHHHHHCCEEECCCCCCCHHHHHHHHHHHE
LNIAAMSEALTLATKAGVNPDLVYQAIRGGLAGSTVLDAKAPMVMDRNFKPGFRIDLHIK
EHHHHHHHHHHHHHHCCCCHHHHHHHHHCCCCCCEEECCCCCEEECCCCCCCCEEEEEHH
DLANALDTSHGVGAQLPLTAAVMEMMQALRADGLGTADHSALACYYEKLAKVEVTR
HHHHHHCCCCCCCCCCCHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHEECCC
>Mature Secondary Structure 
TMKVGFIGLGIMGKPMSKNLLKAGYSLVVADRNPEAIADVIAAGAETASTAKAIAEQCD
EEEEEEEEECCCCCCHHHHHHHCCCEEEEECCCHHHHHHHHHCCCCHHHHHHHHHHHCC
VIITMLPNSPHVKEVALGENGIIEGAKPGTVLIDMSSIAPLASREISEALKAKGIDMLDA
EEEEECCCCCCCEEEEECCCCCCCCCCCCEEEEEHHHCCCHHHHHHHHHHHHCCCCEECC
PVSGGEPKAIDGTLSVMVGGDKAIFDKYYDLMKAMAGSVVHTGEIGAGNVTKLANQVIVA
CCCCCCCCEECCEEEEEECCCHHHHHHHHHHHHHHHCCEEECCCCCCCHHHHHHHHHHHE
LNIAAMSEALTLATKAGVNPDLVYQAIRGGLAGSTVLDAKAPMVMDRNFKPGFRIDLHIK
EHHHHHHHHHHHHHHCCCCHHHHHHHHHCCCCCCEEECCCCCEEECCCCCCCCEEEEEHH
DLANALDTSHGVGAQLPLTAAVMEMMQALRADGLGTADHSALACYYEKLAKVEVTR
HHHHHHCCCCCCCCCCCHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHEECCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 12471157 [H]